Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,540,399 A→G 47.3% intergenic (‑114/+146) yddL ← / ← yddG putative lipoprotein;putative membrane; Not classified; putative outer membrane porin protein/aromatic amino acid exporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,540,3990AG47.3% 30.7 / inf 296intergenic (‑114/+146)yddL/yddGputative lipoprotein;putative membrane; Not classified; putative outer membrane porin protein/aromatic amino acid exporter
Reads supporting (aligned to +/- strand):  ref base A (58/98);  new base G (53/87);  total (111/185)
Fisher's exact test for biased strand distribution p-value = 9.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 308 total aligned reads displayed.

CAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTAACTACCGGGCCGTAGACCCGGCAGTTATTTT  >  CP009273/1540252‑1540546
                                                                                                                                                   |                                                                                                                                                   
cAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATgaga                                                                                                                                                     <  2:3362400/148‑1 (MQ=255)
     aCAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAAt                                                                                                                                                <  1:2572015/148‑1 (MQ=255)
       aaCCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAg                                                                                                                                              <  2:3517382/148‑1 (MQ=255)
        accaccGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGa                                                                                                                                             <  2:2524343/148‑1 (MQ=255)
         ccaccGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGAt                                                                                                                                            >  1:1644042/1‑148 (MQ=255)
            ccGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGaa                                                                                                                                         <  2:169292/148‑1 (MQ=255)
            ccGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGaa                                                                                                                                         >  2:3021560/1‑148 (MQ=255)
            ccGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGaa                                                                                                                                         >  2:1440686/1‑148 (MQ=255)
             cGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGaaa                                                                                                                                        <  2:658320/148‑1 (MQ=255)
             cGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGaaa                                                                                                                                        >  2:1673092/1‑148 (MQ=255)
                aaCTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAaataa                                                                                                                                     <  1:1968814/148‑1 (MQ=255)
                  cTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAataata                                                                                                                                   >  2:2785276/1‑148 (MQ=255)
                  cTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAataata                                                                                                                                   >  2:1605488/1‑148 (MQ=255)
                  cTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAataata                                                                                                                                   >  1:3621954/1‑148 (MQ=255)
                    aTTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAACATAGATGAAATAAtatt                                                                                                                                 >  2:2944955/1‑148 (MQ=255)
                    aTTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAAtatt                                                                                                                                 >  1:208267/1‑148 (MQ=255)
                     tttttAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAAtatta                                                                                                                                <  2:1778250/148‑1 (MQ=255)
                       tttAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATattatt                                                                                                                              >  2:473716/1‑148 (MQ=255)
                        ttAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATAttattt                                                                                                                             <  1:1497243/148‑1 (MQ=255)
                        ttAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATAttattt                                                                                                                             <  2:3604291/148‑1 (MQ=255)
                        ttAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATAttattt                                                                                                                             >  2:519618/1‑148 (MQ=255)
                        ttAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATAttattt                                                                                                                             <  2:1809777/148‑1 (MQ=255)
                          aaTTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTACACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATAttatttat                                                                                                                           <  1:2269867/148‑1 (MQ=255)
                            tttCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCg                                                                                                                         >  2:1357232/1‑148 (MQ=255)
                             ttCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGa                                                                                                                        >  1:1413931/1‑148 (MQ=255)
                             ttCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGa                                                                                                                        >  1:370683/1‑148 (MQ=255)
                                    aaCAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGa                                                                                                                 <  1:2314769/148‑1 (MQ=255)
                                    aaCAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGa                                                                                                                 <  1:391924/148‑1 (MQ=255)
                                      cAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATc                                                                                                               >  2:3127675/1‑148 (MQ=255)
                                       aGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCg                                                                                                              >  2:222435/1‑148 (MQ=255)
                                        gAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGa                                                                                                             >  2:3151385/1‑148 (MQ=255)
                                        gAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGa                                                                                                             >  1:2472510/1‑148 (MQ=255)
                                         aaTCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGaa                                                                                                            >  1:2063536/1‑148 (MQ=255)
                                           tCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGt                                                                                                          >  1:1453723/1‑148 (MQ=255)
                                              ttttAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGa                                                                                                       <  1:103230/148‑1 (MQ=255)
                                                   aTTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAACAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATga                                                                                                  >  1:747576/1‑148 (MQ=255)
                                                    ttATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATgag                                                                                                 <  1:1754270/148‑1 (MQ=255)
                                                        cGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAAGGAGatct                                                                                             >  2:2676429/1‑146 (MQ=255)
                                                         gTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGAtata                                                                                            <  1:1250430/148‑1 (MQ=255)
                                                          ttAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATaa                                                                                           <  2:1122271/148‑1 (MQ=255)
                                                           tAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAg                                                                                          <  1:139373/148‑1 (MQ=255)
                                                           tAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAg                                                                                          >  1:3459725/1‑148 (MQ=255)
                                                           tAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAg                                                                                          >  1:3327281/1‑148 (MQ=255)
                                                            aaaCGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAgg                                                                                         >  1:616665/1‑148 (MQ=255)
                                                             aaCGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGt                                                                                        <  1:3377213/148‑1 (MQ=255)
                                                              aCGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTg                                                                                       >  1:1896118/1‑148 (MQ=255)
                                                                 tATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAAt                                                                                    <  2:807029/148‑1 (MQ=255)
                                                                    tttCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTAc                                                                                 <  2:149181/148‑1 (MQ=255)
                                                                          aaCGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTAt                                                                           <  2:1515254/148‑1 (MQ=255)
                                                                               aTTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGaa                                                                      <  2:1529432/148‑1 (MQ=255)
                                                                                 tttAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAat                                                                    >  1:310574/1‑148 (MQ=255)
                                                                                   tAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAattt                                                                  <  1:1861525/148‑1 (MQ=255)
                                                                                       cGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAatttattt                                                              <  2:1176662/148‑1 (MQ=255)
                                                                                       cGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAatttattt                                                              <  1:286749/148‑1 (MQ=255)
                                                                                        ggCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTAtttt                                                             <  2:1106760/148‑1 (MQ=255)
                                                                                        ggCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTAtttt                                                             <  1:3058677/148‑1 (MQ=255)
                                                                                         gCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTAttttt                                                            <  1:990663/148‑1 (MQ=255)
                                                                                         gCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTAttttt                                                            <  2:3804508/148‑1 (MQ=255)
                                                                                          cGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTAtttttt                                                           >  2:258741/1‑148 (MQ=255)
                                                                                            tCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTa                                                         <  2:3063634/148‑1 (MQ=255)
                                                                                            tCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTa                                                         <  2:2383525/148‑1 (MQ=255)
                                                                                             cATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTaa                                                        >  2:1400523/1‑148 (MQ=255)
                                                                                             cATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTaa                                                        >  1:858869/1‑148 (MQ=255)
                                                                                              aTTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAat                                                       <  1:2785276/148‑1 (MQ=255)
                                                                                              aTTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAat                                                       <  1:1605488/148‑1 (MQ=255)
                                                                                              aTTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAat                                                       <  2:370683/148‑1 (MQ=255)
                                                                                              aTTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAat                                                       <  2:2198406/148‑1 (MQ=255)
                                                                                               tttATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAata                                                      <  1:1202545/148‑1 (MQ=255)
                                                                                               tttATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGAAGAAATAATATAAATTATCGATATGTGATCGAAGTCGAAATGAGATATAGGGTGAATTACTGGTATTTGAAATATATTTTTTTAata                                                      >  1:117569/1‑148 (MQ=255)
                                                                                                 tataAATGACATACTTTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATAtt                                                    <  2:2473181/148‑1 (MQ=255)
                                                                                                 tataAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATAtt                                                    <  1:3547223/148‑1 (MQ=255)
                                                                                                  ataAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTg                                                   <  2:1217402/148‑1 (MQ=255)
                                                                                                   taAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGt                                                  >  2:1900051/1‑148 (MQ=255)
                                                                                                     aaTGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCg                                                >  2:101206/1‑148 (MQ=255)
                                                                                                      aTGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCgg                                               >  1:1132664/1‑148 (MQ=255)
                                                                                                       tGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGa                                              <  1:334272/148‑1 (MQ=255)
                                                                                                        gACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGaa                                             <  1:2691274/148‑1 (MQ=255)
                                                                                                         aCATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAAt                                            <  1:455789/148‑1 (MQ=255)
                                                                                                         aCATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAAt                                            <  2:3251021/148‑1 (MQ=255)
                                                                                                          cATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAAtt                                           <  1:1357232/148‑1 (MQ=255)
                                                                                                          cATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAAtt                                           <  1:3260553/148‑1 (MQ=255)
                                                                                                           aTACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCTAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAAttt                                          <  2:1142948/148‑1 (MQ=255)
                                                                                                           aTACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAAttt                                          <  2:437171/148‑1 (MQ=255)
                                                                                                             actcattttAAAATAGAATGGTGATTAAAAAAAATGAGAAAAATAGATTTAATAAGATTATTTATCGATTTGTGATCTAATTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTAt                                        <  2:2856744/143‑1 (MQ=255)
                                                                                                             aCTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTAt                                        <  2:1621706/148‑1 (MQ=255)
                                                                                                             aCTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTAt                                        >  2:1284176/1‑148 (MQ=255)
                                                                                                             aCTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTAt                                        >  2:3327711/1‑148 (MQ=255)
                                                                                                             aCTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTAt                                        <  2:1621522/148‑1 (MQ=255)
                                                                                                             aCTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTAt                                        >  1:591706/1‑148 (MQ=255)
                                                                                                              cTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATc                                       <  2:596718/148‑1 (MQ=255)
                                                                                                              cTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATc                                       <  2:3459725/148‑1 (MQ=255)
                                                                                                               tGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGGAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTAGCt                                      >  1:3121941/1‑148 (MQ=255)
                                                                                                                  ttttAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGAt                                   <  1:156028/148‑1 (MQ=255)
                                                                                                                     tAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTaa                                <  2:2469967/148‑1 (MQ=255)
                                                                                                                                 gTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTAACTACCGGGCCGt                    <  1:3139530/148‑1 (MQ=255)
                                                                                                                                    aTTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTAACTACCGGGCCGTAGa                 >  1:3524036/1‑148 (MQ=255)
                                                                                                                                         aaaCAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTAACTACCGGGCCGTAGACCCgg            >  2:2266687/1‑148 (MQ=255)
                                                                                                                                          aaCAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTAACTACCGGGCCGTAGACCCGGc           <  2:40947/148‑1 (MQ=255)
                                                                                                                                              aTGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTAACTACCGGGCCGTAGACCCGGCAGtt       <  2:20530/148‑1 (MQ=255)
                                                                                                                                                  gaAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTAACTACCGGGCCGTAGACCCGGCAGTTAttt   >  1:657236/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGTTACAACCACCGCAACTATTTTTAATTTCATTTAACAGAATCCTTTTAATTATCGTTAAACGTATTTTCTAAACGAATTTTAAACGGCGTCATTTATAAATGACATACTGTTTTTAAAATAGAATGGTGATTAAAAACAATGAGAAAAATAGATGAAATAATATTATTTATCGATATGTGATCGAAGTCGAAATGAGATATAAGGTGAATTACTGGTATTTGAAATTTATTTTTTTAATATTGTCGGAATTTATCTGATTAACTACCGGGCCGTAGACCCGGCAGTTATTTT  >  CP009273/1540252‑1540546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A