breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 1,383,157 | 0 | T | G | 53.8% | 4.7 / 32.7 | 26 | G224G (GGT→GGG) | ycjX | conserved protein with nucleoside triphosphate hydrolase domain |
* | REL606 | 4,393,061 | 0 | T | G | 46.2% | 36.3 / 22.7 | 39 | V211G (GTG→GGG) | aidB | isovaleryl CoA dehydrogenase |
* | REL606 | 2,197,867 | 0 | T | G | 42.2% | 54.4 / 24.4 | 45 | A47A (GCA→GCC) | cirA | ferric iron‑catecholate outer membrane transporter |
* | REL606 | 3,492,082 | 0 | T | G | 41.2% | 62.1 / 31.4 | 51 | V490G (GTG→GGG) | glpD | sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding |
* | REL606 | 1,688,990 | 0 | A | C | 34.8% | 88.5 / 10.2 | 46 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) |
* | REL606 | 2,231,892 | 0 | A | C | 33.3% | 91.6 / 10.9 | 48 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | REL606 | 3,733,719 | 0 | C | G | 33.3% | 72.4 / 19.8 | 39 | G63G (GGC→GGG) | gumD | ADP‑L‑glycero‑D‑mannoheptose‑6‑epimerase, NAD(P)‑binding |
* | REL606 | 1,484,105 | 0 | A | G | 32.3% | 53.0 / 13.0 | 31 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | REL606 | 1,882,526 | 0 | T | G | 31.3% | 122.5 / 11.4 | 67 | I21S (ATT→AGT) | manZ | mannose‑specific enzyme IID component of PTS |
* | REL606 | 2,296,329 | 0 | A | C | 31.2% | 96.2 / 17.7 | 48 | intergenic (‑149/‑66) | inaA/yfaH | hypothetical protein/hypothetical protein |
* | REL606 | 2,631,772 | 0 | A | C | 31.2% | 98.5 / 12.2 | 48 | intergenic (‑267/‑141) | rpoE/nadB | RNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase |
* | REL606 | 3,983,700 | 0 | T | G | 30.6% | 62.3 / 15.4 | 36 | T397P (ACC→CCC) | ECB_03713 | conserved hypothetical protein |
* | REL606 | 1,875,483 | 0 | G | C | 29.4% | 221.7 / 97.7 | 109 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 3,998,787 | 0 | A | C | 28.7% | 172.1 / 17.6 | 81 | T31P (ACC→CCC) | ubiB | putative ubiquinone biosynthesis protein UbiB |
* | REL606 | 1,747,903 | 0 | G | T | 27.6% | 210.7 / 62.4 | 87 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 4,169,232 | 0 | A | T | 27.0% | 167.0 / 14.2 | 64 | intergenic (+48/‑165) | rpoC/htrC | DNA‑directed RNA polymerase subunit beta'/heat shock protein |
* | REL606 | 1,416,838 | 0 | T | G | 26.2% | 128.5 / 17.3 | 65 | intergenic (+1/‑4) | ECB_01334/ECB_01335 | putative Lysozyme/conserved hypothetical protein |
* | REL606 | 1,004,106 | 0 | A | C | 25.8% | 151.6 / 15.5 | 62 | intergenic (‑32/+570) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
* | REL606 | 154,497 | 0 | T | G | 21.9% | 194.7 / 10.3 | 73 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
* | REL606 | 4,141,009 | 0 | G | A | 21.3% | 159.8 / 12.9 | 61 | E28K (GAA→AAA) | yijC | DNA‑binding transcriptional repressor |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 2039076 = | 44 (0.750) | 11 (0.210) | 7/136 | 4.5 | 16.9% | coding (619/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | 2039562 = | 68 (1.280) | coding (133/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | 2040022 = | 53 (0.900) | 4 (0.080) | 4/136 | 5.7 | 7.5% | coding (107/438 nt) | vioB | VioB, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | 2040134 = | 51 (0.960) | intergenic (‑6/+137) | vioB/vioA | VioB, involved in dTDP‑N‑acetylviosamine synthesis/VioA, involved in dTDP‑N‑acetylviosamine synthesis | |||||
* | ? | REL606 | 2039283 = | 78 (1.330) | 6 (0.110) | 4/142 | 5.9 | 7.3% | coding (412/747 nt) | wbbA | putative glycosyltransferase WbbA |
? | REL606 | 2039547 = | 78 (1.400) | coding (148/747 nt) | wbbA | putative glycosyltransferase WbbA | |||||
* | ? | REL606 | = 2039934 | 60 (1.020) | 3 (0.060) | 3/134 | 6.2 | 5.9% | coding (195/438 nt) | vioB | VioB, involved in dTDP‑N‑acetylviosamine synthesis |
? | REL606 | = 2040066 | 43 (0.820) | coding (63/438 nt) | vioB | VioB, involved in dTDP‑N‑acetylviosamine synthesis | |||||
* | ? | REL606 | 2037842 = | 57 (0.970) | 3 (0.060) | 3/134 | 6.2 | 5.5% | coding (289/1176 nt) | wzy | O antigen polymerase (Wzy) |
? | REL606 | 2037944 = | 52 (0.990) | coding (187/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | 2036832 = | 50 (0.850) | 8 (0.150) | 3/136 | 6.3 | 15.5% | coding (127/1113 nt) | wbbC | putative glycosyltransferase WbbC |
? | REL606 | 2037060 = | 42 (0.790) | coding (1071/1176 nt) | wzy | O antigen polymerase (Wzy) | |||||
* | ? | REL606 | 3022200 = | 78 (1.330) | 6 (0.110) | 3/142 | 6.4 | 6.8% | coding (485/1140 nt) | ECB_02821 | hypothetical protein |
? | REL606 | 3022657 = | 91 (1.640) | coding (28/1140 nt) | ECB_02821 | hypothetical protein | |||||
* | ? | REL606 | 2890387 = | 69 (1.170) | 4 (0.070) | 3/146 | 6.5 | 5.6% | coding (1155/1779 nt) | ygeV | predicted DNA‑binding transcriptional regulator |
? | REL606 | 2890389 = | 67 (1.170) | coding (1153/1779 nt) | ygeV | predicted DNA‑binding transcriptional regulator |