breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 74 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,223,0130CA53.5% ‑5.6 / 46.5 43L428F (TTG→TTTPP_4603 CDS (transposase)
*AE015451_phaM1699,7220AG52.2% 202.2 / inf 931intergenic (+115/‑129)PP_t06 tRNA (tRNA‑Ala)/PP_23SD rRNA (23S ribosomal RNA)–/–
*AE015451_phaM11,327,1210CT49.8% 926.3 / inf 1598intergenic (+97/‑196)PP_16SE rRNA (16S ribosomal RNA)/PP_23SE rRNA (23S ribosomal RNA)–/–
*AE015451_phaM14,348,7050AG48.2% 269.4 / inf 1483intergenic (‑70/+404)PP_3820 CDS (Group II intron‑encoding maturase)/galU CDS (UTP‑glucose‑1‑phosphate uridylyltransferase)–/–
*AE015451_phaM14,675,3600TG42.9% 189.5 / 59.7 190intergenic (+59/+45)PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)–/–
*AE015451_phaM1195,6340CT37.4% 1436.2 / inf 1176T380T (ACC→ACTPP_0168 CDS (putative surface adhesion protein)
*AE015451_phaM15,737,7560AG34.4% 261.2 / 89.7 224intergenic (+45/+26)hutF CDS (probable formiminoglutamate deiminase)/PP_5037 CDS (lipocalin family lipoprotein)–/–
*AE015451_phaM1196,8790GA33.9% 197.1 / 145.1 168V795V (GTG→GTAPP_0168 CDS (putative surface adhesion protein)
*AE015451_phaM14,889,6440GC33.5% 369.4 / 111.2 215intergenic (+90/‑63)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM14,889,6230AG33.3% 183.8 / 26.9 130intergenic (+69/‑84)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM14,344,3080GA30.7% 124.0 / 17.1 111intergenic (+39/‑117)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM12,178,5680GA30.3% 2359.9 / inf 1282T268T (ACG→ACAPP_1931 CDS (conserved protein of unknown function)
*AE015451_phaM11,210,8490AC29.9% 1055.6 / 106.6 556*540G (TGA→GGA) PP_1060 CDS (putative Glutamate synthase_ large subunit)
*AE015451_phaM14,344,3120GA28.6% 95.3 / 18.9 105intergenic (+43/‑113)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM11,210,8300GT27.9% 1280.1 / 233.7 595intergenic (+20/+17)ytnA CDS (Uncharacterized amino acid permease YtnA)/PP_1060 CDS (putative Glutamate synthase_ large subunit)–/–
*AE015451_phaM15,537,0650TG27.6% 324.9 / 15.2 197intergenic (+42/+61)nadE CDS (NH(3)‑dependent NAD(+) synthetase)/PP_4870 CDS (Azurin)–/–
*AE015451_phaM11,210,8230CG27.4% 1332.6 / 209.4 581intergenic (+13/+24)ytnA CDS (Uncharacterized amino acid permease YtnA)/PP_1060 CDS (putative Glutamate synthase_ large subunit)–/–
*AE015451_phaM14,675,3340AG27.2% 241.6 / 12.6 150intergenic (+33/+71)PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)–/–
*AE015451_phaM15,671,6710CT27.1% 519.8 / 33.4 315intergenic (+90/‑80)PP_4978 CDS (conserved protein of unknown function)/PP_4979 CDS (putative Periplasmic binding protein)–/–
*AE015451_phaM15,199,6570CT26.6% 82.0 / 29.9 127intergenic (+32/+41)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–

Marginal new junction evidence (lowest skew 10 of 206 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 4808174 =46 (0.060)317 (0.510) 47/200 4.3 62.6% intergenic (‑283/‑272) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986211 345 (0.560)intergenic (+229/‑317) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 1780066283 (0.340)158 (0.200) 62/256 4.4 42.7% intergenic (+25/+81) PP_1587 CDS (NhaP‑type Na+_H+ and K+_H+ antiporters)/dapC CDS (N‑succinyl‑L_L‑diaminopimelate aminotransferase alternative) –/–
?AE015451_phaM1 1780129 = 156 (0.200)intergenic (+88/+18) PP_1587 CDS (NhaP‑type Na+_H+ and K+_H+ antiporters)/dapC CDS (N‑succinyl‑L_L‑diaminopimelate aminotransferase alternative) –/–
* ? AE015451_phaM1 = 555560824 (0.030)250 (0.310)
+GCC
55/264 5.9 91.4% intergenic (‑84/+87) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
?AE015451_phaM1 5555609 = 24 (0.030)intergenic (‑85/+86) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
* ? pS4413_qbsFH 1 =0 (0.000)16271 (0.910) 189/270 7.1 100% intergenic (–/‑1952) –/aadA CDS –/–
?pS4413_qbsFH = 5211 0 (0.000)intergenic (‑521/–) rep CDS/– –/–
* ? pS4413_qbsFH = 8137 (0.000)12831 (0.740)
+GCCGC
158/248 10.2 99.7% intergenic (–/‑1872) –/aadA CDS –/–
?pS4413_qbsFH 82 = 37 (0.000)intergenic (–/‑1871) –/aadA CDS –/–
* ? AE015451_phaM1 = 56814152 (0.000)167 (0.210)
+CGGG
33/262 10.9 98.9% intergenic (‑133/+71) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
?AE015451_phaM1 5681416 = 2 (0.000)intergenic (‑134/+70) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
* ? AE015451_phaM1 = 652782624 (0.750)129 (0.180) 27/230 11.1 32.7% coding (1961/1962 nt) ctpL CDS (Methyl‑accepting chemotaxis protein CtpL)
?AE015451_phaM1 = 4808159 0 (0.000)intergenic (‑268/‑287) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
* ? AE015451_phaM1 2478409 =897 (1.070)47 (0.070) 23/218 11.8 6.1% coding (106/444 nt) PP_2172 CDS (conserved exported protein of unknown function)
?AE015451_phaM1 = 2478405 729 (1.080)coding (102/444 nt) PP_2172 CDS (conserved exported protein of unknown function)
* ? AE015451_phaM1 = 2472115242 (0.290)48 (0.060) 28/256 12.2 12.1% intergenic (+19/+130) PP_2166 CDS (Anti‑anti‑sigma factor)/PP_2167 CDS (putative PhoD‑like phosphatase) –/–
?AE015451_phaM1 2472313 = 465 (0.590)coding (1783/1851 nt) PP_2167 CDS (putative PhoD‑like phosphatase)
* ? AE015451_phaM1 = 480817925 (0.030)113 (0.160) 22/230 12.9 35.4% intergenic (‑288/‑267) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986225 392 (0.550)intergenic (+243/‑303) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–