breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
JC | 474,106 | Δ15 bp | coding (1186‑1200/1677 nt) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
JC JC | 1,305,487 | IS150 (–) +3 bp | coding (751‑753/1461 nt) | cls ← | cardiolipin synthetase |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
RA | 1,645,442 | G→A | S106S (AGC→AGT) | mlc ← | DNA‑binding transcriptional repressor |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,928,260 | T→A | I15N (ATC→AAC) | rho → | transcription termination factor Rho |
RA | 4,202,404 | T→G | Q52H (CAA→CAC) | iclR ← | DNA‑binding transcriptional repressor |
RA | 4,404,030 | T→A | F13I (TTC→ATC) | rpsR → | 30S ribosomal protein S18 |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 546989–547699 | 559313 | 11615–12325 | 11 [10] | [9] 11 | [insB‑6]–[appY] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,[appY] |
* | * | ÷ | REL606 | 560147 | 568938–568921 | 8775–8792 | 11 [10] | [1] 77 | [ompT]–[yhhI] | [ompT],envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,[yhhI] |
* | * | ÷ | REL606 | 1500330–1503173 | 1503691–1503181 | 9–3362 | 11 [9] | [9] 11 | rhsE | rhsE element core protein RshE |
* | * | ÷ | REL606 | 1610343 | 1613912 | 3570 | 11 [10] | [10] 11 | ECB_01516–[ECB_01522] | ECB_01516,cspI,ydfP,ydfQ,ydfR,essQ,[ECB_01522] |
* | * | ÷ | REL606 | 1614759 | 1616661–1615469 | 711–1903 | 11 [10] | [9] 11 | [ECB_01523]–[insF‑4] | [ECB_01523],insE‑4,[insF‑4] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 568939 = | 1 (0.020) | 76 (1.640) | 50/150 | 0.0 | 98.7% | coding (998/1137 nt) | yhhI | predicted transposase |
? | REL606 | = 619884 | NA (NA) | noncoding (1/768 nt) | IS1 | repeat region |