breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 667,643 T→C N192S (AAT→AGT)  mrdA ← peptidoglycan DD‑transpeptidase MrdA
RA 684,941 C→T V105V (GTG→GTA gltL ← glutamate/aspartate ABC transporter ATP binding subunit
MC JC 716,842 Δ10 bp intergenic (‑245/+95) ybfG ← / ← potE PF10964 domain‑containing protein YbfG/putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
RA 838,498 T→G intergenic (‑42/+32) ybiI ← / ← ybiX zinc finger domain‑containing protein YbiI/PKHD‑type hydroxylase YbiX
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,102,028 T→C N34D (AAC→GAC)  csgF ← curli assembly component
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,193,328 G→A H281Y (CAT→TAT)  mrp ← P‑loop NTPase family protein
RA 2,696,638 +AT coding (838/1557 nt) mltF → membrane‑bound lytic murein transglycosylase F
JC 2,816,330 (TGTGC)1→2 coding (110/1557 nt) gshA ← glutamate‑‑cysteine ligase
JC 3,277,273 (TTCAACA)2→3 coding (272/336 nt) prlF → antitoxin PrlF
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,371,175 A→T L467* (TTG→TAG)  nanT ← N‑acetylneuraminate:H(+) symporter
RA 3,428,558 T→C noncoding (205/1542 nt) rrsD ← 16S ribosomal RNA
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1096995 1097569 575 51 [1] [15] 16 [serX] [serX]
* * ÷ NC_000913 1196257 1211438 15182 17 [15] [15] 18 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 1552114–1552186 1552271–1552213 28–158 17 [13] [15] 16 fdnI/yddM formate dehydrogenase N subunit gamma/putative DNA‑binding transcriptional regulator YddM
* * ÷ NC_000913 3423770–3424233 3424552–3424239 7–783 19 [15] [15] 16 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 4035266–4035733 4036975–4035735 3–1710 16 [15] [14] 17 [rrsA] [rrsA]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 925857 =39 (0.670)17 (0.440) 8/184 2.8 56.8% intergenic (+317/+27) clpA/serW ATP‑dependent Clp protease ATP‑binding subunit ClpA/tRNA‑Ser
?NC_000913 = 1096994 0 (0.000)intergenic (+165/+571) ycdU/serX uncharacterized protein YcdU/tRNA‑Ser
* ? NC_000913 = 12994980 (0.000)54 (0.960) 37/268 0.4 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =70 (1.200)97 (1.680) 55/274 0.1 73.4% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)61 (1.050) 39/276 0.4 98.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 454269028 (0.480)14 (0.260) 14/258 2.5 28.8% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 43 (0.790)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit