breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 145 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913417,5330TG44.0% 60.9 / 26.7 77V131G (GTG→GGG) phoBresponse regulator in two‑component regulatory system with PhoR
*NC_0009133,862,6640CG39.3% 30.4 / 26.3 62pseudogene (940/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_000913433,6490TG39.2% 77.7 / 28.9 112G65G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*NC_0009133,745,0010TG38.6% 113.3 / 19.8 91G66G (GGT→GGGyiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
*NC_0009133,745,0190CG38.5% 79.8 / 26.4 78G72G (GGC→GGGyiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
*NC_0009133,862,6670GA37.7% 67.5 / 24.8 61pseudogene (937/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_0009132,684,7790AC36.8% 114.1 / 24.4 100G243G (GGT→GGGglyAserine hydroxymethyltransferase
*NC_0009133,862,6620TG35.5% 60.2 / 13.7 62pseudogene (942/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_0009133,745,3970AC35.4% 211.0 / 25.7 136L198F (TTA→TTCyiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
*NC_000913540,5580TG32.9% 281.4 / 51.4 149intergenic (+50/‑7)allB/ybbYallantoinase/putative uracil/xanthine transporter
*NC_000913249,5380TG30.6% 218.3 / 14.2 135pseudogene (533/1713 nt)lfhApseudogene, flagellar system protein, promoterless fragment; flagellar biosynthesis
*NC_0009133,862,6680AG30.5% 80.1 / 12.8 59pseudogene (936/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_0009134,571,1830AC30.4% 227.2 / 12.5 92V131G (GTG→GGG) yjiRputative DNA‑binding transcriptional regulator/putative aminotransferase
*NC_0009134,223,5970CT29.3% 268.3 / 42.7 134R11K (AGA→AAA) iclRtranscriptional repressor
*NC_0009132,378,9680CT29.3% 117.8 / 31.8 82E98K (GAA→AAA) menD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
*NC_000913354,2880TG29.1% 295.7 / 27.7 110V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_000913354,2820TG28.6% 286.0 / 16.5 105V526G (GTT→GGT) prpEpropionate‑‑CoA ligase
*NC_0009133,862,6700TA28.3% 46.3 / 12.8 63pseudogene (934/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_0009134,361,5310TG28.3% 493.9 / 38.3 194H135P (CAC→CCC) cadCcadBA operon transcriptional activator
*NC_0009131,383,7220GA28.2% 354.4 / 75.9 181F80F (TTC→TTTycjWLacI family putative transcriptional repressor

Marginal new junction evidence (lowest skew 10 of 83 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1207805264 (1.070)55 (0.250) 44/302 3.5 21.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 167 (0.750)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 1207790 =255 (1.040)52 (0.230) 41/302 3.9 20.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 170 (0.760)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 3563404 =228 (0.930)14 (0.060) 12/320 10.5 5.9% coding (1392/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
?NC_000913 3563422 = 225 (0.960)coding (1410/1506 nt) glpD sn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
* ? NC_000913 225456 =68 (0.280)10 (0.050)
+22 bp
7/290 12.0 6.5% noncoding (76/77 nt) ileV tRNA‑Ile
?NC_000913 = 2518117 263 (1.070)intergenic (‑1/+39) alaX/alaW tRNA‑Ala/tRNA‑Ala
* ? NC_000913 624301 =227 (0.920)8 (0.030) 8/316 12.1 3.6% coding (210/957 nt) fepB ferrienterobactin ABC transporter periplasmic binding protein
?NC_000913 624332 = 213 (0.920)coding (179/957 nt) fepB ferrienterobactin ABC transporter periplasmic binding protein
* ? NC_000913 = 3248538282 (1.150)9 (0.040) 8/318 12.2 3.3% coding (100/384 nt) mzrA modulator of EnvZ/OmpR regulon
?NC_000913 = 3248548 267 (1.140)coding (110/384 nt) mzrA modulator of EnvZ/OmpR regulon
* ? NC_000913 = 2518117263 (1.070)10 (0.050)
+22 bp
6/290 12.5 4.2% intergenic (‑1/+39) alaX/alaW tRNA‑Ala/tRNA‑Ala
?NC_000913 = 3427077 NA (NA)noncoding (76/77 nt) ileU tRNA‑Ile
* ? NC_000913 2077808 =317 (1.290)8 (0.040) 7/310 12.5 2.5% coding (240/375 nt) cbtA CP4‑44 prophage; toxin of the YeeV‑YeeU toxin‑antitoxin system
?NC_000913 2077982 = 336 (1.470)pseudogene (43/168 nt) yeeW CP4‑44 prophage; uncharacterized protein;Phage or Prophage Related
* ? NC_000913 3867985 =218 (0.890)7 (0.030) 7/318 12.7 3.2% coding (258/333 nt) yidQ DUF1375 family outer membrane protein
?NC_000913 3868014 = 218 (0.930)coding (287/333 nt) yidQ DUF1375 family outer membrane protein
* ? NC_000913 = 243009202 (0.820)10 (0.040) 7/320 12.7 4.1% coding (295/2445 nt) fadE acyl coenzyme A dehydrogenase
?NC_000913 = 243192 276 (1.170)coding (112/2445 nt) fadE acyl coenzyme A dehydrogenase