breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 354,503 G→A A600T (GCG→ACG)  prpE → propionyl‑CoA synthetase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,003,448 A→C R53R (CGT→CGG fliC ← flagellar filament structural protein
JC JC 2,169,898 IS1 (+) +9 bp pseudogene (262‑270/475 nt) gatR ← DNA‑binding transcriptional repressor GatR, N‑terminal fragment
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,617,797 G→C A74P (GCT→CCT)  yfgD → putative oxidoreductase YfgD
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
JC 3,447,581 +GGC coding (187/315 nt) rplX ← 50S ribosomal subunit protein L24
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,647,083 G→A G262D (GGT→GAT)  gor → glutathione reductase (NADPH)
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,892,014 G→T G134C (GGT→TGT)  mdtL → efflux pump MdtL
MC JC 4,082,677 Δ204 bp [fdoH][fdoG] [fdoH], [fdoG]
RA 4,274,902 C→T G47D (GGC→GAC)  yjcB ← uncharacterized protein YjcB
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,623,281 Δ2 bp coding (836‑837/1017 nt) lplA ← lipoate‑‑protein ligase A

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196260 1211431 15172 25 [24] [22] 25 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423770–3424234 3424530–3424238 5–761 27 [24] [24] 25 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)76 (0.770) 47/268 0.6 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2481751 =63 (0.620)49 (0.490) 28/270 1.8 36.1% coding (426/1539 nt) emrY tripartite efflux pump membrane subunit EmrY
?NC_000913 = 2481901 112 (1.130)coding (276/1539 nt) emrY tripartite efflux pump membrane subunit EmrY
* ? NC_000913 3363001 =177 (1.750)171 (1.700) 73/274 0.1 65.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.050)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)111 (1.100) 58/276 0.3 95.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 5 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA