breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 126,378 G→T 5.7% V228V (GTG→GTT aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,384 C→T 5.4% D230D (GAC→GAT aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,393 C→A 5.1% A233A (GCC→GCA aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 126,405 A→G 6.0% S237S (TCA→TCG aceF → pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
RA 248,263 G→A 5.1% intergenic (+129/+95) rayT → / ← lfhA RAYT REP element‑mobilizing transposase; TnpA(REP)/pseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 289,256 G→C 5.5% intergenic (‑94/+45) yagI ← / ← argF CP4‑6 prophage; putative DNA‑binding transcriptional regulator/ornithine carbamoyltransferase 2, chain F; CP4‑6 prophage
RA 340,050 G→T 5.3% intergenic (+307/‑115) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 340,058 T→C 5.2% intergenic (+315/‑107) yahG → / → yahI DUF1116 family protein/carbamate kinase‑like protein
RA 552,371 G→A 6.1% P75L (CCG→CTG)  purK ← N5‑carboxyaminoimidazole ribonucleotide synthase
RA 617,560 T→C 5.3% L1135P (CTG→CCG)  entF → enterobactin synthase multienzyme complex component, ATP‑dependent
RA 644,030 A→G 6.2% intergenic (‑63/+167) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 644,034 T→C 6.2% intergenic (‑67/+163) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 644,036 A→T 6.7% intergenic (‑69/+161) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 644,038 G→A 6.5% intergenic (‑71/+159) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 644,042 C→T 6.5% intergenic (‑75/+155) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 723,731 A→G 6.0% D228D (GAT→GAC kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
RA 872,398 C→T 5.5% A170V (GCA→GTA)  gsiD → glutathione ABC transporter permease
RA 894,727 C→A 5.6% A315D (GCC→GAC)  potF → putrescine ABC transporter periplasmic binding protein
RA 908,438 T→C 6.1% N286S (AAT→AGT)  ltaE ← L‑allo‑threonine aldolase, PLP‑dependent
RA 944,403 T→C 5.1% V124A (GTT→GCT)  dmsC → dimethyl sulfoxide reductase, anaerobic, subunit C
RA 953,509 T→C 5.0% T16A (ACC→GCC)  pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 991,864 G→A 5.9% A415T (GCA→ACA)  pepN → aminopeptidase N
RA 1,021,175 A→G 5.2% Q13R (CAA→CGA)  sxy → CRP‑S‑dependent promoter expression factor
RA 1,026,727 G→A 5.2% P97S (CCG→TCG)  mgsA ← methylglyoxal synthase
JC JC 1,042,085 IS2 (+) +5 bp 14.6% coding (2122‑2126/2181 nt) etk ← tyrosine‑protein kinase, role in O‑antigen capsule formation
RA 1,129,271 T→C 6.8% L478S (TTA→TCA)  murJ → putative lipid II flippase
RA 1,207,557 C→T 5.8% N19N (AAC→AAT stfP → e14 prophage; uncharacterized protein
RA 1,286,965 A→G 11.1% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,286,971 G→A 10.4% intergenic (+445/+95) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,286,974 C→T 10.1% intergenic (+448/+92) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,286,983 A→G 7.4% intergenic (+457/+83) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,359,878 T→G 6.2% Q344P (CAG→CCG)  puuA ← glutamate‑‑putrescine ligase
RA 1,376,207 C→A 8.0% A58A (GCC→GCA ycjR → putative TIM alpha/beta barrel enzyme
RA 1,376,208 T→G 7.1% C59G (TGC→GGC)  ycjR → putative TIM alpha/beta barrel enzyme
RA 1,466,138 G→A 5.4% pseudogene (747/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,568,915 G→A 6.0% intergenic (‑92/+39) yddW ← / ← gadC liprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter
RA 1,568,917 A→T 5.9% intergenic (‑94/+37) yddW ← / ← gadC liprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter
RA 1,568,919 T→C 5.8% intergenic (‑96/+35) yddW ← / ← gadC liprotein, glycosyl hydrolase homolog/glutamate:gamma‑aminobutyric acid antiporter
RA 1,729,182 T→C 6.0% A32A (GCT→GCC lhr → putative ATP‑dependent helicase
RA 1,764,687 T→C 9.4% intergenic (‑301/+26) sufA ← / ← rydB Fe‑S cluster assembly protein/novel sRNA, function unknown
RA 1,764,693 C→T 9.6% intergenic (‑307/+20) sufA ← / ← rydB Fe‑S cluster assembly protein/novel sRNA, function unknown
RA 1,764,694 G→C 9.6% intergenic (‑308/+19) sufA ← / ← rydB Fe‑S cluster assembly protein/novel sRNA, function unknown
RA 1,764,695 A→G 9.6% intergenic (‑309/+18) sufA ← / ← rydB Fe‑S cluster assembly protein/novel sRNA, function unknown
RA 1,764,701 G→A 9.4% intergenic (‑315/+12) sufA ← / ← rydB Fe‑S cluster assembly protein/novel sRNA, function unknown
RA 1,794,341 T→A 5.8% D271V (GAT→GTT)  btuC ← vitamin B12 ABC transporter permease
RA 1,805,970 G→A 6.3% S38S (AGC→AGT ydiY ← acid‑inducible putative outer membrane protein
JC JC 1,806,297 IS1 (–) +9 bp 100% intergenic (‑214/‑65) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
JC JC 1,806,309 IS5 (–) +4 bp 68.4% intergenic (‑226/‑58) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
RA 1,806,319 C→T 100% intergenic (‑236/‑51) ydiY ← / → pfkB acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
RA 1,806,979 G→A 83.0% D204N (GAT→AAT)  pfkB → 6‑phosphofructokinase II
RA 1,807,169 C→T 5.7% A267V (GCA→GTA)  pfkB → 6‑phosphofructokinase II
RA 1,807,226 C→G 12.1% A286G (GCC→GGC)  pfkB → 6‑phosphofructokinase II
RA 1,808,846 C→A 5.2% G130W (GGG→TGG)  yniB ← putative inner membrane protein
RA 1,825,936 T→A 5.5% intergenic (‑311/+19) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
RA 1,825,937 G→T 5.8% intergenic (‑312/+18) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
RA 1,825,938 A→C 5.8% intergenic (‑313/+17) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
RA 1,825,939 T→A 6.0% intergenic (‑314/+16) spy ← / ← astE periplasmic ATP‑independent protein refolding chaperone, stress‑induced/succinylglutamate desuccinylase
RA 1,829,322 A→C 6.6% L138R (CTG→CGG)  astD ← succinylglutamic semialdehyde dehydrogenase
RA 1,829,328 C→G 6.5% G136A (GGC→GCC)  astD ← succinylglutamic semialdehyde dehydrogenase
RA 1,867,325 G→A 5.0% E140K (GAG→AAG)  yeaG → protein kinase, endogenous substrate unidentified; autokinase
RA 1,927,702 A→T 5.5% S380T (TCT→ACT)  ptrB ← protease II
RA 1,950,085 G→C 5.6% S146R (AGC→AGG aspS ← aspartyl‑tRNA synthetase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 1,982,500 T→C 6.9% intergenic (‑113/+54) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
RA 1,982,501 T→C 6.8% intergenic (‑114/+53) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
RA 1,982,504 G→A 7.0% intergenic (‑117/+50) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
RA 1,982,505 G→A 7.0% intergenic (‑118/+49) otsB ← / ← araH trehalose‑6‑phosphate phosphatase, biosynthetic/L‑arabinose ABC transporter permease
RA 2,074,691 C→G 6.7% intergenic (+33/‑88) flu → / → yeeR CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter/CP4‑44 prophage; putative membrane protein
RA 2,115,547 A→G 5.0% L117P (CTG→CCG)  wcaM ← colanic acid biosynthesis protein
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,149,172 G→A 5.6% A605V (GCG→GTG)  yegI ← protein kinase‑related putative non‑specific DNA‑binding protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,177,566 T→A 5.8% K333N (AAA→AAT fbaB ← fructose‑bisphosphate aldolase class I
RA 2,231,849 T→A 5.6% H2Q (CAT→CAA cdd → cytidine/deoxycytidine deaminase
RA 2,309,028 G→A 5.4% T105M (ACG→ATG)  alkB ← oxidative demethylase of N1‑methyladenine or N3‑methylcytosine DNA lesions
RA 2,346,192 C→T 5.4% A443V (GCC→GTC)  nrdA → ribonucleoside‑diphosphate reductase 1, alpha subunit
RA 2,352,970 T→A 6.7% I108I (ATT→ATA glpA → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, large FAD/NAD(P)‑binding subunit
RA 2,357,653 A→G 8.7% L250L (CTA→CTG yfaD → transposase_31 family protein
RA 2,376,884 C→T 5.1% E237K (GAA→AAA)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,397,483 G→A 5.5% L895L (CTG→TTG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,460,618 C→T 5.2% intergenic (‑149/+32) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
RA 2,560,976 G→T 19.5% intergenic (+78/‑392) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,560,985 C→A 22.5% intergenic (+87/‑383) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,579,154 A→C 5.0% A163A (GCA→GCC talA → transaldolase A
RA 2,653,367 G→A 6.2% intergenic (+28/‑488) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
RA 2,653,372 T→C 5.0% intergenic (+33/‑483) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/novel sRNA, function unknown
RA 2,799,634 A→G 6.0% intergenic (+21/+16) alaE → / ← ygaC alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein
RA 2,799,635 T→A 6.1% intergenic (+22/+15) alaE → / ← ygaC alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein
RA 2,799,636 C→T 6.0% intergenic (+23/+14) alaE → / ← ygaC alanine exporter, alanine‑inducible, stress‑responsive/uncharacterized protein
RA 2,818,324 G→A 5.9% intergenic (‑50/+149) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,947,465 Δ1 bp 14.6% intergenic (+2/‑32) metZ → / → metW tRNA‑Met/tRNA‑Met
RA 2,947,467 A→C 12.4% intergenic (+4/‑30) metZ → / → metW tRNA‑Met/tRNA‑Met
RA 3,324,952 A→G 5.7% intergenic (‑41/+49) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,955 A→G 5.6% intergenic (‑44/+46) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,956 C→T 5.7% intergenic (‑45/+45) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,959 C→T 5.3% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,708,427 G→A 5.5% intergenic (‑722/+189) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,708,428 T→A 6.4% intergenic (‑723/+188) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,708,429 T→C 6.6% intergenic (‑724/+187) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
RA 3,781,051 C→G 7.5% intergenic (+34/‑164) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,972,673 A→C 6.4% E51A (GAG→GCG)  rffG → dTDP‑glucose 4,6‑dehydratase
MC JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,049,986 G→T 6.1% noncoding (88/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
RA 4,049,989 A→C 5.9% noncoding (91/109 nt) spf → Spot 42 sRNA antisense regulator of galK translation, Hfq‑dependent
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,296,060 C→T 33.4% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,409,407 T→A 6.6% S45T (TCT→ACT)  rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,409,410:1 +A 6.7% coding (136/732 nt) rlmB → 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 4,606,239 A→G 18.1% noncoding (48/87 nt) leuP ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423755–3424234 3424531–3424239 6–777 37 [34] [34] 37 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4107541 4108513 973 37 [36] [36] 37 [pfkA] [pfkA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 193444 =78 (0.820)4 (0.050) 4/242 NT 5.2% intergenic (+15/‑77) frr/dxr ribosome recycling factor/1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase
?NC_000913 193489 = 73 (0.840)intergenic (+60/‑32) frr/dxr ribosome recycling factor/1‑deoxy‑D‑xylulose 5‑phosphate reductoisomerase
* ? NC_000913 = 225997NA (NA)3 (0.030) 3/252 NT NA noncoding (239/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 = 226015 NA (NA)noncoding (257/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 1207790 =32 (0.340)34 (0.410) 32/230 NT 55.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 27 (0.330)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780531 (0.330)48 (0.580) 39/230 NT 63.9% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 27 (0.330)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)112 (1.220) 91/254 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1411899 =139 (1.470)20 (0.260) 16/210 NT 15.6% coding (50/936 nt) ttcA tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
?NC_000913 = 1434958 106 (1.400)pseudogene (51/51 nt) ttcC pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related
* ? NC_000913 = 269041160 (0.630)4 (0.050) 4/246 NT 6.6% coding (688/1428 nt) glrK sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
?NC_000913 = 2690416 56 (0.630)coding (683/1428 nt) glrK sensor protein kinase regulating glmY sRNA in two‑component system with response regulator GlrR
* ? NC_000913 3717215 =118 (1.250)6 (0.070) 5/238 NT 5.5% intergenic (+9/‑95) yiaD/ghrB multicopy suppressor of bamB; outer membrane lipoprotein/glyoxylate/hydroxypyruvate reductase B
?NC_000913 3717245 = 101 (1.170)intergenic (+39/‑65) yiaD/ghrB multicopy suppressor of bamB; outer membrane lipoprotein/glyoxylate/hydroxypyruvate reductase B
* ? NC_000913 = 449213158 (0.610)3 (0.030) 3/252 NT 5.1% coding (395/1320 nt) idnT L‑idonate and D‑gluconate transporter
?NC_000913 = 4492165 55 (0.600)coding (361/1320 nt) idnT L‑idonate and D‑gluconate transporter