Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,263,137 C→A 100% intergenic (+91/‑321) lpxT → / → mepS lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,263,1370CA97.8% 723.2 / 6.2 228intergenic (+91/‑321)lpxT/mepSlipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base A (109/114);  total (113/115)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 231 total aligned reads displayed.

CACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATC  >  CP009273/2262992‑2263281
                                                                                                                                                 |                                                                                                                                                
   cTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtct                                                                                                                                             <  1:3082817/148‑1 (MQ=255)
    ttttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtctc                                                                                                                                            <  1:1092660/148‑1 (MQ=255)
    ttttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAAAGCAAAAATAATtctc                                                                                                                                            >  2:2461618/1‑148 (MQ=255)
        ttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAg                                                                                                                                        <  1:319999/148‑1 (MQ=255)
           aCAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTaa                                                                                                                                     >  2:2628693/1‑148 (MQ=255)
           aCAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTaa                                                                                                                                     <  2:3320495/148‑1 (MQ=255)
             aaaTCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaat                                                                                                                                   >  2:2056363/1‑148 (MQ=255)
             aaaTCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaat                                                                                                                                   >  2:603290/1‑148 (MQ=255)
              aaTCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatg                                                                                                                                  >  2:1934382/1‑148 (MQ=255)
               aTCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGATTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatga                                                                                                                                 <  1:1780397/148‑1 (MQ=255)
                   cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                             <  2:3447222/148‑1 (MQ=255)
                   cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                             <  1:2209735/148‑1 (MQ=255)
                   cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                             <  1:1250545/148‑1 (MQ=255)
                    tACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGa                                                                                                                            <  1:1270414/148‑1 (MQ=255)
                        aGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGc                                                                                                                        <  1:2795193/148‑1 (MQ=255)
                          gAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGTATGGATTGCAt                                                                                                                      >  1:1941062/1‑148 (MQ=255)
                          gAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCAt                                                                                                                      >  1:142320/1‑148 (MQ=255)
                          gAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAAGGGATTGCAt                                                                                                                      >  2:3034847/1‑148 (MQ=255)
                            aaaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGc                                                                                                                    >  2:1147919/1‑148 (MQ=255)
                             aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCg                                                                                                                   >  1:2358774/1‑148 (MQ=255)
                              aaacaaacATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                  >  1:1258038/1‑148 (MQ=255)
                              aaacaaacATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                  <  1:982163/148‑1 (MQ=255)
                               aacaaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGtt                                                                                                                 >  2:1283818/1‑148 (MQ=255)
                               aacaaacaTTTACAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGtt                                                                                                                 <  2:2749981/148‑1 (MQ=255)
                                acaaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGttt                                                                                                                >  2:1656276/1‑148 (MQ=255)
                                 caaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTc                                                                                                               >  2:889756/1‑148 (MQ=255)
                                        ttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAt                                                                                                        <  2:2795944/148‑1 (MQ=255)
                                         tCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAtt                                                                                                       <  1:1143328/148‑1 (MQ=255)
                                             aaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAc                                                                                                   <  1:926260/148‑1 (MQ=255)
                                             aaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAc                                                                                                   <  1:1005394/148‑1 (MQ=255)
                                              aaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAGTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACt                                                                                                  >  2:92828/1‑148 (MQ=255)
                                               aCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTg                                                                                                      >  1:1729230/1‑143 (MQ=255)
                                               aCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTg                                                                                                      <  2:3487980/143‑1 (MQ=255)
                                                cAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACttt                                                                                                >  2:2486211/1‑148 (MQ=255)
                                                cAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACttt                                                                                                >  1:2862636/1‑148 (MQ=255)
                                                 aaaTAACTCAAAGTTATTAACATCATCAGGGTTTTTTTTATAGTGATGATAATCCTGATGATGCGCACCGTGATTTCATCTATCGAACTCAAAAATAATTCTCTAAGTAAATGTATGGATTGCATGCGTTTCACTCAATTGTACTTTa                                                                                               <  1:205407/148‑1 (MQ=255)
                                                   aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAt                                                                                             <  2:2286374/148‑1 (MQ=255)
                                                   aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAt                                                                                             >  2:663282/1‑148 (MQ=255)
                                                    tAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAtt                                                                                            >  2:3249099/1‑148 (MQ=255)
                                                     aaCTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTg                                                                                           <  1:3438107/148‑1 (MQ=255)
                                                              tAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAcccc                                                                                  >  1:2010527/1‑148 (MQ=255)
                                                               aaTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCg                                                                                 <  1:2308415/148‑1 (MQ=255)
                                                               aaTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCg                                                                                 <  1:341/148‑1 (MQ=255)
                                                               aaTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCg                                                                                 >  2:1535771/1‑148 (MQ=255)
                                                                aTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGc                                                                                >  1:1151187/1‑148 (MQ=255)
                                                                 ttAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCt                                                                               <  2:437438/148‑1 (MQ=255)
                                                                   aaCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCtta                                                                             <  1:1613575/148‑1 (MQ=255)
                                                                   aaCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCtta                                                                             >  1:778665/1‑148 (MQ=255)
                                                                    aCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttat                                                                            <  1:2486211/148‑1 (MQ=255)
                                                                    aCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttat                                                                            <  1:1958325/148‑1 (MQ=255)
                                                                     catcatCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatt                                                                           <  1:2782653/148‑1 (MQ=255)
                                                                        catcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAAc                                                                        <  2:713851/148‑1 (MQ=255)
                                                                        catcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAAc                                                                        >  1:3472162/1‑148 (MQ=255)
                                                                          tcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                      <  1:296896/148‑1 (MQ=255)
                                                                              ggTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTg                                                                  >  1:152936/1‑148 (MQ=255)
                                                                               gTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGt                                                                 <  1:2462315/148‑1 (MQ=255)
                                                                                  aTTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATc                                                              >  2:3049307/1‑148 (MQ=255)
                                                                                    ttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAc                                                            <  1:1818671/148‑1 (MQ=255)
                                                                                    ttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAc                                                            >  2:1905904/1‑148 (MQ=255)
                                                                                    ttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAc                                                            <  1:1283818/148‑1 (MQ=255)
                                                                                     tttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACt                                                           >  2:529067/1‑148 (MQ=255)
                                                                                     tttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACt                                                           <  1:3230012/148‑1 (MQ=255)
                                                                                     tttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACt                                                           <  1:2474297/148‑1 (MQ=255)
                                                                                        ataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACtttt                                                        <  2:1353486/148‑1 (MQ=255)
                                                                                        ataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACtttt                                                        <  2:157857/148‑1 (MQ=255)
                                                                                          aGTGCGGATAATCCTCATGAAGCGCACCGTGCATTAATCTATCGAAAGAAAAAATAATTCTCTAAGTAAATGAATGGATTGCTTTCGTTTCAATCACATGAACTTTAATTGACCAACCCCGCTGATTAACTGTCTGTATCACtatatc                                                      >  2:2778951/1‑143 (MQ=255)
                                                                                          aGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTc                                                      <  2:411916/148‑1 (MQ=255)
                                                                                           gTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCt                                                     >  1:618045/1‑148 (MQ=255)
                                                                                            tGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCtt                                                    <  1:2137650/148‑1 (MQ=255)
                                                                                             gAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTta                                                   >  1:3037529/1‑148 (MQ=255)
                                                                                              aGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTtat                                                  <  2:2406416/148‑1 (MQ=255)
                                                                                               ggATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTtata                                                 >  1:2122811/1‑148 (MQ=255)
                                                                                                gATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaa                                                <  1:2559117/148‑1 (MQ=255)
                                                                                                 aTAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaa                                               <  2:1381813/148‑1 (MQ=255)
                                                                                                   aaTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaa                                             >  2:2239350/1‑148 (MQ=255)
                                                                                                    aTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaaa                                            <  2:3152579/148‑1 (MQ=255)
                                                                                                          atgatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGt                                      >  1:3109581/1‑148 (MQ=255)
                                                                                                          atgatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATAATGt                                      >  2:2098330/1‑148 (MQ=255)
                                                                                                           tgatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTa                                     >  1:1963983/1‑148 (MQ=255)
                                                                                                             atgCTCACCGTTCTTTCATATATCGAACGCAAAAATAATTCTCTAAGTAAATGTATGGTTTTCATGAGTTTCACTCAATTGTTCTTTAATTGACCAACCCCGCTTATTAATTTTCTGTATCACTGCTTCTTATTAAAAATCATGTaaa                                   <  2:1627498/148‑1 (MQ=255)
                                                                                                             atgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaa                                   <  1:603290/148‑1 (MQ=255)
                                                                                                             atgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaa                                   <  1:842896/148‑1 (MQ=255)
                                                                                                              tgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaaa                                  >  2:2216335/1‑148 (MQ=255)
                                                                                                                  cACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGc                              <  2:1790565/148‑1 (MQ=255)
                                                                                                                       tGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGcc                         >  2:3521343/1‑148 (MQ=255)
                                                                                                                        gCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCa                        >  1:1187378/1‑148 (MQ=255)
                                                                                                                        gCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCa                        >  2:3195764/1‑148 (MQ=255)
                                                                                                                                cTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCa                <  2:3417512/148‑1 (MQ=255)
                                                                                                                                cTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCa                >  2:2917353/1‑148 (MQ=255)
                                                                                                                                cTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCa                >  2:1691418/1‑148 (MQ=255)
                                                                                                                                   tCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCa             >  2:2537165/1‑148 (MQ=255)
                                                                                                                                   tCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCa             >  1:1454128/1‑148 (MQ=255)
                                                                                                                                    cGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCaa            >  1:1327877/1‑148 (MQ=255)
                                                                                                                                      aaCGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATc          >  1:2913056/1‑148 (MQ=255)
                                                                                                                                          cAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGt      <  1:66538/148‑1 (MQ=255)
                                                                                                                                           aaaaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTa     >  1:219227/1‑148 (MQ=255)
                                                                                                                                             aaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAt   >  1:3090975/1‑148 (MQ=255)
                                                                                                                                             aaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAt   >  1:748157/1‑148 (MQ=255)
                                                                                                                                             aaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAt   >  2:1171843/1‑148 (MQ=255)
                                                                                                                                              aataatTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtc  <  1:2519038/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATC  >  CP009273/2262992‑2263281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAA  >  CP009273/2262988‑2263286
                                                                                                                                                     |                                                                                                                                                     
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATA                                                                                                                                                       >  SRR19973067.2896530/1‑150 (MQ=60)
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATA                                                                                                                                                       >  SRR19973067.1532423/1‑150 (MQ=60)
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATA                                                                                                                                                       >  SRR19973067.3305629/1‑150 (MQ=60)
  ATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAAT                                                                                                                                                     >  SRR19973067.112094/1‑150 (MQ=60)
  ATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAAT                                                                                                                                                     <  SRR19973067.948622/150‑1 (MQ=60)
  ATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAAT                                                                                                                                                     <  SRR19973067.2736200/150‑1 (MQ=60)
   TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATT                                                                                                                                                    <  SRR19973067.564731/150‑1 (MQ=60)
       CTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCT                                                                                                                                                <  SRR19973067.3102436/150‑1 (MQ=60)
       CTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCT                                                                                                                                                <  SRR19973067.3095241/150‑1 (MQ=60)
        TTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTA                                                                                                                                               <  SRR19973067.1100119/150‑1 (MQ=60)
         TTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAA                                                                                                                                              >  SRR19973067.3107719/1‑150 (MQ=60)
          TTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAG                                                                                                                                             <  SRR19973067.1162854/150‑1 (MQ=60)
           TTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGT                                                                                                                                            <  SRR19973067.757275/150‑1 (MQ=60)
            TTGACAAATCACGACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTA                                                                                                                                           <  SRR19973067.322167/150‑1 (MQ=60)
                 AAATCACTACCATGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGA                                                                                                                                      <  SRR19973067.3055557/150‑1 (MQ=60)
                 AAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGA                                                                                                                                      >  SRR19973067.803535/1‑150 (MQ=60)
                   ATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGATTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAAT                                                                                                                                    <  SRR19973067.1792437/150‑1 (MQ=60)
                     CACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGG                                                                                                                                  <  SRR19973067.1875853/150‑1 (MQ=60)
                       CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                                <  SRR19973067.1259016/150‑1 (MQ=60)
                       CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                                <  SRR19973067.1258990/150‑1 (MQ=60)
                       CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                                <  SRR19973067.1258777/150‑1 (MQ=60)
                       CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                                <  SRR19973067.2224466/150‑1 (MQ=60)
                       CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                                <  SRR19973067.2224851/150‑1 (MQ=60)
                        TACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATT                                                                                                                               <  SRR19973067.1279053/150‑1 (MQ=60)
                         ACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTG                                                                                                                              <  SRR19973067.1786124/150‑1 (MQ=60)
                         ACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTG                                                                                                                              <  SRR19973067.2140136/150‑1 (MQ=60)
                         ACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTG                                                                                                                              <  SRR19973067.1060292/150‑1 (MQ=60)
                           CAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCA                                                                                                                            <  SRR19973067.1874887/150‑1 (MQ=60)
                            AGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGTATGGATTGCAT                                                                                                                           >  SRR19973067.1954297/1‑150 (MQ=60)
                            AGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCAT                                                                                                                           >  SRR19973067.143575/1‑150 (MQ=60)
                            AGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCAT                                                                                                                           <  SRR19973067.2813225/150‑1 (MQ=60)
                            AGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCAT                                                                                                                           >  SRR19973067.1792992/1‑150 (MQ=60)
                               AAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCG                                                                                                                        >  SRR19973067.2374153/1‑150 (MQ=60)
                                AAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGT                                                                                                                       >  SRR19973067.1266557/1‑150 (MQ=60)
                                  AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                     <  SRR19973067.988904/150‑1 (MQ=60)
                                     CAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCAC                                                                                                                  >  SRR19973067.2214200/1‑150 (MQ=60)
                                      AAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACT                                                                                                                 >  SRR19973067.909251/1‑150 (MQ=60)
                                          ATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAT                                                                                                             <  SRR19973067.1964813/150‑1 (MQ=60)
                                             TCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGT                                                                                                          <  SRR19973067.1151000/150‑1 (MQ=60)
                                              CCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTA                                                                                                         >  SRR19973067.1624542/1‑150 (MQ=60)
                                                 AAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAaga                                                                                                      >  SRR19973067.1741070/1‑147 (MQ=60)
                                                 AAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAaga                                                                                                      >  SRR19973067.3510115/1‑147 (MQ=60)
                                                 AAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTT                                                                                                      <  SRR19973067.1012298/150‑1 (MQ=60)
                                                 AAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTT                                                                                                      <  SRR19973067.2798656/150‑1 (MQ=60)
                                                 AAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTT                                                                                                      <  SRR19973067.932499/150‑1 (MQ=60)
                                                  AACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTT                                                                                                     >  SRR19973067.2880982/1‑150 (MQ=60)
                                                     AAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAT                                                                                                  <  SRR19973067.1468701/150‑1 (MQ=60)
                                                     AAATAACTCAAAGTTATTAACATCATCAGGGTTTTTTTTATAGTGATGATAATCCTGATGATGCGCACCGTGATTTCATCTATCGAACTCAAAAATAATTCTCTAAGTAAATGTATGGATTGCATGCGTTTCACTCAATTGTACTTTAAT                                                                                                  <  SRR19973067.206957/150‑1 (MQ=60)
                                                      AATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAANT                                                                                                 <  SRR19973067.2926175/150‑1 (MQ=60)
                                                         AACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAC                                                                                              <  SRR19973067.3459998/150‑1 (MQ=60)
                                                                AGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCC                                                                                       >  SRR19973067.2024059/1‑150 (MQ=60)
                                                                AGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCC                                                                                       >  SRR19973067.2024136/1‑150 (MQ=60)
                                                                AGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCC                                                                                       >  SRR19973067.951499/1‑150 (MQ=60)
                                                                  TAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGC                                                                                     >  SRR19973067.1158763/1‑150 (MQ=60)
                                                                  TAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGC                                                                                     >  SRR19973067.1158887/1‑150 (MQ=60)
                                                                  TAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGC                                                                                     >  SRR19973067.2650336/1‑150 (MQ=60)
                                                                   AATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACTCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACAAACCCAGCT                                                                                    >  SRR19973067.795189/1‑150 (MQ=60)
                                                                   AATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCT                                                                                    <  SRR19973067.348/150‑1 (MQ=60)
                                                                   AATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCT                                                                                    <  SRR19973067.2323623/150‑1 (MQ=60)
                                                                   AATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCT                                                                                    >  SRR19973067.3310133/1‑150 (MQ=60)
                                                                     TTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTA                                                                                  >  SRR19973067.784057/1‑150 (MQ=60)
                                                                       AACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATT                                                                                <  SRR19973067.1624652/150‑1 (MQ=60)
                                                                        ACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTA                                                                               <  SRR19973067.1971641/150‑1 (MQ=60)
                                                                        ACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTA                                                                               <  SRR19973067.2502550/150‑1 (MQ=60)
                                                                        ACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTA                                                                               <  SRR19973067.2501978/150‑1 (MQ=60)
                                                                         CATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAA                                                                              <  SRR19973067.2800611/150‑1 (MQ=60)
                                                                         CATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAA                                                                              <  SRR19973067.2803113/150‑1 (MQ=60)
                                                                          ATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAAC                                                                             >  SRR19973067.3494217/1‑150 (MQ=60)
                                                                          ATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAAC                                                                             >  SRR19973067.3494207/1‑150 (MQ=60)
                                                                           TCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACT                                                                            <  SRR19973067.2979092/150‑1 (MQ=60)
                                                                           TCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACT                                                                            <  SRR19973067.718136/150‑1 (MQ=60)
                                                                            CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                           >  SRR19973067.290945/1‑150 (MQ=60)
                                                                             ATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTT                                                                          <  SRR19973067.3478634/150‑1 (MQ=60)
                                                                             ATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTT                                                                          <  SRR19973067.2139583/150‑1 (MQ=60)
                                                                              TCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTC                                                                         <  SRR19973067.298789/150‑1 (MQ=60)
                                                                               CAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCT                                                                        <  SRR19973067.1598821/150‑1 (MQ=60)
                                                                                AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                       <  SRR19973067.1316008/150‑1 (MQ=60)
                                                                                AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                       >  SRR19973067.154232/1‑150 (MQ=60)
                                                                                   GTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAT                                                                    <  SRR19973067.2479090/150‑1 (MQ=60)
                                                                                   GTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAT                                                                    <  SRR19973067.2478500/150‑1 (MQ=60)
                                                                                     TATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCA                                                                  >  SRR19973067.1987117/1‑150 (MQ=60)
                                                                                        TTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTT                                                               <  SRR19973067.1830988/150‑1 (MQ=60)
                                                                                        TTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTT                                                               <  SRR19973067.1291642/150‑1 (MQ=60)
                                                                                        TTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTT                                                               <  SRR19973067.1292568/150‑1 (MQ=60)
                                                                                         TTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTT                                                              <  SRR19973067.3250286/150‑1 (MQ=60)
                                                                                         TTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTT                                                              <  SRR19973067.2490564/150‑1 (MQ=60)
                                                                                           TATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTT                                                            <  SRR19973067.2497602/150‑1 (MQ=60)
                                                                                             TAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCT                                                          >  SRR19973067.621785/1‑150 (MQ=60)
                                                                                             TAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCT                                                          >  SRR19973067.622098/1‑150 (MQ=60)
                                                                                               GTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTA                                                        >  SRR19973067.3056879/1‑150 (MQ=60)
                                                                                                TGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTAT                                                       <  SRR19973067.2152026/150‑1 (MQ=60)
                                                                                                TGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTAT                                                       <  SRR19973067.2151932/150‑1 (MQ=60)
                                                                                                TGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTAT                                                       <  SRR19973067.2430714/150‑1 (MQ=60)
                                                                                                 GAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATA                                                      >  SRR19973067.2136975/1‑150 (MQ=60)
                                                                                                    GATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAA                                                   <  SRR19973067.2575844/150‑1 (MQ=60)
                                                                                                     ATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAA                                                  >  SRR19973067.2526426/1‑150 (MQ=60)
                                                                                                       AATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAAT                                                <  SRR19973067.2233443/150‑1 (MQ=60)
                                                                                                           CTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATG                                            >  SRR19973067.3227662/1‑150 (MQ=60)
                                                                                                            TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                           >  SRR19973067.3131320/1‑150 (MQ=60)
                                                                                                            TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                           <  SRR19973067.134188/150‑1 (MQ=60)
                                                                                                            TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                           >  SRR19973067.3129362/1‑150 (MQ=60)
                                                                                                            TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                           >  SRR19973067.3129361/1‑150 (MQ=60)
                                                                                                            TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                           <  SRR19973067.1992375/150‑1 (MQ=60)
                                                                                                            TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                           >  SRR19973067.3077459/1‑150 (MQ=60)
                                                                                                             GATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTA                                          >  SRR19973067.644407/1‑150 (MQ=60)
                                                                                                             GATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTA                                          >  SRR19973067.1977121/1‑150 (MQ=60)
                                                                                                             GATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTA                                          >  SRR19973067.170787/1‑150 (MQ=60)
                                                                                                             GATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTA                                          >  SRR19973067.1977321/1‑150 (MQ=60)
                                                                                                              ATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAA                                         >  SRR19973067.644395/1‑150 (MQ=60)
                                                                                                                 ATGCGCACCGTGCTTTCATCTATCGAACTCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAC                                      <  SRR19973067.607121/150‑1 (MQ=60)
                                                                                                                 ATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAC                                      <  SRR19973067.848612/150‑1 (MQ=60)
                                                                                                                 ATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAC                                      <  SRR19973067.3441762/150‑1 (MQ=60)
                                                                                                                 ATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAC                                      <  SRR19973067.2070071/150‑1 (MQ=60)
                                                                                                                  TGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACC                                     <  SRR19973067.2536155/150‑1 (MQ=60)
                                                                                                                     GCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCT                                  <  SRR19973067.135702/150‑1 (MQ=60)
                                                                                                                      CACCGTGCTTTCATCTATCGACCTCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTC                                 <  SRR19973067.2355998/150‑1 (MQ=60)
                                                                                                                      CACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTC                                 <  SRR19973067.1267042/150‑1 (MQ=60)
                                                                                                                      CACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTC                                 <  SRR19973067.1267010/150‑1 (MQ=60)
                                                                                                                          GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCA                             >  SRR19973067.1195072/1‑150 (MQ=60)
                                                                                                                          GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCA                             >  SRR19973067.1195197/1‑150 (MQ=60)
                                                                                                                                     TATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCA                  >  SRR19973067.1463860/1‑150 (MQ=60)
                                                                                                                                     TATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCA                  >  SRR19973067.3209422/1‑150 (MQ=60)
                                                                                                                                     TATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCA                  >  SRR19973067.1463827/1‑150 (MQ=60)
                                                                                                                                      ATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAA                 >  SRR19973067.1336937/1‑150 (MQ=60)
                                                                                                                                        CGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATC               >  SRR19973067.2931606/1‑150 (MQ=60)
                                                                                                                                             GCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTA          >  SRR19973067.220836/1‑150 (MQ=60)
                                                                                                                                              CAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAA         <  SRR19973067.67326/150‑1 (MQ=60)
                                                                                                                                               AAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAT        >  SRR19973067.753346/1‑150 (MQ=60)
                                                                                                                                               AAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAT        >  SRR19973067.3110635/1‑150 (MQ=60)
                                                                                                                                               AAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAT        >  SRR19973067.284149/1‑150 (MQ=60)
                                                                                                                                                 AAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCT      >  SRR19973067.1531302/1‑150 (MQ=60)
                                                                                                                                                  AATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC     >  SRR19973067.2354447/1‑150 (MQ=60)
                                                                                                                                                  AATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC     <  SRR19973067.2535555/150‑1 (MQ=60)
                                                                                                                                                   ATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCG    >  SRR19973067.128767/1‑150 (MQ=60)
                                                                                                                                                   ATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCG    >  SRR19973067.955633/1‑150 (MQ=60)
                                                                                                                                                   ATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCG    >  SRR19973067.971601/1‑150 (MQ=60)
                                                                                                                                                   ATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCG    >  SRR19973067.974560/1‑150 (MQ=60)
                                                                                                                                                     AATTCTATAAGTAAATGAATGGATTGCATGAGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAA  <  SRR19973067.3319979/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGAA  >  CP009273/2262988‑2263286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑