breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 838,384 G→T R25S (CGC→AGC)  ybiI ← zinc finger domain‑containing protein YbiI
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,787,765 T→G D107E (GAT→GAG ppsR → posphoenolpyruvate synthetase regulatory protein
RA 1,822,111 +G coding (146/339 nt) osmE ← osmotically‑inducible lipoprotein OsmE
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,178,530 G→T A12D (GCC→GAC)  fbaB ← fructose‑bisphosphate aldolase class I
RA 2,215,840 C→A V213L (GTG→TTG)  yehW ← glycine betaine ABC transporter membrane subunit YehW
RA 2,654,221 G→A A238V (GCG→GTG)  sseB ← protein SseB
RA 2,857,685 G→T R198L (CGT→CTT)  mutS → DNA mismatch repair protein MutS
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,550,906 A→T I523N (ATC→AAC)  malP ← maltodextrin phosphorylase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,622,433 C→A S1081Y (TCC→TAC)  rhsB → rhs element protein RhsB
RA 3,699,141 G→T D310Y (GAT→TAT)  bcsG → cellulose phosphoethanolamine transferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,053,884 C→A E465D (GAG→GAT glnG ← DNA‑binding transcriptional dual regulator NtrC
RA 4,208,051 G→T intergenic (‑519/‑96) purH ← / → rrsE bifunctional AICAR transformylase/IMP cyclohydrolase/16S ribosomal RNA
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,446,230 C→A L707I (CTT→ATT)  tamB → translocation and assembly module subunit TamB
RA 4,547,721 G→T E876* (GAA→TAA)  fimD → type I fimbriae usher protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102972 3104074 1103 50 [44] [44] 45 mutY mutY
* * ÷ NC_000913 3423755–3424234 3424540–3424239 6–786 47 [41] [41] 46 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 5743351 (0.010)235 (1.540) 103/276 0.0 99.6% coding (380/384 nt) ybcQ DLP12 prophage; putative antitermination protein
?NC_000913 574591 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1207790 =166 (1.080)59 (0.440) 47/244 1.2 27.9% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 159 (1.180)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 1207805166 (1.080)47 (0.350) 34/244 2.2 23.5% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 159 (1.180)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)188 (1.270) 88/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =191 (1.250)265 (1.740) 97/274 0.1 73.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)137 (0.900) 80/276 0.3 93.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA