breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 54 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,0600CT31.3% 254.1 / 168.4 195intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009134,051,2590GT25.9% 271.8 / 102.5 131noncoding (224/245 nt)csrCCsrC sRNA sequesters CsrA, a carbon flux regulator
*NC_0009132,800,5190TG24.8% 224.9 / 85.1 117intergenic (+44/‑204)ygaM/nrdHputative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
*NC_0009134,051,2620AC24.1% 293.4 / 104.0 141noncoding (227/245 nt)csrCCsrC sRNA sequesters CsrA, a carbon flux regulator
*NC_0009133,171,8630AG23.8% 242.9 / 82.7 130intergenic (+30/+16)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
*NC_0009132,286,3130TA23.7% 273.8 / 88.1 137intergenic (+26/+77)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
*NC_0009133,202,6860CT23.7% 340.7 / 106.3 173L266F (CTC→TTC) ccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
*NC_0009131,865,6820GC23.7% 373.1 / 120.7 169intergenic (‑46/+44)yeaE/mipAaldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
*NC_0009134,307,2900TC23.5% 246.8 / 76.9 133G170G (GGA→GGGalsKD‑allose kinase
*NC_0009133,202,6850AG23.4% 339.7 / 108.8 175E265E (GAA→GAGccafused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
*NC_000913120,1540T.23.4% 356.5 / 105.7 124intergenic (+19/+24)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
*NC_0009132,286,3110CG23.4% 304.2 / 97.2 137intergenic (+24/+79)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
*NC_0009132,286,3150CG23.4% 308.1 / 97.4 137intergenic (+28/+75)proL/yejOtRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
*NC_0009132,800,5150CA23.3% 251.7 / 75.3 116intergenic (+40/‑208)ygaM/nrdHputative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein
*NC_000913120,1581.A23.2% 300.6 / 131.7 125intergenic (+23/+20)ampE/aroPampicillin resistance inner membrane protein; putative signaling protein in beta‑lactamase regulation/aromatic amino acid transporter
*NC_0009132,356,7370TC23.2% 241.7 / 73.5 125intergenic (+26/‑167)glpC/yfaDanaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster/transposase_31 family protein
*NC_0009133,171,8520CT23.1% 262.9 / 77.1 131intergenic (+19/+27)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
*NC_0009131,674,9580AT22.9% 276.8 / 85.4 131intergenic (+11/+14)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
*NC_0009132,323,2800AG22.9% 362.2 / 94.5 166intergenic (+29/‑167)atoC/atoDfused response regulator of ato operon, in two‑component system with AtoS: response regulator/sigma54 interaction protein/acetyl‑CoA:acetoacetyl‑CoA transferase, alpha subunit
*NC_0009132,800,5170TA22.9% 243.7 / 78.1 118intergenic (+42/‑206)ygaM/nrdHputative membrane‑anchored DUF883 family ribosome‑binding protein/hydrogen donor for NrdEF electron transport system; glutaredoxin‑like protein

Marginal new junction evidence (lowest skew 10 of 1256 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1468681NA (NA)109 (1.540)
+44 bp
15/110 5.6 NA noncoding (560/1331 nt) IS2 repeat region
?NC_000913 1650843 = NA (NA)noncoding (1/706 nt) IS2 repeat region
* ? NC_000913 381819 =NA (NA)45 (0.380) 16/184 10.9 NA noncoding (560/1331 nt) IS2 repeat region
?NC_000913 381841 = NA (NA)noncoding (582/1331 nt) IS2 repeat region
* ? NC_000913 = 3718305116 (0.900)21 (0.180) 15/182 11.2 15.9% intergenic (+21/+29) ghrB/yiaF glyoxylate/hydroxypyruvate reductase B/barrier effect co‑colonization resistance factor; DUF3053 family lipoprotein
?NC_000913 = 3718325 115 (0.970)intergenic (+41/+9) ghrB/yiaF glyoxylate/hydroxypyruvate reductase B/barrier effect co‑colonization resistance factor; DUF3053 family lipoprotein
* ? NC_000913 = 294746794 (0.730)12 (0.160)
+40 bp
6/118 11.3 15.6% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 = 2947757 125 (0.970)coding (1254/1254 nt) amiC N‑acetylmuramoyl‑L‑alanine amidase
* ? NC_000913 = 3546221NA (NA)30 (0.250) 15/186 11.5 NA intergenic (+22/‑338) nfuA/gntT Fe/S biogenesis protein, putative scaffold/chaperone protein/gluconate transporter, high‑affinity GNT I system
?NC_000913 = 3546253 NA (NA)noncoding (32/36 nt) REP253 (repetitive extragenic palindromic) element; contains 1 REP sequences REP253 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? NC_000913 = 2464223141 (1.100)20 (0.180) 13/172 11.5 13.6% intergenic (+153/+29) yfdF/mlaA uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
?NC_000913 = 2464229 132 (1.180)intergenic (+159/+23) yfdF/mlaA uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
* ? NC_000913 = 227444NA (NA)24 (0.200) 15/188 11.6 NA noncoding (1686/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 = 227459 NA (NA)noncoding (1701/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 225889 =NA (NA)45 (0.380) 14/184 11.9 NA noncoding (131/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 225907 = NA (NA)noncoding (149/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 = 315646NA (NA)32 (0.260) 14/188 12.1 NA noncoding (418/1255 nt) IS3 repeat region
?NC_000913 = 315665 NA (NA)noncoding (437/1255 nt) IS3 repeat region
* ? NC_000913 2728214 =NA (NA)52 (0.420) 14/190 12.3 NA noncoding (971/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
?NC_000913 2728240 = NA (NA)noncoding (945/2904 nt) rrlG 23S ribosomal RNA of rrnG operon