breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 38,132 C→A L328L (CTG→CTT caiB ← gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC 490,962 (GTATTACCG)2→3 coding (81/378 nt) ybaN → conserved inner membrane protein YbaN
JC JC 568,908 IS2 (–) +5 bp coding (7‑11/1527 nt) ybcK → DLP12 prophage; putative recombinase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 951,454 C→T E701K (GAA→AAA)  pflB ← pyruvate formate‑lyase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,449,784 C→A V522V (GTG→GTT tynA ← copper‑containing amine oxidase
JC JC 1,580,223 IS5 (–) +4 bp coding (565‑568/1158 nt) ydeM ← putative anaerobic sulfatase maturation enzyme YdeM
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,154,736 C→G P241A (CCG→GCG)  mdtA → multidrug efflux pump membrane fusion protein MdtA
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,505,811 G→C R274R (CGC→CGG ypdF ← aminopeptidase
RA 2,734,747 G→T P96P (CCC→CCA pgeF ← polyphenol oxidase YfiH
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,588,006 C→A K36N (AAG→AAT ugpQ ← glycerophosphodiester phosphodiesterase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,941,766 G→T intergenic (‑439/‑42) yieP ← / → rrsC putative transcriptional regulator YieP/16S ribosomal RNA
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
MC 4,326,086 Δ153 bp between other yjdN ← / ← yjdM conserved protein YjdN/conserved protein YjdM
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196275 1211428 15154 13 [9] [10] 12 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3103013 3104051 1039 11 [9] [9] 11 mutY adenine DNA glycosylase
* * ÷ NC_000913 3423782–3424234 3424531–3424239 6–750 12 [10] [10] 11 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374922 =NA (NA)41 (0.850) 14/258 2.0 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377329 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 374922 =NA (NA)18 (0.480) 8/200 2.4 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377430 = NA (NA)intergenic (+119/+105) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)51 (1.450) 16/188 0.9 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375160NA (NA)32 (0.630)
+T
14/272 2.1 NA noncoding (28/34 nt) other REP31e
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 377321 =NA (NA)27 (0.730)
+38 bp
9/198 2.2 NA intergenic (+10/+214) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
?NC_000913 2347281 = NA (NA)noncoding (34/34 nt) other REP164c
* ? NC_000913 608007 =NA (NA)20 (0.480) 12/222 1.9 26.0% noncoding (1/1345 nt) IS186 repeat region
?NC_000913 609352 = 57 (1.370)intergenic (+175/+107) insL‑2/entD IS186/IS421 transposase/phosphopantetheinyl transferase EntD
* ? NC_000913 = 12994980 (0.000)58 (1.160) 38/266 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =65 (1.260)68 (1.330) 45/272 0.1 67.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)55 (1.070) 41/274 0.2 94.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4373295 =NA (NA)21 (0.440) 11/254 2.5 NA noncoding (2/34 nt) other REP328b
?NC_000913 4374499 = NA (NA)noncoding (1/34 nt) other REP329a