breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 197 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606720,4660GC62.5% ‑4.5 / 21.0 24A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6062,515,9090AC48.6% 69.1 / 47.0 72V260G (GTG→GGG) ypfIpredicted hydrolase
*REL6061,747,9030GT47.4% 285.3 / inf 462A8S (GCA→TCA) ydiLhypothetical protein
*REL6064,180,2490TG46.0% 107.1 / 38.7 87G24G (GGT→GGGyjaHhypothetical protein
*REL6061,854,4010AC42.9% 99.9 / 39.8 84K88N (AAA→AACyeaOhypothetical protein
*REL6064330TG41.8% 133.3 / 50.6 104V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6062,437,4060GT40.5% 41.8 / 34.7 42D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL6063,774,9140CG38.9% 274.5 / 139.1 167A20P (GCC→CCC) yicJpredicted transporter
*REL6062,356,0140TG36.7% 388.7 / 38.6 199T58P (ACC→CCC) yfbUhypothetical protein
*REL6062,044,3570AC36.1% 359.9 / 93.5 196V284G (GTG→GGG) rfbDdTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase
*REL6062,376,3480TG36.1% 192.3 / 38.4 122T63P (ACC→CCC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*REL6062,144,2950AC36.0% 374.6 / 82.8 198G357G (GGT→GGGmrpantiporter inner membrane protein
*REL6061,485,4500AG35.5% 331.6 / 120.3 187G193G (GGA→GGGydcVpredicted spermidine/putrescine transporter subunit
*REL6061,853,0670AC35.5% 227.8 / 72.0 141T56P (ACC→CCC) yeaNpredicted transporter
*REL606390,9570AC35.2% 407.3 / 66.6 196H7P (CAC→CCC) malZmaltodextrin glucosidase
*REL6063,099,0910AC34.9% 211.5 / 20.9 103V352G (GTG→GGG) parCDNA topoisomerase IV subunit A
*REL6062,007,0280CA34.6% 48.3 / 18.5 26intergenic (‑152/+503)insC/ECB_01906insertion sequence 2 OrfA protein; b1997/conserved hypothetical protein
*REL6063,765,1500AC34.3% 272.2 / 34.4 134K528N (AAA→AACrecGATP‑dependent DNA helicase
*REL6063,096,3540AC34.2% 244.9 / 61.6 146V163G (GTG→GGG) sufIrepressor protein for FtsI
*REL6064,392,5560CT34.2% 190.9 / 88.8 146L43L (CTA→TTA) aidBisovaleryl CoA dehydrogenase

Marginal new junction evidence (lowest skew 10 of 16 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3026635 =238 (1.020)5 (0.030) 4/128 17.0 2.6% coding (168/1377 nt) ECB_02826 hypothetical protein
?REL606 3026802 = 175 (0.880)coding (1/1377 nt) ECB_02826 hypothetical protein
* ? REL606 = 2680757343 (1.470)4 (0.020) 4/138 17.7 1.4% coding (283/897 nt) ECB_02511 ParB family protein
?REL606 = 2681225 263 (1.220)coding (751/897 nt) ECB_02511 ParB family protein
* ? REL606 3412449 =228 (0.980)3 (0.010) 3/130 18.0 1.5% coding (55/870 nt) prkB predicted phosphoribulokinase
?REL606 = 3412445 186 (0.920)coding (51/870 nt) prkB predicted phosphoribulokinase
* ? REL606 = 3022906168 (0.720)3 (0.010) 3/134 18.2 1.9% intergenic (‑222/+352) ECB_02821/ECB_02822 hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
?REL606 = 3023096 168 (0.810)intergenic (‑412/+162) ECB_02821/ECB_02822 hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase
* ? REL606 2019640 =324 (1.390)3 (0.010) 3/134 18.2 1.1% intergenic (‑69/‑358) yeeZ/hisL predicted epimerase, with NAD(P)‑binding Rossmann‑fold domain/his operon leader peptide
?REL606 2019892 = 259 (1.240)intergenic (‑321/‑106) yeeZ/hisL predicted epimerase, with NAD(P)‑binding Rossmann‑fold domain/his operon leader peptide
* ? REL606 = 3739251339 (1.450)4 (0.020) 3/136 18.3 1.5% coding (715/1026 nt) waaW UDP‑galactose:(Galactosyl) LPS alpha1,2‑galactosyltransferase
?REL606 = 3739692 212 (1.000)coding (274/1026 nt) waaW UDP‑galactose:(Galactosyl) LPS alpha1,2‑galactosyltransferase
* ? REL606 = 1347618265 (1.130)3 (0.010) 3/136 18.3 1.2% coding (859/1128 nt) yciW predicted oxidoreductase
?REL606 = 1347850 264 (1.250)coding (627/1128 nt) yciW predicted oxidoreductase
* ? REL606 = 1128081177 (0.760)186 (0.800) 4/150 18.3 34.6% intergenic (+106/‑67) mdoH/yceK glucosyltransferase MdoH/predicted lipoprotein
?REL606 = 2583282 526 (2.250)coding (741/741 nt) yfhQ predicted methyltransferase
* ? REL606 791599 =258 (1.100)3 (0.010) 3/136 18.3 1.3% coding (145/552 nt) ECB_00732 conserved hypothetical protein
?REL606 791823 = 215 (1.020)coding (369/552 nt) ECB_00732 conserved hypothetical protein
* ? REL606 2004536 =250 (1.070)4 (0.020) 3/138 18.4 1.5% intergenic (‑772/‑900) cobU/yoeA adenosylcobinamide kinase/adenosylcobinamide‑phosphate guanylyltransferase/b1995(b4582); putative hemine receptor
?REL606 2005028 = 295 (1.370)intergenic (‑1264/‑408) cobU/yoeA adenosylcobinamide kinase/adenosylcobinamide‑phosphate guanylyltransferase/b1995(b4582); putative hemine receptor