Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,263,137 C→A 100% intergenic (+91/‑321) lpxT → / → mepS lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,263,1370CA98.2% 711.1 / 3.4 222intergenic (+91/‑321)lpxT/mepSlipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (3/1);  new base A (130/88);  total (133/89)
Fisher's exact test for biased strand distribution p-value = 6.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

CACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAA  >  CP009273/2262992‑2263279
                                                                                                                                                 |                                                                                                                                              
cACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAaataat                                                                                                                                              >  2:1351195/1‑148 (MQ=255)
 aCCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCTCACCGTGCTTTCATCTATCGAACGCAAAAATAAtt                                                                                                                                             >  2:2956245/1‑148 (MQ=255)
   cTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtct                                                                                                                                           <  1:195115/148‑1 (MQ=255)
    ttttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtctc                                                                                                                                          >  2:2816398/1‑148 (MQ=255)
    ttttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtctc                                                                                                                                          >  2:455404/1‑148 (MQ=255)
     tttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTctct                                                                                                                                         >  1:1902512/1‑148 (MQ=255)
      ttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTa                                                                                                                                        <  2:3397576/148‑1 (MQ=255)
      ttgtGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTa                                                                                                                                        <  2:3361962/145‑1 (MQ=255)
        ttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAg                                                                                                                                      <  2:563171/148‑1 (MQ=255)
         tGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGt                                                                                                                                     >  2:695446/1‑148 (MQ=255)
            cAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTCAGTaaa                                                                                                                                  >  2:2204526/1‑148 (MQ=255)
            cAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTaaa                                                                                                                                  <  2:2689701/148‑1 (MQ=255)
            cAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTaaa                                                                                                                                  <  2:3077969/148‑1 (MQ=255)
            cAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTaaa                                                                                                                                  >  2:1384759/1‑148 (MQ=255)
                tCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaa                                                                                                                              >  2:422539/1‑148 (MQ=255)
                tCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaa                                                                                                                              <  2:448982/148‑1 (MQ=255)
                tCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaa                                                                                                                              <  2:2102455/148‑1 (MQ=255)
                  aCTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaatg                                                                                                                            >  1:655963/1‑148 (MQ=255)
                   cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                           >  2:872001/1‑148 (MQ=255)
                   cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATgg                                                                                                                           <  2:2709664/148‑1 (MQ=255)
                       cAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTg                                                                                                                       >  1:2387209/1‑148 (MQ=255)
                       cAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTg                                                                                                                       >  1:1801182/1‑148 (MQ=255)
                            aaaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGc                                                                                                                  >  1:2700604/1‑148 (MQ=255)
                             aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCg                                                                                                                 <  1:2798938/148‑1 (MQ=255)
                             aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCg                                                                                                                 >  1:1071989/1‑148 (MQ=255)
                             aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCg                                                                                                                 >  2:2181069/1‑148 (MQ=255)
                              aaacaaacATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                <  1:3818731/148‑1 (MQ=255)
                                 caaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTc                                                                                                             >  2:1647615/1‑148 (MQ=255)
                                 caaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTc                                                                                                             >  2:598141/1‑148 (MQ=255)
                                  aaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCa                                                                                                            <  1:555392/148‑1 (MQ=255)
                                   aacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCAc                                                                                                           >  2:3658911/1‑148 (MQ=255)
                                    acaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACt                                                                                                          >  1:702845/1‑148 (MQ=255)
                                        ttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAt                                                                                                      <  1:3714765/148‑1 (MQ=255)
                                        ttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAt                                                                                                      >  2:3788110/1‑148 (MQ=255)
                                            aaaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTa                                                                                                  >  1:1586562/1‑148 (MQ=255)
                                            aaaaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTa                                                                                                  >  1:229186/1‑148 (MQ=255)
                                              aaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACt                                                                                                >  1:3262760/1‑148 (MQ=255)
                                              aaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACt                                                                                                <  1:346713/148‑1 (MQ=255)
                                               aCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACtt                                                                                               >  2:3214812/1‑148 (MQ=255)
                                                   aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAt                                                                                           <  1:3296098/148‑1 (MQ=255)
                                                   aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAt                                                                                           <  1:1379063/148‑1 (MQ=255)
                                                   aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAt                                                                                           <  1:1715253/148‑1 (MQ=255)
                                                    tAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAtt                                                                                          >  2:1982270/1‑148 (MQ=255)
                                                      aCTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGa                                                                                        >  2:1192795/1‑148 (MQ=255)
                                                       cTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAc                                                                                       >  2:410994/1‑148 (MQ=255)
                                                        tCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAcc                                                                                      >  1:7501/1‑148 (MQ=255)
                                                            aGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAcc                                                                                  >  2:3759029/1‑148 (MQ=255)
                                                              tAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAcccc                                                                                >  1:3092980/1‑148 (MQ=255)
                                                                aTTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGc                                                                              <  1:455404/148‑1 (MQ=255)
                                                                     catcatCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatt                                                                         >  2:2767376/1‑148 (MQ=255)
                                                                     catcatCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatt                                                                         >  2:2285249/1‑148 (MQ=255)
                                                                     catcatCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAATTTAATTGACCAACCCCGCttatt                                                                         >  2:3465250/1‑148 (MQ=255)
                                                                      atcatcAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatta                                                                        <  1:3031385/148‑1 (MQ=255)
                                                                      atcatcAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatta                                                                        <  2:771293/148‑1 (MQ=255)
                                                                      atcatcAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatta                                                                        <  2:1760628/148‑1 (MQ=255)
                                                                          tcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                    >  1:2900388/1‑148 (MQ=255)
                                                                          tcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                    >  1:2130240/1‑148 (MQ=255)
                                                                            aGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAAc                                                                      <  1:3234929/144‑1 (MQ=255)
                                                                             gggTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCt                                                                 >  1:3064273/1‑148 (MQ=255)
                                                                             gggTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCt                                                                 <  1:2439998/148‑1 (MQ=255)
                                                                               gTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGt                                                               >  2:3325785/1‑148 (MQ=255)
                                                                                 tATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAt                                                             >  1:2010247/1‑148 (MQ=255)
                                                                                 tATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAt                                                             >  2:167610/1‑148 (MQ=255)
                                                                                 tATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAt                                                             <  2:3413844/148‑1 (MQ=255)
                                                                                    ttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTTACCAACCCCGCTTATTAACTTTCTTTATCAc                                                          >  1:1740014/1‑148 (MQ=255)
                                                                                    ttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAc                                                          >  1:1640407/1‑148 (MQ=255)
                                                                                    ttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAc                                                          >  2:2863160/1‑148 (MQ=255)
                                                                                     tttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACt                                                         <  1:422539/148‑1 (MQ=255)
                                                                                     tttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACt                                                         <  2:1051852/148‑1 (MQ=255)
                                                                                         taGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACttttt                                                     <  1:1516065/148‑1 (MQ=255)
                                                                                           gagAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAAAGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAAATGACCAACACCGCTTATTAACTTTCTGTATCACatttaat                                                   >  2:2093729/3‑145 (MQ=255)
                                                                                           gTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCt                                                   <  2:1081881/148‑1 (MQ=255)
                                                                                              aGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTtat                                                >  2:3305496/1‑148 (MQ=255)
                                                                                                gATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaa                                              >  2:2337833/1‑148 (MQ=255)
                                                                                                   aaTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaa                                           >  1:48057/1‑148 (MQ=255)
                                                                                                   aaTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaa                                           >  1:3614997/1‑148 (MQ=255)
                                                                                                    aTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaaa                                          >  1:908474/1‑148 (MQ=255)
                                                                                                     tCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAAt                                         >  2:3504461/1‑148 (MQ=255)
                                                                                                      ccTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATc                                        >  1:11473/1‑148 (MQ=255)
                                                                                                            gatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaa                                  <  2:2657092/148‑1 (MQ=255)
                                                                                                             atgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaa                                 <  2:729350/148‑1 (MQ=255)
                                                                                                             atgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaa                                 >  2:1809676/1‑148 (MQ=255)
                                                                                                             atgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaa                                 >  1:2984175/1‑148 (MQ=255)
                                                                                                                   aCCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCt                           >  1:1507412/1‑148 (MQ=255)
                                                                                                                      gTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGc                        <  1:3076562/148‑1 (MQ=255)
                                                                                                                        gCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCa                      <  1:1239788/148‑1 (MQ=255)
                                                                                                                        gCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCa                      >  1:1615943/1‑148 (MQ=255)
                                                                                                                         cTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCaa                     >  1:1543239/1‑148 (MQ=255)
                                                                                                                              aTCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCg                <  2:2958973/148‑1 (MQ=255)
                                                                                                                               tCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGc               >  1:3178830/1‑148 (MQ=255)
                                                                                                                                   tCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCa           >  2:3445129/1‑148 (MQ=255)
                                                                                                                                    cGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCaa          >  1:607303/1‑148 (MQ=255)
                                                                                                                                    cGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCaa          <  2:3574646/148‑1 (MQ=255)
                                                                                                                                    cGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCaa          <  2:1507412/148‑1 (MQ=255)
                                                                                                                                     gAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTACCTTTCTTTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAAt         <  1:1982270/148‑1 (MQ=255)
                                                                                                                                        cGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCgg      >  1:3341082/1‑148 (MQ=255)
                                                                                                                                        cGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCgg      >  2:3634065/1‑148 (MQ=255)
                                                                                                                                        cGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCgg      >  2:3631911/1‑148 (MQ=255)
                                                                                                                                           aaaaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTa   <  1:410994/148‑1 (MQ=255)
                                                                                                                                            aaaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTaa  >  1:1305735/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAA  >  CP009273/2262992‑2263279

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  >  CP009273/2262990‑2263283
                                                                                                                                                   |                                                                                                                                                  
ATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAAT                                                                                                                                                  <  SRR19973069.1643064/150‑1 (MQ=60)
 TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATT                                                                                                                                                 <  SRR19973069.3654542/150‑1 (MQ=60)
 TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATT                                                                                                                                                 <  SRR19973069.1573410/150‑1 (MQ=60)
 TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATT                                                                                                                                                 <  SRR19973069.908668/150‑1 (MQ=60)
     CTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCT                                                                                                                                             >  SRR19973069.1915661/1‑150 (MQ=60)
     CTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCT                                                                                                                                             <  SRR19973069.196697/150‑1 (MQ=60)
           TGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAA                                                                                                                                       >  SRR19973069.1976208/1‑150 (MQ=60)
               AAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGA                                                                                                                                   <  SRR19973069.411314/150‑1 (MQ=60)
                 ATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAAT                                                                                                                                 >  SRR19973069.2448803/1‑150 (MQ=60)
                  TCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATG                                                                                                                                >  SRR19973069.660370/1‑150 (MQ=60)
                   CACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTCACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGT                                                                                                                               >  SRR19973069.679211/1‑150 (MQ=60)
                    ACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGA                                                                                                                              >  SRR19973069.3084817/1‑150 (MQ=60)
                     CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                             <  SRR19973069.543826/150‑1 (MQ=60)
                       ACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTG                                                                                                                           >  SRR19973069.1813862/1‑150 (MQ=60)
                       ACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTG                                                                                                                           >  SRR19973069.2403393/1‑150 (MQ=60)
                          AGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCAT                                                                                                                        >  SRR19973069.2674902/1‑150 (MQ=60)
                            GAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGC                                                                                                                      >  SRR19973069.2718701/1‑150 (MQ=60)
                             AAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTTCATGCG                                                                                                                     >  SRR19973069.1923116/1‑150 (MQ=60)
                             AAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCG                                                                                                                     >  SRR19973069.3823014/1‑150 (MQ=60)
                             AAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCG                                                                                                                     >  SRR19973069.1079467/1‑150 (MQ=60)
                              AAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGT                                                                                                                    <  SRR19973069.2001538/150‑1 (MQ=60)
                               AAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTT                                                                                                                   <  SRR19973069.2817527/150‑1 (MQ=60)
                               AAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTT                                                                                                                   <  SRR19973069.2817546/150‑1 (MQ=60)
                                AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                  <  SRR19973069.3843442/150‑1 (MQ=60)
                                 AACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTC                                                                                                                 <  SRR19973069.1380809/150‑1 (MQ=60)
                                 AACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTAAAGTGAGTATAATACTGATGATGCGCACCGAGCTTTCATCTATCGAAAGAAAAAAAAATACTCTAAGTAAATTAATGGATAGCTTGCGTTTC                                                                                                                 >  SRR19973069.3113655/1‑150 (MQ=60)
                                  ACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCA                                                                                                                >  SRR19973069.707343/1‑150 (MQ=60)
                                   CAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCAC                                                                                                               <  SRR19973069.3212586/150‑1 (MQ=60)
                                    AAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACT                                                                                                              <  SRR19973069.559264/150‑1 (MQ=60)
                                    AAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACT                                                                                                              <  SRR19973069.559325/150‑1 (MQ=60)
                                    AAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACT                                                                                                              >  SRR19973069.707790/1‑150 (MQ=60)
                                     AACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTC                                                                                                             <  SRR19973069.631786/150‑1 (MQ=60)
                                          TTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTG                                                                                                        <  SRR19973069.3739023/150‑1 (MQ=60)
                                          TTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTG                                                                                                        <  SRR19973069.3739073/150‑1 (MQ=60)
                                            CCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTA                                                                                                      >  SRR19973069.1597308/1‑150 (MQ=60)
                                            CCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTA                                                                                                      >  SRR19973069.230944/1‑150 (MQ=60)
                                              AAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACT                                                                                                    >  SRR19973069.3284135/1‑150 (MQ=60)
                                              AAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACT                                                                                                    >  SRR19973069.474913/1‑150 (MQ=60)
                                              AAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACT                                                                                                    <  SRR19973069.730651/150‑1 (MQ=60)
                                                AACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTT                                                                                                  <  SRR19973069.349183/150‑1 (MQ=60)
                                                AACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTT                                                                                                  <  SRR19973069.313443/150‑1 (MQ=60)
                                                AACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTT                                                                                                  <  SRR19973069.1566145/150‑1 (MQ=60)
                                                    AATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCAATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATT                                                                                              >  SRR19973069.119283/1‑150 (MQ=60)
                                                     ATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTG                                                                                             <  SRR19973069.3300640/150‑1 (MQ=60)
                                                     ATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTG                                                                                             <  SRR19973069.1727266/150‑1 (MQ=60)
                                                     ATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTG                                                                                             <  SRR19973069.1727307/150‑1 (MQ=60)
                                                     ATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTG                                                                                             <  SRR19973069.3317678/150‑1 (MQ=60)
                                                     ATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTG                                                                                             <  SRR19973069.3319059/150‑1 (MQ=60)
                                                     ATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTG                                                                                             <  SRR19973069.1388677/150‑1 (MQ=60)
                                                       AACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAC                                                                                           >  SRR19973069.2327318/1‑150 (MQ=60)
                                                        ACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACC                                                                                          >  SRR19973069.7618/1‑150 (MQ=60)
                                                              AGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCC                                                                                    >  SRR19973069.3113362/1‑150 (MQ=60)
                                                              AGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCC                                                                                    >  SRR19973069.3016334/1‑150 (MQ=60)
                                                                 AATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCT                                                                                 >  SRR19973069.2780629/1‑150 (MQ=60)
                                                                  ATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTT                                                                                <  SRR19973069.458744/150‑1 (MQ=60)
                                                                    TAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTAT                                                                              >  SRR19973069.1525220/1‑150 (MQ=60)
                                                                     AACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATT                                                                             <  SRR19973069.3650239/150‑1 (MQ=60)
                                                                     AACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATT                                                                             <  SRR19973069.1442469/150‑1 (MQ=60)
                                                                      ACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTA                                                                            >  SRR19973069.1788858/1‑150 (MQ=60)
                                                                        ATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAAC                                                                          <  SRR19973069.3051488/150‑1 (MQ=60)
                                                                         TCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACT                                                                         >  SRR19973069.1509709/1‑150 (MQ=60)
                                                                          CTTCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                        <  SRR19973069.3256090/150‑1 (MQ=60)
                                                                          CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                        >  SRR19973069.999474/1‑150 (MQ=60)
                                                                          CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                        >  SRR19973069.2144894/1‑150 (MQ=60)
                                                                          CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                        >  SRR19973069.2919540/1‑150 (MQ=60)
                                                                          CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                        >  SRR19973069.1607251/1‑150 (MQ=60)
                                                                             CAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCT                                                                     >  SRR19973069.3084538/1‑150 (MQ=60)
                                                                             CAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGAGTTTCACTCAATTGTACTTTAATTGACCAACACCGCTTATTAACTTTCT                                                                     >  SRR19973069.3083834/1‑150 (MQ=60)
                                                                               GGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGT                                                                   >  SRR19973069.1875535/1‑150 (MQ=60)
                                                                               GGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGT                                                                   <  SRR19973069.2456436/150‑1 (MQ=60)
                                                                                 GTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAT                                                                 >  SRR19973069.2024228/1‑150 (MQ=60)
                                                                                    ATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTTACCAACCCCGCTTATTAACTTTCTTTATCAC                                                              >  SRR19973069.1752220/1‑150 (MQ=60)
                                                                                    ATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAC                                                              >  SRR19973069.1649305/1‑150 (MQ=60)
                                                                                    ATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAC                                                              >  SRR19973069.1651676/1‑150 (MQ=60)
                                                                                    ATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAC                                                              <  SRR19973069.3453340/150‑1 (MQ=60)
                                                                                       TTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTT                                                           <  SRR19973069.427214/150‑1 (MQ=60)
                                                                                       TTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTT                                                           <  SRR19973069.425640/150‑1 (MQ=60)
                                                                                         TATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTT                                                         >  SRR19973069.1654361/1‑150 (MQ=60)
                                                                                           TAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCT                                                       <  SRR19973069.1526538/150‑1 (MQ=60)
                                                                                           TAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCT                                                       <  SRR19973069.1526532/150‑1 (MQ=60)
                                                                                                   ATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAA                                               >  SRR19973069.48661/1‑150 (MQ=60)
                                                                                                   ATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAA                                               >  SRR19973069.3638577/1‑150 (MQ=60)
                                                                                                   ATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAA                                               >  SRR19973069.3638606/1‑150 (MQ=60)
                                                                                                    TAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAA                                              >  SRR19973069.477027/1‑150 (MQ=60)
                                                                                                    TAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAA                                              >  SRR19973069.914701/1‑150 (MQ=60)
                                                                                                    TAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAA                                              >  SRR19973069.913448/1‑150 (MQ=60)
                                                                                                      ATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATC                                            >  SRR19973069.11635/1‑150 (MQ=60)
                                                                                                       TCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCA                                           <  SRR19973069.1121857/150‑1 (MQ=60)
                                                                                                         CTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATG                                         >  SRR19973069.3341174/1‑150 (MQ=60)
                                                                                                          TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                        >  SRR19973069.3281120/1‑150 (MQ=60)
                                                                                                           GATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTA                                       <  SRR19973069.2444556/150‑1 (MQ=60)
                                                                                                             TGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAA                                     >  SRR19973069.3003996/1‑150 (MQ=60)
                                                                                                             TGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAA                                     >  SRR19973069.3003714/1‑150 (MQ=60)
                                                                                                             TGATGCGCACCGTGCATTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTAACACAAATGTAATTTAATTTACCAACACCGCGTAGTAACTTTCAGTATCACTATTTCATATAATAAATCATCTAAA                                     >  SRR19973069.3003949/1‑150 (MQ=60)
                                                                                                               ATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAAAAATGTAAAAC                                   >  SRR19973069.31207/1‑150 (MQ=60)
                                                                                                                   GCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCT                               >  SRR19973069.1517846/1‑150 (MQ=60)
                                                                                                                        GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCA                          <  SRR19973069.3096871/150‑1 (MQ=60)
                                                                                                                        GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCA                          >  SRR19973069.1626850/1‑150 (MQ=60)
                                                                                                                        GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACATTCTGTATCACTTTTTCTTATAAAAAATCATTTAAAACCGCTCGCCA                          >  SRR19973069.1626821/1‑150 (MQ=60)
                                                                                                                         TGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAA                         >  SRR19973069.1553810/1‑150 (MQ=60)
                                                                                                                          GCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAG                        <  SRR19973069.1248220/150‑1 (MQ=60)
                                                                                                                            TTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATCAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGAC                      <  SRR19973069.1800712/150‑1 (MQ=60)
                                                                                                                               CATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGC                   >  SRR19973069.3199508/1‑150 (MQ=60)
                                                                                                                                    ATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAA              >  SRR19973069.611381/1‑150 (MQ=60)
                                                                                                                                       TAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCTTGCGTTTCACTCACTTGTACTTTAATTGACCAACCCCGCTTATTACCTTTCTTTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCG           <  SRR19973069.1996058/150‑1 (MQ=60)
                                                                                                                                       GAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCG           <  SRR19973069.1996065/150‑1 (MQ=60)
                                                                                                                                        AACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGG          >  SRR19973069.3362954/1‑150 (MQ=60)
                                                                                                                                        AACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACAGCTCGCCAAGACCGCACCAATCGG          >  SRR19973069.2061072/1‑150 (MQ=60)
                                                                                                                                           GCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTA       <  SRR19973069.2164229/150‑1 (MQ=60)
                                                                                                                                            CAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAA      >  SRR19973069.1314620/1‑150 (MQ=60)
                                                                                                                                            CAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAA      >  SRR19973069.1314552/1‑150 (MQ=60)
                                                                                                                                             AAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAT     <  SRR19973069.414633/150‑1 (MQ=60)
                                                                                                                                             AAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAT     <  SRR19973069.413999/150‑1 (MQ=60)
                                                                                                                                                AATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACAAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAACGCTCGCCAAGACCGCACCAAAAGGGTAATATC  >  SRR19973069.2355483/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                  
ATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  >  CP009273/2262990‑2263283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑