Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,083,443 G→T 100% S624* (TCG→TAG)  pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,083,4430GT98.6% 687.5 / ‑0.2 210S624* (TCG→TAG) pgaBpoly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (113/94);  total (115/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

TAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCT  >  CP009273/1083296‑1083586
                                                                                                                                                   |                                                                                                                                               
tAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaa                                                                                                                                               >  1:2472860/1‑150 (MQ=255)
 aaTCATTTTTCGGCTACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTGGGTGATGCAGAAAATTGTCGGGCTAATAACCATAGTTTTTCACTCCATTCAGTTGTACTAGGCTCATCCAGTGAGCGAGTTGTTGCtaag                                                                                                                                              >  1:2358805/1‑150 (MQ=255)
 aaTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaag                                                                                                                                              >  1:3706856/1‑150 (MQ=255)
  aTCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCtaaga                                                                                                                                             >  2:1667108/1‑150 (MQ=255)
          tCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCt                                                                                                                                     <  2:3878308/150‑1 (MQ=255)
           cGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTg                                                                                                                                    >  1:2115105/1‑150 (MQ=255)
            ggATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGa                                                                                                                                   >  1:2286761/1‑150 (MQ=255)
             gATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGAt                                                                                                                                  >  1:3905761/1‑150 (MQ=255)
              agaCCAGGCTGTCGACAACTCAGGACGACTAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATg                                                                                                                                 <  2:4327953/148‑1 (MQ=255)
              aTACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATg                                                                                                                                 <  2:4830900/150‑1 (MQ=255)
               tACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCAGACAGTGAGCGAGTTGTTGCTATGAAATAGCCTGTTgg                                                                                                                                >  2:4806130/1‑149 (MQ=255)
                aCCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCt                                                                                                                               <  1:2020841/150‑1 (MQ=255)
                aCCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCt                                                                                                                               <  1:1385557/150‑1 (MQ=255)
                 ccAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCAAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTg                                                                                                                              >  1:3665705/1‑150 (MQ=255)
                 ccAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATACAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATgaag                                                                                                                              >  2:1593165/1‑147 (MQ=255)
                  cAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGa                                                                                                                             >  2:4105586/1‑150 (MQ=255)
                    ggCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGAcc                                                                                                                           <  1:4042556/150‑1 (MQ=255)
                    ggCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGAcc                                                                                                                           >  2:542742/1‑150 (MQ=255)
                     gCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCa                                                                                                                          >  2:1181969/1‑150 (MQ=255)
                        gTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCAttt                                                                                                                       <  1:149128/150‑1 (MQ=255)
                            aaaaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCt                                                                                                                   >  2:1878843/1‑150 (MQ=255)
                              aaCTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGc                                                                                                                 >  2:4645083/1‑150 (MQ=255)
                                  cAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGt                                                                                                             <  2:4761393/150‑1 (MQ=255)
                                  cAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCAAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGt                                                                                                             >  1:1788387/1‑150 (MQ=255)
                                   aGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGtt                                                                                                            <  2:1137319/150‑1 (MQ=255)
                                     gACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGtttt                                                                                                          <  2:2247675/150‑1 (MQ=255)
                                     gACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTGTCTGCCAGtttt                                                                                                          >  2:2996545/1‑150 (MQ=255)
                                      aCGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTtg                                                                                                         <  2:4879397/150‑1 (MQ=255)
                                       cGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTtgt                                                                                                        <  2:5158355/150‑1 (MQ=255)
                                        gAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTtgtg                                                                                                       <  2:4556151/150‑1 (MQ=255)
                                         aaTAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGc                                                                                                      <  2:7954/150‑1 (MQ=255)
                                         aaTAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGc                                                                                                      <  2:784968/150‑1 (MQ=255)
                                            aaGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTg                                                                                                   >  1:485610/1‑150 (MQ=255)
                                               gTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTaa                                                                                                <  1:307500/150‑1 (MQ=255)
                                                tCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAAt                                                                                               <  1:3227112/150‑1 (MQ=255)
                                                  tATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCAAGTTTTGTGCCTGTAATTc                                                                                             >  2:2721076/1‑150 (MQ=255)
                                                   ttttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCt                                                                                            >  1:1137734/2‑150 (MQ=255)
                                                    tttCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTa                                                                                           >  2:751564/1‑150 (MQ=255)
                                                      tCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTaaa                                                                                         <  1:3964171/150‑1 (MQ=255)
                                                           ttggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGa                                                                                    >  2:3680980/1‑150 (MQ=255)
                                                            tggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAt                                                                                   >  2:1264968/1‑150 (MQ=255)
                                                             ggttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAtt                                                                                  >  1:4509215/1‑150 (MQ=255)
                                                              gttATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGAttt                                                                                 >  1:1238284/1‑150 (MQ=255)
                                                                 aTGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTc                                                                              >  1:4067790/1‑150 (MQ=255)
                                                                   gCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCtt                                                                            >  1:2068980/1‑150 (MQ=255)
                                                                   gCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCtt                                                                            >  2:4416883/1‑150 (MQ=255)
                                                                    cAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCttt                                                                           <  1:3330949/150‑1 (MQ=255)
                                                                    cAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCttt                                                                           <  1:5069013/150‑1 (MQ=255)
                                                                    cAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCttt                                                                           >  2:4655453/1‑150 (MQ=255)
                                                                      gAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAg                                                                         <  1:2727013/150‑1 (MQ=255)
                                                                      gAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAg                                                                         <  2:4675201/150‑1 (MQ=255)
                                                                       aaaaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTTTAATAGTCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTTATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGc                                                                        <  2:2358805/150‑1 (MQ=255)
                                                                       aaaaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGc                                                                        >  1:1429325/1‑150 (MQ=255)
                                                                        aaaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGcc                                                                       <  2:3816329/150‑1 (MQ=255)
                                                                        aaaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGcc                                                                       >  2:394214/1‑150 (MQ=255)
                                                                         aaTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCt                                                                      >  2:3306268/1‑150 (MQ=255)
                                                                          aTTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTg                                                                     >  2:3151824/1‑150 (MQ=255)
                                                                                 ggATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATg                                                              <  2:275694/150‑1 (MQ=255)
                                                                                   ataataACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtt                                                            >  1:75892/1‑150 (MQ=255)
                                                                                     aataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtttt                                                          >  1:4005615/1‑150 (MQ=255)
                                                                                     aataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtttt                                                          <  2:490547/150‑1 (MQ=255)
                                                                                     aataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGtttt                                                          >  1:4757851/1‑150 (MQ=255)
                                                                                      ataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttttt                                                         >  2:3497510/1‑150 (MQ=255)
                                                                                      ataaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGttttt                                                         <  1:3416633/150‑1 (MQ=255)
                                                                                       taaCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTa                                                        <  1:4205664/150‑1 (MQ=255)
                                                                                         aCCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAt                                                      <  2:1864146/150‑1 (MQ=255)
                                                                                          ccATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAAtt                                                     <  1:1275038/150‑1 (MQ=255)
                                                                                          acATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGACTGTAATTCTAAAATATATTTGTCTTTAGCCTGAGGGATGTTTTTAAtt                                                     >  2:1342416/2‑150 (MQ=255)
                                                                                            aTAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTg                                                   <  1:622761/150‑1 (MQ=255)
                                                                                              aGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAt                                                 >  1:3834788/1‑150 (MQ=255)
                                                                                                 ttttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTgg                                              >  2:3563790/1‑150 (MQ=255)
                                                                                                   ttCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTc                                            >  1:1838024/1‑150 (MQ=255)
                                                                                                     cACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCaa                                          >  2:4068307/1‑150 (MQ=255)
                                                                                                       cTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAtt                                        >  2:2383396/1‑150 (MQ=255)
                                                                                                       cTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGATTTGTGACTGTAATTCTAAAATAGATTTGTCTATAGCCTGAGGGATGTTTTTAATTTGATTGGTCAAtt                                        >  2:2020173/1‑150 (MQ=255)
                                                                                                          cATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTa                                     <  1:4887257/150‑1 (MQ=255)
                                                                                                             tCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTa                                  >  1:3503466/1‑150 (MQ=255)
                                                                                                               aGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAAc                                >  2:257587/1‑150 (MQ=255)
                                                                                                                  tGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                             >  1:2645/1‑150 (MQ=255)
                                                                                                                  tGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                             >  1:2516085/1‑150 (MQ=255)
                                                                                                                  tGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAt                             <  1:970339/150‑1 (MQ=255)
                                                                                                                    tAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTg                           <  2:4894121/150‑1 (MQ=255)
                                                                                                                    tAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTg                           >  1:1926846/1‑150 (MQ=255)
                                                                                                                      aTAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGt                         >  2:3282114/1‑150 (MQ=255)
                                                                                                                      aTAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTGTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGt                         >  1:3872248/1‑150 (MQ=255)
                                                                                                                       tAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTc                        <  2:3750923/150‑1 (MQ=255)
                                                                                                                        aGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCa                       <  1:4330359/150‑1 (MQ=255)
                                                                                                                        aGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCa                       >  1:4575402/1‑150 (MQ=255)
                                                                                                                        aGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCa                       >  1:3116042/1‑150 (MQ=255)
                                                                                                                         ggCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAg                      <  1:1045088/150‑1 (MQ=255)
                                                                                                                             cATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGa                  <  2:2887245/150‑1 (MQ=255)
                                                                                                                              aTCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAt                 >  1:3199295/1‑150 (MQ=255)
                                                                                                                              aTCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAt                 >  1:4946647/1‑150 (MQ=255)
                                                                                                                               tCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAtt                <  2:2101937/150‑1 (MQ=255)
                                                                                                                                 cAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAtttt              >  1:5049213/1‑150 (MQ=255)
                                                                                                                                   gTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTc            >  1:4394072/1‑150 (MQ=255)
                                                                                                                                     gAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTg          <  1:1181969/150‑1 (MQ=255)
                                                                                                                                      aGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGc         <  2:1429325/150‑1 (MQ=255)
                                                                                                                                         gAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGACTGGTCAATTGTATTAACCATTGGTCCGCCGATTTTTCTGCGac      >  2:1943186/1‑150 (MQ=255)
                                                                                                                                             tgttgCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCt  >  1:1705123/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                               
TAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCT  >  CP009273/1083296‑1083586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCT  >  CP009273/1083296‑1083586
                                                                                                                                                   |                                                                                                                                               
TAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAA                                                                                                                                               >  SRR19973063.2480763/1‑150 (MQ=60)
 AATCATTTTTCGGCTACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTGGGTGATGCAGAAAATTGTCGGGCTAATAACCATAGTTTTTCACTCCATTCAGTTGTACTAGGCTCATCCAGTGAGCGAGTTGTTGCTAAG                                                                                                                                              >  SRR19973063.2366355/1‑150 (MQ=60)
 AATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAG                                                                                                                                              >  SRR19973063.2126283/1‑150 (MQ=60)
 AATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAG                                                                                                                                              >  SRR19973063.3718711/1‑150 (MQ=60)
       TTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAG                                                                                                                                        >  SRR19973063.491951/1‑150 (MQ=60)
          TCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCT                                                                                                                                     >  SRR19973063.1929135/1‑150 (MQ=60)
           CGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTG                                                                                                                                    >  SRR19973063.4376877/1‑150 (MQ=60)
           CGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTG                                                                                                                                    >  SRR19973063.2121725/1‑150 (MQ=60)
            GGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGA                                                                                                                                   >  SRR19973063.2294058/1‑150 (MQ=60)
             GATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGAT                                                                                                                                  >  SRR19973063.3918360/1‑150 (MQ=60)
             GATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGAT                                                                                                                                  >  SRR19973063.3918208/1‑150 (MQ=60)
                ACCAGGCTGTTGAAAACTCGGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCT                                                                                                                               <  SRR19973063.1439618/150‑1 (MQ=60)
                ACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCT                                                                                                                               <  SRR19973063.2027147/150‑1 (MQ=60)
                ACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCT                                                                                                                               <  SRR19973063.1389746/150‑1 (MQ=60)
                 CCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTG                                                                                                                              >  SRR19973063.3677466/1‑150 (MQ=60)
                 CCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCAAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTG                                                                                                                              >  SRR19973063.3677452/1‑150 (MQ=60)
                  CAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGA                                                                                                                             >  SRR19973063.2505600/1‑150 (MQ=60)
                    GGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACC                                                                                                                           <  SRR19973063.4055510/150‑1 (MQ=60)
                    GGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACC                                                                                                                           <  SRR19973063.4062534/150‑1 (MQ=60)
                       TGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATT                                                                                                                        <  SRR19973063.1091072/150‑1 (MQ=60)
                        GTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTT                                                                                                                       <  SRR19973063.149589/150‑1 (MQ=60)
                           GAAAACTCAGGACGAATAAGGTCTATTTCAGGTAGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTC                                                                                                                    >  SRR19973063.3828478/1‑150 (MQ=60)
                            AAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCT                                                                                                                   >  SRR19973063.2108512/1‑150 (MQ=60)
                             AAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGTGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTG                                                                                                                  <  SRR19973063.524305/150‑1 (MQ=60)
                              AACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGC                                                                                                                 >  SRR19973063.3466728/1‑150 (MQ=60)
                              AACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGC                                                                                                                 >  SRR19973063.3984893/1‑150 (MQ=60)
                               ACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCC                                                                                                                <  SRR19973063.4395172/150‑1 (MQ=60)
                                CTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCA                                                                                                               <  SRR19973063.3909719/150‑1 (MQ=60)
                                  CAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGT                                                                                                             >  SRR19973063.1793968/1‑150 (MQ=60)
                                  CAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGT                                                                                                             >  SRR19973063.1793810/1‑150 (MQ=60)
                                  CAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCAAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGT                                                                                                             >  SRR19973063.1793916/1‑150 (MQ=60)
                                   AGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTT                                                                                                            >  SRR19973063.3994710/1‑150 (MQ=60)
                                      ACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTG                                                                                                         <  SRR19973063.1251992/150‑1 (MQ=60)
                                         AATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTGTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGC                                                                                                      >  SRR19973063.3884389/1‑150 (MQ=60)
                                            AAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTG                                                                                                   >  SRR19973063.486995/1‑150 (MQ=60)
                                               GTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAA                                                                                                <  SRR19973063.308403/150‑1 (MQ=60)
                                                TCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAAT                                                                                               <  SRR19973063.3237482/150‑1 (MQ=60)
                                                   TTTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCT                                                                                            >  SRR19973063.1141179/1‑150 (MQ=60)
                                                     TTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAA                                                                                          <  SRR19973063.4805589/150‑1 (MQ=60)
                                                      TCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAA                                                                                         <  SRR19973063.3976856/150‑1 (MQ=60)
                                                      TCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAA                                                                                         >  SRR19973063.735861/1‑150 (MQ=60)
                                                        AGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAAT                                                                                       >  SRR19973063.1406142/1‑150 (MQ=60)
                                                        AGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAAGAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAAT                                                                                       <  SRR19973063.2061668/150‑1 (MQ=60)
                                                         GGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATA                                                                                      >  SRR19973063.4531316/1‑150 (MQ=60)
                                                         GGTTGGTAATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATA                                                                                      >  SRR19973063.3228705/1‑150 (MQ=60)
                                                             GGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATT                                                                                  >  SRR19973063.4255721/1‑150 (MQ=60)
                                                             GGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATT                                                                                  >  SRR19973063.4523685/1‑150 (MQ=60)
                                                             GGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATT                                                                                  >  SRR19973063.828747/1‑150 (MQ=60)
                                                              GTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTT                                                                                 >  SRR19973063.450243/1‑150 (MQ=60)
                                                              GTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTT                                                                                 >  SRR19973063.1241980/1‑150 (MQ=60)
                                                                 ATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTC                                                                              >  SRR19973063.4080828/1‑150 (MQ=60)
                                                                  TGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCT                                                                             <  SRR19973063.2879604/150‑1 (MQ=60)
                                                                   GCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTT                                                                            >  SRR19973063.2075447/1‑150 (MQ=60)
                                                                    CAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCCGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTT                                                                           >  SRR19973063.3262917/1‑150 (MQ=60)
                                                                    CAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCATTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTT                                                                           <  SRR19973063.1183287/150‑1 (MQ=60)
                                                                    CAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTT                                                                           <  SRR19973063.3341578/150‑1 (MQ=60)
                                                                    CAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTT                                                                           <  SRR19973063.5085320/150‑1 (MQ=60)
                                                                      GAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAG                                                                         <  SRR19973063.2735816/150‑1 (MQ=60)
                                                                       AAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGC                                                                        >  SRR19973063.1433682/1‑150 (MQ=60)
                                                                       AAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTATTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGC                                                                        >  SRR19973063.4047672/1‑150 (MQ=60)
                                                                             GTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGG                                                                  >  SRR19973063.4874004/1‑150 (MQ=60)
                                                                              TCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGG                                                                 >  SRR19973063.1219194/1‑150 (MQ=60)
                                                                                GGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGAT                                                               >  SRR19973063.1346072/1‑150 (MQ=60)
                                                                                 GGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATG                                                              <  SRR19973063.4188038/150‑1 (MQ=60)
                                                                                 GGATAATAACCACAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATG                                                              <  SRR19973063.3143055/150‑1 (MQ=60)
                                                                                   ATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTT                                                            >  SRR19973063.76155/1‑150 (MQ=60)
                                                                                   ATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTT                                                            <  SRR19973063.5003924/150‑1 (MQ=60)
                                                                                     AATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTT                                                          >  SRR19973063.4773024/1‑150 (MQ=60)
                                                                                     AATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTT                                                          >  SRR19973063.4018463/1‑150 (MQ=60)
                                                                                      ATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTT                                                         <  SRR19973063.3427509/150‑1 (MQ=60)
                                                                                       TAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTA                                                        <  SRR19973063.4219081/150‑1 (MQ=60)
                                                                                          CCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATT                                                     <  SRR19973063.1278834/150‑1 (MQ=60)
                                                                                            ATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTG                                                   <  SRR19973063.624592/150‑1 (MQ=60)
                                                                                              AGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGAT                                                 >  SRR19973063.3846985/1‑150 (MQ=60)
                                                                                                TTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTG                                               <  SRR19973063.3527548/150‑1 (MQ=60)
                                                                                                   TTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTC                                            >  SRR19973063.1843759/1‑150 (MQ=60)
                                                                                                         CCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGT                                      >  SRR19973063.1342810/1‑150 (MQ=60)
                                                                                                         CCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGT                                      >  SRR19973063.3142088/1‑150 (MQ=60)
                                                                                                         CCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGT                                      >  SRR19973063.880168/1‑150 (MQ=60)
                                                                                                          CATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTA                                     <  SRR19973063.4903023/150‑1 (MQ=60)
                                                                                                          CATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTA                                     <  SRR19973063.2334687/150‑1 (MQ=60)
                                                                                                             TCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTA                                  >  SRR19973063.3514668/1‑150 (MQ=60)
                                                                                                              CAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAA                                 <  SRR19973063.266416/150‑1 (MQ=60)
                                                                                                                GTTGTAATAGGCTCATCCAGTGAGCGAGTTTTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACC                               <  SRR19973063.3347668/150‑1 (MQ=60)
                                                                                                                  TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                             <  SRR19973063.973262/150‑1 (MQ=60)
                                                                                                                  TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                             >  SRR19973063.2656/1‑150 (MQ=60)
                                                                                                                  TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                             >  SRR19973063.2406/1‑150 (MQ=60)
                                                                                                                  TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                             >  SRR19973063.2408/1‑150 (MQ=60)
                                                                                                                  TGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCAT                             >  SRR19973063.2524108/1‑150 (MQ=60)
                                                                                                                    TAATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTG                           >  SRR19973063.1932870/1‑150 (MQ=60)
                                                                                                                     AATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGG                          <  SRR19973063.353522/150‑1 (MQ=60)
                                                                                                                      ATAGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTGTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGT                         >  SRR19973063.3884556/1‑150 (MQ=60)
                                                                                                                        AGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCA                       >  SRR19973063.4590037/1‑150 (MQ=60)
                                                                                                                        AGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCA                       <  SRR19973063.4344157/150‑1 (MQ=60)
                                                                                                                        AGGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCA                       >  SRR19973063.1423982/1‑150 (MQ=60)
                                                                                                                        AGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCA                       >  SRR19973063.3126072/1‑150 (MQ=60)
                                                                                                                         GGCTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAG                      <  SRR19973063.1048253/150‑1 (MQ=60)
                                                                                                                           CTCATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCTTTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCC                    <  SRR19973063.2562258/150‑1 (MQ=60)
                                                                                                                              ATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAT                 >  SRR19973063.4962025/1‑150 (MQ=60)
                                                                                                                              ATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAT                 >  SRR19973063.3209580/1‑150 (MQ=60)
                                                                                                                              ATCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGAT                 >  SRR19973063.4962646/1‑150 (MQ=60)
                                                                                                                               TCCAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATT                >  SRR19973063.3124598/1‑150 (MQ=60)
                                                                                                                                 CAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTT              >  SRR19973063.5065477/1‑150 (MQ=60)
                                                                                                                                 CAGTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTT              >  SRR19973063.5065503/1‑150 (MQ=60)
                                                                                                                                   GTGAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTC            >  SRR19973063.4408147/1‑150 (MQ=60)
                                                                                                                                     GAGCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTG          <  SRR19973063.1185534/150‑1 (MQ=60)
                                                                                                                                       GCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCG        <  SRR19973063.1943326/150‑1 (MQ=60)
                                                                                                                                       GCGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCG        <  SRR19973063.1943020/150‑1 (MQ=60)
                                                                                                                                        CGAGTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGA       >  SRR19973063.162213/1‑150 (MQ=60)
                                                                                                                                           GTTGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAACCGATTTTTCTGCGACAC    >  SRR19973063.4729938/1‑150 (MQ=60)
                                                                                                                                             TGTTGCTAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCT  >  SRR19973063.1710320/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                               
TAATCATTTTTCGGATACCAGGCTGTTGAAAACTCAGGACGAATAAGGTCTATTTCAGGTTGGTTATGCAGAAAATTGTCGGGATAATAACCATAGTTTTTCACTCCATTCAGTTGTAATAGGCTCATCCAGTGAGCGAGTTGTTGCGAAGAAATAGCCTGATGCTGACCATTTTTCTGCCAGTTTTGTGCCTGTAATTCTAAAATAGATTTGTCTTTAGCCTGAGGGATGTTTTTAATTTGATTGGTCAATTGTATTAACCATTGGTCAGCCGATTTTTCTGCGACACCT  >  CP009273/1083296‑1083586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑