breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 963,152 C→A G386G (GGC→GGA rpsA → 30S ribosomal subunit protein S1
RA 1,378,900 G→A L339L (TTG→TTA ycjT → kojibiose phosphorylase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,798,834 G→T H37N (CAC→AAC)  pheS ← phenylalanine‑‑tRNA ligase subunit alpha
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,891,299 C→A intergenic (‑32/+26) tsaB ← / ← yoaA N(6)‑L‑threonylcarbamoyladenine synthase, TsaB subunit/putative 5' to 3' DNA helicase implicated in DNA repair
RA 1,913,560 G→T V419V (GTC→GTA prc ← tail‑specific protease
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,323,339 C→A intergenic (+88/‑108) atoC → / → atoD DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC/acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
RA 2,473,155 G→T P31Q (CCG→CAG)  yfdO ← CPS‑53 (KpLE1) prophage; putative defective phage replication protein O
RA 2,937,463 C→A intergenic (+104/‑5) fucI → / → fucK L‑fucose isomerase/L‑fuculokinase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,857,150 G→T R419S (CGT→AGT)  yidJ ← putative sulfatase/phosphatase
RA 3,865,065 C→A A403A (GCG→GCT yidE ← putative transport protein YidE
RA 3,933,833 C→A A19D (GCC→GAC)  rbsA → ribose ABC transporter ATP binding subunit
RA 3,990,203 T→C P188P (CCA→CCG hemC ← hydroxymethylbilane synthase
RA 4,224,881 G→T E352* (GAA→TAA)  metH → cobalamin‑dependent methionine synthase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,316,106 C→A W371C (TGG→TGT phnM ← RPnTP hydrolase
RA 4,405,335 G→T D217Y (GAT→TAT)  purA → adenylosuccinate synthetase
RA 4,534,255 G→T intergenic (‑202/+175) topAI ← / ← yjhZ KpLE2 phage‑like element; toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/KpLE2 phage‑like element; putative acetyltransferase YjhZ

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102978 3104043 1066 5 [3] [0] 4 [mutY] [mutY]
* * ÷ NC_000913 3423780–3424524 3424524 1–745 4 [3] [3] 4 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =11 (0.400)48 (1.980) 38/244 0.0 85.9% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 6 (0.250)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780511 (0.400)14 (0.580) 13/244 0.7 64.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 6 (0.250)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)37 (1.390) 30/268 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =48 (1.750)35 (1.290) 25/274 0.2 58.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.070)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)36 (1.320) 28/276 0.1 94.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 4373285NA (NA)3 (0.130) 3/226 2.6 NA noncoding (12/34 nt) other REP328b
?NC_000913 = 4374507 NA (NA)noncoding (9/34 nt) other REP329a