breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 35,814 Δ1 bp 41.0% intergenic (+45/+63) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,817 A→T 38.8% intergenic (+48/+60) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,850 C→T 51.3% intergenic (+81/+27) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,858 T→A 51.5% intergenic (+89/+19) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,876 C→T 46.4% intergenic (+107/+1) rlmH → / ← tpn hypothetical protein/IS431mec transposase
RA 35,913 C→G 15.6% S213S (TCG→TCC tpn ← IS431mec transposase
RA 35,940 G→A 19.2% R204R (CGC→CGT tpn ← IS431mec transposase
RA 35,970 T→C 63.8% E194E (GAA→GAG tpn ← IS431mec transposase
RA 35,997 T→C 28.7% T185T (ACA→ACG tpn ← IS431mec transposase
RA 36,015 C→T 29.1% R179R (AGG→AGA tpn ← IS431mec transposase
RA 36,087 A→G 56.6% C155C (TGT→TGC tpn ← IS431mec transposase
RA 36,119 C→T 30.2% V145I (GTA→ATA)  tpn ← IS431mec transposase
RA 36,124 G→A 58.6% A143V (GCT→GTT)  tpn ← IS431mec transposase
RA 36,133 A→G 29.5% V140A (GTA→GCA)  tpn ← IS431mec transposase
RA 36,161 T→C 28.1% I131V (ATT→GTT)  tpn ← IS431mec transposase
RA 36,174 T→C 25.2% K126K (AAA→AAG tpn ← IS431mec transposase
RA 36,269 C→T 59.8% A95T (GCA→ACA)  tpn ← IS431mec transposase
RA 36,315 G→A 57.8% Y79Y (TAC→TAT tpn ← IS431mec transposase
RA 36,378 C→T 93.0% L58L (TTG→TTA tpn ← IS431mec transposase
RA 36,413 C→T 51.8% V47I (GTC→ATC)  tpn ← IS431mec transposase
RA 36,444 C→T 49.2% R36R (AGG→AGA tpn ← IS431mec transposase
RA 36,479 T→C 28.0% T25A (ACA→GCA)  tpn ← IS431mec transposase
RA 36,501 G→A 15.0% A17A (GCC→GCT tpn ← IS431mec transposase
RA 36,581 C→T 29.6% intergenic (‑30/‑493) tpn ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein
RA 40,391 G→T 11.1% A248E (GCG→GAG)  mecA ← penicillin binding protein 2 prime
RA 76,180 C→T 38.3% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,185 G→A 14.6% intergenic (+243/‑891) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,265 C→T 26.6% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,295 T→C 60.2% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,322 G→A 59.5% intergenic (+380/‑754) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 76,353 G→A 52.8% intergenic (+411/‑723) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase
RA 111,512 A→G 19.4% S194S (TCA→TCG USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 111,516 C→A 20.0% Q196K (CAA→AAA)  USA300HOU_0110 → possible staphylococcal tandem lipoprotein
RA 306,351 C→A 14.5% A119E (GCG→GAG)  lytS → sensor histidine kinase LytS
RA 826,574 C→T 18.1% D168N (GAT→AAT)  yvyE ← hypothetical membrane protein
RA 847,926 T→C 14.8% A289A (GCT→GCC trxB1 → thioredoxin‑disulfide reductase
RA 1,009,940 G→A 14.2% R73H (CGT→CAT)  rluA1 → pseudouridylate synthase
RA 1,221,684 C→A 19.6% intergenic (+99/‑111) rpoZ → / → rpoZ DNA‑directed RNA polymerase subunit omega/bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase
RA 1,223,605 C→T 22.3% Y202Y (TAC→TAT priA → primosomal protein N prime
RA 1,416,458 A→T 100% I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,459,907 T→C 13.8% K21R (AAA→AGA)  USA300HOU_1356 ← possible acetyltransferase
RA 1,513,930 A→T 100% L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C 100% A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,929,796 T→C 33.3% intergenic (‑879/‑289) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,797 G→A 34.1% intergenic (‑880/‑288) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,799 C→A 33.9% intergenic (‑882/‑286) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,804 A→G 33.1% intergenic (‑887/‑281) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,812 G→A 40.4% intergenic (‑895/‑273) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,843 Δ1 bp 56.5% intergenic (‑926/‑242) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,848 C→A 56.2% intergenic (‑931/‑237) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,861 T→C 54.7% intergenic (‑944/‑224) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,862:1 +T 43.8% intergenic (‑945/‑223) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,865 A→C 44.0% intergenic (‑948/‑220) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,866 G→T 52.9% intergenic (‑949/‑219) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,867 G→T 52.1% intergenic (‑950/‑218) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,870 A→C 54.7% intergenic (‑953/‑215) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,872 G→A 54.7% intergenic (‑955/‑213) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,873 C→A 54.7% intergenic (‑956/‑212) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,875 T→C 53.9% intergenic (‑958/‑210) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,917 C→T 46.5% intergenic (‑1000/‑168) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,918 A→G 44.9% intergenic (‑1001/‑167) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,925 T→A 36.6% intergenic (‑1008/‑160) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,928 T→A 36.6% intergenic (‑1011/‑157) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,930 T→A 37.1% intergenic (‑1013/‑155) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,941 A→T 32.9% intergenic (‑1024/‑144) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 1,929,946 G→A 32.8% intergenic (‑1029/‑139) USA300HOU_1794 ← / → USA300HOU_1796 hypothetical protein/hypothetical protein
RA 2,010,363 C→T 100% C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,096,684 A→G 22.1% S246P (TCT→CCT)  USA300HOU_1958 ← hypothetical bacteriophage protein
RA 2,104,016 T→C 13.4% K98K (AAA→AAG USA300HOU_1964 ← hypothetical bacteriophage protein
RA 2,138,686 A→T 14.5% intergenic (‑34/+284) USA300HOU_2020 ← / ← xtmA hypothetical membrane protein/terminase small subunit
RA 2,146,374 T→C 24.0% L183S (TTA→TCA)  orf5 → amidohydrolase
RA 2,187,071 C→T 100% R331K (AGA→AAA)  rsbU ← sigma factor B regulator
RA 2,279,705 G→A 100% intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,306,533 A→G 13.8% P368P (CCT→CCC lmrS ← hypothetical membrane protein
RA 2,391,573 Δ1 bp 16.0% coding (169/447 nt) moaE ← molybdopterin synthase large subunit
RA 2,391,574 A→T 16.0% A56A (GCT→GCA moaE ← molybdopterin synthase large subunit
RA 2,403,678 A→T 16.0% V185D (GTT→GAT)  USA300HOU_2267 ← acyl‑CoA dehydrogenase
RA 2,510,547 G→A 13.8% intergenic (+669/‑404) USA300HOU_2370 → / → USA300HOU_2372 hypothetical protein/MerR family transcriptional regulator
RA 2,532,298 A→T 100% T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,566,074 C→T 9.5% G20R (GGA→AGA)  USA300HOU_2426 ← hypothetical protein
RA 2,765,350 T→C 14.3% intergenic (‑419/+276) gpxA2 ← / ← USA300HOU_2620 glutathione peroxidase/hypothetical protein
RA 2,847,358 C→T 16.7% N61N (AAC→AAT USA300HOU_2689 → hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 211704 =12 (0.550)5 (0.250) 3/242 NT 28.4% intergenic (+262/+66) USA300HOU_0193/USA300HOU_0194 possible holo‑[acyl‑carrier‑protein] synthase/hypothetical membrane protein
?CP000730 211755 = 14 (0.690)intergenic (+313/+15) USA300HOU_0193/USA300HOU_0194 possible holo‑[acyl‑carrier‑protein] synthase/hypothetical membrane protein
* ? CP000730 = 187342323 (1.060)5 (0.260) 3/234 NT 19.0% intergenic (‑99/+651) USA300HOU_1742/USA300HOU_1743 M20 family peptidase/hypothetical protein
?CP000730 = 1873461 22 (1.120)intergenic (‑137/+613) USA300HOU_1742/USA300HOU_1743 M20 family peptidase/hypothetical protein