breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 87,336 C→A S569R (AGC→AGA ilvI → acetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
RA 141,380 C→A intergenic (‑155/‑51) gcd ← / → hpt quinoprotein glucose dehydrogenase/hypoxanthine phosphoribosyltransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC 335,047 IS1 (+) +9 bp intergenic (‑25/‑225) yahC ← / → yahD uncharacterized protein YahC/ankyrin repeat‑containing protein YahD
RA 772,783 C→A F442L (TTC→TTA cydA → cytochrome bd‑I ubiquinol oxidase subunit I
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,097,052 C→A intergenic (+223/+513) ycdU → / ← serX uncharacterized protein YcdU/tRNA‑Ser
RA 1,116,917 T→C D37D (GAT→GAC yceA → UPF0176 protein YceA
RA 1,197,425 G→T S36R (AGC→AGA ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,361,714 C→A R199S (CGC→AGC)  puuD → gamma‑glutamyl‑gamma‑aminobutyrate hydrolase
RA 1,439,379 C→A R469L (CGA→CTA)  ydbK ← putative pyruvate‑flavodoxin oxidoreductase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,702,155 G→T intergenic (+26/‑78) malY → / → add negative regulator of MalT activity/cystathionine beta‑lyase/adenosine deaminase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,241,572 G→T R33S (CGT→AGT)  galS ← DNA‑binding transcriptional dual regulator GalS
RA 2,730,953 T→C noncoding (205/1542 nt) rrsG ← 16S ribosomal RNA
RA 3,305,374 G→T A148A (GCC→GCA mtr ← tryptophan:H(+) symporter Mtr
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,696,654 C→A A66E (GCG→GAG)  bcsE → c‑di‑GMP‑binding protein BcsE
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102949 3104054 1106 51 [48] [48] 49 [mutY] [mutY]
* * ÷ NC_000913 3423766–3424525 3424525 1–760 50 [48] [34] 53 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =39 (0.210)274 (1.690) 91/244 0.1 87.9% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 41 (0.250)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780539 (0.210)121 (0.750) 59/244 0.7 76.2% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 41 (0.250)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)322 (1.810) 110/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =297 (1.620)303 (1.670) 97/274 0.1 66.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 8 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)208 (1.140) 85/276 0.3 95.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 11 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA