breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 382,816 A→C pseudogene (57/282 nt)
Y374D (TAT→GAT) 
yaiX ←
yaiP ←
putative acyltransferase, N‑terminal fragment
putative glucosyltransferase
RA 627,886 G→T D65Y (GAC→TAC)  entB → enterobactin synthase component B
RA 729,580 Δ1 bp intergenic (+240/‑3) ybfA → / → rhsC DUF2517 domain‑containing protein YbfA/rhs element protein RhsC
RA 729,582 G→A intergenic (+242/‑1) ybfA → / → rhsC DUF2517 domain‑containing protein YbfA/rhs element protein RhsC
RA 747,037 G→T L245I (CTT→ATT)  abrB ← putative regulator
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,116,467 C→G R39P (CGC→CCC)  lpxL ← lauroyl acyltransferase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,229,655 A→G I247T (ATA→ACA)  hlyE ← hemolysin E
MC JC 1,565,215 Δ18 bp coding (502‑519/2400 nt) dosP ← oxygen‑sensing c‑di‑GMP phosphodiesterase DosP
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,560,986 A→C intergenic (+88/‑382) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein YffL/CPZ‑55 prophage; uncharacterized protein YffM
RA 3,011,485 C→A L9M (CTG→ATG)  yqeA → putative amino acid kinase YqeA
RA 3,062,031 A→T G394G (GGA→GGT scpA → methylmalonyl‑CoA mutase
RA 3,234,465 C→A intergenic (‑10/+274) higB ← / ← rlmG ribosome‑dependent mRNA interferase toxin HigB/23S rRNA m(2)G1835 methyltransferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,792,715 C→T intergenic (‑164/+111) kbl ← / ← htrL 2‑amino‑3‑ketobutyrate CoA ligase/protein HtrL
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,918,912 C→A E316* (GAA→TAA)  atpA ← ATP synthase F1 complex subunit alpha
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 729599–729631 729760–729715 85–162 20 [17] [18] 22 rhsC rhs element protein RhsC
* * ÷ NC_000913 1196280 1211433 15154 21 [18] [18] 21 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102994 3104051 1058 21 [18] [16] 20 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424528–3424238 5–759 19 [17] [18] 19 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)149 (1.800) 77/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =174 (2.040)139 (1.640) 73/274 0.0 61.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)132 (1.550) 75/276 0.0 96.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA