breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
JC 474,106 Δ15 bp coding (1186‑1200/1677 nt) ybaL ← predicted transporter with NAD(P)‑binding Rossmann‑fold domain
JC JC 1,305,487 IS150 (–) +3 bp coding (751‑753/1461 nt) cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,645,442 G→A S106S (AGC→AGT mlc ← DNA‑binding transcriptional repressor
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,928,260 T→A I15N (ATC→AAC)  rho → transcription termination factor Rho
RA 4,202,404 T→G Q52H (CAA→CAC iclR ← DNA‑binding transcriptional repressor
RA 4,404,030 T→A F13I (TTC→ATC)  rpsR → 30S ribosomal protein S18
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 546989–547699 559313 11615–12325 11 [10] [9] 11 [insB‑6]–[appY] [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,[appY]
* * ÷ REL606 560147 568938–568921 8775–8792 11 [10] [1] 77 [ompT]–[yhhI] [ompT],envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,[yhhI]
* * ÷ REL606 1500330–1503173 1503691–1503181 9–3362 11 [9] [9] 11 rhsE rhsE element core protein RshE
* * ÷ REL606 1610343 1613912 3570 11 [10] [10] 11 ECB_01516–[ECB_01522] ECB_01516,cspI,ydfP,ydfQ,ydfR,essQ,[ECB_01522]
* * ÷ REL606 1614759 1616661–1615469 711–1903 11 [10] [9] 11 [ECB_01523]–[insF‑4] [ECB_01523],insE‑4,[insF‑4]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 568939 =1 (0.020)76 (1.640) 50/150 0.0 98.7% coding (998/1137 nt) yhhI predicted transposase
?REL606 = 619884 NA (NA)noncoding (1/768 nt) IS1 repeat region