Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,083,443 G→T 100% S624* (TCG→TAG)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,083,4430GT98.2% 715.2 / 3.2 225S624* (TCG→TAG) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (116/105);  total (117/108)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

ATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT  >  CP009273/1083298‑1083587
                                                                                                                                                 |                                                                                                                                                
aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaa                                                                                                                                                >  1:730489/1‑148 (MQ=255)
 tCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaag                                                                                                                                               <  2:360160/148‑1 (MQ=255)
   aTTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaagaa                                                                                                                                             <  1:1855607/148‑1 (MQ=255)
    tttttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTAAGTTGTAATAGGATCATCCAGTGAGCGAGTTGTTGCTAAGaaa                                                                                                                                            >  2:458219/1‑148 (MQ=255)
       ttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAg                                                                                                                                         <  2:1624220/148‑1 (MQ=255)
       ttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAg                                                                                                                                         <  1:2041985/148‑1 (MQ=255)
            aTACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGa                                                                                                                                    <  1:555034/148‑1 (MQ=255)
             tACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGAt                                                                                                                                   >  2:170829/1‑148 (MQ=255)
             tACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGAt                                                                                                                                   >  2:173261/1‑148 (MQ=255)
                cAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCt                                                                                                                                <  1:3469135/148‑1 (MQ=255)
                      gTTGAAAACTCAGGACGAATAAGGTCTATTTCATGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAt                                                                                                                          <  2:1311550/148‑1 (MQ=255)
                       ttGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAtt                                                                                                                         >  1:2155470/1‑148 (MQ=255)
                         gAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAtttt                                                                                                                       <  1:2380098/148‑1 (MQ=255)
                              atCAGGACGAATATGTTCTATTTCATGTTTGTTATTCAGAAAATTGTAGGGATAATAACCATAGTTTTTAACTCCATTCAGTTGTAATAGTCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGc                                                                                                                  <  2:2033974/147‑1 (MQ=255)
                                 aGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAg                                                                                                               <  2:2451046/148‑1 (MQ=255)
                                 aGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAg                                                                                                               >  1:1216449/1‑148 (MQ=255)
                                  ggACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGt                                                                                                              >  2:3237319/1‑148 (MQ=255)
                                  ggACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGt                                                                                                              <  2:1191556/148‑1 (MQ=255)
                                   gACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGtt                                                                                                             >  2:336098/1‑148 (MQ=255)
                                   gACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGtt                                                                                                             <  1:3539302/148‑1 (MQ=255)
                                        aTAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTtgtg                                                                                                        <  2:3456415/148‑1 (MQ=255)
                                          aaGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGcc                                                                                                      <  2:315136/148‑1 (MQ=255)
                                           aGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCt                                                                                                     >  2:792109/1‑148 (MQ=255)
                                             gTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGt                                                                                                   >  1:1078547/1‑148 (MQ=255)
                                             gTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGt                                                                                                   <  1:1612349/148‑1 (MQ=255)
                                              tCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTa                                                                                                  >  1:1925975/1‑148 (MQ=255)
                                               cTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTaa                                                                                                 >  1:2309673/1‑148 (MQ=255)
                                               cTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTaa                                                                                                 >  1:448200/1‑148 (MQ=255)
                                               cTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTaa                                                                                                 <  1:170829/148‑1 (MQ=255)
                                               cTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTaa                                                                                                 <  1:173261/148‑1 (MQ=255)
                                                 aTTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAAtt                                                                                               >  2:418052/1‑148 (MQ=255)
                                                   ttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCt                                                                                             >  2:934054/1‑148 (MQ=255)
                                                   ttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCt                                                                                             >  1:1442474/1‑148 (MQ=255)
                                                     cAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTaa                                                                                           >  2:2827841/1‑148 (MQ=255)
                                                      aGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTaaa                                                                                          <  2:469875/148‑1 (MQ=255)
                                                       ggttggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTaaaa                                                                                         <  2:232774/148‑1 (MQ=255)
                                                         ttggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATa                                                                                       >  2:1567141/1‑148 (MQ=255)
                                                          tggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAg                                                                                      >  1:49525/1‑148 (MQ=255)
                                                            gttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAt                                                                                    <  2:231277/148‑1 (MQ=255)
                                                            gttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAt                                                                                    <  2:256766/148‑1 (MQ=255)
                                                            gttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAt                                                                                    <  2:2103410/148‑1 (MQ=255)
                                                            gttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAt                                                                                    <  2:1864751/148‑1 (MQ=255)
                                                               aTGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTACATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTg                                                                                 <  2:39168/148‑1 (MQ=255)
                                                                 gCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTc                                                                               >  2:632708/1‑148 (MQ=255)
                                                                  cAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCt                                                                              <  1:2876564/148‑1 (MQ=255)
                                                                        aTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGcc                                                                        >  2:2398582/1‑148 (MQ=255)
                                                                         ttGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCt                                                                       >  1:627999/1‑148 (MQ=255)
                                                                         ttGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCt                                                                       >  1:2418098/1‑148 (MQ=255)
                                                                            tCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAg                                                                    >  2:2194600/1‑148 (MQ=255)
                                                                            tCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAg                                                                    >  1:2679851/1‑148 (MQ=255)
                                                                            tCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAg                                                                    >  1:827622/1‑148 (MQ=255)
                                                                             cGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAgg                                                                   <  1:6834/148‑1 (MQ=255)
                                                                             cGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAgg                                                                   <  2:2496641/148‑1 (MQ=255)
                                                                              gggATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAggg                                                                  <  1:304155/148‑1 (MQ=255)
                                                                               ggATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGa                                                                 <  2:308610/148‑1 (MQ=255)
                                                                               ggATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGa                                                                 >  2:795397/1‑148 (MQ=255)
                                                                                 atactaACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATg                                                               <  2:3138273/148‑1 (MQ=255)
                                                                                   aataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtt                                                             <  1:485286/148‑1 (MQ=255)
                                                                                      aaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttttt                                                          >  1:3266255/1‑148 (MQ=255)
                                                                                        ccATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTaa                                                        <  2:2679851/148‑1 (MQ=255)
                                                                                        ccATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTaa                                                        >  1:3138009/1‑148 (MQ=255)
                                                                                         cATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAt                                                       <  1:1003622/148‑1 (MQ=255)
                                                                                         cATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAt                                                       <  1:2476823/148‑1 (MQ=255)
                                                                                         cATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAt                                                       >  1:3293654/1‑148 (MQ=255)
                                                                                          aTAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAtt                                                      <  1:974095/148‑1 (MQ=255)
                                                                                           tAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAttt                                                     >  1:1937492/1‑148 (MQ=255)
                                                                                             gTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGa                                                   <  2:3285331/148‑1 (MQ=255)
                                                                                               ttttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAtt                                                 <  2:3516657/148‑1 (MQ=255)
                                                                                                tttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGACAGAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTg                                                <  2:1844068/148‑1 (MQ=255)
                                                                                                tttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTg                                                >  1:761293/1‑148 (MQ=255)
                                                                                                tttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTg                                                >  1:906520/1‑148 (MQ=255)
                                                                                                  tCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGt                                              <  2:3423184/148‑1 (MQ=255)
                                                                                                      tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAt                                          >  1:3345719/1‑148 (MQ=255)
                                                                                                        cATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGACTGAGGGATGTTTTTAATTTGATTGGTCAATTg                                        <  1:2027401/148‑1 (MQ=255)
                                                                                                         aTTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGt                                       >  2:3278345/1‑148 (MQ=255)
                                                                                                                tGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAAcc                                <  1:2232151/148‑1 (MQ=255)
                                                                                                                tGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAAcc                                <  2:2541805/148‑1 (MQ=255)
                                                                                                                 gTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCa                               <  1:2273738/148‑1 (MQ=255)
                                                                                                                  tAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                              >  1:1641176/1‑148 (MQ=255)
                                                                                                                  tAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                              >  1:1311825/1‑148 (MQ=255)
                                                                                                                  tAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                              <  2:216260/148‑1 (MQ=255)
                                                                                                                   aaTAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAtt                             >  2:1490375/1‑148 (MQ=255)
                                                                                                                   aaTAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAtt                             >  1:1923/1‑148 (MQ=255)
                                                                                                                    aTAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTg                            >  2:1327577/1‑148 (MQ=255)
                                                                                                                      aGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGt                          >  1:516614/1‑148 (MQ=255)
                                                                                                                       ggCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTc                         >  1:938591/1‑148 (MQ=255)
                                                                                                                        gCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCa                        >  2:273246/1‑148 (MQ=255)
                                                                                                                        gCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCa                        >  2:2074135/1‑148 (MQ=255)
                                                                                                                             tCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGa                   <  2:2248469/148‑1 (MQ=255)
                                                                                                                              ccAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAt                  <  1:2640295/148‑1 (MQ=255)
                                                                                                                              ccAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAt                  <  1:3236833/148‑1 (MQ=255)
                                                                                                                              ccAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATAGGTCAGCCGAt                  <  1:1891502/148‑1 (MQ=255)
                                                                                                                                   gAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTc             <  1:3324617/148‑1 (MQ=255)
                                                                                                                                      cGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGc          >  1:1827667/1‑148 (MQ=255)
                                                                                                                                      cGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGc          >  1:1386072/1‑148 (MQ=255)
                                                                                                                                        aGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGa        <  1:2948698/148‑1 (MQ=255)
                                                                                                                                        aGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGa        >  1:2051808/1‑148 (MQ=255)
                                                                                                                                         gttgttGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGac       >  1:3312415/1‑148 (MQ=255)
                                                                                                                                          ttgttgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGaca      >  1:1118687/1‑148 (MQ=255)
                                                                                                                                           tgttgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGacac     <  2:2928242/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT  >  CP009273/1083298‑1083587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAG  >  CP009273/1083294‑1083591
                                                                                                                                                     |                                                                                                                                                    
ATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCT                                                                                                                                                      <  SRR19973066.101571/150‑1 (MQ=60)
ATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCT                                                                                                                                                      >  SRR19973066.1627382/1‑150 (MQ=60)
 TTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTA                                                                                                                                                     <  SRR19973066.1880647/150‑1 (MQ=60)
 TTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTA                                                                                                                                                     <  SRR19973066.64105/150‑1 (MQ=60)
  TAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAA                                                                                                                                                    >  SRR19973066.735906/1‑150 (MQ=60)
      CATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAA                                                                                                                                                >  SRR19973066.2089059/1‑150 (MQ=60)
      CATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAA                                                                                                                                                >  SRR19973066.3540081/1‑150 (MQ=60)
       ATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAAT                                                                                                                                               <  SRR19973066.1868890/150‑1 (MQ=60)
       ATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAAT                                                                                                                                               <  SRR19973066.1868883/150‑1 (MQ=60)
           TTCGGATACCAGTCTGTTGATAACTCAGGACGAATATGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTAGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCATTGAGCGAGTTGTTGCTAAGAAATAGCC                                                                                                                                           <  SRR19973066.2356177/150‑1 (MQ=60)
           TTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCC                                                                                                                                           <  SRR19973066.2056162/150‑1 (MQ=60)
           TTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCC                                                                                                                                           <  SRR19973066.2056202/150‑1 (MQ=60)
            TCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCT                                                                                                                                          <  SRR19973066.3319896/150‑1 (MQ=60)
              GGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGA                                                                                                                                        >  SRR19973066.1234848/1‑150 (MQ=60)
                ATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATG                                                                                                                                      <  SRR19973066.559013/150‑1 (MQ=60)
                 TACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAACTAGCCTGATGC                                                                                                                                     >  SRR19973066.2018440/1‑150 (MQ=60)
                    CAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGA                                                                                                                                  <  SRR19973066.3492315/150‑1 (MQ=60)
                      GGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACC                                                                                                                                >  SRR19973066.627661/1‑150 (MQ=60)
                        CTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTCGTTATGCAGAAAAGTGTCGGGATAATAACCATAGATTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGAGAGTTGTTGCTAAGAAATATCCTGAcatcgcaaga                                                                                                                              >  SRR19973066.706096/1‑140 (MQ=60)
                         TGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTTCTAAGAAATAGCCTGATGCTGACCATT                                                                                                                             >  SRR19973066.1940850/1‑150 (MQ=60)
                         TGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATT                                                                                                                             >  SRR19973066.2170382/1‑150 (MQ=60)
                         TGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATT                                                                                                                             >  SRR19973066.2170437/1‑150 (MQ=60)
                             GAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTC                                                                                                                         <  SRR19973066.2396154/150‑1 (MQ=60)
                              AAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCT                                                                                                                        <  SRR19973066.1729969/150‑1 (MQ=60)
                               AAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTG                                                                                                                       <  SRR19973066.2606505/150‑1 (MQ=60)
                                AACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGC                                                                                                                      >  SRR19973066.3304477/1‑150 (MQ=60)
                                   TCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAG                                                                                                                   <  SRR19973066.723988/150‑1 (MQ=60)
                                   TCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAG                                                                                                                   <  SRR19973066.3357939/150‑1 (MQ=60)
                                   TCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAG                                                                                                                   >  SRR19973066.1225013/1‑150 (MQ=60)
                                       GACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTT                                                                                                               <  SRR19973066.3562822/150‑1 (MQ=60)
                                            ATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCC                                                                                                          >  SRR19973066.1230883/1‑150 (MQ=60)
                                               AGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGT                                                                                                       >  SRR19973066.1086432/1‑150 (MQ=60)
                                                GGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTA                                                                                                      >  SRR19973066.1939671/1‑150 (MQ=60)
                                                 GTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAA                                                                                                     >  SRR19973066.2325483/1‑150 (MQ=60)
                                                 GTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAA                                                                                                     >  SRR19973066.451656/1‑150 (MQ=60)
                                                 GTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAA                                                                                                     <  SRR19973066.1624044/150‑1 (MQ=60)
                                                   CTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATT                                                                                                   <  SRR19973066.172250/150‑1 (MQ=60)
                                                   CTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATT                                                                                                   <  SRR19973066.172356/150‑1 (MQ=60)
                                                   CTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATT                                                                                                   <  SRR19973066.174802/150‑1 (MQ=60)
                                                    TATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTC                                                                                                  >  SRR19973066.2543628/1‑150 (MQ=60)
                                                    TATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTC                                                                                                  >  SRR19973066.744464/1‑150 (MQ=60)
                                                     ATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCT                                                                                                 >  SRR19973066.1452739/1‑150 (MQ=60)
                                                     ATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCT                                                                                                 >  SRR19973066.1937689/1‑150 (MQ=60)
                                                           GGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATA                                                                                           <  SRR19973066.97691/150‑1 (MQ=60)
                                                           GGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATA                                                                                           <  SRR19973066.1051720/150‑1 (MQ=60)
                                                            GTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAG                                                                                          >  SRR19973066.50161/1‑150 (MQ=60)
                                                              TGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAT                                                                                        >  SRR19973066.2948763/1‑150 (MQ=60)
                                                              TGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAT                                                                                        <  SRR19973066.2454145/150‑1 (MQ=60)
                                                              TGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAT                                                                                        <  SRR19973066.1918006/150‑1 (MQ=60)
                                                               GGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATCGATT                                                                                       >  SRR19973066.797569/1‑150 (MQ=60)
                                                               GGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATT                                                                                       >  SRR19973066.3205418/1‑150 (MQ=60)
                                                               GGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTATTTGCTAAGAAATAGCCTGATGCTGAACATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATA                                                                                       >  SRR19973066.299135/1‑150 (MQ=60)
                                                                GTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTT                                                                                      <  SRR19973066.591844/150‑1 (MQ=60)
                                                                 TTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTG                                                                                     <  SRR19973066.638046/150‑1 (MQ=60)
                                                                      CAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTT                                                                                <  SRR19973066.2895836/150‑1 (MQ=60)
                                                                       AGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTA                                                                               >  SRR19973066.463551/1‑150 (MQ=60)
                                                                           AATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCT                                                                           >  SRR19973066.632497/1‑150 (MQ=60)
                                                                           AATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCT                                                                           >  SRR19973066.2434497/1‑150 (MQ=60)
                                                                              TGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAG                                                                        >  SRR19973066.833522/1‑150 (MQ=60)
                                                                              TGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAG                                                                        >  SRR19973066.2697739/1‑150 (MQ=60)
                                                                              TGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAG                                                                        >  SRR19973066.20438/1‑150 (MQ=60)
                                                                              TGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAG                                                                        >  SRR19973066.833658/1‑150 (MQ=60)
                                                                               GTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGG                                                                       >  SRR19973066.3547534/1‑150 (MQ=60)
                                                                                TCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGG                                                                      <  SRR19973066.1493957/150‑1 (MQ=60)
                                                                                 CGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGA                                                                     <  SRR19973066.6945/150‑1 (MQ=60)
                                                                                 CGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGA                                                                     <  SRR19973066.2766976/150‑1 (MQ=60)
                                                                                 CGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGA                                                                     <  SRR19973066.6950/150‑1 (MQ=60)
                                                                                  GGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGAT                                                                    <  SRR19973066.306325/150‑1 (MQ=60)
                                                                                     ATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTT                                                                 >  SRR19973066.2372962/1‑150 (MQ=60)
                                                                                       AATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTT                                                               <  SRR19973066.488931/150‑1 (MQ=60)
                                                                                       AATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTT                                                               <  SRR19973066.490078/150‑1 (MQ=60)
                                                                                        ATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTT                                                              >  SRR19973066.3287717/1‑150 (MQ=60)
                                                                                        ATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTT                                                              >  SRR19973066.3287555/1‑150 (MQ=60)
                                                                                          AACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAA                                                            >  SRR19973066.3158949/1‑150 (MQ=60)
                                                                                           ACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAT                                                           >  SRR19973066.2312365/1‑150 (MQ=60)
                                                                                           ACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAT                                                           >  SRR19973066.3315395/1‑150 (MQ=60)
                                                                                             CATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTT                                                         <  SRR19973066.369770/150‑1 (MQ=60)
                                                                                             CATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTT                                                         <  SRR19973066.1011013/150‑1 (MQ=60)
                                                                                             CATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTT                                                         <  SRR19973066.2493721/150‑1 (MQ=60)
                                                                                             CATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTT                                                         >  SRR19973066.1951238/1‑150 (MQ=60)
                                                                                              ATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTG                                                        <  SRR19973066.981262/150‑1 (MQ=60)
                                                                                                 GTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATT                                                     >  SRR19973066.503698/1‑150 (MQ=60)
                                                                                                  TTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTG                                                    >  SRR19973066.766938/1‑150 (MQ=60)
                                                                                                  TTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTG                                                    >  SRR19973066.912922/1‑150 (MQ=60)
                                                                                                      TCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCA                                                >  SRR19973066.2213531/1‑150 (MQ=60)
                                                                                                        ACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTTATTTGATTGGTCAAT                                              <  SRR19973066.334585/150‑1 (MQ=60)
                                                                                                        ACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTTATTTGATTGGTCAAT                                              <  SRR19973066.334588/150‑1 (MQ=60)
                                                                                                        ACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAT                                              >  SRR19973066.1587526/1‑150 (MQ=60)
                                                                                                        ACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAT                                              >  SRR19973066.3367992/1‑150 (MQ=60)
                                                                                                            CATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTA                                          <  SRR19973066.1406605/150‑1 (MQ=60)
                                                                                                            CATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTA                                          >  SRR19973066.2058872/1‑150 (MQ=60)
                                                                                                            CATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTA                                          <  SRR19973066.1216767/150‑1 (MQ=60)
                                                                                                            CATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGACTGAGGGATGTTTTTAATTTGATTGGTCAATTGTA                                          <  SRR19973066.2041569/150‑1 (MQ=60)
                                                                                                               TCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTA                                       <  SRR19973066.839656/150‑1 (MQ=60)
                                                                                                                 AGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAAC                                     >  SRR19973066.1113050/1‑150 (MQ=60)
                                                                                                                    TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                                  >  SRR19973066.1321346/1‑150 (MQ=60)
                                                                                                                    TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                                  <  SRR19973066.2247561/150‑1 (MQ=60)
                                                                                                                    TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                                  >  SRR19973066.1321725/1‑150 (MQ=60)
                                                                                                                    TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                                  >  SRR19973066.1653112/1‑150 (MQ=60)
                                                                                                                    TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                                  >  SRR19973066.943712/1‑150 (MQ=60)
                                                                                                                     GTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATT                                 >  SRR19973066.1935/1‑150 (MQ=60)
                                                                                                                     GTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATT                                 >  SRR19973066.1951/1‑150 (MQ=60)
                                                                                                                     GTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATT                                 >  SRR19973066.2287308/1‑150 (MQ=60)
                                                                                                                     GTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATT                                 <  SRR19973066.2289364/150‑1 (MQ=60)
                                                                                                                        ATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGT                              >  SRR19973066.520193/1‑150 (MQ=60)
                                                                                                                        ATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGT                              >  SRR19973066.520421/1‑150 (MQ=60)
                                                                                                                         TAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTC                             >  SRR19973066.945421/1‑150 (MQ=60)
                                                                                                                              TCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCG                        <  SRR19973066.3096229/150‑1 (MQ=60)
                                                                                                                                ATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAT                      >  SRR19973066.1815928/1‑150 (MQ=60)
                                                                                                                                 TCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATT                     >  SRR19973066.2660766/1‑150 (MQ=60)
                                                                                                                                  CCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTT                    <  SRR19973066.3258201/150‑1 (MQ=60)
                                                                                                                                  CCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTT                    <  SRR19973066.3258691/150‑1 (MQ=60)
                                                                                                                                  CCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTT                    <  SRR19973066.2658059/150‑1 (MQ=60)
                                                                                                                                  CCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATAGGTCAGCCGATTT                    <  SRR19973066.1904986/150‑1 (MQ=60)
                                                                                                                                    AGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTT                  >  SRR19973066.3424976/1‑150 (MQ=60)
                                                                                                                                      TGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCT                <  SRR19973066.2411218/150‑1 (MQ=60)
                                                                                                                                       GAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTG               <  SRR19973066.3346706/150‑1 (MQ=60)
                                                                                                                                        AGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGC              >  SRR19973066.1396084/1‑150 (MQ=60)
                                                                                                                                        AGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGC              >  SRR19973066.1840710/1‑150 (MQ=60)
                                                                                                                                          CGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGA            >  SRR19973066.2066063/1‑150 (MQ=60)
                                                                                                                                           GAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGAC           >  SRR19973066.3334395/1‑150 (MQ=60)
                                                                                                                                            AGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACA          <  SRR19973066.1868575/150‑1 (MQ=60)
                                                                                                                                            AGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACA          >  SRR19973066.1126750/1‑150 (MQ=60)
                                                                                                                                            AGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACA          <  SRR19973066.2967400/150‑1 (MQ=60)
                                                                                                                                            AGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACA          <  SRR19973066.2968264/150‑1 (MQ=60)
                                                                                                                                                GTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT      <  SRR19973066.520949/150‑1 (MQ=60)
                                                                                                                                                GTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT      <  SRR19973066.174341/150‑1 (MQ=60)
                                                                                                                                                GTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT      <  SRR19973066.1628064/150‑1 (MQ=60)
                                                                                                                                                    CTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAG  <  SRR19973066.932945/150‑1 (MQ=60)
                                                                                                                                                    CTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAG  >  SRR19973066.1768561/1‑150 (MQ=60)
                                                                                                                                                    CTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAG  <  SRR19973066.2014595/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
ATTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAG  >  CP009273/1083294‑1083591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑