breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 197 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 720,466 | 0 | G | C | 62.5% | ‑4.5 / 21.0 | 24 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 2,515,909 | 0 | A | C | 48.6% | 69.1 / 47.0 | 72 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 1,747,903 | 0 | G | T | 47.4% | 285.3 / inf | 462 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 4,180,249 | 0 | T | G | 46.0% | 107.1 / 38.7 | 87 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 1,854,401 | 0 | A | C | 42.9% | 99.9 / 39.8 | 84 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 433 | 0 | T | G | 41.8% | 133.3 / 50.6 | 104 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 2,437,406 | 0 | G | T | 40.5% | 41.8 / 34.7 | 42 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
* | REL606 | 3,774,914 | 0 | C | G | 38.9% | 274.5 / 139.1 | 167 | A20P (GCC→CCC) | yicJ | predicted transporter |
* | REL606 | 2,356,014 | 0 | T | G | 36.7% | 388.7 / 38.6 | 199 | T58P (ACC→CCC) | yfbU | hypothetical protein |
* | REL606 | 2,044,357 | 0 | A | C | 36.1% | 359.9 / 93.5 | 196 | V284G (GTG→GGG) | rfbD | dTDP‑4‑dehydrorhamnose reductase subunit, NAD(P)‑binding, of dTDP‑L‑rhamnose synthase |
* | REL606 | 2,376,348 | 0 | T | G | 36.1% | 192.3 / 38.4 | 122 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
* | REL606 | 2,144,295 | 0 | A | C | 36.0% | 374.6 / 82.8 | 198 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
* | REL606 | 1,485,450 | 0 | A | G | 35.5% | 331.6 / 120.3 | 187 | G193G (GGA→GGG) | ydcV | predicted spermidine/putrescine transporter subunit |
* | REL606 | 1,853,067 | 0 | A | C | 35.5% | 227.8 / 72.0 | 141 | T56P (ACC→CCC) | yeaN | predicted transporter |
* | REL606 | 390,957 | 0 | A | C | 35.2% | 407.3 / 66.6 | 196 | H7P (CAC→CCC) | malZ | maltodextrin glucosidase |
* | REL606 | 3,099,091 | 0 | A | C | 34.9% | 211.5 / 20.9 | 103 | V352G (GTG→GGG) | parC | DNA topoisomerase IV subunit A |
* | REL606 | 2,007,028 | 0 | C | A | 34.6% | 48.3 / 18.5 | 26 | intergenic (‑152/+503) | insC/ECB_01906 | insertion sequence 2 OrfA protein; b1997/conserved hypothetical protein |
* | REL606 | 3,765,150 | 0 | A | C | 34.3% | 272.2 / 34.4 | 134 | K528N (AAA→AAC) | recG | ATP‑dependent DNA helicase |
* | REL606 | 3,096,354 | 0 | A | C | 34.2% | 244.9 / 61.6 | 146 | V163G (GTG→GGG) | sufI | repressor protein for FtsI |
* | REL606 | 4,392,556 | 0 | C | T | 34.2% | 190.9 / 88.8 | 146 | L43L (CTA→TTA) | aidB | isovaleryl CoA dehydrogenase |
Marginal new junction evidence (lowest skew 10 of 16 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 3026635 = | 238 (1.020) | 5 (0.030) | 4/128 | 17.0 | 2.6% | coding (168/1377 nt) | ECB_02826 | hypothetical protein |
? | REL606 | 3026802 = | 175 (0.880) | coding (1/1377 nt) | ECB_02826 | hypothetical protein | |||||
* | ? | REL606 | = 2680757 | 343 (1.470) | 4 (0.020) | 4/138 | 17.7 | 1.4% | coding (283/897 nt) | ECB_02511 | ParB family protein |
? | REL606 | = 2681225 | 263 (1.220) | coding (751/897 nt) | ECB_02511 | ParB family protein | |||||
* | ? | REL606 | 3412449 = | 228 (0.980) | 3 (0.010) | 3/130 | 18.0 | 1.5% | coding (55/870 nt) | prkB | predicted phosphoribulokinase |
? | REL606 | = 3412445 | 186 (0.920) | coding (51/870 nt) | prkB | predicted phosphoribulokinase | |||||
* | ? | REL606 | = 3022906 | 168 (0.720) | 3 (0.010) | 3/134 | 18.2 | 1.9% | intergenic (‑222/+352) | ECB_02821/ECB_02822 | hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase |
? | REL606 | = 3023096 | 168 (0.810) | intergenic (‑412/+162) | ECB_02821/ECB_02822 | hypothetical protein/bifunctional Ribulose 5‑phosphate reductase/CDP‑ribitol pyrophosphorylase | |||||
* | ? | REL606 | 2019640 = | 324 (1.390) | 3 (0.010) | 3/134 | 18.2 | 1.1% | intergenic (‑69/‑358) | yeeZ/hisL | predicted epimerase, with NAD(P)‑binding Rossmann‑fold domain/his operon leader peptide |
? | REL606 | 2019892 = | 259 (1.240) | intergenic (‑321/‑106) | yeeZ/hisL | predicted epimerase, with NAD(P)‑binding Rossmann‑fold domain/his operon leader peptide | |||||
* | ? | REL606 | = 3739251 | 339 (1.450) | 4 (0.020) | 3/136 | 18.3 | 1.5% | coding (715/1026 nt) | waaW | UDP‑galactose:(Galactosyl) LPS alpha1,2‑galactosyltransferase |
? | REL606 | = 3739692 | 212 (1.000) | coding (274/1026 nt) | waaW | UDP‑galactose:(Galactosyl) LPS alpha1,2‑galactosyltransferase | |||||
* | ? | REL606 | = 1347618 | 265 (1.130) | 3 (0.010) | 3/136 | 18.3 | 1.2% | coding (859/1128 nt) | yciW | predicted oxidoreductase |
? | REL606 | = 1347850 | 264 (1.250) | coding (627/1128 nt) | yciW | predicted oxidoreductase | |||||
* | ? | REL606 | = 1128081 | 177 (0.760) | 186 (0.800) | 4/150 | 18.3 | 34.6% | intergenic (+106/‑67) | mdoH/yceK | glucosyltransferase MdoH/predicted lipoprotein |
? | REL606 | = 2583282 | 526 (2.250) | coding (741/741 nt) | yfhQ | predicted methyltransferase | |||||
* | ? | REL606 | 791599 = | 258 (1.100) | 3 (0.010) | 3/136 | 18.3 | 1.3% | coding (145/552 nt) | ECB_00732 | conserved hypothetical protein |
? | REL606 | 791823 = | 215 (1.020) | coding (369/552 nt) | ECB_00732 | conserved hypothetical protein | |||||
* | ? | REL606 | 2004536 = | 250 (1.070) | 4 (0.020) | 3/138 | 18.4 | 1.5% | intergenic (‑772/‑900) | cobU/yoeA | adenosylcobinamide kinase/adenosylcobinamide‑phosphate guanylyltransferase/b1995(b4582); putative hemine receptor |
? | REL606 | 2005028 = | 295 (1.370) | intergenic (‑1264/‑408) | cobU/yoeA | adenosylcobinamide kinase/adenosylcobinamide‑phosphate guanylyltransferase/b1995(b4582); putative hemine receptor |