breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 300,144 C→A R264L (CGT→CTT)  paoC ← aldehyde dehydrogenase: molybdenum cofactor‑binding subunit
RA 364,052 G→T L752M (CTG→ATG)  lacZ ← beta‑galactosidase
RA 463,976 C→A L14M (CTG→ATG)  ybaV → helix‑hairpin‑helix 3 family protein
RA 497,660 G→T E162D (GAG→GAT adk → adenylate kinase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 938,200 G→T V69V (GTG→GTT rarA → recombination factor
RA 946,335 C→A T155T (ACC→ACA ycaD → putative transporter YcaD
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,738,525 C→A R228S (CGC→AGC)  purR → DNA‑binding transcriptional repressor PurR
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,599,317 C→A V148V (GTG→GTT dapA ← 4‑hydroxy‑tetrahydrodipicolinate synthase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,919,999 G→T R135R (CGC→CGA atpH ← ATP synthase F1 complex subunit delta
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102972 3104052 1081 24 [23] [23] 26 [mutY] [mutY]
* * ÷ NC_000913 3423767–3424234 3424526–3424239 6–760 24 [22] [23] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =20 (0.230)106 (1.390) 59/244 0.1 83.6% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 24 (0.310)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780520 (0.230)64 (0.840) 47/244 0.4 75.4% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 24 (0.310)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)84 (1.000) 55/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =97 (1.120)132 (1.530) 65/274 0.1 72.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)95 (1.100) 58/276 0.2 97.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA