Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 253,855 G→T 100% R281L (CGT→CTT)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273253,8550GT96.8% 669.4 / 12.1 219R281L (CGT→CTT) frsAfermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base G (3/4);  new base T (103/108);  total (107/112)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 219 total aligned reads displayed.

GTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGA  >  CP009273/253710‑253999
                                                                                                                                                 |                                                                                                                                                
 tGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCt                                                                                                                                               >  2:2730157/1‑148 (MQ=255)
   ggCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTgg                                                                                                                                             >  2:1737401/1‑148 (MQ=255)
   ggCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTgg                                                                                                                                             >  1:3468040/1‑148 (MQ=255)
    gCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGc                                                                                                                                            <  2:1336349/148‑1 (MQ=255)
    gCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGc                                                                                                                                            >  2:3427323/1‑148 (MQ=255)
    gCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGc                                                                                                                                            <  1:2034989/148‑1 (MQ=255)
        ttcttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATAc                                                                                                                                        <  2:1068826/148‑1 (MQ=255)
         tcttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATAcc                                                                                                                                       <  2:856661/148‑1 (MQ=255)
             cAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGa                                                                                                                                   <  1:3048417/148‑1 (MQ=255)
             cAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGa                                                                                                                                   >  1:816409/1‑148 (MQ=255)
             cAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGa                                                                                                                                   <  2:3390043/148‑1 (MQ=255)
             cAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGa                                                                                                                                   <  1:1142690/148‑1 (MQ=255)
               aaaTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAt                                                                                                                                 <  2:2236742/148‑1 (MQ=255)
                aaTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATc                                                                                                                                >  2:260057/1‑148 (MQ=255)
                aaTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATc                                                                                                                                >  1:2316004/1‑148 (MQ=255)
                 aTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcg                                                                                                                               >  1:42821/1‑148 (MQ=255)
                  tGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcgc                                                                                                                              >  1:210085/1‑148 (MQ=255)
                   ggAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcgcc                                                                                                                             >  1:903458/1‑148 (MQ=255)
                       gCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGt                                                                                                                         <  1:690140/148‑1 (MQ=255)
                         tCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCt                                                                                                                       <  2:601116/148‑1 (MQ=255)
                            cccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGaa                                                                                                                    <  1:3219944/148‑1 (MQ=255)
                            cccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGaa                                                                                                                    <  1:2779042/148‑1 (MQ=255)
                            cccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGaa                                                                                                                    <  1:2840909/148‑1 (MQ=255)
                            cccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGaa                                                                                                                    <  1:1316599/148‑1 (MQ=255)
                            cccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGaa                                                                                                                    <  1:3491477/148‑1 (MQ=255)
                               aGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGc                                                                                                                 >  1:1020380/1‑148 (MQ=255)
                               aGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGc                                                                                                                 >  2:3212834/1‑148 (MQ=255)
                                ggACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCg                                                                                                                >  1:2069918/1‑148 (MQ=255)
                                 gACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGGTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCgg                                                                                                               <  2:2561631/148‑1 (MQ=255)
                                 gACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCGTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCgg                                                                                                               >  2:1776050/1‑148 (MQ=255)
                                    tCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTg                                                                                                            >  2:3163496/1‑148 (MQ=255)
                                     ccAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGc                                                                                                           <  2:3540953/148‑1 (MQ=255)
                                        gCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTg                                                                                                        >  1:1694444/1‑148 (MQ=255)
                                         ccTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGt                                                                                                       >  2:664612/1‑148 (MQ=255)
                                         ccTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTCGCATACCTTGAATCGCCGCGTCTGAAAGAGGTTGCCAGt                                                                                                       >  1:2285843/1‑148 (MQ=255)
                                          cTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTc                                                                                                      <  1:1852867/148‑1 (MQ=255)
                                           tGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCt                                                                                                     <  2:955129/148‑1 (MQ=255)
                                           tGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCt                                                                                                     >  1:983225/1‑148 (MQ=255)
                                           tGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCt                                                                                                     >  1:2849773/1‑148 (MQ=255)
                                             ttGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                   <  1:800064/148‑1 (MQ=255)
                                                cATCAGCACGTCTTACAGGCGCTGCCTAACGTACCGTGTGTGTATCACACTCGCGTCGCGGCCTTTGGTTTCGGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTATTGGTc                                                                                                <  2:1215596/148‑1 (MQ=255)
                                                  tCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCg                                                                                              >  1:335437/1‑148 (MQ=255)
                                                      cACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGACGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAg                                                                                          >  2:2002344/1‑148 (MQ=255)
                                                         gTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTc                                                                                       <  2:1936339/148‑1 (MQ=255)
                                                          tCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCa                                                                                      <  1:3028809/148‑1 (MQ=255)
                                                          tCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCa                                                                                      <  2:1108827/148‑1 (MQ=255)
                                                            ttAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATa                                                                                    <  2:1739345/148‑1 (MQ=255)
                                                              aaaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAcc                                                                                  <  1:3777048/148‑1 (MQ=255)
                                                              aaaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATAcc                                                                                  <  1:3705785/148‑1 (MQ=255)
                                                                aGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCAGAAAGCGGTAGCCTGTCTTGGTCCGGTAGTTCATAACCt                                                                                >  2:1415053/1‑148 (MQ=255)
                                                                 ggCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                               >  1:2915998/1‑148 (MQ=255)
                                                                  gcgcTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGt                                                                              >  2:962378/1‑148 (MQ=255)
                                                                         cTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGa                                                                       <  2:3001765/148‑1 (MQ=255)
                                                                         cTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGa                                                                       >  2:2493931/1‑148 (MQ=255)
                                                                         cTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGa                                                                       >  1:2310157/1‑148 (MQ=255)
                                                                         cTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGa                                                                       >  2:653717/1‑148 (MQ=255)
                                                                           aaCGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtt                                                                     <  2:1523187/148‑1 (MQ=255)
                                                                             cGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtttt                                                                   >  1:1224842/1‑148 (MQ=255)
                                                                              gTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCTGCGATAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTa                                                                  <  1:1964243/148‑1 (MQ=255)
                                                                               tACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTaa                                                                 <  2:903458/148‑1 (MQ=255)
                                                                                aCCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAg                                                                <  1:3185880/148‑1 (MQ=255)
                                                                                 ccGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGt                                                               <  1:572873/148‑1 (MQ=255)
                                                                                      ggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAg                                                          >  2:597037/1‑148 (MQ=255)
                                                                                           aTCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACa                                                     >  2:2914185/1‑148 (MQ=255)
                                                                                           aTCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACa                                                     >  2:1456492/1‑148 (MQ=255)
                                                                                            tCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAg                                                    <  2:3053241/148‑1 (MQ=255)
                                                                                             cacaCTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAgg                                                   >  2:2070777/1‑148 (MQ=255)
                                                                                             cacaCTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAgg                                                   <  1:974152/148‑1 (MQ=255)
                                                                                              acacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGt                                                  >  1:1796500/1‑148 (MQ=255)
                                                                                               cacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTg                                                 <  1:1782055/148‑1 (MQ=255)
                                                                                                      gtcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGTTTTTAAGTGCCAGCAACAGGTGCCGGaaa                                          <  2:161008/148‑1 (MQ=255)
                                                                                                       tcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGaact                                         >  2:293896/1‑146 (MQ=255)
                                                                                                       tcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAt                                         <  2:3326000/148‑1 (MQ=255)
                                                                                                          cgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTa                                      <  1:3641532/148‑1 (MQ=255)
                                                                                                          cgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTa                                      <  1:920419/148‑1 (MQ=255)
                                                                                                            gCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATc                                    <  2:2792994/148‑1 (MQ=255)
                                                                                                              cTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCtt                                  >  2:3199876/1‑148 (MQ=255)
                                                                                                                ttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGa                                >  2:3736012/1‑148 (MQ=255)
                                                                                                                ttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGa                                >  1:711610/1‑148 (MQ=255)
                                                                                                                     ttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTc                           >  1:2164298/1‑148 (MQ=255)
                                                                                                                      tCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                 >  2:951621/1‑141 (MQ=255)
                                                                                                                      tCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                 <  1:951621/141‑1 (MQ=255)
                                                                                                                          tttCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGcc                      >  2:2492614/1‑148 (MQ=255)
                                                                                                                             cGGCGCTTACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGTTAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACACTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGt                   <  1:1415053/148‑1 (MQ=255)
                                                                                                                               gcgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCg                 <  2:1060640/148‑1 (MQ=255)
                                                                                                                               gcgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCg                 <  2:1964895/148‑1 (MQ=255)
                                                                                                                               gcgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCg                 <  2:1060526/148‑1 (MQ=255)
                                                                                                                                cgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGt                <  2:3241520/148‑1 (MQ=255)
                                                                                                                                cgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGt                <  2:2315999/148‑1 (MQ=255)
                                                                                                                                cgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGt                <  1:2276607/148‑1 (MQ=255)
                                                                                                                                  cTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGttt              <  2:295973/148‑1 (MQ=255)
                                                                                                                                  cTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGttt              <  1:3212853/148‑1 (MQ=255)
                                                                                                                                       gTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGACAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGa         <  2:2285843/148‑1 (MQ=255)
                                                                                                                                       gTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGa         <  1:3444246/148‑1 (MQ=255)
                                                                                                                                         tGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATg       >  1:1863264/1‑148 (MQ=255)
                                                                                                                                         tGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTGTGGGGATg       >  1:3335600/1‑148 (MQ=255)
                                                                                                                                         tGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACACTGTTGAGTGATTTTAAGTGACAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCAAGTCGTTTGTGGATg       >  1:2370802/1‑148 (MQ=255)
                                                                                                                                           ccGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCa     <  1:1118712/148‑1 (MQ=255)
                                                                                                                                            cGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCat    >  2:3813749/1‑148 (MQ=255)
                                                                                                                                              tGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCatga  <  2:409404/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGA  >  CP009273/253710‑253999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  >  CP009273/253710‑254004
                                                                                                                                                 |                                                                                                                                                     
GTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTG                                                                                                                                                   >  SRR19973069.1208543/1‑150 (MQ=60)
 TGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGG                                                                                                                                                  >  SRR19973069.3490475/1‑150 (MQ=60)
    GCTTTTCTTCAAACTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCAT                                                                                                                                               <  SRR19973069.955880/150‑1 (MQ=60)
    GCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCAT                                                                                                                                               <  SRR19973069.2049129/150‑1 (MQ=60)
    GCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCAT                                                                                                                                               <  SRR19973069.2049119/150‑1 (MQ=60)
        TTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGTATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCT                                                                                                                                           <  SRR19973069.3486905/150‑1 (MQ=60)
        TTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCT                                                                                                                                           >  SRR19973069.297995/1‑150 (MQ=60)
          CTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCGTCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTG                                                                                                                                         >  SRR19973069.3237560/1‑150 (MQ=60)
           TTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGA                                                                                                                                        >  SRR19973069.822473/1‑150 (MQ=60)
           TTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGA                                                                                                                                        >  SRR19973069.822177/1‑150 (MQ=60)
             CAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGANT                                                                                                                                      <  SRR19973069.2916302/150‑1 (MQ=60)
             CAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAT                                                                                                                                      <  SRR19973069.1150666/150‑1 (MQ=60)
             CAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAT                                                                                                                                      <  SRR19973069.3068606/150‑1 (MQ=60)
             CAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAAT                                                                                                                                      <  SRR19973069.3068602/150‑1 (MQ=60)
              AAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATC                                                                                                                                     >  SRR19973069.3262737/1‑150 (MQ=60)
              AAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATC                                                                                                                                     >  SRR19973069.2331763/1‑150 (MQ=60)
              AAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATC                                                                                                                                     >  SRR19973069.2331768/1‑150 (MQ=60)
               AAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCG                                                                                                                                    <  SRR19973069.1290509/150‑1 (MQ=60)
               AAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCG                                                                                                                                    >  SRR19973069.43374/1‑150 (MQ=60)
                AATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGC                                                                                                                                   >  SRR19973069.211747/1‑150 (MQ=60)
                 ATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCC                                                                                                                                  >  SRR19973069.909658/1‑150 (MQ=60)
                       GCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCT                                                                                                                            <  SRR19973069.694971/150‑1 (MQ=60)
                            CCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAG                                                                                                                       <  SRR19973069.2797549/150‑1 (MQ=60)
                            CCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAG                                                                                                                       <  SRR19973069.2859652/150‑1 (MQ=60)
                            CCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAG                                                                                                                       <  SRR19973069.3240974/150‑1 (MQ=60)
                            CCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAG                                                                                                                       <  SRR19973069.2859782/150‑1 (MQ=60)
                            CCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAG                                                                                                                       <  SRR19973069.1325629/150‑1 (MQ=60)
                            CCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAG                                                                                                                       <  SRR19973069.3513992/150‑1 (MQ=60)
                             CCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGC                                                                                                                      >  SRR19973069.1027437/1‑150 (MQ=60)
                              CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                     >  SRR19973069.2084266/1‑150 (MQ=60)
                              CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                     >  SRR19973069.1533681/1‑150 (MQ=60)
                               AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGCCTGAAAGCGG                                                                                                                    >  SRR19973069.2503689/1‑150 (MQ=60)
                                      CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             >  SRR19973069.3500893/1‑150 (MQ=60)
                                      CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             <  SRR19973069.3464362/150‑1 (MQ=60)
                                      CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             >  SRR19973069.1706284/1‑150 (MQ=60)
                                      CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             >  SRR19973069.2304975/1‑150 (MQ=60)
                                       AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGT                                                                                                            >  SRR19973069.2301345/1‑150 (MQ=60)
                                       AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTCGCATACCTTGAATCGCCGCGTCTGAAAGAGGTTGCCAGT                                                                                                            >  SRR19973069.2301365/1‑150 (MQ=60)
                                         CCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCT                                                                                                          >  SRR19973069.3073446/1‑150 (MQ=60)
                                         CCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCT                                                                                                          >  SRR19973069.2868542/1‑150 (MQ=60)
                                         CCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCT                                                                                                          <  SRR19973069.3790635/150‑1 (MQ=60)
                                         CCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCT                                                                                                          >  SRR19973069.989878/1‑150 (MQ=60)
                                         CCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCT                                                                                                          >  SRR19973069.989869/1‑150 (MQ=60)
                                          CTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTT                                                                                                         <  SRR19973069.1865802/150‑1 (MQ=60)
                                          CTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTT                                                                                                         <  SRR19973069.1865949/150‑1 (MQ=60)
                                           TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                        <  SRR19973069.1977544/150‑1 (MQ=60)
                                            GTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGG                                                                                                       <  SRR19973069.2846028/150‑1 (MQ=60)
                                             TTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGT                                                                                                      <  SRR19973069.805734/150‑1 (MQ=60)
                                              TGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTC                                                                                                     >  SRR19973069.1818137/1‑150 (MQ=60)
                                                CATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCG                                                                                                   >  SRR19973069.337770/1‑150 (MQ=60)
                                                    AGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAG                                                                                               >  SRR19973069.1889873/1‑150 (MQ=60)
                                                          TCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATA                                                                                         <  SRR19973069.2002761/150‑1 (MQ=60)
                                                          TCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATA                                                                                         <  SRR19973069.3048898/150‑1 (MQ=60)
                                                              AAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCT                                                                                     <  SRR19973069.3729995/150‑1 (MQ=60)
                                                              AAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCT                                                                                     <  SRR19973069.3801578/150‑1 (MQ=60)
                                                               AAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTG                                                                                    >  SRR19973069.2935294/1‑150 (MQ=60)
                                                               AAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTG                                                                                    >  SRR19973069.2816568/1‑150 (MQ=60)
                                                                      TGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTG                                                                             <  SRR19973069.80931/150‑1 (MQ=60)
                                                                      TGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTG                                                                             <  SRR19973069.80932/150‑1 (MQ=60)
                                                                       GCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGA                                                                            >  SRR19973069.2325874/1‑150 (MQ=60)
                                                                           AACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTT                                                                        >  SRR19973069.447922/1‑150 (MQ=60)
                                                                           AACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTT                                                                        >  SRR19973069.1233212/1‑150 (MQ=60)
                                                                              GTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCTGCGATAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAG                                                                     <  SRR19973069.1977867/150‑1 (MQ=60)
                                                                              GTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAG                                                                     <  SRR19973069.702364/150‑1 (MQ=60)
                                                                                ACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTG                                                                   <  SRR19973069.3206582/150‑1 (MQ=60)
                                                                                 CCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGC                                                                  <  SRR19973069.576833/150‑1 (MQ=60)
                                                                                         GGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACA                                                          <  SRR19973069.3147862/150‑1 (MQ=60)
                                                                                            TCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGT                                                       >  SRR19973069.1809152/1‑150 (MQ=60)
                                                                                             CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCTTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      >  SRR19973069.1123402/1‑150 (MQ=60)
                                                                                             CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      <  SRR19973069.2338269/150‑1 (MQ=60)
                                                                                             CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      <  SRR19973069.2238197/150‑1 (MQ=60)
                                                                                             CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      >  SRR19973069.989229/1‑150 (MQ=60)
                                                                                             CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      <  SRR19973069.980688/150‑1 (MQ=60)
                                                                                             CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      <  SRR19973069.980727/150‑1 (MQ=60)
                                                                                               CACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCC                                                    <  SRR19973069.1794568/150‑1 (MQ=60)
                                                                                               CACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCC                                                    <  SRR19973069.1794605/150‑1 (MQ=60)
                                                                                                ACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCG                                                   <  SRR19973069.3361280/150‑1 (MQ=60)
                                                                                                       TCGCGGCCTTTGGTGTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGT                                            <  SRR19973069.1624126/150‑1 (MQ=60)
                                                                                                          CGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATC                                         <  SRR19973069.3665405/150‑1 (MQ=60)
                                                                                                          CGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATC                                         <  SRR19973069.3665388/150‑1 (MQ=60)
                                                                                                          CGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATC                                         <  SRR19973069.926549/150‑1 (MQ=60)
                                                                                                          CGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATC                                         <  SRR19973069.926690/150‑1 (MQ=60)
                                                                                                           GGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCT                                        <  SRR19973069.601073/150‑1 (MQ=60)
                                                                                                              CTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGA                                     >  SRR19973069.716612/1‑150 (MQ=60)
                                                                                                              CTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGA                                     >  SRR19973069.773896/1‑150 (MQ=60)
                                                                                                               gatctTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAC                                    <  SRR19973069.958016/145‑1 (MQ=60)
                                                                                                               gatctTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAC                                    <  SRR19973069.958108/145‑1 (MQ=60)
                                                                                                                   GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTC                                >  SRR19973069.1338402/1‑150 (MQ=60)
                                                                                                                   GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTC                                >  SRR19973069.2179021/1‑150 (MQ=60)
                                                                                                                   GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTC                                >  SRR19973069.2179094/1‑150 (MQ=60)
                                                                                                                             CGGCGCTTACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGTTAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACACTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCG                      <  SRR19973069.1424915/150‑1 (MQ=60)
                                                                                                                               GCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTT                    <  SRR19973069.3565961/150‑1 (MQ=60)
                                                                                                                                CGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTT                   <  SRR19973069.2291883/150‑1 (MQ=60)
                                                                                                                                CGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTT                   <  SRR19973069.2292057/150‑1 (MQ=60)
                                                                                                                                CGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTGT                   >  SRR19973069.1798105/1‑150 (MQ=60)
                                                                                                                                  CTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGACATGTATCTTGACGTTCTGGCCAGTCGTGTGG                 >  SRR19973069.2108778/1‑150 (MQ=60)
                                                                                                                                  CTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGG                 <  SRR19973069.3233812/150‑1 (MQ=60)
                                                                                                                                  CTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGG                 <  SRR19973069.3233793/150‑1 (MQ=60)
                                                                                                                                       GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            >  SRR19973069.401422/1‑150 (MQ=60)
                                                                                                                                       GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            >  SRR19973069.3780913/1‑150 (MQ=60)
                                                                                                                                       GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            >  SRR19973069.1876260/1‑150 (MQ=60)
                                                                                                                                       GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            <  SRR19973069.3466596/150‑1 (MQ=60)
                                                                                                                                       GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTGTGGGGATG            >  SRR19973069.3357445/1‑150 (MQ=60)
                                                                                                                                       GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACACTGTTGAGTGATTTTAAGTGACAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCAAGTCGTTTGTGGATG            >  SRR19973069.2386888/1‑150 (MQ=60)
                                                                                                                                           CCGTGCTTCTGGCATACCTTGAATCGCGGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATG        <  SRR19973069.1126537/150‑1 (MQ=60)
                                                                                                                                           CCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATG        <  SRR19973069.3449612/150‑1 (MQ=60)
                                                                                                                                           CCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATG        <  SRR19973069.1126514/150‑1 (MQ=60)
                                                                                                                                                CTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCT   >  SRR19973069.2242547/1‑150 (MQ=60)
                                                                                                                                                 TTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  <  SRR19973069.1549275/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
GTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  >  CP009273/253710‑254004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑