breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation annotation gene description
RA CP006881 30,182 C→T intergenic (+147/‑96) U712_00120 → / → U712_20865 Sigma‑K factor‑processing regulatory protein BofA/16S ribosomal RNA
RA CP006881 125,619 C→T R21C (CGT→TGT)  U712_00560 → DNA‑directed RNA polymerase subunit beta'
RA CP006881 578,474 G→A R61R (CGC→CGT U712_02845 ← UPF0721 transmembrane protein ydhB
RA CP006881 907,728 G→T G70C (GGT→TGT)  U712_04440 → Peroxide operon regulator
RA CP006881 1,666,671 T→A L321* (TTA→TAA)  U712_08575 → Flagellar motor switch phosphatase FliY
RA CP006881 2,801,996 G→A A327T (GCC→ACC)  U712_14440 → UPF0118 membrane protein ytvI
RA CP006881 2,963,948 G→A intergenic (‑135/+145) U712_15285 ← / ← U712_15290 Manganese‑binding lipoprotein mntA/o‑succinylbenzoate synthase
RA CP006881 3,578,472 (A)7→6 intergenic (‑65/‑70) U712_18365 ← / → U712_18370 Stage II sporulation protein Q/Putative phosphinothricin acetyltransferase YwnH
RA CP006881 3,579,791 (A)7→6 coding (79/132 nt) U712_18380 → Hypothetical Protein
RA CP006881 3,723,022 G→A intergenic (‑136/‑165) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,045 T→C intergenic (‑159/‑142) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,067 1 bp→AT intergenic (‑181/‑120) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,091 A→T intergenic (‑205/‑96) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,095 T→A intergenic (‑209/‑92) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,097 T→G intergenic (‑211/‑90) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,118 C→T intergenic (‑232/‑69) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,121 T→A intergenic (‑235/‑66) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,130 A→T intergenic (‑244/‑57) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,162 C→T intergenic (‑276/‑25) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,166 C→G intergenic (‑280/‑21) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,183 A→G intergenic (‑297/‑4) U712_19190 ← / → U712_19195 PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
RA CP006881 3,723,204 G→A L6L (TTG→TTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,210 A→G K8K (AAA→AAG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,222 C→T Y12Y (TAC→TAT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,231 C→A N15K (AAC→AAA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,236 T→A L17Q (CTA→CAA)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,246 T→C F20F (TTT→TTC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,248 T→C V21A (GTT→GCT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,252 T→A S22S (TCT→TCA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,273 2 bp→TC coding (87‑88/771 nt) U712_19195 → putative transporter ywcJ
RA CP006881 3,723,276 A→G R30R (AGA→CGG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,291 C→T S35S (AGC→AGT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,294 G→A M36I (ATG→ATA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,300 C→T I38I (ATC→ATT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,303 A→T G39G (GGA→GGT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,306 C→T F40F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,336 T→C S50S (AGT→AGC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,344 C→T S53F (TCT→TTT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,351 G→T A55A (GCG→GCT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,366 T→C A60A (GCT→GCC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,387 A→T T67T (ACA→ACT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,406 C→A L74M (CTG→ATG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,438 T→C T84T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,447 T→C T87T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,459 T→C T91T (ACT→ACC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,474 A→G R96R (CGA→CGG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,483 2 bp→CA coding (297‑298/771 nt) U712_19195 → putative transporter ywcJ
RA CP006881 3,723,492 A→C R102R (CGA→CGC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,501 G→A L105L (CTG→CTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,505 C→T L107L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,534 G→A L116L (TTG→TTA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,549 A→G L121L (CTA→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,558 T→C I124I (ATT→ATC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,573 A→G T129T (ACA→ACG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,576 G→A G130G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,589 G→C A135P (GCT→CCT)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,616 C→T L144L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,642 C→G P152P (CCC→CCG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,652 C→T L156L (CTG→TTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,666 G→A G160G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,717 T→C L177L (CTT→CTC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,729 G→A G181G (GGG→GGA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,738 C→T L184L (CTC→CTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,771 C→T F195F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,783 C→T F199F (TTC→TTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,822 A→G S212S (TCA→TCG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,853 G→C V223M (GTA→CTG)  U712_19195 → putative transporter ywcJ
RA CP006881 3,723,855 A→G V223M (GTA→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,879 T→C I231I (ATT→ATC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,888 A→G T234T (ACA→ACG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,897 C→T N237N (AAC→AAT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,912 G→A M242I (ATG→ATA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,915 C→T V243V (GTC→GTT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,933 T→C Y249Y (TAT→TAC U712_19195 → putative transporter ywcJ
RA CP006881 3,723,939 G→A T251T (ACG→ACA U712_19195 → putative transporter ywcJ
RA CP006881 3,723,942 C→G L252L (CTC→CTG U712_19195 → putative transporter ywcJ
RA CP006881 3,723,948 C→T P254P (CCC→CCT U712_19195 → putative transporter ywcJ
RA CP006881 3,723,952 A→C K256Q (AAA→CAA)  U712_19195 → putative transporter ywcJ
RA CP006881 3,724,053 C→T E258E (GAG→GAA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,078 C→T S250N (AGT→AAT)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,092 T→C A245A (GCA→GCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,101 A→C L242L (CTT→CTG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,119 A→G F236F (TTT→TTC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,194 A→G S211S (AGT→AGC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,200 T→C A209A (GCA→GCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,208 T→G R207R (AGA→CGA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,274 C→T E185K (GAA→AAA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,392 A→C L145L (CTT→CTG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,446 G→A Y127Y (TAC→TAT U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,467 T→C K120K (AAA→AAG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,542 A→G S95S (TCT→TCC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,587 C→T G80G (GGG→GGA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,623 A→T I68I (ATT→ATA U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,659 A→G F56F (TTT→TTC U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,678 T→C H50R (CAC→CGC)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,719 G→A D36D (GAC→GAT U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,749 T→C P26P (CCA→CCG U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,808 G→A L7L (CTA→TTA)  U712_19200 ← SacPA operon antiterminator
RA CP006881 3,724,831 C→T intergenic (‑5/+35) U712_19200 ← / ← U712_19205 SacPA operon antiterminator/Uncharacterized protein ywcI
RA CP006881 3,724,849 A→T intergenic (‑23/+17) U712_19200 ← / ← U712_19205 SacPA operon antiterminator/Uncharacterized protein ywcI
RA CP006881 3,724,902 A→C A89A (GCT→GCG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,724,977 T→C Q64Q (CAA→CAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,724,980 T→C K63K (AAA→AAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,010 C→T E53E (GAG→GAA U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,025 A→G L48L (CTT→CTC U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,031 C→T A46A (GCG→GCA U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,043 C→A A42A (GCG→GCT U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,049 T→C K40K (AAA→AAG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,082 A→C A29A (GCT→GCG U712_19205 ← Uncharacterized protein ywcI
RA CP006881 3,725,209 +T intergenic (‑41/‑488) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,725,237 A→G intergenic (‑69/‑460) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,725,239 2 bp→AA intergenic (‑71/‑457) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr
RA CP006881 3,892,629 C→T E12K (GAA→AAA)  U712_20070 ← Inosose isomerase
RA pSIJ663_pHT315_araE_xy 2,039 C→T intergenic (–/‑939)  / → eryR –/–
JC pSIJ663_pHT315_araE_xy 2,655 Δ3 bp intergenic (–/‑321)  / → eryR –/–
JC pSIJ663_pHT315_araE_xy 3,843 Δ5 bp intergenic (+128/‑24) eryR → / → ampR –/–
RA pSIJ663_pHT315_araE_xy 5,049 C→T intergenic (+318/–) ampR → /  –/–
RA pSIJ663_pHT315_araE_xy 5,205 G→A intergenic (+474/–) ampR → /  –/–
RA pSIJ663_pHT315_araE_xy 5,478 G→A intergenic (+747/–) ampR → /  –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP006881 245170 246099 930 47 [26] [28] 29 [U712_01140]–[U712_01145] [U712_01140],[U712_01145]
* * ÷ CP006881 1067434 1068642 1209 30 [27] [24] 29 U712_05250 U712_05250
* * ÷ CP006881 1149053 1150720 1668 31 [26] [28] 30 U712_05685 U712_05685
* * ÷ CP006881 1503058 1504650 1593 30 [27] [26] 29 U712_07740 U712_07740
* * ÷ CP006881 1562243 1566625 4383 29 [28] [27] 32 U712_08060 U712_08060
* * ÷ CP006881 2261293 2262067 775 29 [21] [23] 29 U712_11400 U712_11400
* * ÷ CP006881 3303135–3303322 3304615 1294–1481 31 [28] [1] 47 [U712_16990]–U712_16995 [U712_16990],U712_16995
* * ÷ CP006881 3725697 3728128 2432 34 [27] [26] 30 [U712_19210] [U712_19210]
* * ÷ CP006881 3757680 3759663 1984 30 [26] [28] 30 U712_19375 U712_19375

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP006881 390833 =73 (0.660)116 (1.050) 57/252 0.2 56.2% coding (3098/10752 nt) U712_01810 Surfactin synthase subunit 2
?CP006881 = 1080730 108 (0.980)intergenic (+9/+38) U712_05320/U712_05325 Competence transcription factor/putative oxidoreductase yhxD
* ? CP006881 = 449675102 (0.930)111 (1.050) 46/242 0.5 57.7% intergenic (+132/‑1935) U712_02060/U712_02070 Uncharacterized protein ycnL/Mannitol‑1‑phosphate 5‑dehydrogenase
?CP006881 1080118 = 65 (0.620)intergenic (‑268/‑25) U712_05315/U712_05320 Uncharacterized protein yhzC/Competence transcription factor
* ? CP006881 2228866 =126 (1.150)46 (0.430) 25/246 1.9 42.6% intergenic (‑118/‑88) U712_11195/U712_11200 Uncharacterized protein ypzE/D‑3‑phosphoglycerate dehydrogenase
?CP006881 3304616 = 1 (0.010)intergenic (+4/+45) U712_16995/U712_17000 Arabinose metabolism transcriptional repressor/Uncharacterized protein yvbT
* ? CP006881 = 2228918140 (1.270)80 (0.050) 26/252 1.9 36.4% intergenic (‑170/‑36) U712_11195/U712_11200 Uncharacterized protein ypzE/D‑3‑phosphoglycerate dehydrogenase
?pSIJ663_pHT315_araE_xy 9781 = NA (NA)intergenic (+5050/–) ampR/– –/–