Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,263,137 C→A 42.9% intergenic (+91/‑321) lpxT → / → mepS lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,263,1370CA42.9% 137.9 / 206.3 163intergenic (+91/‑321)lpxT/mepSlipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (53/40);  new base A (46/24);  total (99/64)
Fisher's exact test for biased strand distribution p-value = 3.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

Only 100 of 164 total aligned reads displayed.

AATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  >  CP009273/2262989‑2263283
                                                                                                                                                    |                                                                                                                                                  
aaTCACATTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCa                                                                                                                                                   <  1:858601/150‑1 (MQ=255)
 aTCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGTTAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCAt                                                                                                                                                  <  1:197923/150‑1 (MQ=255)
   cACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATtc                                                                                                                                                <  2:4135350/150‑1 (MQ=255)
        tttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTaa                                                                                                                                           <  2:549358/150‑1 (MQ=255)
           ttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTa                                                                                                                                        >  1:2695798/1‑150 (MQ=255)
              aCAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAaat                                                                                                                                     >  1:1207916/1‑150 (MQ=255)
               cAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatg                                                                                                                                    >  2:871727/1‑150 (MQ=255)
                 aaTCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAaatgaa                                                                                                                                  <  2:3983152/150‑1 (MQ=255)
                  aTCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAaatgaat                                                                                                                                 <  1:3430027/150‑1 (MQ=255)
                   tCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaatg                                                                                                                                >  1:3148610/1‑150 (MQ=255)
                     aCTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGa                                                                                                                              >  2:250805/1‑150 (MQ=255)
                     aCTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGa                                                                                                                              >  2:250606/1‑150 (MQ=255)
                      cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAt                                                                                                                             >  2:60973/1‑150 (MQ=255)
                           aGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCAt                                                                                                                        >  2:2979625/1‑150 (MQ=255)
                           aGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCAt                                                                                                                        <  2:925186/150‑1 (MQ=255)
                           aGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCAt                                                                                                                        <  2:4715540/150‑1 (MQ=255)
                            ggAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATg                                                                                                                       >  2:2783855/1‑150 (MQ=255)
                              aaaaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAGTGCATGCg                                                                                                                     >  2:2642501/1‑150 (MQ=255)
                               aaaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                    >  2:525552/1‑150 (MQ=255)
                               aaaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                    >  1:825972/1‑150 (MQ=255)
                                aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGtt                                                                                                                   <  1:855514/150‑1 (MQ=255)
                                aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGtt                                                                                                                   >  1:4086397/1‑150 (MQ=255)
                                 aaacaaacATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGttt                                                                                                                  <  1:4639069/150‑1 (MQ=255)
                                 aaacaaacATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGttt                                                                                                                  <  1:2623065/150‑1 (MQ=255)
                                   acaaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCa                                                                                                                <  2:723750/150‑1 (MQ=255)
                                    caaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCAc                                                                                                               <  1:3890533/150‑1 (MQ=255)
                                        caTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCaa                                                                                                           >  1:2422798/1‑150 (MQ=255)
                                         aTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAt                                                                                                          >  1:1543523/1‑150 (MQ=255)
                                         aTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAt                                                                                                          >  1:1543541/1‑150 (MQ=255)
                                          tttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAtt                                                                                                         <  2:868491/150‑1 (MQ=255)
                                          tttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTAGAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAtt                                                                                                         <  2:4669602/150‑1 (MQ=255)
                                           ttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTg                                                                                                        >  2:365691/1‑150 (MQ=255)
                                            tCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGt                                                                                                       >  1:3211973/1‑150 (MQ=255)
                                            tCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGt                                                                                                       <  1:812465/150‑1 (MQ=255)
                                             ccAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTa                                                                                                      >  2:3220829/1‑150 (MQ=255)
                                               aaaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACt                                                                                                    >  2:1808954/1‑150 (MQ=255)
                                               aaaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACt                                                                                                    >  2:2406987/1‑150 (MQ=255)
                                                aaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACtt                                                                                                   >  1:3897956/1‑150 (MQ=255)
                                                      aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTg                                                                                             >  2:2781499/1‑150 (MQ=255)
                                                      aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTg                                                                                             <  2:441252/150‑1 (MQ=255)
                                                       tAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTCCACTCAATTGTACTTTAATTGa                                                                                            <  2:738593/150‑1 (MQ=255)
                                                         aCTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAcc                                                                                          >  1:4375591/1‑150 (MQ=255)
                                                          cTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCa                                                                                         >  2:786754/1‑150 (MQ=255)
                                                               aGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAcccc                                                                                    >  2:2795892/1‑150 (MQ=255)
                                                               aGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGC‑AAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCg                                                                                   <  2:1576004/150‑1 (MQ=255)
                                                                gTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCg                                                                                   >  1:2669805/1‑150 (MQ=255)
                                                                 tAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGc                                                                                  <  2:2577183/150‑1 (MQ=255)
                                                                 tAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGc                                                                                  <  2:2577117/150‑1 (MQ=255)
                                                                 tAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGc                                                                                  <  2:3119267/150‑1 (MQ=255)
                                                                  aaTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCt                                                                                 >  1:2949096/1‑150 (MQ=255)
                                                                  aaTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCt                                                                                 >  2:1108028/1‑150 (MQ=255)
                                                                  aaTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCt                                                                                 >  2:1108090/1‑150 (MQ=255)
                                                                   aTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCtt                                                                                >  2:3141749/1‑150 (MQ=255)
                                                                    ttAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCtta                                                                               <  2:3622400/150‑1 (MQ=255)
                                                                     tAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttat                                                                              >  1:1632270/1‑150 (MQ=255)
                                                                           catcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                        <  2:3128128/150‑1 (MQ=255)
                                                                           catcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                        >  2:2987137/1‑150 (MQ=255)
                                                                              caGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCt                                                                     >  1:4299322/1‑150 (MQ=255)
                                                                              caGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCt                                                                     >  1:1999617/1‑150 (MQ=255)
                                                                              caGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCt                                                                     >  1:2006984/1‑150 (MQ=255)
                                                                              caGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCt                                                                     >  2:716844/1‑150 (MQ=255)
                                                                               aGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTg                                                                    <  2:1898952/150‑1 (MQ=255)
                                                                                  gTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAt                                                                 >  1:3389837/1‑150 (MQ=255)
                                                                                      tttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACt                                                             >  1:2542284/1‑150 (MQ=255)
                                                                                      tttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACt                                                             >  1:1927211/1‑150 (MQ=255)
                                                                                          tataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACttttt                                                         >  2:394682/1‑150 (MQ=255)
                                                                                          tataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACttttt                                                         >  2:2246272/1‑150 (MQ=255)
                                                                                           ataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCa                                                               <  1:82489/143‑1 (MQ=255)
                                                                                           ataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCa                                                               >  2:82489/1‑143 (MQ=255)
                                                                                              gTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTta                                                     >  2:2426943/1‑150 (MQ=255)
                                                                                              gTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTATAAGTATATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTta                                                     <  1:3174846/150‑1 (MQ=255)
                                                                                              gTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTta                                                     >  2:214822/1‑150 (MQ=255)
                                                                                                gAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTtata                                                   >  1:2951622/1‑150 (MQ=255)
                                                                                                gAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTtata                                                   <  2:1213971/150‑1 (MQ=255)
                                                                                                  ggATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaa                                                 <  2:3391821/150‑1 (MQ=255)
                                                                                                   gATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaa                                                >  1:1176107/1‑150 (MQ=255)
                                                                                                   gATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaa                                                <  2:1542231/150‑1 (MQ=255)
                                                                                                    aTAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaa                                               >  2:3610664/1‑150 (MQ=255)
                                                                                                    aTAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaa                                               <  1:3766426/150‑1 (MQ=255)
                                                                                                       aTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATc                                            <  2:1941281/150‑1 (MQ=255)
                                                                                                       aTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATc                                            >  1:2767627/1‑150 (MQ=255)
                                                                                                          cTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATg                                         >  1:711254/1‑150 (MQ=255)
                                                                                                          cTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATg                                         >  2:1701140/1‑150 (MQ=255)
                                                                                                                atgCGCACCGTGCTTACATCTATCGAACGCAAAAATCATTCTCTAAGAAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAc                                   <  1:3959360/150‑1 (MQ=255)
                                                                                                                 tgCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAcc                                  <  1:4050333/150‑1 (MQ=255)
                                                                                                                     cACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTc                              <  1:717786/150‑1 (MQ=255)
                                                                                                                        cGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGcc                           >  1:3741412/1‑150 (MQ=255)
                                                                                                                         gTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCa                          <  2:1977125/150‑1 (MQ=255)
                                                                                                                         gTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCa                          <  2:825972/150‑1 (MQ=255)
                                                                                                                          tGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCaa                         <  1:1798411/150‑1 (MQ=255)
                                                                                                                           gCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAg                        >  2:2652809/1‑150 (MQ=255)
                                                                                                                                    tATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTCCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCa               >  2:3535778/1‑150 (MQ=255)
                                                                                                                                     aTCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTCCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCaa              >  1:3134671/1‑150 (MQ=255)
                                                                                                                                      tCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAAt             >  2:2926716/1‑150 (MQ=255)
                                                                                                                                          aCGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCggg         <  2:4148132/150‑1 (MQ=255)
                                                                                                                                            gCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTa       <  1:4755294/150‑1 (MQ=255)
                                                                                                                                             cAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTaa      >  1:368462/1‑150 (MQ=255)
                                                                                                                                               aaaaTCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtc    >  2:2492643/1‑150 (MQ=255)
                                                                                                                                               aaaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTAATAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtc    >  2:3886262/1‑150 (MQ=255)
                                                                                                                                                 aaTCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtctc  >  2:1045594/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  >  CP009273/2262989‑2263283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A