Notes

Publication:

10.1101/634105

10.1016/j.ymben.2023.01.012

ALE, flask, and isolate counts

ALE replicate Flask count Isolate count
0 1 1
1 1 3
2 1 2
3 1 3
4 1 3
5 1 2
6 1 2
7 1 2
8 1 3
9 9 21

ALE experiment mutation counts

Mutation Type Unique Observed
Single Base Substitutions 1547 2250
Multiple Base Substitutions 1 1
Deletions 54 140
Insertions 57 88
Mobile Element Insertions 15 31
Amplifications 0 0
Gene Conversions 0 0
Inversions 0 0
Total 1674 2510

Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Unassigned Missing Coverage Evidence Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4 2 422.4 11956043 98.9% 11824526 164.0
A1 F50 I1 R1 157 8 93.7 2947622 98.1% 2891617 150.3
A1 F50 I2 R1 121 4 82.0 2593086 97.7% 2533445 150.3
A1 F50 I3 R1 143 4 109.6 3434427 98.5% 3382910 150.4
A2 F50 I1 R1 170 4 39.5 1266514 99.2% 1256381 150.0
A2 F50 I2 R1 173 5 150.8 4954114 96.5% 4780720 150.2
A3 F50 I1 R1 144 3 141.8 4677362 93.6% 4378010 150.0
A3 F50 I2 R1 193 5 39.9 1341687 91.8% 1231668 149.6
A3 F50 I3 R1 160 4 117.2 4331776 85.4% 3699336 149.7
A4 F50 I1 R1 10 3 123.0 3987717 97.5% 3888024 150.3
A4 F50 I2 R1 104 4 44.1 1376607 98.9% 1361464 150.2
A4 F50 I3 R1 105 3 107.9 3491495 95.1% 3320411 150.2
A5 F50 I1 R1 141 3 76.7 2402765 98.4% 2364320 150.1
A5 F50 I2 R1 139 4 64.5 2139856 92.7% 1983646 149.9
A6 F50 I1 R1 11 6 62.7 2040825 94.6% 1930620 149.9
A6 F50 I2 R1 8 10 37.1 8216431 13.3% 1092785 146.7
A7 F50 I1 R1 121 4 119.5 3777739 97.6% 3687073 150.2
A7 F50 I2 R1 209 7 44.2 1360995 98.7% 1343302 150.3
A8 F50 I1 R1 163 57 55.0 1534755 98.6% 1513268 150.0
A8 F50 I2 R1 150 5 68.2 2132745 98.7% 2105019 150.2
A8 F50 I3 R1 168 4 141.0 4346284 99.6% 4328898 150.4

Mutation Needle Plot

Mutation Targets

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