breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 39,378 C→A D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 367,644 G→T intergenic (‑96/‑20) nanA ← / → nagC N‑acetylneuraminate lyase/possible transcriptional regulator
RA 423,247 A→G V112V (GTA→GTG USA300HOU_0394 → hypothetical protein
RA 454,656 G→A V119I (GTT→ATT)  set23 → staphylococcal exotoxin
RA 479,553 G→A A194T (GCA→ACA)  USA300HOU_0458 → hypothetical protein
RA 638,614 G→T T14K (ACA→AAA)  thiD ← phosphomethylpyrimidine kinase
RA 707,879 A→T L11I (TTA→ATA)  mntB ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 790,778 G→A intergenic (+277/‑197) kipA → / → ltaS possible allophanate hydrolase subunit 2/possible phosphatidylglycerol‑‑membrane‑oligosaccharide glycerophosphotransferase
RA 911,610 G→A intergenic (‑221/‑107) USA300HOU_0869 ← / → sufC hypothetical membrane protein/ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,232,465 G→C A124P (GCG→CCG)  prkC → non‑specific serine/threonine protein kinase
MC JC 1,302,967 Δ4 bp coding (325‑328/714 nt) USA300HOU_1209 → possible GntR family transcriptional regulator
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,419,527 G→T P7T (CCT→ACT)  USA300HOU_1317 ← hypothetical membrane protein
RA 1,440,007 Δ1 bp coding (34/201 nt) cspA ← cold shock protein CspA
RA 1,474,639 C→G A9129P (GCA→CCA)  ebh ← extracellular matrix binding protein
RA 1,481,355 G→T A6890E (GCA→GAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,672,434 C→T A187T (GCA→ACA)  rpoD ← DNA‑directed RNA polymerase sigma subunit RpoD
RA 1,744,454 C→T G73D (GGC→GAC)  apt ← adenine phosphoribosyltransferase
RA 1,797,772 G→C R7G (CGC→GGC)  phoR ← sensor histidine kinase
RA 1,944,670 G→A intergenic (‑251/‑713) splA ← / → USA300HOU_1807 S1B family serine protease SplA/hypothetical protein
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,026,655 C→T E87K (GAA→AAA)  vraR ← response regulator VraR
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,325 C→A Q55H (CAG→CAT ybbP ← hypothetical membrane protein
RA 2,386,119 G→A P114L (CCG→CTG)  USA300HOU_2245 ← hypothetical protein
RA 2,502,141 C→A A222D (GCC→GAC)  USA300HOU_2364 → AraC transcriptional regulator
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,679,462 (A)8→7 intergenic (+334/‑55) USA300HOU_2535 → / → cbpD hypothetical protein/possible secretory antigen
RA 2,710,898 (T)6→5 coding (29/1812 nt) oatA ← possible acetyltransferase
RA 2,856,087 T→A P80P (CCT→CCA USA300HOU_2698 → hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =38 (1.110)32 (1.310)
+40 bp
26/198 0.1 54.2% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA