breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 259 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 2,515,909 | 0 | A | C | 53.4% | 51.8 / 109.7 | 74 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 720,464 | 0 | C | G | 51.2% | 5.3 / 67.9 | 41 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 1,854,401 | 0 | A | C | 48.6% | 111.5 / 54.7 | 109 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 2,437,406 | 0 | G | T | 48.4% | 24.6 / 26.1 | 32 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
* | REL606 | 720,466 | 0 | G | C | 48.3% | 2.7 / 22.4 | 29 | A23P (GCC→CCC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 1,096,032 | 0 | C | G | 47.2% | 84.5 / 129.5 | 106 | G148G (GGC→GGG) | putP | proline:sodium symporter |
* | REL606 | 3,783,565 | 0 | A | C | 46.0% | 122.3 / 73.0 | 111 | T744P (ACA→CCA) | ECB_03521 | conserved hypothetical protein |
* | REL606 | 2,735,608 | 0 | G | C | 45.5% | 34.3 / 19.9 | 33 | A409P (GCC→CCC) | ascF | fused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component |
* | REL606 | 2,376,348 | 0 | T | G | 44.0% | 274.3 / 62.3 | 193 | T63P (ACC→CCC) | folC | bifunctional folylpolyglutamate synthase/ dihydrofolate synthase |
* | REL606 | 4,180,249 | 0 | T | G | 42.9% | 223.9 / 48.8 | 133 | G24G (GGT→GGG) | yjaH | hypothetical protein |
* | REL606 | 433 | 0 | T | G | 41.7% | 93.4 / 30.7 | 60 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 1,118,209 | 0 | G | T | 40.8% | 38.0 / 53.8 | 49 | intergenic (‑444/‑311) | csgD/csgB | DNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex |
* | REL606 | 1,853,067 | 0 | A | C | 38.6% | 255.7 / 136.0 | 179 | T56P (ACC→CCC) | yeaN | predicted transporter |
* | REL606 | 3,472,700 | 0 | C | G | 38.5% | 114.8 / 88.0 | 96 | A290G (GCG→GGG) | yhgA | predicted transposase |
* | REL606 | 2,676,636 | 0 | T | G | 37.0% | 428.0 / 96.5 | 238 | V149G (GTG→GGG) | smpB | SsrA‑binding protein |
* | REL606 | 1,485,450 | 0 | A | G | 36.6% | 392.7 / 152.3 | 232 | G193G (GGA→GGG) | ydcV | predicted spermidine/putrescine transporter subunit |
* | REL606 | 925,639 | 0 | T | G | 36.2% | 216.9 / 33.4 | 116 | K121N (AAA→AAC) | ltaE | L‑allo‑threonine aldolase, PLP‑dependent |
* | REL606 | 4,111,429 | 0 | T | G | 36.2% | 162.1 / 22.0 | 94 | G496G (GGT→GGG) | metL | bifunctional aspartate kinase II/homoserine dehydrogenase II |
* | REL606 | 931,314 | 0 | T | C | 35.7% | 154.1 / 35.7 | 112 | E84G (GAG→GGG) | ybjE | predicted transporter |
* | REL606 | 159,654 | 0 | A | C | 35.2% | 562.9 / 25.3 | 261 | G27G (GGT→GGG) | yadN | predicted fimbrial‑like adhesin protein |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | = 379585 | 298 (1.220) | 105 (0.430) | 5/150 | 18.5 | 24.5% | intergenic (+110/+135) | yajF/araJ | fructokinase/predicted transporter |
? | REL606 | = 2583282 | 349 (1.430) | coding (741/741 nt) | yfhQ | predicted methyltransferase | |||||
* | ? | REL606 | 2853309 = | 229 (0.940) | 5 (0.020) | 4/150 | 19.2 | 2.1% | coding (195/2889 nt) | ptr | protease III |
? | REL606 | = 4415035 | 228 (0.940) | coding (173/1413 nt) | cycA | D‑alanine/D‑serine/glycine transporter | |||||
* | ? | REL606 | 2486933 = | 217 (0.890) | 3 (0.010) | 3/138 | 19.3 | 1.4% | coding (681/1404 nt) | eutA | reactivating factor for ethanolamine ammonia lyase |
? | REL606 | 2983303 = | 235 (1.050) | coding (286/1137 nt) | yggW | coproporphyrinogen III oxidase | |||||
* | ? | REL606 | 78526 = | 276 (1.130) | 3 (0.010) | 3/150 | 20.0 | 1.2% | coding (1578/1656 nt) | yabN | DNA‑binding transcriptional regulator |
? | REL606 | = 1373085 | 238 (0.980) | coding (628/843 nt) | ycjP | predicted sugar transporter subunit: membrane component of ABC superfamily |