breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 442,961 G→A E471E (GAG→GAA thiI → tRNA uridine 4‑sulfurtransferase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,649,867 T→G V25G (GTT→GGT)  ydfD → Qin prophage; lysis protein
RA 1,802,309 G→C P88A (CCG→GCG)  thrS ← threonine‑‑tRNA ligase
RA 1,863,500 A→G T244A (ACC→GCC)  gapA → glyceraldehyde‑3‑phosphate dehydrogenase A
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,616,162 C→A A23A (GCC→GCA bepA → beta‑barrel assembly‑enhancing protease
RA 2,653,971 T→G noncoding (117/304 nt) ryfA → small regulatory RNA RyfA
RA 2,886,861 T→C Y120C (TAT→TGT)  cas3 ← CRISPR‑associated endonuclease/helicase Cas3
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,768,355 C→T S60L (TCA→TTA)  yibG → tetratricopeptide‑like domain‑containing protein YibG
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,835,418 T→C N171S (AAT→AGT)  yicJ ← putative xyloside transporter YicJ
RA 3,847,546 C→G G360A (GGC→GCC)  uhpC ← inner membrane protein sensing glucose‑6‑phosphate
RA 4,279,622 T→G F382V (TTT→GTT)  ghxP → guanine/hypoxanthine transporter GhxP
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196255 1211452 15198 26 [25] [25] 26 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 2818134–2818141 2818259–2818201 61–126 26 [25] [24] 28 [argZ]–[argY] [argZ],[argY]
* * ÷ NC_000913 3423783–3424234 3424530–3424239 6–748 26 [25] [24] 29 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =106 (1.290)135 (1.660) 70/274 0.0 71.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)87 (1.060) 58/276 0.2 97.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA