breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,383,1570TG53.8% 4.7 / 32.7 26G224G (GGT→GGGycjXconserved protein with nucleoside triphosphate hydrolase domain
*REL6064,393,0610TG46.2% 36.3 / 22.7 39V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6062,197,8670TG42.2% 54.4 / 24.4 45A47A (GCA→GCCcirAferric iron‑catecholate outer membrane transporter
*REL6063,492,0820TG41.2% 62.1 / 31.4 51V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6061,688,9900AC34.8% 88.5 / 10.2 46T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL6062,231,8920AC33.3% 91.6 / 10.9 48T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*REL6063,733,7190CG33.3% 72.4 / 19.8 39G63G (GGC→GGGgumDADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding
*REL6061,484,1050AG32.3% 53.0 / 13.0 31G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL6061,882,5260TG31.3% 122.5 / 11.4 67I21S (ATT→AGT) manZmannose‑specific enzyme IID component of PTS
*REL6062,296,3290AC31.2% 96.2 / 17.7 48intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6062,631,7720AC31.2% 98.5 / 12.2 48intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*REL6063,983,7000TG30.6% 62.3 / 15.4 36T397P (ACC→CCC) ECB_03713conserved hypothetical protein
*REL6061,875,4830GC29.4% 221.7 / 97.7 109intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL6063,998,7870AC28.7% 172.1 / 17.6 81T31P (ACC→CCC) ubiBputative ubiquinone biosynthesis protein UbiB
*REL6061,747,9030GT27.6% 210.7 / 62.4 87A8S (GCA→TCA) ydiLhypothetical protein
*REL6064,169,2320AT27.0% 167.0 / 14.2 64intergenic (+48/‑165)rpoC/htrCDNA‑directed RNA polymerase subunit beta'/heat shock protein
*REL6061,416,8380TG26.2% 128.5 / 17.3 65intergenic (+1/‑4)ECB_01334/ECB_01335putative Lysozyme/conserved hypothetical protein
*REL6061,004,1060AC25.8% 151.6 / 15.5 62intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL606154,4970TG21.9% 194.7 / 10.3 73A191A (GCA→GCCyadLpredicted fimbrial‑like adhesin protein
*REL6064,141,0090GA21.3% 159.8 / 12.9 61E28K (GAA→AAA) yijCDNA‑binding transcriptional repressor

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 2039076 =44 (0.750)11 (0.210) 7/136 4.5 16.9% coding (619/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 2039562 = 68 (1.280)coding (133/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 2040022 =53 (0.900)4 (0.080) 4/136 5.7 7.5% coding (107/438 nt) vioB VioB, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2040134 = 51 (0.960)intergenic (‑6/+137) vioB/vioA VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 2039283 =78 (1.330)6 (0.110) 4/142 5.9 7.3% coding (412/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 2039547 = 78 (1.400)coding (148/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 = 203993460 (1.020)3 (0.060) 3/134 6.2 5.9% coding (195/438 nt) vioB VioB, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 = 2040066 43 (0.820)coding (63/438 nt) vioB VioB, involved in dTDP‑N‑acetylviosamine synthesis
* ? REL606 2037842 =57 (0.970)3 (0.060) 3/134 6.2 5.5% coding (289/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 2037944 = 52 (0.990)coding (187/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 2036832 =50 (0.850)8 (0.150) 3/136 6.3 15.5% coding (127/1113 nt) wbbC putative glycosyltransferase WbbC
?REL606 2037060 = 42 (0.790)coding (1071/1176 nt) wzy O antigen polymerase (Wzy)
* ? REL606 3022200 =78 (1.330)6 (0.110) 3/142 6.4 6.8% coding (485/1140 nt) ECB_02821 hypothetical protein
?REL606 3022657 = 91 (1.640)coding (28/1140 nt) ECB_02821 hypothetical protein
* ? REL606 2890387 =69 (1.170)4 (0.070) 3/146 6.5 5.6% coding (1155/1779 nt) ygeV predicted DNA‑binding transcriptional regulator
?REL606 2890389 = 67 (1.170)coding (1153/1779 nt) ygeV predicted DNA‑binding transcriptional regulator