Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,263,137 C→A 100% intergenic (+91/‑321) lpxT → / → mepS lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,263,1370CA98.3% 717.9 / 3.1 229intergenic (+91/‑321)lpxT/mepSlipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (111/114);  total (114/115)
Fisher's exact test for biased strand distribution p-value = 3.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATC  >  CP009273/2262991‑2263281
                                                                                                                                                  |                                                                                                                                                
 cACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAaataat                                                                                                                                                >  1:352126/1‑148 (MQ=255)
   ccTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtc                                                                                                                                              >  1:509384/1‑148 (MQ=255)
    cTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtct                                                                                                                                             <  2:2770056/148‑1 (MQ=255)
     ttttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtctc                                                                                                                                            >  1:625020/1‑148 (MQ=255)
      tttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTctct                                                                                                                                           >  1:2869162/1‑148 (MQ=255)
          tGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGt                                                                                                                                       <  2:493945/148‑1 (MQ=255)
            aCAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTaa                                                                                                                                     <  2:1063768/148‑1 (MQ=255)
            aCAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTaa                                                                                                                                     <  2:1065774/148‑1 (MQ=255)
             cAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTaaa                                                                                                                                    <  1:1018815/148‑1 (MQ=255)
              aaaTCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaat                                                                                                                                   >  2:2359356/1‑148 (MQ=255)
                aTCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatga                                                                                                                                 <  2:2989099/148‑1 (MQ=255)
                 tCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaa                                                                                                                                >  2:3245903/1‑148 (MQ=255)
                 tCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaa                                                                                                                                >  2:21169/1‑148 (MQ=255)
                 tCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaa                                                                                                                                >  2:21666/1‑148 (MQ=255)
                  cACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATAGAACGCAAAAATAATTCTCTAAGTAaatgaat                                                                                                                               <  2:2343681/148‑1 (MQ=255)
                    cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                             <  1:3553716/148‑1 (MQ=255)
                    cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                             >  2:3467824/1‑148 (MQ=255)
                    cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                             >  1:2691376/1‑148 (MQ=255)
                    cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                             <  1:1544572/148‑1 (MQ=255)
                    cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                             <  2:1182639/148‑1 (MQ=255)
                       ccAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAtt                                                                                                                          >  2:2818684/1‑148 (MQ=255)
                       ccAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAtt                                                                                                                          >  1:1762965/1‑148 (MQ=255)
                        cAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTg                                                                                                                         >  2:1783009/1‑148 (MQ=255)
                          ggAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCa                                                                                                                       >  2:247497/1‑148 (MQ=255)
                              aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCg                                                                                                                   <  2:1689001/148‑1 (MQ=255)
                              aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCg                                                                                                                   <  1:873086/148‑1 (MQ=255)
                               aaacaaacATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                  <  1:1958811/148‑1 (MQ=255)
                               aaacaaacATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                  >  1:2337341/1‑148 (MQ=255)
                                aacaaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGtt                                                                                                                 >  1:448634/1‑148 (MQ=255)
                                  caaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTc                                                                                                               >  2:972435/1‑148 (MQ=255)
                                        tttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCaa                                                                                                         >  2:33430/1‑148 (MQ=255)
                                          tCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAtt                                                                                                       <  2:1814723/148‑1 (MQ=255)
                                            cAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGt                                                                                                     <  2:559932/148‑1 (MQ=255)
                                             aaaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTa                                                                                                    <  2:1514827/148‑1 (MQ=255)
                                              aaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAc                                                                                                   >  2:2489275/1‑148 (MQ=255)
                                              aaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAc                                                                                                   >  1:2948976/1‑148 (MQ=255)
                                              aaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAc                                                                                                   >  1:1574999/1‑148 (MQ=255)
                                                   aaTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTaa                                                                                              <  1:1037717/148‑1 (MQ=255)
                                                    aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAt                                                                                             >  1:1064374/1‑148 (MQ=255)
                                                     tAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAtt                                                                                            >  2:1106263/1‑148 (MQ=255)
                                                      aaCTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTg                                                                                           <  2:2975076/148‑1 (MQ=255)
                                                      aaCTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTg                                                                                           <  1:281428/148‑1 (MQ=255)
                                                       aCTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGa                                                                                          <  2:1295929/148‑1 (MQ=255)
                                                         tCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAcc                                                                                        <  1:2226158/148‑1 (MQ=255)
                                                         tCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAcc                                                                                        <  2:979407/148‑1 (MQ=255)
                                                         tCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAcc                                                                                        >  1:564070/1‑148 (MQ=255)
                                                          cacaGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCa                                                                                       <  1:3245903/148‑1 (MQ=255)
                                                             aGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAcc                                                                                    >  1:1230236/1‑148 (MQ=255)
                                                              gTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAccc                                                                                   >  1:2768787/1‑148 (MQ=255)
                                                                aaTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCg                                                                                 >  1:588795/1‑148 (MQ=255)
                                                                 aTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGc                                                                                >  2:192469/1‑148 (MQ=255)
                                                                    aaCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCtta                                                                             >  2:1025434/1‑148 (MQ=255)
                                                                     aCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttat                                                                            <  1:1210290/148‑1 (MQ=255)
                                                                     aCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttat                                                                            <  2:646082/148‑1 (MQ=255)
                                                                       atcatcAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatta                                                                          <  2:3090598/148‑1 (MQ=255)
                                                                        tcatcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTaa                                                                         >  1:123788/1‑148 (MQ=255)
                                                                         catcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAAc                                                                        <  1:787845/148‑1 (MQ=255)
                                                                          atcaGGGTTATTTTTATAGTGATGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACt                                                                       <  2:2607650/148‑1 (MQ=255)
                                                                           tcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                      >  1:885195/1‑148 (MQ=255)
                                                                           tcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                      <  1:3476013/148‑1 (MQ=255)
                                                                           tcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                      <  1:1796680/148‑1 (MQ=255)
                                                                            caGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACttt                                                                     <  2:1368364/148‑1 (MQ=255)
                                                                            caGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACttt                                                                     >  2:2565265/1‑148 (MQ=255)
                                                                            caGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACttt                                                                     <  2:309212/148‑1 (MQ=255)
                                                                              gggTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCt                                                                   >  1:2072398/1‑148 (MQ=255)
                                                                               ggTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTg                                                                  >  1:801615/1‑148 (MQ=255)
                                                                               ggTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTg                                                                  >  1:1885860/1‑148 (MQ=255)
                                                                                gTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGt                                                                 <  2:1640235/148‑1 (MQ=255)
                                                                                     ttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATACTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAc                                                            <  1:38209/148‑1 (MQ=255)
                                                                                        tataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACttt                                                         >  2:2711704/1‑148 (MQ=255)
                                                                                             tGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCtt                                                    >  1:299017/1‑148 (MQ=255)
                                                                                              gAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTta                                                   <  2:1661782/148‑1 (MQ=255)
                                                                                                 gATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaa                                                >  2:743160/1‑148 (MQ=255)
                                                                                                 gATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaa                                                >  1:413886/1‑148 (MQ=255)
                                                                                                  aTAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaa                                               >  1:1596337/1‑148 (MQ=255)
                                                                                                   tAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaa                                              >  1:2899352/1‑148 (MQ=255)
                                                                                                        cTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCa                                         <  1:2359356/148‑1 (MQ=255)
                                                                                                          gatgatGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATg                                       <  1:3045498/148‑1 (MQ=255)
                                                                                                          gatgatGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATg                                       >  2:3531469/1‑148 (MQ=255)
                                                                                                             gatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaa                                    >  2:3077816/1‑148 (MQ=255)
                                                                                                               tgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaaa                                  >  1:3184602/1‑148 (MQ=255)
                                                                                                                gcgcACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAc                                 <  1:1291221/148‑1 (MQ=255)
                                                                                                                   cACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGc                              <  1:3073049/148‑1 (MQ=255)
                                                                                                                    aCCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCt                             >  2:3139284/1‑148 (MQ=255)
                                                                                                                    aCCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCt                             <  2:352126/148‑1 (MQ=255)
                                                                                                                       gTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGc                          >  2:173750/1‑148 (MQ=255)
                                                                                                                           tttCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAg                      <  1:2443107/148‑1 (MQ=255)
                                                                                                                            ttCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGa                     <  2:2000657/148‑1 (MQ=255)
                                                                                                                            ttCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGa                     <  2:714579/148‑1 (MQ=255)
                                                                                                                             tCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGAc                    <  2:1885860/148‑1 (MQ=255)
                                                                                                                                  tATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCAc               <  1:2493169/148‑1 (MQ=255)
                                                                                                                                      gAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAAt           >  1:1441796/1‑148 (MQ=255)
                                                                                                                                      gAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAAt           >  1:2586218/1‑148 (MQ=255)
                                                                                                                                        aCGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCg         >  2:1886659/1‑148 (MQ=255)
                                                                                                                                             aaaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTaa    >  2:1579874/1‑148 (MQ=255)
                                                                                                                                               aataatTCTCTAAGTAAATGAATGGATTGTATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtc  <  2:3555563/148‑1 (MQ=255)
                                                                                                                                               aataatTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtc  >  2:624123/1‑148 (MQ=255)
                                                                                                                                               aataatTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtc  <  1:327748/148‑1 (MQ=255)
                                                                                                                                               aataatTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACAGCTCGCCAAGACCGCACCAATCGGGTAAtc  >  2:2414650/1‑148 (MQ=255)
                                                                                                                                               aataatTCTCTAAGTAAATGAATGGATTACATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAtc  >  1:381775/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATC  >  CP009273/2262991‑2263281

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  >  CP009273/2262988‑2263283
                                                                                                                                                     |                                                                                                                                                  
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATA                                                                                                                                                    >  SRR19973066.650764/1‑150 (MQ=60)
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATA                                                                                                                                                    >  SRR19973066.2586723/1‑150 (MQ=60)
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATA                                                                                                                                                    >  SRR19973066.3495595/1‑150 (MQ=60)
  ATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAAT                                                                                                                                                  >  SRR19973066.354991/1‑150 (MQ=60)
  ATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAAT                                                                                                                                                  >  SRR19973066.354869/1‑150 (MQ=60)
    CACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTC                                                                                                                                                >  SRR19973066.513157/1‑150 (MQ=60)
     ACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCT                                                                                                                                               >  SRR19973066.2975858/1‑150 (MQ=60)
      CCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTC                                                                                                                                              >  SRR19973066.629484/1‑150 (MQ=60)
      CCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTC                                                                                                                                              >  SRR19973066.629186/1‑150 (MQ=60)
       CTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCT                                                                                                                                             >  SRR19973066.2888405/1‑150 (MQ=60)
         TTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAA                                                                                                                                           <  SRR19973066.2801629/150‑1 (MQ=60)
          TTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAG                                                                                                                                          <  SRR19973066.2369909/150‑1 (MQ=60)
              GACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAA                                                                                                                                      >  SRR19973066.421330/1‑150 (MQ=60)
               ACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAAT                                                                                                                                     <  SRR19973066.3543214/150‑1 (MQ=60)
               ACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAAT                                                                                                                                     <  SRR19973066.2731965/150‑1 (MQ=60)
               ACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTCAGCTAAATCCTGATGATGCGCACAGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAAT                                                                                                                                     <  SRR19973066.3521277/150‑1 (MQ=60)
                CAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATG                                                                                                                                    <  SRR19973066.1027032/150‑1 (MQ=60)
                CAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATG                                                                                                                                    <  SRR19973066.1026313/150‑1 (MQ=60)
                 AAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGA                                                                                                                                   <  SRR19973066.386753/150‑1 (MQ=60)
                     CACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGG                                                                                                                               >  SRR19973066.2709349/1‑150 (MQ=60)
                     CACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGG                                                                                                                               <  SRR19973066.421721/150‑1 (MQ=60)
                      ACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGA                                                                                                                              <  SRR19973066.1989420/150‑1 (MQ=60)
                       CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                             <  SRR19973066.1555624/150‑1 (MQ=60)
                       CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                             <  SRR19973066.3578348/150‑1 (MQ=60)
                       CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                             <  SRR19973066.3577290/150‑1 (MQ=60)
                        TACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATT                                                                                                                            >  SRR19973066.1775614/1‑150 (MQ=60)
                        TACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATT                                                                                                                            <  SRR19973066.3073738/150‑1 (MQ=60)
                              GAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGC                                                                                                                      >  SRR19973066.883159/1‑150 (MQ=60)
                              GAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGC                                                                                                                      >  SRR19973066.883146/1‑150 (MQ=60)
                                AAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGT                                                                                                                    >  SRR19973066.2353266/1‑150 (MQ=60)
                                 AAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTT                                                                                                                   >  SRR19973066.452090/1‑150 (MQ=60)
                                 AAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTT                                                                                                                   <  SRR19973066.879330/150‑1 (MQ=60)
                                  AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                  <  SRR19973066.1972622/150‑1 (MQ=60)
                                  AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                  <  SRR19973066.1972665/150‑1 (MQ=60)
                                  AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                  >  SRR19973066.589590/1‑150 (MQ=60)
                                  AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                  <  SRR19973066.158696/150‑1 (MQ=60)
                                   AACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTC                                                                                                                 >  SRR19973066.1287549/1‑150 (MQ=60)
                                   AACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTC                                                                                                                 >  SRR19973066.2628299/1‑150 (MQ=60)
                                               CAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAC                                                                                                     >  SRR19973066.2968543/1‑150 (MQ=60)
                                               CAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAC                                                                                                     >  SRR19973066.1586350/1‑150 (MQ=60)
                                                   ACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTA                                                                                                 <  SRR19973066.2562214/150‑1 (MQ=60)
                                                   ACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTA                                                                                                 <  SRR19973066.38541/150‑1 (MQ=60)
                                                     AAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAT                                                                                               >  SRR19973066.1058417/1‑150 (MQ=60)
                                                     AAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAT                                                                                               >  SRR19973066.1072176/1‑150 (MQ=60)
                                                      AATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATT                                                                                              >  SRR19973066.550364/1‑150 (MQ=60)
                                                      AATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATT                                                                                              <  SRR19973066.1045353/150‑1 (MQ=60)
                                                      AATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATT                                                                                              <  SRR19973066.674519/150‑1 (MQ=60)
                                                      AATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATT                                                                                              <  SRR19973066.674563/150‑1 (MQ=60)
                                                        TAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGA                                                                                            >  SRR19973066.3224014/1‑150 (MQ=60)
                                                        TAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGA                                                                                            <  SRR19973066.3212926/150‑1 (MQ=60)
                                                         AACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAC                                                                                           <  SRR19973066.283475/150‑1 (MQ=60)
                                                          ACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACC                                                                                          >  SRR19973066.568081/1‑150 (MQ=60)
                                                          ACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACC                                                                                          <  SRR19973066.1276934/150‑1 (MQ=60)
                                                            TCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAA                                                                                        <  SRR19973066.2241544/150‑1 (MQ=60)
                                                             CACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAC                                                                                       <  SRR19973066.3267301/150‑1 (MQ=60)
                                                              ACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACC                                                                                      >  SRR19973066.1238983/1‑150 (MQ=60)
                                                              ACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACC                                                                                      >  SRR19973066.1238975/1‑150 (MQ=60)
                                                               CAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCC                                                                                     >  SRR19973066.2787434/1‑150 (MQ=60)
                                                                 GTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCG                                                                                   >  SRR19973066.592895/1‑150 (MQ=60)
                                                                  TAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGC                                                                                  >  SRR19973066.800526/1‑150 (MQ=60)
                                                                   AATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCT                                                                                 >  SRR19973066.1740842/1‑150 (MQ=60)
                                                                    ATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTT                                                                                <  SRR19973066.2421529/150‑1 (MQ=60)
                                                                      TAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTAT                                                                              >  SRR19973066.3269017/1‑150 (MQ=60)
                                                                        ACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTA                                                                            <  SRR19973066.1218757/150‑1 (MQ=60)
                                                                        ACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTA                                                                            <  SRR19973066.1219141/150‑1 (MQ=60)
                                                                         CATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAA                                                                           >  SRR19973066.125029/1‑150 (MQ=60)
                                                                            CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                        <  SRR19973066.793651/150‑1 (MQ=60)
                                                                            CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                        >  SRR19973066.891488/1‑150 (MQ=60)
                                                                            CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                        >  SRR19973066.891496/1‑150 (MQ=60)
                                                                              TCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTC                                                                      <  SRR19973066.3499218/150‑1 (MQ=60)
                                                                              TCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTC                                                                      <  SRR19973066.2889699/150‑1 (MQ=60)
                                                                              TCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTC                                                                      <  SRR19973066.2493329/150‑1 (MQ=60)
                                                                              TCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTC                                                                      <  SRR19973066.1809456/150‑1 (MQ=60)
                                                                               CAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCT                                                                     >  SRR19973066.578548/1‑150 (MQ=60)
                                                                               CAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCT                                                                     >  SRR19973066.2086735/1‑150 (MQ=60)
                                                                                AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                    >  SRR19973066.1899340/1‑150 (MQ=60)
                                                                                AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                    >  SRR19973066.1899306/1‑150 (MQ=60)
                                                                                AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                    >  SRR19973066.807508/1‑150 (MQ=60)
                                                                                 GGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGT                                                                   >  SRR19973066.2682733/1‑150 (MQ=60)
                                                                                  GGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTA                                                                  >  SRR19973066.2829727/1‑150 (MQ=60)
                                                                                  GGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTA                                                                  >  SRR19973066.3101395/1‑150 (MQ=60)
                                                                                    TTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATC                                                                <  SRR19973066.1167009/150‑1 (MQ=60)
                                                                                        TTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATACTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTT                                                            <  SRR19973066.38716/150‑1 (MQ=60)
                                                                                            ATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTC                                                        <  SRR19973066.1540705/150‑1 (MQ=60)
                                                                                            ATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTC                                                        >  SRR19973066.2931464/1‑150 (MQ=60)
                                                                                             TAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCT                                                       <  SRR19973066.2863686/150‑1 (MQ=60)
                                                                                              AGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTT                                                      >  SRR19973066.301128/1‑150 (MQ=60)
                                                                                              AGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTT                                                      <  SRR19973066.1087297/150‑1 (MQ=60)
                                                                                                TGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGNATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTAT                                                    <  SRR19973066.1822018/150‑1 (MQ=60)
                                                                                                  AGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAA                                                  >  SRR19973066.2936563/1‑150 (MQ=60)
                                                                                                  AGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAA                                                  >  SRR19973066.417119/1‑150 (MQ=60)
                                                                                                   GGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAA                                                 >  SRR19973066.1607875/1‑150 (MQ=60)
                                                                                                   GGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAA                                                 >  SRR19973066.1607899/1‑150 (MQ=60)
                                                                                                    GATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAA                                                >  SRR19973066.2918729/1‑150 (MQ=60)
                                                                                                     ATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAA                                               >  SRR19973066.2288142/1‑150 (MQ=60)
                                                                                                          CCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCAT                                          >  SRR19973066.510215/1‑150 (MQ=60)
                                                                                                           CTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATG                                         <  SRR19973066.2375353/150‑1 (MQ=60)
                                                                                                            TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                        >  SRR19973066.1493568/1‑150 (MQ=60)
                                                                                                             GATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTA                                       <  SRR19973066.3063132/150‑1 (MQ=60)
                                                                                                             GATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTA                                       <  SRR19973066.3065852/150‑1 (MQ=60)
                                                                                                                GATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAA                                    >  SRR19973066.3205787/1‑150 (MQ=60)
                                                                                                                   GCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCG                                 <  SRR19973066.1300567/150‑1 (MQ=60)
                                                                                                                      CACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTC                              <  SRR19973066.3093588/150‑1 (MQ=60)
                                                                                                                         CGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACTGCTCGCC                           >  SRR19973066.2388311/1‑150 (MQ=60)
                                                                                                                          GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCA                          >  SRR19973066.2209203/1‑150 (MQ=60)
                                                                                                                              TTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGAC                      <  SRR19973066.2459750/150‑1 (MQ=60)
                                                                                                                               TTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACC                     >  SRR19973066.1684629/1‑150 (MQ=60)
                                                                                                                                     TATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCA               <  SRR19973066.2510169/150‑1 (MQ=60)
                                                                                                                                       TCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAAT             >  SRR19973066.2603743/1‑150 (MQ=60)
                                                                                                                                       TCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAAT             >  SRR19973066.1452058/1‑150 (MQ=60)
                                                                                                                                        CGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATC            >  SRR19973066.1804881/1‑150 (MQ=60)
                                                                                                                                         GAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCG           <  SRR19973066.3490997/150‑1 (MQ=60)
                                                                                                                                              CAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAA      <  SRR19973066.810240/150‑1 (MQ=60)
                                                                                                                                               AAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAT     >  SRR19973066.3019041/1‑150 (MQ=60)
                                                                                                                                               AAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACAGCTCGCCAAGACCGCACCAATCGGGTAAT     >  SRR19973066.2158190/1‑150 (MQ=60)
                                                                                                                                                AAAATAATTCTCTAAGTAAATGAATGGATTACATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATC    >  SRR19973066.384789/1‑150 (MQ=60)
                                                                                                                                                AAAATAATTCTCTAAGTAAATGAATGGATTACATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATC    >  SRR19973066.1347204/1‑150 (MQ=60)
                                                                                                                                                AAAATAATTCTCTAAGTAAATGAATGGATTACATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAATTTTTCTTATAAAAAATCATGTAAAACAGCTCGCCAAGACCGCACCAATCGGGTAATC    >  SRR19973066.385043/1‑150 (MQ=60)
                                                                                                                                                 AAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTTTAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCT   >  SRR19973066.3143077/1‑150 (MQ=60)
                                                                                                                                                  AATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  <  SRR19973066.330243/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
TAATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  >  CP009273/2262988‑2263283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑