breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 15,571 T→C 100% L43L (TTG→CTG)  EK12AG100_RS00080 → IS4‑like element IS421 family transposase
RA 210,842 C→T 10.3% A332A (GCC→GCT ldcC → lysine decarboxylase LdcC
RA 283,927 G→T 8.8% A445A (GCG→GCT EK12AG100_RS01390 → xylonate dehydratase YagF
RA 312,935 A→G 6.0% intergenic (‑301/+173) EK12AG100_RS01540 ← / ← EK12AG100_RS24935 hypothetical protein/oxidoreductase
RA 384,896 A→C 100% G122G (GGA→GGC tauA → taurine ABC transporter substrate‑binding protein
RA 472,148 A→G 100% G84G (GGA→GGG glnK → P‑II family nitrogen regulator
RA 575,023 C→T 21.6% G10G (GGG→GGA EK12AG100_RS02845 ← IS5‑like element IS5 family transposase
RA 575,273 G→T 90.9% R2L (CGA→CTA)  rzoD → prophage lysis lipoprotein RzoD
RA 575,369 C→G 9.4% P34R (CCG→CGG)  rzoD → prophage lysis lipoprotein RzoD
RA 575,513 T→C 5.9% intergenic (+62/+29) rzoD → / ← bor prophage lysis lipoprotein RzoD/serum resistance lipoprotein Bor
RA 575,516 C→A 5.4% intergenic (+65/+26) rzoD → / ← bor prophage lysis lipoprotein RzoD/serum resistance lipoprotein Bor
RA 575,547 G→T 5.2% Q97K (CAA→AAA)  bor ← serum resistance lipoprotein Bor
RA 576,068 C→T 5.8% intergenic (‑233/+57) bor ← / ← EK12AG100_RS02865 serum resistance lipoprotein Bor/DUF1398 domain‑containing protein
RA 576,212 A→G 47.7% H108H (CAT→CAC EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,226 C→T 48.9% V104I (GTT→ATT)  EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,326 G→A 46.6% N70N (AAC→AAT EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,398 G→A 47.9% I46I (ATC→ATT EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,443 G→A 48.4% N31N (AAC→AAT EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,481 C→T 46.8% D19N (GAC→AAC)  EK12AG100_RS02865 ← DUF1398 domain‑containing protein
RA 576,592 T→C 50.1% intergenic (‑57/‑229) EK12AG100_RS02865 ← / → EK12AG100_RS02870 DUF1398 domain‑containing protein/hypothetical protein
RA 576,612 T→C 47.8% intergenic (‑77/‑209) EK12AG100_RS02865 ← / → EK12AG100_RS02870 DUF1398 domain‑containing protein/hypothetical protein
RA 576,658 C→A 46.9% intergenic (‑123/‑163) EK12AG100_RS02865 ← / → EK12AG100_RS02870 DUF1398 domain‑containing protein/hypothetical protein
RA 576,752 T→C 45.6% intergenic (‑217/‑69) EK12AG100_RS02865 ← / → EK12AG100_RS02870 DUF1398 domain‑containing protein/hypothetical protein
RA 576,961 A→G 47.7% R47R (AGA→AGG EK12AG100_RS02870 → hypothetical protein
RA 577,056 T→C 49.6% intergenic (+29/+136) EK12AG100_RS02870 → / ← EK12AG100_RS02875 hypothetical protein/YlcI/YnfO family protein
RA 577,060 A→G 48.8% intergenic (+33/+132) EK12AG100_RS02870 → / ← EK12AG100_RS02875 hypothetical protein/YlcI/YnfO family protein
RA 577,061 C→T 49.9% intergenic (+34/+131) EK12AG100_RS02870 → / ← EK12AG100_RS02875 hypothetical protein/YlcI/YnfO family protein
RA 577,243 T→C 55.3% R48R (AGA→AGG EK12AG100_RS02875 ← YlcI/YnfO family protein
RA 577,378 A→C 28.4% T3T (ACT→ACG EK12AG100_RS02875 ← YlcI/YnfO family protein
RA 577,658 A→G 93.9% intergenic (‑272/‑117) EK12AG100_RS02875 ← / → EK12AG100_RS02885 YlcI/YnfO family protein/DNA‑packaging protein
RA 577,687 C→T 13.2% intergenic (‑301/‑88) EK12AG100_RS02875 ← / → EK12AG100_RS02885 YlcI/YnfO family protein/DNA‑packaging protein
RA 577,771 T→A 32.5% intergenic (‑385/‑4) EK12AG100_RS02875 ← / → EK12AG100_RS02885 YlcI/YnfO family protein/DNA‑packaging protein
RA 577,772 G→A 32.8% intergenic (‑386/‑3) EK12AG100_RS02875 ← / → EK12AG100_RS02885 YlcI/YnfO family protein/DNA‑packaging protein
RA 577,983 G→T 37.9% R70L (CGG→CTG)  EK12AG100_RS02885 → DNA‑packaging protein
RA 577,997 G→A 36.3% A75T (GCA→ACA)  EK12AG100_RS02885 → DNA‑packaging protein
RA 578,014 G→A 65.4% G80G (GGG→GGA EK12AG100_RS02885 → DNA‑packaging protein
RA 578,059 C→T 30.6% D95D (GAC→GAT EK12AG100_RS02885 → DNA‑packaging protein
RA 578,068 A→G 29.3% E98E (GAA→GAG EK12AG100_RS02885 → DNA‑packaging protein
RA 578,074 A→G 71.5% K100K (AAA→AAG EK12AG100_RS02885 → DNA‑packaging protein
RA 578,126 T→C 27.4% L118L (TTG→CTG)  EK12AG100_RS02885 → DNA‑packaging protein
RA 578,168 C→A 30.7% L132I (CTC→ATC)  EK12AG100_RS02885 → DNA‑packaging protein
RA 579,538 T→C 6.1% N61S (AAT→AGT)  EK12AG100_RS02895 ← class I SAM‑dependent methyltransferase
RA 718,545 A→G 5.1% C44R (TGT→CGT)  kdpE ← two‑component system response regulator KdpE
JC 932,107 Δ168 bp 100% [EK12AG100_RS04715] [EK12AG100_RS04715]
RA 1,015,338 A→C 100% L202V (TTA→GTA)  elyC ← envelope biogenesis factor ElyC
RA 1,123,847 G→A 100% intergenic (+494/‑49) putP → / → EK12AG100_RS05615 sodium/proline symporter PutP/FTR1 family protein
RA 1,154,267 T→C 5.9% L289L (TTG→CTG) ‡ mdoH → glucans biosynthesis glucosyltransferase MdoH
RA 1,154,268 T→C 5.8% L289S (TTG→TCG) ‡ mdoH → glucans biosynthesis glucosyltransferase MdoH
RA 1,232,520 G→A 5.1% T156T (ACC→ACT phoP ← two‑component system response regulator PhoP
MC JC 1,238,818 Δ84 bp 100% coding (1156‑1239/1335 nt) EK12AG100_RS06230 → NADP‑dependent isocitrate dehydrogenase
RA 1,332,957 G→T 5.9% A325S (GCC→TCC)  oppF → murein tripeptide/oligopeptide ABC transporter ATP binding protein OppF
RA 1,363,615 A→G 93.3% S522G (AGC→GGC)  acnA → aconitate hydratase AcnA
RA 1,518,134 A→C 5.1% E217A (GAG→GCG)  EK12AG100_RS07620 → ABC transporter permease
RA 1,637,283 A→G 7.0% Q3R (CAA→CGA)  EK12AG100_RS08155 → recombinase family protein
RA 1,639,728 T→C 100% E98E (GAA→GAG EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,639,737 G→A 30.6% D95D (GAC→GAT EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,639,799 C→T 100% A75T (GCA→ACA)  EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,639,813 C→A 19.8% R70L (CGG→CTG)  EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,639,827 T→C 18.2% E65E (GAA→GAG EK12AG100_RS08170 ← DNA‑packaging protein
RA 1,640,036 A→C 60.3% intergenic (‑15/‑374) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,067 C→A 43.9% intergenic (‑46/‑343) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,077 T→C 44.8% intergenic (‑56/‑333) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,109 G→A 48.5% intergenic (‑88/‑301) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,138 T→C 49.4% intergenic (‑117/‑272) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
RA 1,640,157 C→T 48.0% intergenic (‑136/‑253) EK12AG100_RS08170 ← / → EK12AG100_RS08175 DNA‑packaging protein/YlcI/YnfO family protein
JC 1,870,601 Δ182 bp 85.7% [EK12AG100_RS09385] [EK12AG100_RS09385]
RA 2,484,484 G→A 7.3% S190L (TCA→TTA)  emrY ← multidrug efflux MFS transporter permease subunit EmrY
MC JC 2,561,645 Δ90 bp 100% [eutA] [eutA]
JC 2,694,580 Δ128 bp 100% [EK12AG100_RS25720] [EK12AG100_RS25720]
RA 2,783,895 G→A 5.1% intergenic (+157/‑51) EK12AG100_RS25740 → / → EK12AG100_RS25255 alpha amylase/alpha amylase
RA 3,067,161 T→G 100% K129N (AAA→AAC fbaA ← class II fructose‑bisphosphate aldolase
RA 3,551,559 T→G 5.4% C655G (TGC→GGC)  malT → HTH‑type transcriptional regulator MalT
RA 3,556,874 A→G 5.8% V82A (GTC→GCC)  EK12AG100_RS17745 ← DeoR/GlpR family transcriptional regulator
RA 3,643,941 T→C 8.1% V377A (GTG→GCG)  gorA → glutathione‑disulfide reductase
RA 3,717,968 T→C 8.7% L259L (CTT→CTC EK12AG100_RS25320 → IS3‑like element IS150 family transposase
RA 3,862,813 G→A 6.0% intergenic (‑28/+168) EK12AG100_RS19170 ← / ← ibpB putative transporter/heat shock chaperone IbpB
RA 3,862,823 A→G 5.5% intergenic (‑38/+158) EK12AG100_RS19170 ← / ← ibpB putative transporter/heat shock chaperone IbpB
RA 3,872,045 T→G 15.8% H32Q (CAT→CAG EK12AG100_RS19225 → hypothetical protein
RA 3,940,810 C→T 100% noncoding (604/2886 nt) EK12AG100_RS19545 → 23S ribosomal RNA
RA 4,022,771 T→G 100% Y437* (TAT→TAG ubiD → 4‑hydroxy‑3‑polyprenylbenzoate decarboxylase
RA 4,023,909 T→G 74.6% intergenic (+208/+173) EK12AG100_RS19960 → / ← fadA NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA
RA 4,023,921 G→A 64.7% intergenic (+220/+161) EK12AG100_RS19960 → / ← fadA NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA
RA 4,023,940 A→T 53.3% intergenic (+239/+142) EK12AG100_RS19960 → / ← fadA NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA
RA 4,023,942 C→G 53.3% intergenic (+241/+140) EK12AG100_RS19960 → / ← fadA NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA
RA 4,023,947 G→A 47.1% intergenic (+246/+135) EK12AG100_RS19960 → / ← fadA NAD(P)H‑flavin reductase/acetyl‑CoA C‑acyltransferase FadA
RA 4,033,969 A→T 47.0% intergenic (+161/‑14) EK12AG100_RS20005 → / → EK12AG100_RS20010 tRNA‑Ala/23S ribosomal RNA
RA 4,220,111 T→C 100% intergenic (‑10/‑190) iclR ← / → metH glyoxylate bypass operon transcriptional repressor IclR/methionine synthase
RA 4,264,972 T→A 100% intergenic (+192/+310) EK12AG100_RS21080 → / ← EK12AG100_RS25365 aromatic amino acid transaminase/hypothetical protein
RA 4,292,533 T→C 100% intergenic (+266/+376) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,663 A→G 16.0% intergenic (+396/+246) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,664 A→C 16.0% intergenic (+397/+245) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,741 C→T 75.2% intergenic (+474/+168) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,759 C→T 14.6% intergenic (+492/+150) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,292,853:1 +CG 100% intergenic (+586/+56) gltP → / ← EK12AG100_RS21205 glutamate/aspartate:proton symporter GltP/sel1 repeat family protein
RA 4,470,374 T→C 5.7% R52G (AGA→GGA)  bdcA ← SDR family oxidoreductase
RA 4,543,075 T→C 8.9% I502I (ATT→ATC fimD → fimbrial biogenesis usher protein FimD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_LN832404 575054 575260 207 140 [0] [41] 43 EK12AG100_RS02845/rzoD IS5‑like element IS5 family transposase/prophage lysis lipoprotein RzoD
* * ÷ NZ_LN832404 822786 822992 207 44 [42] [2] 119 [rzoD]–[EK12AG100_RS04195] [rzoD],[EK12AG100_RS04195]
* * ÷ NZ_LN832404 1982331 1982362 32 108 [0] [0] 136 flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* * ÷ NZ_LN832404 3126612–3127597 3127900 304–1289 43 [42] [0] 418 [EK12AG100_RS15510] [EK12AG100_RS15510]
* * ÷ NZ_LN832404 3420340–3420992 3420992 1–653 43 [42] [42] 43 [EK12AG100_RS17000] [EK12AG100_RS17000]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_LN832404 = 614890 (0.000)66 (0.920) 59/410 NT 100% intergenic (‑76/+87) EK12AG100_RS23030/rapA RNA polymerase‑associated protein RapA/RNA polymerase‑associated protein RapA
?NZ_LN832404 = 381811 NA (NA)coding (847/914 nt) EK12AG100_RS01865 IS3‑like element IS2 family transposase
* ? NZ_LN832404 61489 =0 (0.000)56 (0.820) 51/394 NT 100% intergenic (‑76/+87) EK12AG100_RS23030/rapA RNA polymerase‑associated protein RapA/RNA polymerase‑associated protein RapA
?NZ_LN832404 380645 = NA (NA)coding (41/361 nt) EK12AG100_RS01865 IS3‑like element IS2 family transposase
* ? NZ_LN832404 = 274541NA (NA)112 (1.130) 93/566 NT 45.2% intergenic (‑32/‑176) EK12AG100_RS01335/mmuP IS5‑like element IS5 family transposase/S‑methylmethionine permease
?NZ_LN832404 576125 = 136 (1.380)coding (411/411 nt) EK12AG100_RS02865 DUF1398 domain‑containing protein
* ? NZ_LN832404 = 381810NA (NA)77 (1.080) 71/408 NT 100% coding (846/914 nt) EK12AG100_RS01865 IS3‑like element IS2 family transposase
?NZ_LN832404 = 932184 0 (0.000)intergenic (‑74/‑445) EK12AG100_RS04715/EK12AG100_RS04720 aspartate:alanine antiporter/hypothetical protein
* ? NZ_LN832404 = 5750530 (0.000)106 (1.170) 23/520 NT 100% intergenic (‑1/‑216) EK12AG100_RS02845/rzoD IS5‑like element IS5 family transposase/prophage lysis lipoprotein RzoD
?NZ_LN832404 1982363 = 0 (0.000)intergenic (‑330/‑326) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_LN832404 = 578602107 (1.080)83 (1.230) 76/388 NT 69.4% coding (308/744 nt) EK12AG100_RS02890 phage terminase large subunit family protein
?NZ_LN832404 822993 = 0 (0.000)coding (129/1746 nt) EK12AG100_RS04195 phage terminase large subunit family protein
* ? NZ_LN832404 804269 =92 (0.930)11 (0.120) 11/536 NT 10.2% intergenic (‑47/+88) EK12AG100_RS03990/EK12AG100_RS03995 putative acyl‑CoA thioester hydrolase/tyrosine‑type recombinase/integrase
?NZ_LN832404 = 849699 107 (1.150)intergenic (‑750/+106) EK12AG100_RS04330/EK12AG100_RS04335 AAA family ATPase/kinase inhibitor
* ? NZ_LN832404 932185 =0 (0.000)55 (0.790) 50/398 NT 100% intergenic (‑75/‑444) EK12AG100_RS04715/EK12AG100_RS04720 aspartate:alanine antiporter/hypothetical protein
?NZ_LN832404 = 1472810 NA (NA)coding (39/361 nt) EK12AG100_RS07405 IS3‑like element IS2 family transposase
* ? NZ_LN832404 = 1423459NA (NA)101 (1.020) 7/566 NT 100% intergenic (+146/‑127) EK12AG100_RS07185/EK12AG100_RS07190 IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_LN832404 = 1982330 0 (0.000)intergenic (‑297/‑359) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_LN832404 = 1423461NA (NA)87 (0.970)
+27 bp
70/512 NT 100% intergenic (+148/‑125) EK12AG100_RS07185/EK12AG100_RS07190 IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_LN832404 = 2242224 0 (0.000)intergenic (‑1/+2) mglA/mglB galactose/methyl galactoside ABC transporter ATP‑binding protein MglA/galactose/glucose ABC transporter substrate‑binding protein MglB
* ? NZ_LN832404 = 1472808NA (NA)65 (0.950) 56/394 NT 100% coding (41/361 nt) EK12AG100_RS07405 IS3‑like element IS2 family transposase
?NZ_LN832404 = 1870685 0 (0.000)intergenic (‑559/‑65) EK12AG100_RS09380/EK12AG100_RS09385 scaffolding protein MipA/protein kinase YeaG
* ? NZ_LN832404 = 1474132NA (NA)115 (1.350) 102/490 NT 100% coding (1121/1152 nt) EK12AG100_RS07415 IS30‑like element IS30 family transposase
?NZ_LN832404 2694668 = 0 (0.000)intergenic (‑89/+37) yfhb/EK12AG100_RS25720 phosphatidylglycerophosphatase C/phosphatidylglycerophosphatase C
* ? NZ_LN832404 = 1655111NA (NA)54 (0.810) 51/384 NT 100% pseudogene (616/696 nt) EK12AG100_RS08315 IS3‑like element IS2 family transposase
?NZ_LN832404 1870686 = 0 (0.000)intergenic (‑560/‑64) EK12AG100_RS09380/EK12AG100_RS09385 scaffolding protein MipA/protein kinase YeaG
* ? NZ_LN832404 = 2105880NA (NA)81 (0.930)
+32 bp
70/502 NT 100% intergenic (‑33/+2) EK12AG100_RS10660/EK12AG100_RS10665 IS5‑like element IS5 family transposase/glycosyltransferase
?NZ_LN832404 2242225 = 0 (0.000)intergenic (‑2/+1) mglA/mglB galactose/methyl galactoside ABC transporter ATP‑binding protein MglA/galactose/glucose ABC transporter substrate‑binding protein MglB
* ? NZ_LN832404 = 26946690 (0.000)60 (0.890) 53/386 NT 100% intergenic (‑90/+36) yfhb/EK12AG100_RS25720 phosphatidylglycerophosphatase C/phosphatidylglycerophosphatase C
?NZ_LN832404 = 4505063 NA (NA)coding (29/1152 nt) EK12AG100_RS22270 IS30‑like element IS30 family transposase
* ? NZ_LN832404 = 3127562NA (NA)76 (1.080) 68/404 NT 100% intergenic (+65/+418) EK12AG100_RS15510/EK12AG100_RS15515 IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein
?NZ_LN832404 3127901 = 0 (0.000)intergenic (+404/+79) EK12AG100_RS15510/EK12AG100_RS15515 IS5‑like element IS5 family transposase/lipopolysaccharide biosynthesis protein
* ? NZ_LN832404 = 3935137108 (1.090)6 (0.060) 6/566 NT 5.3% coding (399/993 nt) rbsR ribose operon transcriptional repressor RbsR
?NZ_LN832404 4494655 = NA (NA)intergenic (+241/‑46) EK12AG100_RS22200/EK12AG100_RS22210 DUF4102 domain‑containing protein/IS3‑like element IS2 family transposase
* ? NZ_LN832404 4539156 =22 (0.220)81 (0.850) 72/548 NT 80.9% intergenic (+49/‑433) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NZ_LN832404 4539470 = 17 (0.180)intergenic (+363/‑119) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
* ? NZ_LN832404 = 453916422 (0.220)97 (1.020) 89/548 NT 83.5% intergenic (+57/‑425) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA
?NZ_LN832404 = 4539460 17 (0.180)intergenic (+353/‑129) fimE/fimA type 1 fimbria regulatory protein FimE/metal ABC transporter substrate‑binding lipoprotein/fibrin‑binding adhesin FimA