Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 253,855 G→T 100% R281L (CGT→CTT)  frsA → fermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273253,8550GT99.0% 976.9 / 0.2 301R281L (CGT→CTT) frsAfermentation‑respiration switch protein; PTS Enzyme IIA(Glc)‑binding protein; pNP‑butyrate esterase activity
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (132/166);  total (134/167)
Fisher's exact test for biased strand distribution p-value = 5.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 301 total aligned reads displayed.

GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATG  >  CP009273/253709‑254001
                                                                                                                                                  |                                                                                                                                                  
ggTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCt                                                                                                                                                 >  1:728156/1‑150 (MQ=255)
  tGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTgg                                                                                                                                               >  1:4257790/1‑150 (MQ=255)
   gggCTTGTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGc                                                                                                                                              <  1:1126683/150‑1 (MQ=255)
    ggCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCa                                                                                                                                             >  2:3940641/1‑150 (MQ=255)
         ttcttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCt                                                                                                                                        <  2:1202197/150‑1 (MQ=255)
          tcttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACGGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCtt                                                                                                                                       <  2:5000222/150‑1 (MQ=255)
           cttcAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTg                                                                                                                                      <  2:4433900/150‑1 (MQ=255)
                aaaTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcg                                                                                                                                 <  2:3876040/150‑1 (MQ=255)
                   tGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcgccg                                                                                                                              >  1:2674890/1‑150 (MQ=255)
                    ggAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATcgccgc                                                                                                                             >  1:851538/1‑150 (MQ=255)
                       aGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTc                                                                                                                          >  2:202224/1‑150 (MQ=255)
                       aGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGACGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTc                                                                                                                          >  1:3003080/1‑150 (MQ=255)
                        gCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCt                                                                                                                         <  2:2550850/150‑1 (MQ=255)
                         cTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTg                                                                                                                        >  2:550767/1‑150 (MQ=255)
                         cTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTg                                                                                                                        <  2:5236461/150‑1 (MQ=255)
                         cTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTg                                                                                                                        >  2:4652634/1‑150 (MQ=255)
                             cccAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAg                                                                                                                    >  2:5274118/1‑150 (MQ=255)
                               cAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCg                                                                                                                  >  1:4663495/1‑150 (MQ=255)
                               cAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCg                                                                                                                  >  1:4733345/1‑150 (MQ=255)
                               cAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCGTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCg                                                                                                                  >  1:2289305/1‑150 (MQ=255)
                                aGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCgg                                                                                                                 >  2:4251150/1‑150 (MQ=255)
                                  gACTCCAGCCTGTTGCATCAGCACGTCTTACAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGtt                                                                                                               <  2:943084/150‑1 (MQ=255)
                                  gACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGtt                                                                                                               <  2:3548700/150‑1 (MQ=255)
                                   aCTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTg                                                                                                              <  1:1756739/150‑1 (MQ=255)
                                    cTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGACTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGc                                                                                                             >  2:28288/1‑150 (MQ=255)
                                     tCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGcc                                                                                                            <  1:3723395/150‑1 (MQ=255)
                                      ccAGCCTGGTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCt                                                                                                           <  2:1458579/150‑1 (MQ=255)
                                      ccAGCCTGGTGCATCAGCACGTCTTAAAGGCGCGGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTGTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCt                                                                                                           <  2:1952209/150‑1 (MQ=255)
                                       cAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTg                                                                                                          >  1:459375/1‑150 (MQ=255)
                                        aGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGt                                                                                                         >  1:2019274/1‑150 (MQ=255)
                                           cTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCtt                                                                                                      >  1:421988/1‑150 (MQ=255)
                                           cTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCtt                                                                                                      <  2:890698/150‑1 (MQ=255)
                                           cTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCtt                                                                                                      <  2:1755986/150‑1 (MQ=255)
                                           cTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCtt                                                                                                      <  2:1546551/150‑1 (MQ=255)
                                            tGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                     >  2:2370033/1‑150 (MQ=255)
                                            tGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTg                                                                                                     <  2:1129316/150‑1 (MQ=255)
                                             gTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTgg                                                                                                    >  1:3110797/1‑150 (MQ=255)
                                             gTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTgg                                                                                                    >  1:339290/1‑150 (MQ=255)
                                              ttGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGt                                                                                                   >  1:4748091/1‑150 (MQ=255)
                                                gCATCAGCACGTCTTACAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTcc                                                                                                 <  2:751815/150‑1 (MQ=255)
                                                gCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTcc                                                                                                 >  1:5026924/1‑150 (MQ=255)
                                                  aTCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCgg                                                                                               >  1:1636539/1‑150 (MQ=255)
                                                   tCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGt                                                                                              <  2:4971872/150‑1 (MQ=255)
                                                    cAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTa                                                                                             <  1:4494281/150‑1 (MQ=255)
                                                     aGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAg                                                                                            <  1:2420750/150‑1 (MQ=255)
                                                     aGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCGTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAg                                                                                            >  1:1856698/1‑150 (MQ=255)
                                                      gCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGt                                                                                           <  1:4820163/150‑1 (MQ=255)
                                                        aCGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTc                                                                                         <  1:3939728/150‑1 (MQ=255)
                                                         cGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCa                                                                                        <  1:1974061/150‑1 (MQ=255)
                                                          gTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCAt                                                                                       <  1:2218079/150‑1 (MQ=255)
                                                           tCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATa                                                                                      <  2:4572639/150‑1 (MQ=255)
                                                                aaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                                 <  2:507858/150‑1 (MQ=255)
                                                                aaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                                 <  2:3113698/150‑1 (MQ=255)
                                                                aaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                                 >  1:1884605/1‑150 (MQ=255)
                                                                aaGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTg                                                                                 <  1:3413482/150‑1 (MQ=255)
                                                                      cTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGt                                                                           <  1:3783577/150‑1 (MQ=255)
                                                                         ccTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAt                                                                        <  1:5148802/150‑1 (MQ=255)
                                                                          cTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtt                                                                       <  1:1348848/150‑1 (MQ=255)
                                                                           tAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAttt                                                                      >  1:231755/1‑150 (MQ=255)
                                                                            aaCGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtttt                                                                     <  2:1590585/150‑1 (MQ=255)
                                                                            aaCGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAtttt                                                                     <  2:164490/150‑1 (MQ=255)
                                                                                   cGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGcc                                                              >  2:4198118/1‑150 (MQ=255)
                                                                                     tgggtggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGACAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAg                                                            >  2:1897606/1‑150 (MQ=255)
                                                                                          ggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACa                                                       >  2:2450526/1‑150 (MQ=255)
                                                                                          ggATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACa                                                       >  2:3716783/1‑150 (MQ=255)
                                                                                            aTCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAgg                                                     <  2:3435332/150‑1 (MQ=255)
                                                                                            aTCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAgg                                                     >  2:411920/1‑150 (MQ=255)
                                                                                              cacaCTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTg                                                   <  1:2370033/150‑1 (MQ=255)
                                                                                              cacaCTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTg                                                   >  2:3786394/1‑150 (MQ=255)
                                                                                               acacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGc                                                  <  2:2167012/150‑1 (MQ=255)
                                                                                               acacTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGc                                                  <  2:4816803/150‑1 (MQ=255)
                                                                                                 acTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCg                                                <  1:48661/150‑1 (MQ=255)
                                                                                                 acTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCg                                                <  1:2739123/150‑1 (MQ=255)
                                                                                                 acTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCg                                                <  2:603993/150‑1 (MQ=255)
                                                                                                        tcgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGt                                         <  1:1504857/150‑1 (MQ=255)
                                                                                                         cgcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTa                                        >  2:3832469/1‑150 (MQ=255)
                                                                                                          gcgGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTAt                                       <  2:3218853/150‑1 (MQ=255)
                                                                                                            ggCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCt                                     >  2:3758896/1‑150 (MQ=255)
                                                                                                            ggCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCt                                     >  1:781459/1‑150 (MQ=255)
                                                                                                              ccTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTg                                   <  1:584353/150‑1 (MQ=255)
                                                                                                                tttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAc                                 <  2:2674890/150‑1 (MQ=255)
                                                                                                                tttGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGAc                                 <  2:4221182/150‑1 (MQ=255)
                                                                                                                   ggTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGtt                              <  2:1242885/150‑1 (MQ=255)
                                                                                                                    gTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTc                             >  2:2248535/1‑150 (MQ=255)
                                                                                                                     tttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCt                            <  1:5311702/150‑1 (MQ=255)
                                                                                                                      ttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTg                           <  1:1022855/150‑1 (MQ=255)
                                                                                                                      ttCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTg                           <  1:3328702/150‑1 (MQ=255)
                                                                                                                         cGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGcc                        >  2:1099346/1‑150 (MQ=255)
                                                                                                                               ggCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGt                  >  1:3157600/1‑150 (MQ=255)
                                                                                                                                gcgcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGtt                 <  1:4225046/150‑1 (MQ=255)
                                                                                                                                  gcTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGttta               <  2:4351098/150‑2 (MQ=255)
                                                                                                                                   cTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTgg              >  2:4032621/1‑150 (MQ=255)
                                                                                                                                    tAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGACATGTATCTTGACGTTCTGGCCAGTCGTGTggg             >  1:3596815/1‑150 (MQ=255)
                                                                                                                                     aaCGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTgggg            <  2:3003080/150‑1 (MQ=255)
                                                                                                                                     aaCGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTgggg            >  2:1016440/1‑150 (MQ=255)
                                                                                                                                          tGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCa       >  1:3062067/1‑150 (MQ=255)
                                                                                                                                           gCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCat      >  2:359910/1‑150 (MQ=255)
                                                                                                                                           gCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCat      <  2:1359966/150‑1 (MQ=255)
                                                                                                                                              gTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTTGGGATTCatgat   >  1:1191056/1‑150 (MQ=255)
                                                                                                                                              gTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCatgat   <  1:5208936/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATG  >  CP009273/253709‑254001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  >  CP009273/253706‑254004
                                                                                                                                                     |                                                                                                                                                     
GTCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCT                                                                                                                                                       >  SRR19973062.2338677/1‑150 (MQ=60)
 TCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTT                                                                                                                                                      <  SRR19973062.405382/150‑1 (MQ=60)
   GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCT                                                                                                                                                    >  SRR19973062.731476/1‑150 (MQ=60)
   GGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCT                                                                                                                                                    <  SRR19973062.1790921/150‑1 (MQ=60)
     TGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGG                                                                                                                                                  >  SRR19973062.4279843/1‑150 (MQ=60)
      GGGCTTGTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGC                                                                                                                                                 <  SRR19973062.1131964/150‑1 (MQ=60)
         CTTTTCTTCAAACTGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATA                                                                                                                                              <  SRR19973062.905105/150‑1 (MQ=60)
         CTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATA                                                                                                                                              >  SRR19973062.131374/1‑150 (MQ=60)
         CTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATA                                                                                                                                              <  SRR19973062.421128/150‑1 (MQ=60)
             TCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTT                                                                                                                                          <  SRR19973062.1039101/150‑1 (MQ=60)
             TCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTT                                                                                                                                          >  SRR19973062.54060/1‑150 (MQ=60)
               TTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGA                                                                                                                                        <  SRR19973062.3800793/150‑1 (MQ=60)
                  AAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATC                                                                                                                                     <  SRR19973062.304012/150‑1 (MQ=60)
                   AAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCG                                                                                                                                    <  SRR19973062.3085578/150‑1 (MQ=60)
                     ATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCC                                                                                                                                  >  SRR19973062.5137719/1‑150 (MQ=60)
                      TGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCG                                                                                                                                 >  SRR19973062.2688908/1‑150 (MQ=60)
                       GGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGC                                                                                                                                >  SRR19973062.855218/1‑150 (MQ=60)
                         AAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGT                                                                                                                              >  SRR19973062.2090723/1‑150 (MQ=60)
                          AGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTC                                                                                                                             <  SRR19973062.4888763/150‑1 (MQ=60)
                          AGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGACGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTC                                                                                                                             >  SRR19973062.3018782/1‑150 (MQ=60)
                           GCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCT                                                                                                                            <  SRR19973062.4807508/150‑1 (MQ=60)
                             TCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGA                                                                                                                          >  SRR19973062.5186060/1‑150 (MQ=60)
                             TCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGA                                                                                                                          <  SRR19973062.4624480/150‑1 (MQ=60)
                              CACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAA                                                                                                                         >  SRR19973062.3160410/1‑150 (MQ=60)
                               ACCCAGGACTCCGGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAA                                                                                                                        >  SRR19973062.1408723/1‑150 (MQ=60)
                                  CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                     >  SRR19973062.4758077/1‑150 (MQ=60)
                                  CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                     >  SRR19973062.4758027/1‑150 (MQ=60)
                                  CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                     >  SRR19973062.4687894/1‑150 (MQ=60)
                                  CAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCGTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCG                                                                                                                     >  SRR19973062.2301161/1‑150 (MQ=60)
                                   AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGG                                                                                                                    >  SRR19973062.753095/1‑150 (MQ=60)
                                   AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGG                                                                                                                    >  SRR19973062.2297961/1‑150 (MQ=60)
                                   AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGG                                                                                                                    >  SRR19973062.1149888/1‑150 (MQ=60)
                                   AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGG                                                                                                                    >  SRR19973062.698301/1‑150 (MQ=60)
                                   AGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGG                                                                                                                    >  SRR19973062.4537199/1‑150 (MQ=60)
                                      ACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTG                                                                                                                 <  SRR19973062.1765261/150‑1 (MQ=60)
                                       CTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGC                                                                                                                <  SRR19973062.1714212/150‑1 (MQ=60)
                                        TCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCC                                                                                                               <  SRR19973062.3742502/150‑1 (MQ=60)
                                        TCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCC                                                                                                               <  SRR19973062.760734/150‑1 (MQ=60)
                                          CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             >  SRR19973062.461377/1‑150 (MQ=60)
                                          CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             >  SRR19973062.461386/1‑150 (MQ=60)
                                          CAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTG                                                                                                             >  SRR19973062.502889/1‑150 (MQ=60)
                                           AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGT                                                                                                            >  SRR19973062.2029658/1‑150 (MQ=60)
                                           AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGT                                                                                                            >  SRR19973062.2365200/1‑150 (MQ=60)
                                           AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGT                                                                                                            >  SRR19973062.3736112/1‑150 (MQ=60)
                                           AGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGT                                                                                                            >  SRR19973062.3736132/1‑150 (MQ=60)
                                              CTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTT                                                                                                         >  SRR19973062.423854/1‑150 (MQ=60)
                                              CTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTT                                                                                                         >  SRR19973062.423805/1‑150 (MQ=60)
                                               TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                        <  SRR19973062.3414167/150‑1 (MQ=60)
                                               TGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTG                                                                                                        >  SRR19973062.189247/1‑150 (MQ=60)
                                                GTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGG                                                                                                       >  SRR19973062.340852/1‑150 (MQ=60)
                                                GTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGG                                                                                                       >  SRR19973062.3126814/1‑150 (MQ=60)
                                                GTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGG                                                                                                       >  SRR19973062.340863/1‑150 (MQ=60)
                                                 TTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGT                                                                                                      >  SRR19973062.4772814/1‑150 (MQ=60)
                                                   GCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCC                                                                                                    <  SRR19973062.2737685/150‑1 (MQ=60)
                                                   GCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCC                                                                                                    >  SRR19973062.5053557/1‑150 (MQ=60)
                                                     ATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGG                                                                                                  >  SRR19973062.728332/1‑150 (MQ=60)
                                                     ATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGG                                                                                                  >  SRR19973062.1644610/1‑150 (MQ=60)
                                                       CAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTA                                                                                                <  SRR19973062.4517483/150‑1 (MQ=60)
                                                       CAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTA                                                                                                >  SRR19973062.4688636/1‑150 (MQ=60)
                                                       CAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTA                                                                                                <  SRR19973062.479158/150‑1 (MQ=60)
                                                        AGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAG                                                                                               <  SRR19973062.2433755/150‑1 (MQ=60)
                                                        AGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCGTGGTCCGGTAG                                                                                               >  SRR19973062.1866062/1‑150 (MQ=60)
                                                        AGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCGTGAATCGCCGCGTCTGAAAGCGGTTGCCGGTCTTGGTCCGGTAG                                                                                               >  SRR19973062.1866001/1‑150 (MQ=60)
                                                         GCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGT                                                                                              <  SRR19973062.4845117/150‑1 (MQ=60)
                                                           ACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTC                                                                                            >  SRR19973062.2708566/1‑150 (MQ=60)
                                                           ACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTC                                                                                            <  SRR19973062.3959729/150‑1 (MQ=60)
                                                            CGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCA                                                                                           <  SRR19973062.1984213/150‑1 (MQ=60)
                                                            CGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCA                                                                                           >  SRR19973062.4248812/1‑150 (MQ=60)
                                                             GTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCAT                                                                                          <  SRR19973062.2229192/150‑1 (MQ=60)
                                                                TTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACC                                                                                       <  SRR19973062.4876800/150‑1 (MQ=60)
                                                                   AAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTG                                                                                    >  SRR19973062.1894170/1‑150 (MQ=60)
                                                                   AAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTG                                                                                    <  SRR19973062.3430958/150‑1 (MQ=60)
                                                                   AAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTG                                                                                    <  SRR19973062.3614275/150‑1 (MQ=60)
                                                                     GGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTT                                                                                  >  SRR19973062.3322435/1‑150 (MQ=60)
                                                                       CGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGA                                                                                >  SRR19973062.5216032/1‑150 (MQ=60)
                                                                         CTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGT                                                                              <  SRR19973062.3802956/150‑1 (MQ=60)
                                                                            CCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGAT                                                                           <  SRR19973062.5176017/150‑1 (MQ=60)
                                                                             CTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATT                                                                          <  SRR19973062.1354942/150‑1 (MQ=60)
                                                                             CTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCACGTCCGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATT                                                                          <  SRR19973062.3846179/150‑1 (MQ=60)
                                                                              TAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTT                                                                         >  SRR19973062.232948/1‑150 (MQ=60)
                                                                              TAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTT                                                                         <  SRR19973062.2290102/150‑1 (MQ=60)
                                                                                  GTACCGTGGGTGGATCACACTCGCGTCGCGGCCGTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAG                                                                     >  SRR19973062.1340088/1‑150 (MQ=60)
                                                                                   TACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGT                                                                    <  SRR19973062.1464283/150‑1 (MQ=60)
                                                                                    ACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTG                                                                   <  SRR19973062.5106444/150‑1 (MQ=60)
                                                                                       GTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCA                                                                <  SRR19973062.2351375/150‑1 (MQ=60)
                                                                                         GGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGC                                                              >  SRR19973062.2402265/1‑150 (MQ=60)
                                                                                            TGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGATTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAAC                                                           <  SRR19973062.1802818/150‑1 (MQ=60)
                                                                                              GATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAG                                                         >  SRR19973062.879727/1‑150 (MQ=60)
                                                                                               ATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGG                                                        >  SRR19973062.5333396/1‑150 (MQ=60)
                                                                                                TCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGT                                                       <  SRR19973062.4698967/150‑1 (MQ=60)
                                                                                                 CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      <  SRR19973062.2382717/150‑1 (MQ=60)
                                                                                                 CACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTG                                                      <  SRR19973062.3362865/150‑1 (MQ=60)
                                                                                                   CACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCA                                                    <  SRR19973062.4655372/150‑1 (MQ=60)
                                                                                                    ACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCG                                                   <  SRR19973062.2753565/150‑1 (MQ=60)
                                                                                                    ACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCG                                                   <  SRR19973062.49014/150‑1 (MQ=60)
                                                                                                     CTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGG                                                  <  SRR19973062.426440/150‑1 (MQ=60)
                                                                                                     CTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCGGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGG                                                  >  SRR19973062.4933916/1‑150 (MQ=60)
                                                                                                         CGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAAT                                              <  SRR19973062.5342019/150‑1 (MQ=60)
                                                                                                          GTCGCGGCCTTTGGTTTCCGTTTCGGCGCTACCGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATG                                             <  SRR19973062.3461494/150‑1 (MQ=60)
                                                                                                           TCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGT                                            <  SRR19973062.1512257/150‑1 (MQ=60)
                                                                                                              CGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATC                                         >  SRR19973062.798701/1‑150 (MQ=60)
                                                                                                              CGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATC                                         <  SRR19973062.4471454/150‑1 (MQ=60)
                                                                                                               GGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCT                                        >  SRR19973062.784944/1‑150 (MQ=60)
                                                                                                               GGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCT                                        >  SRR19973062.784989/1‑150 (MQ=60)
                                                                                                                GCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTT                                       <  SRR19973062.3202770/150‑1 (MQ=60)
                                                                                                                 CCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTG                                      <  SRR19973062.587081/150‑1 (MQ=60)
                                                                                                                 CCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTG                                      >  SRR19973062.2027876/1‑150 (MQ=60)
                                                                                                                 CCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTG                                      >  SRR19973062.2028141/1‑150 (MQ=60)
                                                                                                                  CTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGA                                     >  SRR19973062.5336391/1‑150 (MQ=60)
                                                                                                                       GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTC                                >  SRR19973062.4406691/1‑150 (MQ=60)
                                                                                                                       GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTC                                >  SRR19973062.230563/1‑150 (MQ=60)
                                                                                                                       GTTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGATC                                >  SRR19973062.230507/1‑150 (MQ=60)
                                                                                                                        TTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCT                               <  SRR19973062.1117965/150‑1 (MQ=60)
                                                                                                                        TTTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCT                               <  SRR19973062.5339583/150‑1 (MQ=60)
                                                                                                                         TTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTG                              <  SRR19973062.1153143/150‑1 (MQ=60)
                                                                                                                         TTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTG                              <  SRR19973062.2422217/150‑1 (MQ=60)
                                                                                                                         TTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTG                              <  SRR19973062.3345892/150‑1 (MQ=60)
                                                                                                                         TTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTG                              <  SRR19973062.3742556/150‑1 (MQ=60)
                                                                                                                         TTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTG                              <  SRR19973062.3051627/150‑1 (MQ=60)
                                                                                                                         TTCCGTTTCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTG                              <  SRR19973062.1027652/150‑1 (MQ=60)
                                                                                                                                TCGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTC                       >  SRR19973062.1929723/1‑150 (MQ=60)
                                                                                                                                 CGGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCG                      <  SRR19973062.1670949/150‑1 (MQ=60)
                                                                                                                                  GGCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGT                     >  SRR19973062.3173919/1‑150 (MQ=60)
                                                                                                                                   GCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTT                    <  SRR19973062.4246909/150‑1 (MQ=60)
                                                                                                                                   GCGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTT                    <  SRR19973062.4246947/150‑1 (MQ=60)
                                                                                                                                    CGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTT                   <  SRR19973062.1602150/150‑1 (MQ=60)
                                                                                                                                    CGCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTT                   <  SRR19973062.1602107/150‑1 (MQ=60)
                                                                                                                                     GCTAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTG                  >  SRR19973062.383520/1‑150 (MQ=60)
                                                                                                                                       TAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGACATGTATCTTGACGTTCTGGCCAGTCGTGTGGG                >  SRR19973062.3615079/1‑150 (MQ=60)
                                                                                                                                       TAACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGG                >  SRR19973062.3173327/1‑150 (MQ=60)
                                                                                                                                        AACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGG               >  SRR19973062.2665736/1‑150 (MQ=60)
                                                                                                                                         ACGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGA              <  SRR19973062.3960646/150‑1 (MQ=60)
                                                                                                                                          CGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGAT             <  SRR19973062.2516798/150‑1 (MQ=60)
                                                                                                                                          CGTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGAT             <  SRR19973062.2891576/150‑1 (MQ=60)
                                                                                                                                           GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            <  SRR19973062.2132920/150‑1 (MQ=60)
                                                                                                                                           GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            >  SRR19973062.1931473/1‑150 (MQ=60)
                                                                                                                                           GTTGCCGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATG            >  SRR19973062.4772780/1‑150 (MQ=60)
                                                                                                                                             TGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCA          >  SRR19973062.3077998/1‑150 (MQ=60)
                                                                                                                                             TGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCA          >  SRR19973062.3077951/1‑150 (MQ=60)
                                                                                                                                                CGTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGA       >  SRR19973062.3370575/1‑150 (MQ=60)
                                                                                                                                                 GTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTTGGGATTCATGAT      >  SRR19973062.1196623/1‑150 (MQ=60)
                                                                                                                                                 GTGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGAT      <  SRR19973062.5236413/150‑1 (MQ=60)
                                                                                                                                                  TGCTTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATG     <  SRR19973062.2761764/150‑1 (MQ=60)
                                                                                                                                                   GCTTCTGACATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGC    >  SRR19973062.993689/1‑150 (MQ=60)
                                                                                                                                                     TTCTGGCATCCCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTATGGCCAGTCGTTTGGGGATGCATGATGCTT  <  SRR19973062.5007709/150‑1 (MQ=60)
                                                                                                                                                     TTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  <  SRR19973062.2479635/150‑1 (MQ=60)
                                                                                                                                                     TTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTGTGGGGATGCATGATGCTT  >  SRR19973062.1608400/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GTCGGTGGGCTTTTCTTCAAAATGGAAGCTCACCCAGGACTCCAGCCTGTTGCATCAGCACGTCTTAAAGGCGCTGCCTAACGTACCGTGGGTGGATCACACTCGCGTCGCGGCCTTTGGTTTCCGTTTCGGCGCTAACGTTGCCGTGCGTCTGGCATACCTTGAATCGCCGCGTCTGAAAGCGGTTGCCTGTCTTGGTCCGGTAGTTCATACCCTGTTGAGTGATTTTAAGTGCCAGCAACAGGTGCCGGAAATGTATCTTGACGTTCTGGCCAGTCGTTTGGGGATGCATGATGCTT  >  CP009273/253706‑254004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑