Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,263,137 C→A 100% intergenic (+91/‑321) lpxT → / → mepS lipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,263,1370CA98.2% 693.0 / 2.9 216intergenic (+91/‑321)lpxT/mepSlipid A 1‑diphosphate synthase; undecaprenyl pyrophosphate:lipid A 1‑phosphate phosphotransferase/murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/4);  new base A (116/96);  total (116/100)
Fisher's exact test for biased strand distribution p-value = 4.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  >  CP009273/2262991‑2263283
                                                                                                                                                  |                                                                                                                                                  
 cACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtc                                                                                                                                                >  2:1174373/1‑150 (MQ=255)
 cACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtc                                                                                                                                                >  1:853373/1‑150 (MQ=255)
  aCCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATtct                                                                                                                                               >  2:4655430/1‑150 (MQ=255)
       ttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAg                                                                                                                                          <  1:1997093/150‑1 (MQ=255)
       ttttGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAg                                                                                                                                          <  2:450702/150‑1 (MQ=255)
             cAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatg                                                                                                                                    <  1:5029037/150‑1 (MQ=255)
                 tCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAaatgaatg                                                                                                                                <  1:2582781/150‑1 (MQ=255)
                   aCTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGa                                                                                                                              >  1:2254199/1‑150 (MQ=255)
                    cTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAt                                                                                                                             >  1:1268769/1‑150 (MQ=255)
                     tACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGagt                                                                                                                            >  2:4851570/1‑148 (MQ=255)
                      aCCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTg                                                                                                                           >  1:3694761/1‑150 (MQ=255)
                            aaaaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCg                                                                                                                     >  2:5133025/1‑150 (MQ=255)
                             aaaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                    >  2:659247/1‑150 (MQ=255)
                             aaaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGt                                                                                                                    >  2:659358/1‑150 (MQ=255)
                              aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGtt                                                                                                                   >  1:2776058/1‑150 (MQ=255)
                              aaaaCAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGtt                                                                                                                   <  1:4672067/150‑1 (MQ=255)
                               aaacaaacATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGttt                                                                                                                  <  1:1159846/150‑1 (MQ=255)
                                 acaaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCa                                                                                                                >  2:1585370/1‑150 (MQ=255)
                                  caaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCAc                                                                                                               <  2:3205029/150‑1 (MQ=255)
                                   aaacaTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACt                                                                                                              <  1:147250/150‑1 (MQ=255)
                                      caTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCaa                                                                                                           <  2:4714817/150‑1 (MQ=255)
                                       aTTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAt                                                                                                          <  1:5005940/150‑1 (MQ=255)
                                         ttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTg                                                                                                        <  1:3693669/150‑1 (MQ=255)
                                         ttCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTg                                                                                                        <  1:3212224/150‑1 (MQ=255)
                                          tCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGt                                                                                                       >  1:4841824/1‑150 (MQ=255)
                                          tCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGt                                                                                                       >  1:2044971/1‑150 (MQ=255)
                                           ccAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTa                                                                                                      <  1:3017073/150‑1 (MQ=255)
                                           ccAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTa                                                                                                      >  1:4074864/1‑150 (MQ=255)
                                            cAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTaa                                                                                                     >  2:1510069/1‑149 (MQ=255)
                                            cAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAc                                                                                                     >  1:3566889/1‑150 (MQ=255)
                                            cAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAc                                                                                                     >  2:1893257/1‑150 (MQ=255)
                                               aaCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACttt                                                                                                  >  2:839553/1‑150 (MQ=255)
                                                aCAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTa                                                                                                 <  2:1282937/150‑1 (MQ=255)
                                                 cAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTaa                                                                                                >  1:1804987/1‑150 (MQ=255)
                                                    aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTCAATGAATGGATTGCATGAGTTTCACTCAATTGTACTTTAAttt                                                                                             >  2:2642031/1‑149 (MQ=255)
                                                    aTAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTg                                                                                             >  2:3877117/1‑150 (MQ=255)
                                                         tCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCaa                                                                                        <  2:4941885/150‑1 (MQ=255)
                                                               tAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGc                                                                                  >  1:4538301/1‑150 (MQ=255)
                                                                   tAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttat                                                                              <  1:1951878/150‑1 (MQ=255)
                                                                    aaCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatt                                                                             <  1:563439/150‑1 (MQ=255)
                                                                    aaCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatt                                                                             >  1:3090239/1‑150 (MQ=255)
                                                                    aaCATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATACTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCttatt                                                                             >  2:2548936/1‑150 (MQ=255)
                                                                         catcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                        >  2:4992206/1‑150 (MQ=255)
                                                                         catcaGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACtt                                                                        >  2:226662/1‑150 (MQ=255)
                                                                             aGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCAGTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTg                                                                    <  1:3023037/150‑1 (MQ=255)
                                                                             aGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTg                                                                    <  1:2972527/150‑1 (MQ=255)
                                                                             aGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTg                                                                    >  1:5128642/1‑150 (MQ=255)
                                                                                gTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAt                                                                 <  1:1705026/150‑1 (MQ=255)
                                                                                gTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAt                                                                 >  2:2059028/1‑150 (MQ=255)
                                                                                 ttATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATc                                                                <  2:1015174/150‑1 (MQ=255)
                                                                                 ttATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATc                                                                <  1:3285567/150‑1 (MQ=255)
                                                                                  tATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCa                                                               >  1:2038605/1‑150 (MQ=255)
                                                                                  tATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCa                                                               <  2:2869216/150‑1 (MQ=255)
                                                                                   aTTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAc                                                              >  1:4266028/1‑150 (MQ=255)
                                                                                    tttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACt                                                             >  2:3496501/1‑150 (MQ=255)
                                                                                     ttttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACtt                                                            <  1:1019744/150‑1 (MQ=255)
                                                                                      tttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACttt                                                           <  1:1220247/150‑1 (MQ=255)
                                                                                       ttATAGTGAGGATAATCCTGATGGTGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACtttt                                                          <  2:4018103/150‑1 (MQ=255)
                                                                                       ttATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACtttt                                                          >  2:2192295/1‑150 (MQ=255)
                                                                                        tataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACttttt                                                         >  2:2764300/1‑150 (MQ=255)
                                                                                        tataGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACttttt                                                         >  1:2826474/1‑150 (MQ=255)
                                                                                          taGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCt                                                       >  1:849170/1‑150 (MQ=255)
                                                                                           aGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCtt                                                      >  1:2957208/1‑150 (MQ=255)
                                                                                             tGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTtat                                                    <  1:2548225/150‑1 (MQ=255)
                                                                                              gAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTtata                                                   <  1:2348569/150‑1 (MQ=255)
                                                                                              gAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGAAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTtata                                                   <  2:4634007/150‑1 (MQ=255)
                                                                                               aGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaa                                                  >  1:3888460/1‑150 (MQ=255)
                                                                                                ggATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaa                                                 >  1:3572455/1‑150 (MQ=255)
                                                                                                 gATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaa                                                >  1:1133771/1‑150 (MQ=255)
                                                                                                 gATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaa                                                >  1:4582568/1‑150 (MQ=255)
                                                                                                  aTAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTCTaaaac                                               >  2:3371821/1‑149 (MQ=255)
                                                                                                  aTAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaa                                               >  2:1466943/1‑150 (MQ=255)
                                                                                                  aTAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaa                                               >  2:4019335/1‑150 (MQ=255)
                                                                                                   tAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATaaaaaa                                              >  2:715407/1‑150 (MQ=255)
                                                                                                    aaTCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAAt                                             <  1:673676/150‑1 (MQ=255)
                                                                                                       ccTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCAt                                          >  1:3834597/1‑150 (MQ=255)
                                                                                                        cTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATg                                         >  1:4003565/1‑150 (MQ=255)
                                                                                                          gatgatGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTa                                       >  2:2191597/1‑150 (MQ=255)
                                                                                                           atgatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaa                                      <  1:4413343/150‑1 (MQ=255)
                                                                                                           atgatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaa                                      >  1:4908764/1‑150 (MQ=255)
                                                                                                            tgatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaa                                     >  2:3530327/1‑150 (MQ=255)
                                                                                                             gatgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTaaaa                                    <  2:4104856/150‑1 (MQ=255)
                                                                                                               tgCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAAcc                                  <  1:765446/150‑1 (MQ=255)
                                                                                                                gcgcACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCg                                 <  2:1421954/150‑1 (MQ=255)
                                                                                                                 cgcACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGc                                <  1:1035768/150‑1 (MQ=255)
                                                                                                                 cgcACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGc                                <  2:3572455/150‑1 (MQ=255)
                                                                                                                  gcACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCt                               >  1:4307951/1‑150 (MQ=255)
                                                                                                                     ccGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGc                            <  2:1804987/150‑1 (MQ=255)
                                                                                                                       gTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCa                          >  1:4387757/1‑150 (MQ=255)
                                                                                                                       gTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCa                          <  1:1332959/150‑1 (MQ=255)
                                                                                                                         gCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAg                        <  2:4297266/150‑1 (MQ=255)
                                                                                                                           tttCATCTATCGAACGCAAAAATAATTCTCTCAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTCTAAAAAATCATGTAAAACCGCTCGCCAAGAc                      <  1:423153/150‑1 (MQ=255)
                                                                                                                            ttCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGAcc                     >  1:2410706/1‑150 (MQ=255)
                                                                                                                              cATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGc                   >  1:3907825/1‑150 (MQ=255)
                                                                                                                                      gAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCg           <  2:4420050/150‑1 (MQ=255)
                                                                                                                                         cGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGt        >  2:257431/1‑150 (MQ=255)
                                                                                                                                           cAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTaa      >  1:4996145/1‑150 (MQ=255)
                                                                                                                                           cAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTaa      >  1:1865337/1‑150 (MQ=255)
                                                                                                                                           cAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTaa      <  2:849170/150‑1 (MQ=255)
                                                                                                                                            aaaaaTAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAt     >  1:833622/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTC  >  CP009273/2262991‑2263283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGA  >  CP009273/2262989‑2263285
                                                                                                                                                    |                                                                                                                                                    
AATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAA                                                                                                                                                     >  SRR19973063.1477015/1‑150 (MQ=60)
  TCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATT                                                                                                                                                   >  SRR19973063.1812814/1‑150 (MQ=60)
   CACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTC                                                                                                                                                  >  SRR19973063.855832/1‑150 (MQ=60)
   CACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTC                                                                                                                                                  >  SRR19973063.855728/1‑150 (MQ=60)
         TTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAG                                                                                                                                            <  SRR19973063.2003332/150‑1 (MQ=60)
         TTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAG                                                                                                                                            <  SRR19973063.1238380/150‑1 (MQ=60)
           TTGACAAATCACTACCAGGAAAAAAAATACATTTCAAAATCAAATATCTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTA                                                                                                                                          <  SRR19973063.313438/150‑1 (MQ=60)
               CAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATG                                                                                                                                      <  SRR19973063.5045256/150‑1 (MQ=60)
               CAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATG                                                                                                                                      <  SRR19973063.5045251/150‑1 (MQ=60)
                 AATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAA                                                                                                                                    <  SRR19973063.2373229/150‑1 (MQ=60)
                  ATCACTACCAGGAAAAAACAAACAATTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCTAACGCAAAAATAATTCTCTAAGTAAATGAAT                                                                                                                                   >  SRR19973063.4274138/1‑150 (MQ=60)
                   TCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATG                                                                                                                                  <  SRR19973063.2591078/150‑1 (MQ=60)
                   TCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATG                                                                                                                                  <  SRR19973063.2590996/150‑1 (MQ=60)
                    CTCTACCAGGAAAAAACAAAAATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGG                                                                                                                                 <  SRR19973063.4064457/150‑1 (MQ=60)
                     ACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGA                                                                                                                                >  SRR19973063.2261375/1‑150 (MQ=60)
                     ACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGA                                                                                                                                >  SRR19973063.3726101/1‑150 (MQ=60)
                      CTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGAT                                                                                                                               >  SRR19973063.1272539/1‑150 (MQ=60)
                       TACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATT                                                                                                                              <  SRR19973063.4416444/150‑1 (MQ=60)
                        ACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTG                                                                                                                             >  SRR19973063.3706555/1‑150 (MQ=60)
                        ACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTG                                                                                                                             >  SRR19973063.3706593/1‑150 (MQ=60)
                             GAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGC                                                                                                                        >  SRR19973063.4875572/1‑150 (MQ=60)
                                AAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTT                                                                                                                     >  SRR19973063.2784998/1‑150 (MQ=60)
                                AAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTT                                                                                                                     >  SRR19973063.2247906/1‑150 (MQ=60)
                                AAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTT                                                                                                                     <  SRR19973063.4168608/150‑1 (MQ=60)
                                AAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTT                                                                                                                     <  SRR19973063.4686969/150‑1 (MQ=60)
                                 AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                    <  SRR19973063.1163344/150‑1 (MQ=60)
                                 AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                    <  SRR19973063.1163290/150‑1 (MQ=60)
                                 AAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTT                                                                                                                    <  SRR19973063.532844/150‑1 (MQ=60)
                                  AACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTC                                                                                                                   <  SRR19973063.1304252/150‑1 (MQ=60)
                                     AAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACT                                                                                                                <  SRR19973063.147704/150‑1 (MQ=60)
                                       ACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCA                                                                                                              <  SRR19973063.1418813/150‑1 (MQ=60)
                                         ATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAAT                                                                                                            <  SRR19973063.5022103/150‑1 (MQ=60)
                                           TTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTG                                                                                                          <  SRR19973063.4636649/150‑1 (MQ=60)
                                           TTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTG                                                                                                          <  SRR19973063.3705494/150‑1 (MQ=60)
                                           TTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTG                                                                                                          <  SRR19973063.3222552/150‑1 (MQ=60)
                                           TTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTG                                                                                                          <  SRR19973063.3222745/150‑1 (MQ=60)
                                            TCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGT                                                                                                         >  SRR19973063.2051360/1‑150 (MQ=60)
                                            TCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGT                                                                                                         >  SRR19973063.4857404/1‑150 (MQ=60)
                                             CCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTA                                                                                                        >  SRR19973063.4087921/1‑150 (MQ=60)
                                             CCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTA                                                                                                        <  SRR19973063.3026636/150‑1 (MQ=60)
                                             CCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTA                                                                                                        <  SRR19973063.3026700/150‑1 (MQ=60)
                                             CCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTA                                                                                                        <  SRR19973063.3026737/150‑1 (MQ=60)
                                              CAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGGGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAC                                                                                                       >  SRR19973063.657825/1‑150 (MQ=60)
                                              CAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAC                                                                                                       >  SRR19973063.3578325/1‑150 (MQ=60)
                                              CAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTAC                                                                                                       >  SRR19973063.3578353/1‑150 (MQ=60)
                                                   CAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAA                                                                                                  >  SRR19973063.1810588/1‑150 (MQ=60)
                                                      ATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTG                                                                                               <  SRR19973063.770198/150‑1 (MQ=60)
                                                       TAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGA                                                                                              <  SRR19973063.1126503/150‑1 (MQ=60)
                                                        AACTCACAGTAATTAACATCATCAGTGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGAC                                                                                             <  SRR19973063.2854078/150‑1 (MQ=60)
                                                            CACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAAC                                                                                         >  SRR19973063.393481/1‑150 (MQ=60)
                                                              CAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCC                                                                                       <  SRR19973063.194685/150‑1 (MQ=60)
                                                                 TAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGC                                                                                    >  SRR19973063.4552845/1‑150 (MQ=60)
                                                                     TAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTAT                                                                                <  SRR19973063.1957993/150‑1 (MQ=60)
                                                                      AACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATT                                                                               <  SRR19973063.565103/150‑1 (MQ=60)
                                                                      AACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATT                                                                               >  SRR19973063.3100179/1‑150 (MQ=60)
                                                                           CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                          <  SRR19973063.1992477/150‑1 (MQ=60)
                                                                           CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                          <  SRR19973063.1992426/150‑1 (MQ=60)
                                                                           CATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTT                                                                          <  SRR19973063.3096724/150‑1 (MQ=60)
                                                                            ATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTT                                                                         >  SRR19973063.432940/1‑150 (MQ=60)
                                                                            ATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTT                                                                         >  SRR19973063.3375387/1‑150 (MQ=60)
                                                                               AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCAGTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                      <  SRR19973063.3032673/150‑1 (MQ=60)
                                                                               AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                      >  SRR19973063.5145136/1‑150 (MQ=60)
                                                                               AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                      >  SRR19973063.2007029/1‑150 (MQ=60)
                                                                               AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                      <  SRR19973063.1220844/150‑1 (MQ=60)
                                                                               AGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTG                                                                      <  SRR19973063.2982029/150‑1 (MQ=60)
                                                                                  GTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTAT                                                                   <  SRR19973063.1710223/150‑1 (MQ=60)
                                                                                   TTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATC                                                                  <  SRR19973063.4299900/150‑1 (MQ=60)
                                                                                   TTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATC                                                                  <  SRR19973063.3296103/150‑1 (MQ=60)
                                                                                    TATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCA                                                                 >  SRR19973063.2044977/1‑150 (MQ=60)
                                                                                    TATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTATGTATCA                                                                 >  SRR19973063.2045121/1‑150 (MQ=60)
                                                                                     ATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCAC                                                                >  SRR19973063.4279595/1‑150 (MQ=60)
                                                                                     ATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACAAACCCCGCTTATTAACTTTCTGTATAAC                                                                >  SRR19973063.5098413/1‑150 (MQ=60)
                                                                                       TTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTT                                                              <  SRR19973063.1022877/150‑1 (MQ=60)
                                                                                       TTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTT                                                              <  SRR19973063.1022818/150‑1 (MQ=60)
                                                                                        TTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTT                                                             <  SRR19973063.1223900/150‑1 (MQ=60)
                                                                                        TTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTT                                                             >  SRR19973063.5015433/1‑150 (MQ=60)
                                                                                          TATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTT                                                           >  SRR19973063.2835457/1‑150 (MQ=60)
                                                                                          TATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTT                                                           >  SRR19973063.2835556/1‑150 (MQ=60)
                                                                                          TATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTT                                                           >  SRR19973063.4991470/1‑150 (MQ=60)
                                                                                            TAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCT                                                         >  SRR19973063.851621/1‑150 (MQ=60)
                                                                                            TAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCT                                                         >  SRR19973063.851618/1‑150 (MQ=60)
                                                                                             AGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTT                                                        >  SRR19973063.2966666/1‑150 (MQ=60)
                                                                                             AGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTT                                                        >  SRR19973063.2966775/1‑150 (MQ=60)
                                                                                              GTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTA                                                       <  SRR19973063.3016854/150‑1 (MQ=60)
                                                                                               TGAGTATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGTATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTAT                                                      <  SRR19973063.4409272/150‑1 (MQ=60)
                                                                                               TGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTAT                                                      <  SRR19973063.2556350/150‑1 (MQ=60)
                                                                                                GAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATA                                                     <  SRR19973063.684362/150‑1 (MQ=60)
                                                                                                GAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATA                                                     <  SRR19973063.2356084/150‑1 (MQ=60)
                                                                                                 AGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAA                                                    >  SRR19973063.3900808/1‑150 (MQ=60)
                                                                                                  GGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAA                                                   >  SRR19973063.3583887/1‑150 (MQ=60)
                                                                                                  GGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAA                                                   >  SRR19973063.3583911/1‑150 (MQ=60)
                                                                                                   GATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAA                                                  >  SRR19973063.1137208/1‑150 (MQ=60)
                                                                                                   GATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAA                                                  >  SRR19973063.4597210/1‑150 (MQ=60)
                                                                                                   GATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAA                                                  >  SRR19973063.4597234/1‑150 (MQ=60)
                                                                                                      AATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAAT                                               >  SRR19973063.2315992/1‑150 (MQ=60)
                                                                                                      AATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAAT                                               <  SRR19973063.675646/150‑1 (MQ=60)
                                                                                                         CCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCAT                                            >  SRR19973063.3846793/1‑150 (MQ=60)
                                                                                                         CCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCAT                                            <  SRR19973063.4931235/150‑1 (MQ=60)
                                                                                                          CTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATG                                           >  SRR19973063.4016404/1‑150 (MQ=60)
                                                                                                           TGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGT                                          >  SRR19973063.1771446/1‑150 (MQ=60)
                                                                                                             ATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAA                                        <  SRR19973063.4427502/150‑1 (MQ=60)
                                                                                                             ATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAA                                        >  SRR19973063.1696460/1‑150 (MQ=60)
                                                                                                             ATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAA                                        >  SRR19973063.4924614/1‑150 (MQ=60)
                                                                                                             ATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTCCTTATAAAAAATCATGTAA                                        >  SRR19973063.3917009/1‑150 (MQ=60)
                                                                                                                 TGCGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACC                                    <  SRR19973063.767681/150‑1 (MQ=60)
                                                                                                                   CGCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGC                                  <  SRR19973063.1038905/150‑1 (MQ=60)
                                                                                                                   CGCACCGTGCTTTCATCTATCGAACGAAATAATAATTCTCTAAGTAATTGAATCCATTGCATGAGTTTCACTAAATTTAAATTTAAATGACCAACCCCGCTTATTAACTATCTGTATCACTTTATCTTATAAAAAATCATTTAAAACCta                                  >  SRR19973063.5052255/1‑148 (MQ=60)
                                                                                                                    GCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCT                                 >  SRR19973063.3603912/1‑150 (MQ=60)
                                                                                                                    GCACCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCT                                 >  SRR19973063.4321639/1‑150 (MQ=60)
                                                                                                                       CCGTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGC                              >  SRR19973063.4510500/1‑150 (MQ=60)
                                                                                                                         GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCA                            >  SRR19973063.4401810/1‑150 (MQ=60)
                                                                                                                         GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCA                            <  SRR19973063.389429/150‑1 (MQ=60)
                                                                                                                         GTGCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCA                            <  SRR19973063.1336955/150‑1 (MQ=60)
                                                                                                                           GCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAG                          <  SRR19973063.3935258/150‑1 (MQ=60)
                                                                                                                           GCTTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAG                          <  SRR19973063.2530668/150‑1 (MQ=60)
                                                                                                                             TTTCATCTATCGAACGCAAAAATAATTCTCTCAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTCTAAAAAATCATGTAAAACCGCTCGCCAAGAC                        <  SRR19973063.424337/150‑1 (MQ=60)
                                                                                                                             TTTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGAC                        >  SRR19973063.1284337/1‑150 (MQ=60)
                                                                                                                              TTCATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACC                       >  SRR19973063.2418419/1‑150 (MQ=60)
                                                                                                                                CATCTATCGAACGCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGC                     >  SRR19973063.3920284/1‑150 (MQ=60)
                                                                                                                                            GCAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTA         >  SRR19973063.577088/1‑150 (MQ=60)
                                                                                                                                             CAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAA        >  SRR19973063.1871165/1‑150 (MQ=60)
                                                                                                                                             CAAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAA        >  SRR19973063.5012281/1‑150 (MQ=60)
                                                                                                                                              AAAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAAT       >  SRR19973063.836033/1‑150 (MQ=60)
                                                                                                                                               AAAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATC      <  SRR19973063.4825252/150‑1 (MQ=60)
                                                                                                                                                AAATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCT     <  SRR19973063.2576394/150‑1 (MQ=60)
                                                                                                                                                 AATAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCCAGACCGCACCAATCGGGTAATCTC    >  SRR19973063.2471006/1‑150 (MQ=60)
                                                                                                                                                   TAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGA  >  SRR19973063.5039521/1‑150 (MQ=60)
                                                                                                                                                   TAATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGA  >  SRR19973063.4372458/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
AATCACCTTTTTTGACAAATCACTACCAGGAAAAAACAAACATTTCCAAAACAAATAACTCACAGTAATTAACATCATCAGGGTTATTTTTATAGTGAGGATAATCCTGATGATGCGCACCGTGCTTTCATCTATCGAACGCAAAAATCATTCTCTAAGTAAATGAATGGATTGCATGCGTTTCACTCAATTGTACTTTAATTGACCAACCCCGCTTATTAACTTTCTGTATCACTTTTTCTTATAAAAAATCATGTAAAACCGCTCGCCAAGACCGCACCAATCGGGTAATCTCGA  >  CP009273/2262989‑2263285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑