Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,083,443 G→T 100% S624* (TCG→TAG)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,083,4430GT98.3% 731.1 / 1.9 230S624* (TCG→TAG) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (1/3);  new base T (116/110);  total (117/113)
Fisher's exact test for biased strand distribution p-value = 3.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 239 total aligned reads displayed.

ATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT  >  CP009273/1083298‑1083587
                                                                                                                                                 |                                                                                                                                                
aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaa                                                                                                                                                >  2:1106115/1‑148 (MQ=255)
aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaa                                                                                                                                                >  2:774813/1‑148 (MQ=255)
  cATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaaga                                                                                                                                              >  2:659387/1‑148 (MQ=255)
   aTTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaagaa                                                                                                                                             <  2:2976237/148‑1 (MQ=255)
       ttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAg                                                                                                                                         <  2:198872/148‑1 (MQ=255)
       ttCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAg                                                                                                                                         >  1:209531/1‑148 (MQ=255)
           gATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTg                                                                                                                                     <  1:1549543/148‑1 (MQ=255)
            aTACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGa                                                                                                                                    <  1:947481/148‑1 (MQ=255)
             tACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGAt                                                                                                                                   >  1:3107315/1‑148 (MQ=255)
              aCCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATg                                                                                                                                  <  1:774813/148‑1 (MQ=255)
               ccAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGc                                                                                                                                 <  1:1448534/148‑1 (MQ=255)
               ccAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGc                                                                                                                                 <  1:505733/148‑1 (MQ=255)
                 aGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTg                                                                                                                               <  2:2027550/148‑1 (MQ=255)
                 aGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTg                                                                                                                               <  2:1749850/148‑1 (MQ=255)
                   gCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGAc                                                                                                                             >  1:3507704/1‑148 (MQ=255)
                    cTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGAcc                                                                                                                            >  1:307849/1‑148 (MQ=255)
                     tGTTGAAAACTGAGTTCTAATAAGGTCTATTTAAGGTTGGTTATTCAGAAAATTGTCGGGATAATAACATTATTTTTTCACTACATTAATTTGTAATACGCTCATCAAGTGTTCGAGTTTTTGCTAATAAATAGCCTGATTCTGTCCa                                                                                                                           <  2:1926881/148‑1 (MQ=255)
                      gTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAt                                                                                                                          <  1:2026446/148‑1 (MQ=255)
                          aaaaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAttttt                                                                                                                      <  2:1681833/148‑1 (MQ=255)
                           aaaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTc                                                                                                                     <  2:1916892/148‑1 (MQ=255)
                           aaaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTc                                                                                                                     >  2:2799636/1‑148 (MQ=255)
                               tCATGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGcc                                                                                                                 <  2:2336738/148‑1 (MQ=255)
                                        aTAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTtgtg                                                                                                        <  2:2617128/148‑1 (MQ=255)
                                          aaGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGcc                                                                                                      <  2:636472/148‑1 (MQ=255)
                                           aGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCt                                                                                                     >  2:1392417/1‑148 (MQ=255)
                                           aGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCt                                                                                                     <  2:895269/148‑1 (MQ=255)
                                           aGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCt                                                                                                     >  2:2524273/1‑148 (MQ=255)
                                             gTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGt                                                                                                   >  2:2192118/1‑148 (MQ=255)
                                              tCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTa                                                                                                  <  1:237661/148‑1 (MQ=255)
                                               cTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTaa                                                                                                 <  1:2587458/148‑1 (MQ=255)
                                                tATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAAt                                                                                                >  1:1644702/1‑148 (MQ=255)
                                                  tttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTATGCCAGTTTTGTGCCTGTAATTc                                                                                              >  1:3440377/1‑148 (MQ=255)
                                                   ttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCt                                                                                             >  1:612030/1‑148 (MQ=255)
                                                   ttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCt                                                                                             >  2:2583109/1‑148 (MQ=255)
                                                   ttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCt                                                                                             >  2:807791/1‑148 (MQ=255)
                                                     cAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTaa                                                                                           >  1:3399318/1‑148 (MQ=255)
                                                      aGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTaaa                                                                                          <  1:3186876/148‑1 (MQ=255)
                                                         ttggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATa                                                                                       <  2:3428395/148‑1 (MQ=255)
                                                          tggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAg                                                                                      >  1:1321634/1‑148 (MQ=255)
                                                            gttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAt                                                                                    <  1:1156874/148‑1 (MQ=255)
                                                            gttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAt                                                                                    >  2:2290511/1‑148 (MQ=255)
                                                             ttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAtt                                                                                   <  1:2140380/148‑1 (MQ=255)
                                                              tATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAttt                                                                                  >  1:2208959/1‑148 (MQ=255)
                                                              tATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAttt                                                                                  <  2:2251352/148‑1 (MQ=255)
                                                                 gCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTc                                                                               <  2:1728935/148‑1 (MQ=255)
                                                                  cAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTATGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCt                                                                              <  1:1963450/148‑1 (MQ=255)
                                                                     aaaaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTa                                                                           <  2:2893248/148‑1 (MQ=255)
                                                                        aTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGcc                                                                        >  2:2806799/1‑148 (MQ=255)
                                                                        aTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGcc                                                                        >  1:2938949/1‑148 (MQ=255)
                                                                        aTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGcc                                                                        >  1:2506677/1‑148 (MQ=255)
                                                                        aTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGcc                                                                        <  1:993939/148‑1 (MQ=255)
                                                                          tGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTg                                                                      <  1:1106115/148‑1 (MQ=255)
                                                                          tGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTg                                                                      >  1:2012283/1‑148 (MQ=255)
                                                                               ggATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGa                                                                 <  1:1695633/148‑1 (MQ=255)
                                                                                 ataataACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATg                                                               >  2:1577642/1‑148 (MQ=255)
                                                                                   aataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtt                                                             >  2:449296/1‑148 (MQ=255)
                                                                                    ataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTNATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttt                                                            <  1:1784577/148‑1 (MQ=255)
                                                                                    ataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttt                                                            <  1:878122/148‑1 (MQ=255)
                                                                                    ataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttt                                                            <  1:1082270/148‑1 (MQ=255)
                                                                                    ataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttt                                                            <  2:792516/148‑1 (MQ=255)
                                                                                       aCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTa                                                         >  2:2142364/1‑148 (MQ=255)
                                                                                          aTAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAtt                                                      >  1:1631970/1‑148 (MQ=255)
                                                                                             gTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGa                                                   <  1:3347210/148‑1 (MQ=255)
                                                                                             gTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGa                                                   <  1:1982550/148‑1 (MQ=255)
                                                                                              tttttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAt                                                  >  1:969543/1‑148 (MQ=255)
                                                                                              tttttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAt                                                  >  1:2340931/1‑148 (MQ=255)
                                                                                              tttttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAt                                                  <  1:2446862/148‑1 (MQ=255)
                                                                                                tttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTg                                                <  2:1926390/148‑1 (MQ=255)
                                                                                                      tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAt                                          <  1:1500269/148‑1 (MQ=255)
                                                                                                      tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAt                                          >  2:1240445/1‑148 (MQ=255)
                                                                                                      tCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAt                                          <  1:3239552/148‑1 (MQ=255)
                                                                                                       ccATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAtt                                         >  1:274316/1‑148 (MQ=255)
                                                                                                             aGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTa                                   <  1:1392417/148‑1 (MQ=255)
                                                                                                             aGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTa                                   <  1:2600076/148‑1 (MQ=255)
                                                                                                              gTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTaa                                  <  1:2252114/148‑1 (MQ=255)
                                                                                                               ttGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAAc                                 >  1:3360072/1‑148 (MQ=255)
                                                                                                                  tAAGAGGCTCATCCAGTGAGCGAGTTTGAGCTAAGAAAGAGCCTGATGCTGACCATTTGTCAGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTGAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                              <  2:309354/148‑1 (MQ=255)
                                                                                                                     tAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTgg                           <  2:209838/148‑1 (MQ=255)
                                                                                                                       ggCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTc                         <  2:3152028/148‑1 (MQ=255)
                                                                                                                       ggCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTc                         >  1:478561/1‑148 (MQ=255)
                                                                                                                       ggCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTc                         >  2:2750821/1‑148 (MQ=255)
                                                                                                                         cTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAg                       >  1:2237788/1‑148 (MQ=255)
                                                                                                                          tCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGc                      <  2:2109646/148‑1 (MQ=255)
                                                                                                                              ccAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAt                  >  2:2183701/1‑148 (MQ=255)
                                                                                                                                aGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAttt                <  2:875163/148‑1 (MQ=255)
                                                                                                                                 gTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAtttt               >  1:1970010/1‑148 (MQ=255)
                                                                                                                                 gTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAtttt               >  2:2492296/1‑148 (MQ=255)
                                                                                                                                 gTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAtttt               <  1:826276/148‑1 (MQ=255)
                                                                                                                                    aGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCt            >  2:425747/1‑148 (MQ=255)
                                                                                                                                    aGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCt            >  2:425516/1‑148 (MQ=255)
                                                                                                                                     gCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTg           >  2:1806110/1‑148 (MQ=255)
                                                                                                                                     gCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTg           >  2:382245/1‑148 (MQ=255)
                                                                                                                                     gCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTg           >  2:305298/1‑148 (MQ=255)
                                                                                                                                        aGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGa        <  1:655315/148‑1 (MQ=255)
                                                                                                                                         gttgttGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGac       >  1:2484177/1‑148 (MQ=255)
                                                                                                                                           tgttgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGacac     >  1:1192099/1‑148 (MQ=255)
                                                                                                                                           tgttgCTAAGAAATAGCCTGATGATGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTTTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTAAGCCGATTTTTCTGCGacac     >  1:2273597/1‑148 (MQ=255)
                                                                                                                                              tgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCtt  >  1:2804059/1‑148 (MQ=255)
                                                                                                                                              tgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCtt  >  1:2885420/1‑148 (MQ=255)
                                                                                                                                              tgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCtt  <  2:2434402/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT  >  CP009273/1083298‑1083587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAG  >  CP009273/1083295‑1083591
                                                                                                                                                    |                                                                                                                                                    
TTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTA                                                                                                                                                     <  SRR19973065.3384544/150‑1 (MQ=60)
 TAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAA                                                                                                                                                    >  SRR19973065.755366/1‑150 (MQ=60)
  AATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAG                                                                                                                                                   >  SRR19973065.1649529/1‑150 (MQ=60)
    TCATTTTTCGGAGACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAA                                                                                                                                                 <  SRR19973065.2477853/150‑1 (MQ=60)
        TTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAG                                                                                                                                             >  SRR19973065.1786328/1‑150 (MQ=60)
        TTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAG                                                                                                                                             <  SRR19973065.2039382/150‑1 (MQ=60)
        TTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAG                                                                                                                                             >  SRR19973065.211116/1‑150 (MQ=60)
           TCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCT                                                                                                                                          <  SRR19973065.398180/150‑1 (MQ=60)
              GATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGAT                                                                                                                                       >  SRR19973065.3126269/1‑150 (MQ=60)
              GATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGAT                                                                                                                                       <  SRR19973065.1559795/150‑1 (MQ=60)
               ATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATG                                                                                                                                      <  SRR19973065.953741/150‑1 (MQ=60)
                 ACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCT                                                                                                                                    <  SRR19973065.779957/150‑1 (MQ=60)
                  CCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTG                                                                                                                                   >  SRR19973065.3061687/1‑150 (MQ=60)
                  CCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTG                                                                                                                                   <  SRR19973065.1457891/150‑1 (MQ=60)
                  CCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTG                                                                                                                                   <  SRR19973065.509105/150‑1 (MQ=60)
                    AGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGAC                                                                                                                                 >  SRR19973065.3528989/1‑150 (MQ=60)
                     GGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACC                                                                                                                                >  SRR19973065.309904/1‑150 (MQ=60)
                     GGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACC                                                                                                                                >  SRR19973065.309903/1‑150 (MQ=60)
                     GGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACC                                                                                                                                >  SRR19973065.1199578/1‑150 (MQ=60)
                         GTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTT                                                                                                                            <  SRR19973065.2039470/150‑1 (MQ=60)
                           TGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTT                                                                                                                          <  SRR19973065.3175331/150‑1 (MQ=60)
                           TGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTT                                                                                                                          >  SRR19973065.2358761/1‑150 (MQ=60)
                                ACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCC                                                                                                                     >  SRR19973065.3440516/1‑150 (MQ=60)
                                 CTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCA                                                                                                                    >  SRR19973065.2159329/1‑150 (MQ=60)
                                  TCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAG                                                                                                                   <  SRR19973065.1684839/150‑1 (MQ=60)
                                  TCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAG                                                                                                                   <  SRR19973065.1685473/150‑1 (MQ=60)
                                    AGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTT                                                                                                                 >  SRR19973065.846364/1‑150 (MQ=60)
                                        CGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGT                                                                                                             >  SRR19973065.1867553/1‑150 (MQ=60)
                                          AATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGC                                                                                                           >  SRR19973065.999066/1‑150 (MQ=60)
                                                 TCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAAT                                                                                                    <  SRR19973065.239368/150‑1 (MQ=60)
                                                 TCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAAT                                                                                                    <  SRR19973065.3259877/150‑1 (MQ=60)
                                                 TCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAAT                                                                                                    <  SRR19973065.3378606/150‑1 (MQ=60)
                                                 TCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAAT                                                                                                    >  SRR19973065.1655743/1‑150 (MQ=60)
                                                  CTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATT                                                                                                   <  SRR19973065.2603562/150‑1 (MQ=60)
                                                  CTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATT                                                                                                   <  SRR19973065.2837692/150‑1 (MQ=60)
                                                  CTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATT                                                                                                   <  SRR19973065.2603569/150‑1 (MQ=60)
                                                   TATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTATGCCAGTTTTGTGCCTGTAATTC                                                                                                  >  SRR19973065.3461360/1‑150 (MQ=60)
                                                   TATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCACAACCAGTGAGCGAGTTGTTGCTAAGAAAAAGCCTGATGCTGACCATTTATCTGCCAGATTTGTGCATGTAATTC                                                                                                  >  SRR19973065.2311908/1‑150 (MQ=60)
                                                    ATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCT                                                                                                 >  SRR19973065.615936/1‑150 (MQ=60)
                                                    ATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCT                                                                                                 >  SRR19973065.616003/1‑150 (MQ=60)
                                                    ATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCT                                                                                                 <  SRR19973065.1541317/150‑1 (MQ=60)
                                                      TTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAA                                                                                               >  SRR19973065.3420097/1‑150 (MQ=60)
                                                      TTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAA                                                                                               >  SRR19973065.3418838/1‑150 (MQ=60)
                                                         AGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAAT                                                                                            <  SRR19973065.3206110/150‑1 (MQ=60)
                                                         AGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAAT                                                                                            <  SRR19973065.3206172/150‑1 (MQ=60)
                                                           GTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAG                                                                                          >  SRR19973065.3114025/1‑150 (MQ=60)
                                                           GTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAG                                                                                          >  SRR19973065.1330372/1‑150 (MQ=60)
                                                             TGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAT                                                                                        >  SRR19973065.3514595/1‑150 (MQ=60)
                                                              GGTTATGCAGAAAAGTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATT                                                                                       <  SRR19973065.2150715/150‑1 (MQ=60)
                                                               GTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTT                                                                                      >  SRR19973065.2223085/1‑150 (MQ=60)
                                                               GTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTT                                                                                      <  SRR19973065.1164299/150‑1 (MQ=60)
                                                                TTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTG                                                                                     <  SRR19973065.2154144/150‑1 (MQ=60)
                                                                  ATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTC                                                                                   >  SRR19973065.1347797/1‑150 (MQ=60)
                                                                     CAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTT                                                                                <  SRR19973065.1114155/150‑1 (MQ=60)
                                                                     CAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCCGTAATTCTAAAATAGATTTGTCTTT                                                                                <  SRR19973065.1622704/150‑1 (MQ=60)
                                                                     CAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTATGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTT                                                                                <  SRR19973065.1976268/150‑1 (MQ=60)
                                                                      AGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTA                                                                               >  SRR19973065.624732/1‑150 (MQ=60)
                                                                         AAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCC                                                                            >  SRR19973065.2956780/1‑150 (MQ=60)
                                                                         AAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCC                                                                            >  SRR19973065.2522428/1‑150 (MQ=60)
                                                                         AAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGGTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAATTAGATTTGTCTTTAGCG                                                                            >  SRR19973065.2522419/1‑150 (MQ=60)
                                                                           ATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTG                                                                          >  SRR19973065.2025260/1‑150 (MQ=60)
                                                                           ATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTG                                                                          <  SRR19973065.1000552/150‑1 (MQ=60)
                                                                           ATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTG                                                                          >  SRR19973065.242003/1‑150 (MQ=60)
                                                                           ATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCNG                                                                          <  SRR19973065.1000547/150‑1 (MQ=60)
                                                                           ATTGTAGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTG                                                                          >  SRR19973065.2025257/1‑150 (MQ=60)
                                                                             TGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAG                                                                        <  SRR19973065.1113364/150‑1 (MQ=60)
                                                                              GTCGGGATAATAGCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGG                                                                       >  SRR19973065.2605719/1‑150 (MQ=60)
                                                                               TCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGG                                                                      <  SRR19973065.2102407/150‑1 (MQ=60)
                                                                                 GGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGAT                                                                    <  SRR19973065.797326/150‑1 (MQ=60)
                                                                                  GGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATG                                                                   <  SRR19973065.1706949/150‑1 (MQ=60)
                                                                                  GGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATG                                                                   >  SRR19973065.970662/1‑150 (MQ=60)
                                                                                      AATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTT                                                               >  SRR19973065.43216/1‑150 (MQ=60)
                                                                                       ATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTNATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTT                                                              <  SRR19973065.1796251/150‑1 (MQ=60)
                                                                                       ATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTT                                                              <  SRR19973065.2300483/150‑1 (MQ=60)
                                                                                       ATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTT                                                              <  SRR19973065.883721/150‑1 (MQ=60)
                                                                                       ATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTT                                                              <  SRR19973065.2650770/150‑1 (MQ=60)
                                                                                       ATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTT                                                              <  SRR19973065.1089414/150‑1 (MQ=60)
                                                                                         AACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAA                                                            <  SRR19973065.3444595/150‑1 (MQ=60)
                                                                                           CCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATT                                                          >  SRR19973065.1642924/1‑150 (MQ=60)
                                                                                            CATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTT                                                         >  SRR19973065.796368/1‑150 (MQ=60)
                                                                                             ATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTG                                                        >  SRR19973065.1191245/1‑150 (MQ=60)
                                                                                             ATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTG                                                        >  SRR19973065.2951640/1‑150 (MQ=60)
                                                                                              TAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTGTTAATTTGA                                                       >  SRR19973065.1096781/1‑150 (MQ=60)
                                                                                               AGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAT                                                      >  SRR19973065.973586/1‑150 (MQ=60)
                                                                                               AGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAT                                                      >  SRR19973065.975979/1‑150 (MQ=60)
                                                                                               AGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAT                                                      >  SRR19973065.2355546/1‑150 (MQ=60)
                                                                                                GTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATT                                                     <  SRR19973065.1995433/150‑1 (MQ=60)
                                                                                                GTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATT                                                     <  SRR19973065.3367659/150‑1 (MQ=60)
                                                                                                GTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATT                                                     <  SRR19973065.210076/150‑1 (MQ=60)
                                                                                                 TTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTG                                                    >  SRR19973065.2579039/1‑150 (MQ=60)
                                                                                                 TTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTG                                                    <  SRR19973065.2462278/150‑1 (MQ=60)
                                                                                                  TTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGG                                                   <  SRR19973065.329821/150‑1 (MQ=60)
                                                                                                        CTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATT                                             >  SRR19973065.276440/1‑150 (MQ=60)
                                                                                                        CTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATT                                             >  SRR19973065.276158/1‑150 (MQ=60)
                                                                                                         TCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTG                                            <  SRR19973065.1509945/150‑1 (MQ=60)
                                                                                                         TCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTG                                            <  SRR19973065.1509951/150‑1 (MQ=60)
                                                                                                         TCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTG                                            <  SRR19973065.3259067/150‑1 (MQ=60)
                                                                                                         TCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTG                                            <  SRR19973065.1510168/150‑1 (MQ=60)
                                                                                                                AGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAAC                                     >  SRR19973065.3380614/1‑150 (MQ=60)
                                                                                                                AGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAAC                                     <  SRR19973065.1401557/150‑1 (MQ=60)
                                                                                                                AGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAAC                                     <  SRR19973065.2616224/150‑1 (MQ=60)
                                                                                                                 GTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACC                                    <  SRR19973065.2266363/150‑1 (MQ=60)
                                                                                                                 GTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACC                                    <  SRR19973065.2266387/150‑1 (MQ=60)
                                                                                                                   TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCCGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                                  >  SRR19973065.1982790/1‑150 (MQ=60)
                                                                                                                     TAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTG                                <  SRR19973065.1216866/150‑1 (MQ=60)
                                                                                                                      AATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGG                               <  SRR19973065.2013436/150‑1 (MQ=60)
                                                                                                                        TAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTC                             >  SRR19973065.482237/1‑150 (MQ=60)
                                                                                                                        TAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTC                             >  SRR19973065.481823/1‑150 (MQ=60)
                                                                                                                          GGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAG                           >  SRR19973065.2252015/1‑150 (MQ=60)
                                                                                                                          GGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAG                           >  SRR19973065.2251253/1‑150 (MQ=60)
                                                                                                                           GCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGC                          >  SRR19973065.2284202/1‑150 (MQ=60)
                                                                                                                           GCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGC                          >  SRR19973065.901497/1‑150 (MQ=60)
                                                                                                                            CTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCC                         <  SRR19973065.2229220/150‑1 (MQ=60)
                                                                                                                                TCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATT                     <  SRR19973065.2759428/150‑1 (MQ=60)
                                                                                                                                  CAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTT                   >  SRR19973065.1983187/1‑150 (MQ=60)
                                                                                                                                  CAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTT                   >  SRR19973065.1982855/1‑150 (MQ=60)
                                                                                                                                    GTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTC                 <  SRR19973065.831688/150‑1 (MQ=60)
                                                                                                                                          GAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGAC           >  SRR19973065.2499809/1‑150 (MQ=60)
                                                                                                                                           AGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACA          >  SRR19973065.2449753/1‑150 (MQ=60)
                                                                                                                                           AGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACA          <  SRR19973065.659635/150‑1 (MQ=60)
                                                                                                                                            GTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACAC         >  SRR19973065.1199664/1‑150 (MQ=60)
                                                                                                                                            GTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACAC         >  SRR19973065.1441763/1‑150 (MQ=60)
                                                                                                                                            GTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACAC         >  SRR19973065.1441931/1‑150 (MQ=60)
                                                                                                                                            GTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACAC         >  SRR19973065.509980/1‑150 (MQ=60)
                                                                                                                                            GTTGTTGCTAAGAAATAGCCTGATGATGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTTTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTAAGCCGATTTTTCTGCGACAC         >  SRR19973065.2287949/1‑150 (MQ=60)
                                                                                                                                               GTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT      >  SRR19973065.2903069/1‑150 (MQ=60)
                                                                                                                                               GTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT      >  SRR19973065.2821417/1‑150 (MQ=60)
                                                                                                                                               GTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT      >  SRR19973065.1918314/1‑150 (MQ=60)
                                                                                                                                               GTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTT      >  SRR19973065.2903163/1‑150 (MQ=60)
                                                                                                                                                 TGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCC    >  SRR19973065.2657539/1‑150 (MQ=60)
                                                                                                                                                   CTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAG  >  SRR19973065.32127/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TTAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCTTCCAG  >  CP009273/1083295‑1083591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑