Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 253,855 G→T 100% R281L (CGT→CTT)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273253,8550GT100.0% 644.4 / NA 195R281L (CGT→CTT) frsAfermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (75/120);  total (75/120)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 195 total aligned reads displayed.

TGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGA  >  CP009273/253711‑253999
                                                                                                                                                |                                                                                                                                                
 gggCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTg                                                                                                                                              >  1:3346701/1‑148 (MQ=255)
       ttcttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATAc                                                                                                                                        >  2:791807/1‑148 (MQ=255)
       ttcttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATAc                                                                                                                                        >  2:1253363/1‑148 (MQ=255)
         cttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCt                                                                                                                                      >  1:1745170/1‑148 (MQ=255)
           tcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTg                                                                                                                                    >  1:182655/1‑148 (MQ=255)
           tcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTg                                                                                                                                    <  1:3009125/148‑1 (MQ=255)
            cAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCTGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGa                                                                                                                                   <  2:1744246/148‑1 (MQ=255)
            cAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGa                                                                                                                                   <  2:2474694/148‑1 (MQ=255)
              aaaTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAt                                                                                                                                 <  1:1938496/148‑1 (MQ=255)
              aaaTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAt                                                                                                                                 <  2:2187186/148‑1 (MQ=255)
              aaaTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAt                                                                                                                                 <  2:3067947/148‑1 (MQ=255)
                  ggAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcgcc                                                                                                                             >  1:1533207/1‑148 (MQ=255)
                      gCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGt                                                                                                                         <  1:2316844/148‑1 (MQ=255)
                      gCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGt                                                                                                                         <  1:2316794/148‑1 (MQ=255)
                        tCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCt                                                                                                                       <  1:3396044/148‑1 (MQ=255)
                            ccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGaaa                                                                                                                   <  1:560575/148‑1 (MQ=255)
                              aGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGc                                                                                                                 >  2:3481559/1‑148 (MQ=255)
                               ggACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCg                                                                                                                >  1:2731986/1‑148 (MQ=255)
                                gACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCgg                                                                                                               >  1:3088519/1‑148 (MQ=255)
                                gACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCgg                                                                                                               >  1:2044515/1‑148 (MQ=255)
                                 aCTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGt                                                                                                              >  1:1288357/1‑148 (MQ=255)
                                 aCTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGt                                                                                                              <  1:1960397/148‑1 (MQ=255)
                                 aCTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGt                                                                                                              >  1:1075641/1‑148 (MQ=255)
                                 aCTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGt                                                                                                              >  1:2678150/1‑148 (MQ=255)
                                   tCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTg                                                                                                            <  2:1990649/148‑1 (MQ=255)
                                    ccAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGc                                                                                                           <  2:1967535/148‑1 (MQ=255)
                                      aGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCt                                                                                                         >  2:3392002/1‑148 (MQ=255)
                                      aGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCt                                                                                                         >  2:2075375/1‑148 (MQ=255)
                                       gCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTg                                                                                                        >  1:2365274/1‑148 (MQ=255)
                                        ccTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTGGCCTGt                                                                                                       >  2:2463772/1‑148 (MQ=255)
                                         cTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTc                                                                                                      <  2:1446512/148‑1 (MQ=255)
                                          tGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCt                                                                                                     <  1:2727679/148‑1 (MQ=255)
                                           gTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCtt                                                                                                    <  1:993772/148‑1 (MQ=255)
                                            ttGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                   >  1:766240/1‑148 (MQ=255)
                                            ttGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                   <  2:1917933/148‑1 (MQ=255)
                                                aTCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTcc                                                                                               >  1:1844899/1‑148 (MQ=255)
                                                 tCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCg                                                                                              <  1:156347/148‑1 (MQ=255)
                                                     cACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAg                                                                                          >  1:1729261/1‑148 (MQ=255)
                                                        gTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTc                                                                                       <  1:2465144/148‑1 (MQ=255)
                                                         tCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCa                                                                                      <  1:210138/148‑1 (MQ=255)
                                                           ttAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATa                                                                                    <  1:364769/148‑1 (MQ=255)
                                                            tAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAc                                                                                   <  1:2869360/148‑1 (MQ=255)
                                                                ggCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                               <  1:898273/148‑1 (MQ=255)
                                                                ggCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                               <  1:1471624/148‑1 (MQ=255)
                                                                ggCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                               <  2:703064/148‑1 (MQ=255)
                                                                ggCGCTGCCGAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                               <  2:421079/148‑1 (MQ=255)
                                                                 gcgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGt                                                                              >  1:481436/1‑148 (MQ=255)
                                                                 gcgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGt                                                                              <  1:2997723/148‑1 (MQ=255)
                                                                 gcgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGt                                                                              <  1:1736447/148‑1 (MQ=255)
                                                                  cgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGtt                                                                             <  1:1670249/148‑1 (MQ=255)
                                                                  cgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCAGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGtt                                                                             <  1:2391314/148‑1 (MQ=255)
                                                                   gcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTg                                                                            <  1:1453299/148‑1 (MQ=255)
                                                                    cTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGa                                                                           <  1:1045943/148‑1 (MQ=255)
                                                                    cTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGa                                                                           <  2:1347086/148‑1 (MQ=255)
                                                                    cTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGa                                                                           <  1:1046355/148‑1 (MQ=255)
                                                                       ccTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTg                                                                        <  2:2210728/148‑1 (MQ=255)
                                                                       ccTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTg                                                                        >  2:2604667/1‑148 (MQ=255)
                                                                          aaCGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtt                                                                     <  2:683145/148‑1 (MQ=255)
                                                                           aCGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAttt                                                                    >  2:2935682/1‑148 (MQ=255)
                                                                             gTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTa                                                                  <  2:1637121/148‑1 (MQ=255)
                                                                                 cGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTg                                                              >  2:2434068/1‑148 (MQ=255)
                                                                                  gtgggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGc                                                             <  1:3238283/148‑1 (MQ=255)
                                                                                   tgggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGcc                                                            <  2:1462527/148‑1 (MQ=255)
                                                                                       tggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCa                                                        <  1:1673200/148‑1 (MQ=255)
                                                                                          aTCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCGGTTGAGTGATTTTAAGTGCCAGCAACa                                                     >  2:888501/1‑148 (MQ=255)
                                                                                            cacaCTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAgg                                                   <  1:47512/148‑1 (MQ=255)
                                                                                              cacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTg                                                 <  2:2688815/148‑1 (MQ=255)
                                                                                              cacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTg                                                 <  2:934191/148‑1 (MQ=255)
                                                                                              cacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTg                                                 <  2:1832313/148‑1 (MQ=255)
                                                                                               acTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGc                                                <  2:2365274/148‑1 (MQ=255)
                                                                                                cTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGcc                                               >  2:272624/1‑148 (MQ=255)
                                                                                                  cgcgtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCgg                                             >  2:1204794/1‑148 (MQ=255)
                                                                                                     gtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGaaa                                          <  1:1692522/148‑1 (MQ=255)
                                                                                                      tcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAt                                         >  1:889125/1‑148 (MQ=255)
                                                                                                       cgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATg                                        >  2:2333021/1‑148 (MQ=255)
                                                                                                          ggCCTTTGGTTTCCGGTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTAt                                     <  2:2820500/148‑1 (MQ=255)
                                                                                                           gCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATc                                    <  2:576172/148‑1 (MQ=255)
                                                                                                            ccTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCt                                   <  2:1254828/148‑1 (MQ=255)
                                                                                                              tttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                 <  1:3368444/148‑1 (MQ=255)
                                                                                                                tGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAc                               >  1:2504678/1‑148 (MQ=255)
                                                                                                                 ggTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACg                              <  1:1939066/148‑1 (MQ=255)
                                                                                                                 ggTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACg                              <  1:979141/148‑1 (MQ=255)
                                                                                                                 ggTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACg                              <  1:1208859/148‑1 (MQ=255)
                                                                                                                   tttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGtt                            <  2:1464152/148‑1 (MQ=255)
                                                                                                                    ttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTc                           <  2:2452590/148‑1 (MQ=255)
                                                                                                                    ttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTATGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAACTGTATCTTGACGTTc                           >  2:226272/1‑148 (MQ=255)
                                                                                                                     tCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCt                          >  2:1346053/1‑148 (MQ=255)
                                                                                                                     tCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCt                          >  2:2854866/1‑148 (MQ=255)
                                                                                                                     tCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCt                          >  2:1810277/1‑148 (MQ=255)
                                                                                                                       cGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTgg                        <  1:1384704/148‑1 (MQ=255)
                                                                                                                              gcgcTAACGTTGCCGTGCTTCTGGCATACCTTGACTCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCg                 <  2:2706891/148‑1 (MQ=255)
                                                                                                                              gcgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCg                 <  2:1533207/148‑1 (MQ=255)
                                                                                                                                 cTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGttt              >  1:2527955/1‑148 (MQ=255)
                                                                                                                                 cTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGttt              <  1:2036395/148‑1 (MQ=255)
                                                                                                                                 cTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGttt              <  1:2036351/148‑1 (MQ=255)
                                                                                                                                 cTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGttt              <  2:1723120/148‑1 (MQ=255)
                                                                                                                                     cGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTgggg          <  1:1191279/148‑1 (MQ=255)
                                                                                                                                     cGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTgggg          <  2:182655/148‑1 (MQ=255)
                                                                                                                                        tGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATg       >  1:3365563/1‑148 (MQ=255)
                                                                                                                                             tGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTGTGGGGATGCatga  >  2:374314/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGA  >  CP009273/253711‑253999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  >  CP009273/253709‑254004
                                                                                                                                                  |                                                                                                                                                     
GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCT                                                                                                                                                    >  SRR19973065.1980372/1‑150 (MQ=60)
 GTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTG                                                                                                                                                   >  SRR19973065.3367150/1‑150 (MQ=60)
    GGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCA                                                                                                                                                <  SRR19973065.339074/150‑1 (MQ=60)
       TTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATAC                                                                                                                                             >  SRR19973065.1224133/1‑150 (MQ=60)
         TTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCT                                                                                                                                           >  SRR19973065.1756680/1‑150 (MQ=60)
         TTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCT                                                                                                                                           >  SRR19973065.3502518/1‑150 (MQ=60)
           CTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTG                                                                                                                                         >  SRR19973065.184099/1‑150 (MQ=60)
           CTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTG                                                                                                                                         >  SRR19973065.184097/1‑150 (MQ=60)
             TCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAA                                                                                                                                       <  SRR19973065.3027550/150‑1 (MQ=60)
                AAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCG                                                                                                                                    <  SRR19973065.1951179/150‑1 (MQ=60)
                AAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCG                                                                                                                                    <  SRR19973065.1951298/150‑1 (MQ=60)
                AAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCG                                                                                                                                    >  SRR19973065.2352335/1‑150 (MQ=60)
                  ATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCC                                                                                                                                  >  SRR19973065.1543316/1‑150 (MQ=60)
                        GCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCT                                                                                                                            <  SRR19973065.2331369/150‑1 (MQ=60)
                        GCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCT                                                                                                                            <  SRR19973065.2331340/150‑1 (MQ=60)
                        GCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCT                                                                                                                            <  SRR19973065.2331319/150‑1 (MQ=60)
                        GCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCT                                                                                                                            <  SRR19973065.557216/150‑1 (MQ=60)
                          TCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGA                                                                                                                          <  SRR19973065.3416808/150‑1 (MQ=60)
                              CCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGC                                                                                                                      <  SRR19973065.564143/150‑1 (MQ=60)
                               CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                     <  SRR19973065.3018785/150‑1 (MQ=60)
                               CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                     >  SRR19973065.2748983/1‑150 (MQ=60)
                                AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGG                                                                                                                    >  SRR19973065.2057599/1‑150 (MQ=60)
                                AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGG                                                                                                                    >  SRR19973065.2057554/1‑150 (MQ=60)
                                AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGG                                                                                                                    >  SRR19973065.3107380/1‑150 (MQ=60)
                                 GGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGT                                                                                                                   >  SRR19973065.2694753/1‑150 (MQ=60)
                                 GGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGT                                                                                                                   >  SRR19973065.1082754/1‑150 (MQ=60)
                                 GGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGT                                                                                                                   >  SRR19973065.1296852/1‑150 (MQ=60)
                                  GACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTT                                                                                                                  <  SRR19973065.643015/150‑1 (MQ=60)
                                  GACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTT                                                                                                                  <  SRR19973065.274395/150‑1 (MQ=60)
                                   ACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTG                                                                                                                 <  SRR19973065.1973200/150‑1 (MQ=60)
                                       CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             >  SRR19973065.1840775/1‑150 (MQ=60)
                                       CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             >  SRR19973065.2380007/1‑150 (MQ=60)
                                        AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTAGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGT                                                                                                            >  SRR19973065.3385598/1‑150 (MQ=60)
                                           CTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTT                                                                                                         <  SRR19973065.1207751/150‑1 (MQ=60)
                                            TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                        <  SRR19973065.2646512/150‑1 (MQ=60)
                                            TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                        <  SRR19973065.2744645/150‑1 (MQ=60)
                                            TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                        <  SRR19973065.2745643/150‑1 (MQ=60)
                                            TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                        >  SRR19973065.2540997/1‑150 (MQ=60)
                                            TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                        >  SRR19973065.771330/1‑150 (MQ=60)
                                             GTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGG                                                                                                       <  SRR19973065.1000379/150‑1 (MQ=60)
                                             GTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGG                                                                                                       >  SRR19973065.2124695/1‑150 (MQ=60)
                                                GCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCC                                                                                                    >  SRR19973065.1857063/1‑150 (MQ=60)
                                                   TCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGT                                                                                                 <  SRR19973065.902177/150‑1 (MQ=60)
                                                   TCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGT                                                                                                 <  SRR19973065.157644/150‑1 (MQ=60)
                                                    CAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTA                                                                                                <  SRR19973065.484191/150‑1 (MQ=60)
                                                    CAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCAGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTA                                                                                                >  SRR19973065.2468046/1‑150 (MQ=60)
                                                     AGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAG                                                                                               >  SRR19973065.1740720/1‑150 (MQ=60)
                                                          GTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCAT                                                                                          <  SRR19973065.2480680/150‑1 (MQ=60)
                                                           TCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATA                                                                                         <  SRR19973065.211730/150‑1 (MQ=60)
                                                             TTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACC                                                                                       <  SRR19973065.367327/150‑1 (MQ=60)
                                                             TTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACC                                                                                       <  SRR19973065.3082390/150‑1 (MQ=60)
                                                             TTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACC                                                                                       <  SRR19973065.367360/150‑1 (MQ=60)
                                                              TAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCC                                                                                      <  SRR19973065.2886958/150‑1 (MQ=60)
                                                                 AGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGT                                                                                   >  SRR19973065.484713/1‑150 (MQ=60)
                                                                  GGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTT                                                                                  <  SRR19973065.2508477/150‑1 (MQ=60)
                                                                  GGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTT                                                                                  <  SRR19973065.1481051/150‑1 (MQ=60)
                                                                  GGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTT                                                                                  <  SRR19973065.904024/150‑1 (MQ=60)
                                                                  GGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTATGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTT                                                                                  <  SRR19973065.1480925/150‑1 (MQ=60)
                                                                   GCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTG                                                                                 <  SRR19973065.1748494/150‑1 (MQ=60)
                                                                   GCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTG                                                                                 <  SRR19973065.3016085/150‑1 (MQ=60)
                                                                   GCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTG                                                                                 <  SRR19973065.1747926/150‑1 (MQ=60)
                                                                   GCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTG                                                                                 <  SRR19973065.1747932/150‑1 (MQ=60)
                                                                    CGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGA                                                                                <  SRR19973065.1681422/150‑1 (MQ=60)
                                                                    CGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCAGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGA                                                                                <  SRR19973065.2406333/150‑1 (MQ=60)
                                                                     GCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAG                                                                               <  SRR19973065.1462666/150‑1 (MQ=60)
                                                                      CTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGT                                                                              <  SRR19973065.1052861/150‑1 (MQ=60)
                                                                      CTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGT                                                                              <  SRR19973065.1053275/150‑1 (MQ=60)
                                                                           TAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTT                                                                         >  SRR19973065.1546347/1‑150 (MQ=60)
                                                                             ACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTA                                                                       <  SRR19973065.1943479/150‑1 (MQ=60)
                                                                                    GTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCA                                                                <  SRR19973065.3257783/150‑1 (MQ=60)
                                                                                         TGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAAC                                                           <  SRR19973065.1684358/150‑1 (MQ=60)
                                                                                         TGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAAC                                                           <  SRR19973065.1684388/150‑1 (MQ=60)
                                                                                         TGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAAC                                                           <  SRR19973065.2384433/150‑1 (MQ=60)
                                                                                             TCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGT                                                       <  SRR19973065.2113642/150‑1 (MQ=60)
                                                                                              CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      <  SRR19973065.48056/150‑1 (MQ=60)
                                                                                                 ACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCG                                                   <  SRR19973065.797051/150‑1 (MQ=60)
                                                                                                 ACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCG                                                   >  SRR19973065.1734560/1‑150 (MQ=60)
                                                                                                    CGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTTCTTCTTGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTTTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAA                                                <  SRR19973065.1493971/150‑1 (MQ=60)
                                                                                                    CGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAA                                                >  SRR19973065.1701832/1‑150 (MQ=60)
                                                                                                      CGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAT                                              >  SRR19973065.894770/1‑150 (MQ=60)
                                                                                                      CGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAT                                              >  SRR19973065.1661367/1‑150 (MQ=60)
                                                                                                       GTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATG                                             <  SRR19973065.1703823/150‑1 (MQ=60)
                                                                                                             GCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTT                                       <  SRR19973065.1300089/150‑1 (MQ=60)
                                                                                                                TTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAC                                    <  SRR19973065.2880221/150‑1 (MQ=60)
                                                                                                                TTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAC                                    >  SRR19973065.2520422/1‑150 (MQ=60)
                                                                                                                TTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAC                                    <  SRR19973065.3389042/150‑1 (MQ=60)
                                                                                                                TTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGGTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAC                                    <  SRR19973065.3389056/150‑1 (MQ=60)
                                                                                                                   GGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTT                                 <  SRR19973065.1951753/150‑1 (MQ=60)
                                                                                                                   GGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTT                                 <  SRR19973065.1216648/150‑1 (MQ=60)
                                                                                                                   GGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTT                                 <  SRR19973065.985745/150‑1 (MQ=60)
                                                                                                                   GGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTT                                 <  SRR19973065.985637/150‑1 (MQ=60)
                                                                                                                         CGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCC                           <  SRR19973065.1393812/150‑1 (MQ=60)
                                                                                                                                 CGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTT                   >  SRR19973065.2543823/1‑150 (MQ=60)
                                                                                                                                  GCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTG                  >  SRR19973065.1457/1‑150 (MQ=60)
                                                                                                                                   CTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGG                 <  SRR19973065.2049402/150‑1 (MQ=60)
                                                                                                                                   CTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGG                 <  SRR19973065.2049422/150‑1 (MQ=60)
                                                                                                                                   CTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGG                 <  SRR19973065.1079834/150‑1 (MQ=60)
                                                                                                                                   CTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGG                 <  SRR19973065.2049446/150‑1 (MQ=60)
                                                                                                                                    TAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGG                >  SRR19973065.312269/1‑150 (MQ=60)
                                                                                                                                       CGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGAT             <  SRR19973065.1198827/150‑1 (MQ=60)
                                                                                                                                       CGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGAT             >  SRR19973065.974965/1‑150 (MQ=60)
                                                                                                                                        GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            >  SRR19973065.3386142/1‑150 (MQ=60)
                                                                                                                                        GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            >  SRR19973065.1114492/1‑150 (MQ=60)
                                                                                                                                              GTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGAT      <  SRR19973065.2907436/150‑1 (MQ=60)
                                                                                                                                                  TTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  <  SRR19973065.1143693/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  >  CP009273/253709‑254004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑