breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 51 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349433,285,6470CA42.9% 211.7 / inf 333V252L (GTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,7130GA37.7% 196.8 / 198.2 168L230L (CTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5800AG37.0% 303.1 / inf 284I274T (ATA→ACA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5750TG34.6% 360.5 / 279.4 286N276H (AAT→CAT) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5670CT33.6% 370.6 / 302.4 292L278L (CTG→CTAEO946_RS16995IS3 family transposase
*NZ_CP0349431,730,0960GC32.2% 178.6 / 36.6 122E173Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349432,991,2360CG31.7% 288.3 / 45.8 169intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,991,2520CA29.9% 283.1 / 31.5 167intergenic (+53/‑154)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP034943634,0540CG28.8% 263.8 / 38.2 143E573Q (GAA→CAA) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349432,991,2270TG27.8% 268.7 / 26.4 170intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP034943177,0530CA27.7% 271.8 / 24.0 155intergenic (+226/‑46)EO946_RS01020/EO946_RS01025alcohol dehydrogenase/SulP family inorganic anion transporter
*NZ_CP0349433,446,9700GT27.0% 336.6 / 59.0 205Q206K (CAA→AAA) degUtwo‑component system response regulator DegU
*NZ_CP0349432,103,7650CA26.7% 177.6 / 17.2 107V7L (GTA→TTA) azoJFMN‑dependent NADH‑azoreductase AzoJ
*NZ_CP0349431,966,2580GC26.6% 199.0 / 21.4 116intergenic (+1/‑452)iseA/EO946_RS09770DL‑endopeptidase inhibitor IseA/hypothetical protein
*NZ_CP0349431,730,0750GC26.3% 240.1 / 19.0 126E166Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349431,111,6490CA26.1% 295.1 / 21.1 149P582T (CCG→ACG) addBhelicase‑exonuclease AddAB subunit AddB
*NZ_CP034943549,1130GC25.4% 413.9 / 29.9 196R42T (AGA→ACA) EO946_RS02865PLP‑dependent aminotransferase family protein
*NZ_CP0349432,991,2450GT25.1% 284.4 / 44.7 171intergenic (+46/‑161)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP034943549,0800GT25.1% 421.8 / 25.9 212R31L (CGT→CTT) EO946_RS02865PLP‑dependent aminotransferase family protein
*NZ_CP0349431,366,1090GT24.5% 307.5 / 14.2 170D203Y (GAT→TAT) EO946_RS07135PepSY domain‑containing protein

Marginal new junction evidence (lowest skew 10 of 20 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 607483213 (0.950)8 (0.040) 6/242 8.6 3.8% coding (326/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607727 = 215 (1.070)coding (570/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607695 =158 (0.710)9 (0.040)
+AGTAGCCCCT
6/250 8.8 4.4% coding (538/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 264 (1.180)coding (689/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607713 =224 (1.000)11 (0.050) 6/260 9.1 4.6% coding (556/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607721 238 (1.100)coding (564/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 767776 =234 (1.050)5 (0.020) 5/244 9.2 2.2% coding (189/645 nt) EO946_RS03930 hypothetical protein
?NZ_CP034943 = 767772 224 (1.110)coding (193/645 nt) EO946_RS03930 hypothetical protein
* ? NZ_CP034943 = 165756262 (1.170)4 (0.020) 4/224 9.3 1.5% noncoding (65/73 nt) EO946_RS00965 tRNA‑Thr
?NZ_CP034943 166185 = 300 (1.620)noncoding (66/76 nt) EO946_RS00985 tRNA‑Ala
* ? NZ_CP034943 = 607225303 (1.350)6 (0.030)
+AGGGGCTACTGGGGACGT
4/234 9.5 2.8% coding (68/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607703 = 180 (0.800)coding (546/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607355241 (1.080)8 (0.040)
+GCGACAGGAGCTACC
4/240 9.7 4.4% coding (198/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607587 = 153 (0.680)coding (430/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607745254 (1.130)4 (0.020)
+21 bp
3/228 10.0 1.7% coding (588/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607839 = 293 (1.310)coding (682/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607673149 (0.670)4 (0.020)
+21 bp
3/228 10.0 2.1% coding (516/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607839 = 293 (1.310)coding (682/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607561199 (0.890)5 (0.030)
+ACCAACCGGAGCGACG
3/238 10.3 2.3% coding (404/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607839 = 292 (1.300)coding (682/1125 nt) EO946_RS03185 collagen‑like protein