Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 253,855 G→T 100% R281L (CGT→CTT)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273253,8550GT97.8% 880.4 / 6.5 277R281L (CGT→CTT) frsAfermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base G (4/2);  new base T (126/145);  total (130/147)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 277 total aligned reads displayed.

CGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGAT  >  CP009273/253708‑254000
                                                                                                                                                   |                                                                                                                                                 
 ggTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCt                                                                                                                                                >  2:1530897/1‑150 (MQ=255)
  gTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTg                                                                                                                                               <  1:4096849/150‑1 (MQ=255)
   tGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTgg                                                                                                                                              >  1:691285/1‑150 (MQ=255)
    gggCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGc                                                                                                                                             >  2:3121690/1‑150 (MQ=255)
     ggCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCa                                                                                                                                            <  1:1276881/150‑1 (MQ=255)
      gCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCAt                                                                                                                                           >  2:2549528/1‑150 (MQ=255)
          ttcttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCt                                                                                                                                       <  1:4676613/150‑1 (MQ=255)
           tcttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCtt                                                                                                                                      >  1:1130094/1‑150 (MQ=255)
               cAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAt                                                                                                                                  <  1:673233/150‑1 (MQ=255)
               cAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAt                                                                                                                                  <  1:2347265/150‑1 (MQ=255)
                    tGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcgccg                                                                                                                             >  1:1291431/1‑150 (MQ=255)
                    tGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcgccg                                                                                                                             >  1:4934936/1‑150 (MQ=255)
                      gAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcgccgca                                                                                                                           <  2:888841/150‑2 (MQ=255)
                      gAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCcgcg                                                                                                                           >  2:4902625/1‑150 (MQ=255)
                       aaGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGt                                                                                                                          >  1:501575/1‑150 (MQ=255)
                        aGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGtn                                                                                                                         <  1:3375104/150‑2 (MQ=255)
                         gCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCt                                                                                                                        <  2:431019/150‑1 (MQ=255)
                          cTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTg                                                                                                                       >  2:683967/1‑150 (MQ=255)
                           tCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGa                                                                                                                      <  2:2420045/150‑1 (MQ=255)
                               ccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGc                                                                                                                  >  2:1752745/1‑150 (MQ=255)
                               ccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGc                                                                                                                  >  1:4797424/1‑150 (MQ=255)
                                cAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCg                                                                                                                 <  2:4956577/150‑1 (MQ=255)
                                 aGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCgg                                                                                                                >  2:4804286/1‑150 (MQ=255)
                                  ggACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGt                                                                                                               >  2:4748168/1‑150 (MQ=255)
                                  ggACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGt                                                                                                               <  1:351737/150‑1 (MQ=255)
                                         aGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCNTACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGt                                                                                                        <  1:2678894/150‑1 (MQ=255)
                                           ccTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCt                                                                                                      >  2:3734008/1‑150 (MQ=255)
                                            cTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCtt                                                                                                     >  2:1776267/1‑150 (MQ=255)
                                             tGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                    >  1:3680008/1‑150 (MQ=255)
                                             tGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                    <  1:4717890/150‑1 (MQ=255)
                                              gTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTgg                                                                                                   >  2:1867610/1‑150 (MQ=255)
                                                 gCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTcc                                                                                                >  2:1637603/1‑150 (MQ=255)
                                                   aTCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCgg                                                                                              >  1:573528/1‑150 (MQ=255)
                                                   aTCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCgg                                                                                              >  1:2278707/1‑150 (MQ=255)
                                                    tCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGt                                                                                             <  2:3786106/150‑1 (MQ=255)
                                                     taGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTa                                                                                            >  2:1010474/2‑150 (MQ=255)
                                                       gCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGt                                                                                          >  1:3804850/1‑150 (MQ=255)
                                                         aCGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTc                                                                                        <  2:2635297/150‑1 (MQ=255)
                                                            tCTTAAAGGCGCTGCCTAACGTACCGGGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATa                                                                                     <  2:4299021/150‑1 (MQ=255)
                                                               tAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAccc                                                                                  >  1:1330229/1‑150 (MQ=255)
                                                               tAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAccc                                                                                  >  1:1730453/1‑150 (MQ=255)
                                                                aaaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCt                                                                                 <  1:2176478/150‑1 (MQ=255)
                                                                aaaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCt                                                                                 <  2:5106021/150‑1 (MQ=255)
                                                                  aGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGt                                                                               >  2:2373144/1‑150 (MQ=255)
                                                                  aGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGt                                                                               >  1:5059433/1‑150 (MQ=255)
                                                                    gcgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTg                                                                             <  2:611802/150‑1 (MQ=255)
                                                                    gcgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTg                                                                             <  2:1652488/150‑1 (MQ=255)
                                                                     cgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGa                                                                            >  2:4775206/1‑150 (MQ=255)
                                                                      gcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAg                                                                           >  1:3859770/1‑150 (MQ=255)
                                                                        tGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTg                                                                         >  1:2635430/1‑150 (MQ=255)
                                                                        tGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTg                                                                         <  1:1628352/150‑1 (MQ=255)
                                                                        tGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTg                                                                         >  2:2146142/1‑150 (MQ=255)
                                                                          ccTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAt                                                                       >  2:2681753/1‑150 (MQ=255)
                                                                          ccTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAt                                                                       >  1:3160061/1‑150 (MQ=255)
                                                                           cTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtt                                                                      <  2:2975491/150‑1 (MQ=255)
                                                                            tAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAttt                                                                     <  2:3884006/150‑1 (MQ=255)
                                                                             aaCGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtttt                                                                    >  2:2355255/1‑150 (MQ=255)
                                                                                gTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAg                                                                 >  1:586285/1‑150 (MQ=255)
                                                                                 tACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGt                                                                <  1:3006241/150‑1 (MQ=255)
                                                                                    cGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGcc                                                             <  2:397406/150‑1 (MQ=255)
                                                                                       gggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGc                                                          >  1:4525202/1‑150 (MQ=255)
                                                                                         gtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTGTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCaa                                                        >  1:3091788/1‑150 (MQ=255)
                                                                                            gATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAg                                                     <  2:2784570/150‑1 (MQ=255)
                                                                                                  acTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCg                                               >  1:1066123/1‑150 (MQ=255)
                                                                                                  acTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCg                                               <  2:3332252/150‑1 (MQ=255)
                                                                                                   cTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCgg                                              >  1:460377/1‑150 (MQ=255)
                                                                                                       cgtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAt                                          >  2:764007/1‑150 (MQ=255)
                                                                                                       cgtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAt                                          <  2:3462819/150‑1 (MQ=255)
                                                                                                        gtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATg                                         >  1:846160/1‑150 (MQ=255)
                                                                                                          cgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTa                                       >  1:4311420/1‑150 (MQ=255)
                                                                                                            cgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATc                                     <  2:5162405/150‑1 (MQ=255)
                                                                                                             ggCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCt                                    >  1:3964970/1‑150 (MQ=255)
                                                                                                              gCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCtt                                   >  1:2928735/1‑150 (MQ=255)
                                                                                                               ccTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                  <  2:4256971/150‑1 (MQ=255)
                                                                                                               ccTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                  <  2:4605538/150‑1 (MQ=255)
                                                                                                                cTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGa                                 >  1:1950282/1‑150 (MQ=255)
                                                                                                                    ggTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGtt                             >  2:5047365/1‑150 (MQ=255)
                                                                                                                      tttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCt                           <  1:1260616/150‑1 (MQ=255)
                                                                                                                      tttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCt                           >  2:1904389/1‑150 (MQ=255)
                                                                                                                       ttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTg                          >  1:3585926/1‑150 (MQ=255)
                                                                                                                        tCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTgg                         <  1:1851800/150‑1 (MQ=255)
                                                                                                                           gTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCa                      <  2:356275/150‑1 (MQ=255)
                                                                                                                           gTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCa                      <  2:4175809/150‑1 (MQ=255)
                                                                                                                            tttCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAg                     <  2:1526074/150‑1 (MQ=255)
                                                                                                                              tCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTc                   >  1:3443266/1‑150 (MQ=255)
                                                                                                                              tCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTc                   <  2:3554809/150‑1 (MQ=255)
                                                                                                                               cGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCg                  >  2:3222701/1‑150 (MQ=255)
                                                                                                                               cGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCg                  <  2:123596/150‑1 (MQ=255)
                                                                                                                               cGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCg                  <  1:2096411/150‑1 (MQ=255)
                                                                                                                                ggCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGt                 >  2:371864/1‑150 (MQ=255)
                                                                                                                                 gcgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGtt                <  2:1045627/150‑1 (MQ=255)
                                                                                                                                         gTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATg        >  2:701458/1‑150 (MQ=255)
                                                                                                                                          ttGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGc       >  2:4583835/1‑150 (MQ=255)
                                                                                                                                          ttGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGc       <  1:1546832/150‑1 (MQ=255)
                                                                                                                                           tGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCa      >  2:2657061/1‑150 (MQ=255)
                                                                                                                                            gCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCat     <  1:2456079/150‑1 (MQ=255)
                                                                                                                                             ccGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCatg    <  1:1652207/150‑1 (MQ=255)
                                                                                                                                              cGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCatga   <  2:2009542/150‑1 (MQ=255)
                                                                                                                                               gTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCatgat  <  2:5059433/150‑1 (MQ=255)
                                                                                                                                               gTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTGCTGGCCAGTCGTTTGGGGATGCatgat  >  1:923000/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGAT  >  CP009273/253708‑254000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCT  >  CP009273/253706‑254003
                                                                                                                                                     |                                                                                                                                                    
GTCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCT                                                                                                                                                      >  SRR19973063.2494718/1‑150 (MQ=60)
GTCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCT                                                                                                                                                      <  SRR19973063.2669487/150‑1 (MQ=60)
GTCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCT                                                                                                                                                      <  SRR19973063.5141700/150‑1 (MQ=60)
 TCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTT                                                                                                                                                     <  SRR19973063.4742913/150‑1 (MQ=60)
  CGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTC                                                                                                                                                    <  SRR19973063.4716489/150‑1 (MQ=60)
   GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCT                                                                                                                                                   <  SRR19973063.2116069/150‑1 (MQ=60)
   GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCT                                                                                                                                                   <  SRR19973063.2525550/150‑1 (MQ=60)
    GTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTG                                                                                                                                                  <  SRR19973063.4109976/150‑1 (MQ=60)
     TGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGG                                                                                                                                                 >  SRR19973063.693318/1‑150 (MQ=60)
     TGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTGTGGTTTACGTTTCGGCGCTAACGTTGCCGTGCTTCTGG                                                                                                                                                 >  SRR19973063.693163/1‑150 (MQ=60)
       GGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCA                                                                                                                                               <  SRR19973063.1280685/150‑1 (MQ=60)
         CTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATA                                                                                                                                             >  SRR19973063.4591391/1‑150 (MQ=60)
            TTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCT                                                                                                                                          <  SRR19973063.4691529/150‑1 (MQ=60)
             TCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTT                                                                                                                                         <  SRR19973063.5156942/150‑1 (MQ=60)
             TCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTT                                                                                                                                         >  SRR19973063.1133521/1‑150 (MQ=60)
                 CAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAT                                                                                                                                     <  SRR19973063.675202/150‑1 (MQ=60)
                 CAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAT                                                                                                                                     <  SRR19973063.2354775/150‑1 (MQ=60)
                 CAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAT                                                                                                                                     <  SRR19973063.2354657/150‑1 (MQ=60)
                  AAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATC                                                                                                                                    >  SRR19973063.410113/1‑150 (MQ=60)
                   TAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCG                                                                                                                                   >  SRR19973063.3386596/1‑150 (MQ=60)
                     ATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCC                                                                                                                                 >  SRR19973063.2389746/1‑150 (MQ=60)
                      TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCG                                                                                                                                <  SRR19973063.635927/150‑1 (MQ=60)
                      TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCG                                                                                                                                <  SRR19973063.458232/150‑1 (MQ=60)
                      TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCG                                                                                                                                >  SRR19973063.1645191/1‑150 (MQ=60)
                      TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCG                                                                                                                                >  SRR19973063.3938041/1‑150 (MQ=60)
                      TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCG                                                                                                                                >  SRR19973063.4950877/1‑150 (MQ=60)
                      TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCG                                                                                                                                >  SRR19973063.1295274/1‑150 (MQ=60)
                      TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCG                                                                                                                                >  SRR19973063.124002/1‑150 (MQ=60)
                         AAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGT                                                                                                                             >  SRR19973063.503021/1‑150 (MQ=60)
                          TGCTCACCCAGGACTCCAGCCTGGTGCATCAGCACGTCTTAAAGGCGCGGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTC                                                                                                                            <  SRR19973063.2796310/150‑1 (MQ=60)
                          AGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTN                                                                                                                            <  SRR19973063.3385855/150‑1 (MQ=60)
                            CTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTG                                                                                                                          <  SRR19973063.2927520/150‑1 (MQ=60)
                                 CCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGC                                                                                                                     <  SRR19973063.3110090/150‑1 (MQ=60)
                                 CCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGC                                                                                                                     >  SRR19973063.4812759/1‑150 (MQ=60)
                                 CCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGC                                                                                                                     <  SRR19973063.1087736/150‑1 (MQ=60)
                                 CCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGC                                                                                                                     >  SRR19973063.1293625/1‑150 (MQ=60)
                                 CCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGC                                                                                                                     >  SRR19973063.131402/1‑150 (MQ=60)
                                  CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                    <  SRR19973063.1235263/150‑1 (MQ=60)
                                    GGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGT                                                                                                                  <  SRR19973063.352665/150‑1 (MQ=60)
                                    GGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGT                                                                                                                  <  SRR19973063.352750/150‑1 (MQ=60)
                                      ACGCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTG                                                                                                                <  SRR19973063.1422243/150‑1 (MQ=60)
                                        TCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCC                                                                                                              >  SRR19973063.2350913/1‑150 (MQ=60)
                                        TCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCC                                                                                                              >  SRR19973063.2655009/1‑150 (MQ=60)
                                          CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                            <  SRR19973063.439242/150‑1 (MQ=60)
                                           AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCNTACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGT                                                                                                           <  SRR19973063.2687514/150‑1 (MQ=60)
                                           AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGT                                                                                                           <  SRR19973063.3441399/150‑1 (MQ=60)
                                            GCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTC                                                                                                          <  SRR19973063.3473871/150‑1 (MQ=60)
                                              CTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTT                                                                                                        <  SRR19973063.1619571/150‑1 (MQ=60)
                                               TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                       <  SRR19973063.4732935/150‑1 (MQ=60)
                                               TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                       >  SRR19973063.3691790/1‑150 (MQ=60)
                                               TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                       <  SRR19973063.4733005/150‑1 (MQ=60)
                                                GTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGG                                                                                                      <  SRR19973063.2052783/150‑1 (MQ=60)
                                                    CATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCG                                                                                                  <  SRR19973063.759058/150‑1 (MQ=60)
                                                    CATCAGCACGTCTTAAAGGCGCTGACTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCG                                                                                                  >  SRR19973063.3864576/1‑150 (MQ=60)
                                                     ATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGG                                                                                                 >  SRR19973063.2285981/1‑150 (MQ=60)
                                                     ATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGG                                                                                                 >  SRR19973063.575219/1‑150 (MQ=60)
                                                         GCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGT                                                                                             >  SRR19973063.3816966/1‑150 (MQ=60)
                                                          CACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTT                                                                                            <  SRR19973063.287565/150‑1 (MQ=60)
                                                          CACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTGTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTT                                                                                            >  SRR19973063.257220/1‑150 (MQ=60)
                                                               CTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAG                                                                                       <  SRR19973063.4284549/150‑1 (MQ=60)
                                                               CTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAG                                                                                       <  SRR19973063.4284451/150‑1 (MQ=60)
                                                                 TAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCC                                                                                     >  SRR19973063.1334212/1‑150 (MQ=60)
                                                                 TAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCC                                                                                     >  SRR19973063.1735729/1‑150 (MQ=60)
                                                                  AAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCT                                                                                    <  SRR19973063.2183288/150‑1 (MQ=60)
                                                                    AGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGT                                                                                  >  SRR19973063.5075721/1‑150 (MQ=60)
                                                                        GCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAG                                                                              >  SRR19973063.3872046/1‑150 (MQ=60)
                                                                        GCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAG                                                                              >  SRR19973063.1633406/1‑150 (MQ=60)
                                                                        GCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAG                                                                              >  SRR19973063.4853964/1‑150 (MQ=60)
                                                                        GCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAG                                                                              >  SRR19973063.3872066/1‑150 (MQ=60)
                                                                        GCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAG                                                                              <  SRR19973063.2032939/150‑1 (MQ=60)
                                                                          TGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTG                                                                            <  SRR19973063.4918453/150‑1 (MQ=60)
                                                                          TGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTG                                                                            >  SRR19973063.2643842/1‑150 (MQ=60)
                                                                          TGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTG                                                                            <  SRR19973063.1633325/150‑1 (MQ=60)
                                                                           GCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGA                                                                           <  SRR19973063.587473/150‑1 (MQ=60)
                                                                           GCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGA                                                                           <  SRR19973063.587447/150‑1 (MQ=60)
                                                                            CCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAT                                                                          >  SRR19973063.3170232/1‑150 (MQ=60)
                                                                              TAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTT                                                                        <  SRR19973063.5003211/150‑1 (MQ=60)
                                                                               AACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTT                                                                       <  SRR19973063.3680189/150‑1 (MQ=60)
                                                                                  GTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAG                                                                    >  SRR19973063.588016/1‑150 (MQ=60)
                                                                                   TACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGT                                                                   <  SRR19973063.3015835/150‑1 (MQ=60)
                                                                                   TACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGT                                                                   <  SRR19973063.1677256/150‑1 (MQ=60)
                                                                                       GTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCA                                                               <  SRR19973063.1892701/150‑1 (MQ=60)
                                                                                        TGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAG                                                              <  SRR19973063.547032/150‑1 (MQ=60)
                                                                                         GGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGC                                                             >  SRR19973063.4539715/1‑150 (MQ=60)
                                                                                           GTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAA                                                           <  SRR19973063.3705540/150‑1 (MQ=60)
                                                                                           GTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTGTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAA                                                           >  SRR19973063.3101734/1‑150 (MQ=60)
                                                                                            TGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAAC                                                          >  SRR19973063.961474/1‑150 (MQ=60)
                                                                                              GATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAG                                                        <  SRR19973063.3043771/150‑1 (MQ=60)
                                                                                              GATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAG                                                        <  SRR19973063.298897/150‑1 (MQ=60)
                                                                                                 CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                     <  SRR19973063.5016866/150‑1 (MQ=60)
                                                                                                 CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                     <  SRR19973063.1419597/150‑1 (MQ=60)
                                                                                                 CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                     >  SRR19973063.4712249/1‑150 (MQ=60)
                                                                                                   CACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCC                                                   <  SRR19973063.1294741/150‑1 (MQ=60)
                                                                                                   CACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCC                                                   <  SRR19973063.658196/150‑1 (MQ=60)
                                                                                                   CACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCC                                                   <  SRR19973063.3334793/150‑1 (MQ=60)
                                                                                                    ACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCG                                                  >  SRR19973063.1069360/1‑150 (MQ=60)
                                                                                                     CTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGG                                                 >  SRR19973063.461674/1‑150 (MQ=60)
                                                                                                        GCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAA                                              <  SRR19973063.1770641/150‑1 (MQ=60)
                                                                                                        GCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAA                                              <  SRR19973063.1770603/150‑1 (MQ=60)
                                                                                                          GTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATG                                            >  SRR19973063.851170/1‑150 (MQ=60)
                                                                                                          GTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATG                                            >  SRR19973063.848602/1‑150 (MQ=60)
                                                                                                            CGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTA                                          >  SRR19973063.4325120/1‑150 (MQ=60)
                                                                                                               GGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCT                                       >  SRR19973063.3977657/1‑150 (MQ=60)
                                                                                                                GCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTT                                      >  SRR19973063.2938070/1‑150 (MQ=60)
                                                                                                                GCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTT                                      >  SRR19973063.2938076/1‑150 (MQ=60)
                                                                                                                 CCTTGGGTTTCCGTTTCGGCGGTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATATTG                                     <  SRR19973063.4763291/150‑1 (MQ=60)
                                                                                                                  CTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGA                                    >  SRR19973063.1556621/1‑150 (MQ=60)
                                                                                                                  CTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGA                                    >  SRR19973063.2180168/1‑150 (MQ=60)
                                                                                                                  CTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGA                                    >  SRR19973063.1956388/1‑150 (MQ=60)
                                                                                                                    TTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACG                                  >  SRR19973063.914860/1‑150 (MQ=60)
                                                                                                                       GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTC                               <  SRR19973063.4656968/150‑1 (MQ=60)
                                                                                                                       GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTC                               >  SRR19973063.3844181/1‑150 (MQ=60)
                                                                                                                       GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTC                               <  SRR19973063.1895973/150‑1 (MQ=60)
                                                                                                                        TTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCT                              <  SRR19973063.697351/150‑1 (MQ=60)
                                                                                                                        TTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCT                              <  SRR19973063.1264367/150‑1 (MQ=60)
                                                                                                                        TTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCT                              <  SRR19973063.1264371/150‑1 (MQ=60)
                                                                                                                         TTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTG                             <  SRR19973063.3335331/150‑1 (MQ=60)
                                                                                                                         TTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTG                             >  SRR19973063.3597421/1‑150 (MQ=60)
                                                                                                                          TCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGG                            <  SRR19973063.1857583/150‑1 (MQ=60)
                                                                                                                                TCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTC                      >  SRR19973063.3454214/1‑150 (MQ=60)
                                                                                                                                 CGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCG                     <  SRR19973063.2102960/150‑1 (MQ=60)
                                                                                                                                  GGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGT                    <  SRR19973063.3778172/150‑1 (MQ=60)
                                                                                                                                      CTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGG                >  SRR19973063.1267677/1‑150 (MQ=60)
                                                                                                                                       TAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGG               <  SRR19973063.3582668/150‑1 (MQ=60)
                                                                                                                                       TAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGG               <  SRR19973063.3582544/150‑1 (MQ=60)
                                                                                                                                        AACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGG              >  SRR19973063.3016384/1‑150 (MQ=60)
                                                                                                                                          CGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGAT            <  SRR19973063.5034123/150‑1 (MQ=60)
                                                                                                                                            TTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGC          <  SRR19973063.1551572/150‑1 (MQ=60)
                                                                                                                                              GCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCAT        <  SRR19973063.2463923/150‑1 (MQ=60)
                                                                                                                                               CCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATG       >  SRR19973063.2358464/1‑150 (MQ=60)
                                                                                                                                               CCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATG       <  SRR19973063.1657248/150‑1 (MQ=60)
                                                                                                                                                CGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGA      >  SRR19973063.2224259/1‑150 (MQ=60)
                                                                                                                                                CGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGA      >  SRR19973063.4428697/1‑150 (MQ=60)
                                                                                                                                                 GTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTGCTGGCCAGTCGTTTGGGGATGCATGAT     >  SRR19973063.925735/1‑150 (MQ=60)
                                                                                                                                                   GCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGC   >  SRR19973063.3236070/1‑150 (MQ=60)
                                                                                                                                                    CTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCT  >  SRR19973063.4919072/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
GTCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCT  >  CP009273/253706‑254003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑