breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,791 G→A intergenic (+160/‑32) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 226,010 (A)6→5 coding (357/804 nt) dkgB → 2,5‑didehydrogluconate reductase DkgB
RA 859,587 G→A A164T (GCA→ACA)  fsa → fructose‑6‑phosphate aldolase
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,631,337 G→A W112* (TGG→TGA pitA → inorganic phosphate transporter PitA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357574 594 3 [0] [2] 4 [lacA] [lacA]
* * ÷ NZ_CP009273 671830 679000 7171 26 [0] [2] 3 [ybeQ]–[rihA] [ybeQ],ybeR,djlB,ybeT,ybeU,djlC,hscC,[rihA]
* * ÷ NZ_CP009273 679028 679217 190 3 [2] [2] 4 rihA pyrimidine‑specific ribonucleoside hydrolase RihA
* * ÷ NZ_CP009273 679276 680302 1027 4 [2] [2] 4 [rihA]–[gltL] [rihA],[gltL]
* * ÷ NZ_CP009273 680351 684425–683302 2952–4075 3 [2] [2] 4 [gltL]–[BW25113_RS03405] [gltL],gltK,gltJ,gltI,[BW25113_RS03405]
* * ÷ NZ_CP009273 903011 903042 32 3 [1] [2] 3 ybjT SDR family oxidoreductase
* * ÷ NZ_CP009273 1428003–1428248 1428279–1428255 8–277 3 [2] [2] 3 [ydfK] [ydfK]
* * ÷ NZ_CP009273 1783647 1783663 17 3 [2] [2] 5 aroH 3‑deoxy‑7‑phosphoheptulonate synthase AroH
* * ÷ NZ_CP009273 1973023 1973033 11 4 [1] [2] 3 flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 269666 =NA (NA)26 (0.900) 16/164 0.5 100% intergenic (+2/+146) ykfC/BW25113_RS01325 reverse transcriptase domain‑containing protein/IS5‑like element IS5 family transposase
?NZ_CP009273 = 671829 0 (0.000)coding (175/978 nt) ybeQ SEL1‑like repeat protein
* ? NZ_CP009273 360104 =NA (NA)28 (0.960) 19/164 0.3 NA intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 NA (NA)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1089701 =NA (NA)16 (0.550) 14/164 0.6 84.2% intergenic (+11/+30) dgcT/BW25113_RS05345 GGDEF domain‑containing protein/IS3‑like element IS3 family transposase
?NZ_CP009273 1417789 = 3 (0.100)coding (68/120 nt) BW25113_RS26130 hypothetical protein
* ? NZ_CP009273 1203246 =35 (1.210)5 (0.210) 5/132 1.5 21.2% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 9 (0.380)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)20 (0.700) 12/162 0.7 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 14177937 (0.240)23 (0.820) 20/158 0.2 77.3% coding (64/120 nt) BW25113_RS26130 hypothetical protein
?NZ_CP009273 2163654 = NA (NA)intergenic (‑4/‑63) BW25113_RS10890/BW25113_RS10900 DeoR/GlpR family DNA‑binding transcription regulator/IS3‑like element IS3 family transposase
* ? NZ_CP009273 = 19727510 (0.000)31 (1.070) 18/164 0.3 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 4532499 =0 (0.000)17 (0.660) 13/146 0.5 69.4% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 15 (0.580)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 45325070 (0.000)10 (0.390) 8/146 1.1 57.1% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 15 (0.580)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA