breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 636,546 C→A F446L (TTC→TTA ahpF → alkyl hydroperoxide reductase, F52a subunit, FAD/NAD(P)‑binding
RA 982,560 T→A intergenic (‑122/+481) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
JC JC 2,160,620 IS5 (+) +4 bp intergenic (+110/+160) yegQ → / ← ogrK putative peptidase/positive regulator of P2 growth (insertion of P2 ogr gene into the chromosome)
JC JC 2,400,257 IS5 (+) +4 bp intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
RA 2,420,353 A→C intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
JC JC 3,178,254 IS5 (+) +4 bp coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein
RA 3,370,460 (T)5→4 coding (320/639 nt) sspA ← stringent starvation protein A, phage P1 late gene activator, RNAP‑associated acid‑resistance protein, inactive glutathione S‑transferase homolog
MC JC 4,145,501 Δ320 bp coding (573‑892/1005 nt) argC → N‑acetyl‑gamma‑glutamylphosphate reductase, NAD(P)‑binding

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 2084538 2085913 1376 52 [51] [51] 53 [hisG]–[hisC] [hisG], hisD, [hisC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)164 (1.620) 102/296 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =23 (0.230)75 (0.830) 61/264 0.4 80.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 15 (0.170)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326122 (0.220)53 (0.590) 45/264 1.6 75.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 15 (0.170)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 2420388 =1 (0.010)109 (1.080) 80/296 0.1 99.1% intergenic (‑121/+100) hisJ/argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 242039611 (0.110)100 (0.990) 66/296 0.5 90.1% intergenic (‑129/+92) hisJ/argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA