breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 579,846 C→A 11.3% intergenic (‑252/‑34) ybcV ← / → ybcW DLP12 prophage; uncharacterized protein/DLP12 prophage; uncharacterized protein
RA 1,413,542 A→T 100% Y67N (TAT→AAT)  ydaC ← DUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
RA 1,793,897 Δ1 bp 100% coding (213/552 nt) btuE ← glutathione peroxidase
RA 1,800,575 G→A 17.3% Q22* (CAG→TAG)  infC ← translation initiation factor IF‑3
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,093,040 G→A 100% A215T (GCT→ACT)  hisC → histidinol‑phosphate aminotransferase
RA 2,516,091 C→T 14.3% M580I (ATG→ATA yfeA ← putative diguanylate cyclase
RA 2,605,627 C→T 8.6% G273G (GGC→GGT hyfD → hydrogenase 4, membrane subunit
RA 2,636,042 A→G 12.0% L438S (TTG→TCG)  der ← GTPase; multicopy suppressor of ftsJ
RA 3,102,557 C→A 17.5% P98P (CCG→CCT trmI ← tRNA m(7)G46 methyltransferase, SAM‑dependent
RA 3,366,973 C→T 8.0% S17L (TCA→TTA)  yhcF → putative transcriptional regulator
RA 3,372,125 T→A 11.9% I150F (ATT→TTT)  nanT ← sialic acid transporter
RA 3,744,794 A→G 9.6% G156G (GGA→GGG yiaM → 2,3‑diketo‑L‑gulonate TRAP transporter small permease protein
RA 3,952,942 A→G 57.1% T154A (ACC→GCC)  ilvE → branched‑chain amino acid aminotransferase
RA 4,117,038 C→A 100% V62L (GTA→TTA)  glpK ← glycerol kinase
RA 4,313,472 C→T 5.7% T41I (ACT→ATT)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,313,777 G→A 5.5% A143T (GCA→ACA)  rpiB → ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
RA 4,357,064 C→A 5.3% D519Y (GAC→TAC)  cadA ← lysine decarboxylase, acid‑inducible
RA 4,450,018 C→T 6.9% A19V (GCG→GTG)  ytfQ → galactofuranose ABC transporter periplasmic binding protein
RA 4,454,128 G→A 6.6% R182H (CGT→CAT)  yjfF → putative sugar ABC transporter permease

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 366111 366455 345 8 [7] [7] 8 [lacZ]–[lacI] [lacZ],[lacI]
* * ÷ NC_000913 3423793–3424232 3424513–3424236 5–721 8 [6] [6] 9 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =5 (0.120)14 (0.370) 14/244 NT 67.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 9 (0.240)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12078055 (0.120)11 (0.290) 11/244 NT 62.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 9 (0.240)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)18 (0.440) 17/268 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 3639287NA (NA)3 (0.070) 3/262 NT NA noncoding (80/98 nt) RIP261 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP261 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 = 3639298 NA (NA)noncoding (91/98 nt) RIP261 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP261 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site