breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 259 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,515,9090AC53.4% 51.8 / 109.7 74V260G (GTG→GGG) ypfIpredicted hydrolase
*REL606720,4640CG51.2% 5.3 / 67.9 41A22G (GCC→GGC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6061,854,4010AC48.6% 111.5 / 54.7 109K88N (AAA→AACyeaOhypothetical protein
*REL6062,437,4060GT48.4% 24.6 / 26.1 32D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL606720,4660GC48.3% 2.7 / 22.4 29A23P (GCC→CCC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6061,096,0320CG47.2% 84.5 / 129.5 106G148G (GGC→GGGputPproline:sodium symporter
*REL6063,783,5650AC46.0% 122.3 / 73.0 111T744P (ACA→CCA) ECB_03521conserved hypothetical protein
*REL6062,735,6080GC45.5% 34.3 / 19.9 33A409P (GCC→CCC) ascFfused cellobiose/arbutin/salicin‑specific PTS enzymes: IIB component/IC component
*REL6062,376,3480TG44.0% 274.3 / 62.3 193T63P (ACC→CCC) folCbifunctional folylpolyglutamate synthase/ dihydrofolate synthase
*REL6064,180,2490TG42.9% 223.9 / 48.8 133G24G (GGT→GGGyjaHhypothetical protein
*REL6064330TG41.7% 93.4 / 30.7 60V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6061,118,2090GT40.8% 38.0 / 53.8 49intergenic (‑444/‑311)csgD/csgBDNA‑binding transcriptional activator in two‑component regulatory system/curlin nucleator protein, minor subunit in curli complex
*REL6061,853,0670AC38.6% 255.7 / 136.0 179T56P (ACC→CCC) yeaNpredicted transporter
*REL6063,472,7000CG38.5% 114.8 / 88.0 96A290G (GCG→GGG) yhgApredicted transposase
*REL6062,676,6360TG37.0% 428.0 / 96.5 238V149G (GTG→GGG) smpBSsrA‑binding protein
*REL6061,485,4500AG36.6% 392.7 / 152.3 232G193G (GGA→GGGydcVpredicted spermidine/putrescine transporter subunit
*REL606925,6390TG36.2% 216.9 / 33.4 116K121N (AAA→AACltaEL‑allo‑threonine aldolase, PLP‑dependent
*REL6064,111,4290TG36.2% 162.1 / 22.0 94G496G (GGT→GGGmetLbifunctional aspartate kinase II/homoserine dehydrogenase II
*REL606931,3140TC35.7% 154.1 / 35.7 112E84G (GAG→GGG) ybjEpredicted transporter
*REL606159,6540AC35.2% 562.9 / 25.3 261G27G (GGT→GGGyadNpredicted fimbrial‑like adhesin protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 379585298 (1.220)105 (0.430) 5/150 18.5 24.5% intergenic (+110/+135) yajF/araJ fructokinase/predicted transporter
?REL606 = 2583282 349 (1.430)coding (741/741 nt) yfhQ predicted methyltransferase
* ? REL606 2853309 =229 (0.940)5 (0.020) 4/150 19.2 2.1% coding (195/2889 nt) ptr protease III
?REL606 = 4415035 228 (0.940)coding (173/1413 nt) cycA D‑alanine/D‑serine/glycine transporter
* ? REL606 2486933 =217 (0.890)3 (0.010) 3/138 19.3 1.4% coding (681/1404 nt) eutA reactivating factor for ethanolamine ammonia lyase
?REL606 2983303 = 235 (1.050)coding (286/1137 nt) yggW coproporphyrinogen III oxidase
* ? REL606 78526 =276 (1.130)3 (0.010) 3/150 20.0 1.2% coding (1578/1656 nt) yabN DNA‑binding transcriptional regulator
?REL606 = 1373085 238 (0.980)coding (628/843 nt) ycjP predicted sugar transporter subunit: membrane component of ABC superfamily