breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 25 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,171,8570AT25.1% 308.4 / 124.5 175intergenic (+24/+22)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
*NC_0009133,171,8630AG25.1% 282.9 / 112.2 180intergenic (+30/+16)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
*NC_0009133,946,2460GA25.0% 277.4 / 90.4 160noncoding (2543/2904 nt)rrlC23S ribosomal RNA of rrnC operon
*NC_0009133,171,8580AT24.7% 318.1 / 124.4 175intergenic (+25/+21)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
*NC_0009133,171,8520CT24.3% 318.8 / 103.7 181intergenic (+19/+27)qseC/ygiZquorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
*NC_0009134,520,5041.T24.2% 398.7 / 179.6 161intergenic (‑180/+167)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
*NC_0009134,296,0600CT24.0% 531.8 / 161.7 294intergenic (+266/+376)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009131,674,9580AT23.9% 321.3 / 104.3 163intergenic (+11/+14)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
*NC_0009131,674,9550AT23.3% 310.1 / 101.7 163intergenic (+8/+17)tqsA/pntBpheromone AI‑2 transporter/pyridine nucleotide transhydrogenase, beta subunit
*NC_0009134,520,5000A.23.3% 427.2 / 138.0 166intergenic (‑176/+171)yjhU/yjhFputative DNA‑binding transcriptional regulator; KpLE2 phage‑like element/putative transporter
*NC_0009131,764,6870TC23.0% 282.1 / 86.8 162intergenic (‑301/+26)sufA/rydBFe‑S cluster assembly protein/novel sRNA, function unknown
*NC_0009131,764,7010GA21.9% 298.2 / 82.2 160intergenic (‑315/+12)sufA/rydBFe‑S cluster assembly protein/novel sRNA, function unknown
*NC_0009133,407,2930TA21.4% 368.2 / 95.9 173intergenic (+27/‑82)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
*NC_0009133,181,2980AT21.0% 329.6 / 83.4 157H293L (CAT→CTT) ygiCATP‑Grasp family ATPase
*NC_0009133,407,2950TA20.9% 371.9 / 95.2 173intergenic (+29/‑80)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
*NC_0009132,081,6880AT20.9% 327.6 / 88.2 158I287N (ATT→AAT) dacDD‑alanyl‑D‑alanine carboxypeptidase; penicillin‑binding protein 6b
*NC_0009132,032,3360GC20.9% 367.8 / 91.6 163intergenic (‑19/+48)dcm/yedJDNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
*NC_0009132,142,5050GC20.8% 380.3 / 98.2 168R148R (CGC→CGGudkuridine/cytidine kinase
*NC_0009133,407,2940TA20.8% 379.7 / 94.9 173intergenic (+28/‑81)accC/yhdTacetyl‑CoA carboxylase, biotin carboxylase subunit/DUF997 family putative inner membrane protein
*NC_0009134,472,4370TC20.8% 295.4 / 76.0 159N33D (AAT→GAT) pyrLpyrBI operon leader peptide

Marginal new junction evidence (lowest skew 10 of 1290 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1468681NA (NA)111 (1.350)
+44 bp
12/108 9.2 NA noncoding (560/1331 nt) IS2 repeat region
?NC_000913 1650843 = NA (NA)noncoding (1/706 nt) IS2 repeat region
* ? NC_000913 = 417160733 (0.220)112 (0.800) 21/184 11.9 78.3% intergenic (+63/‑30) rrlB/rrfB 23S ribosomal RNA of rrnB operon/5S ribosomal RNA of rrnB operon
?NC_000913 = 4213022 NA (NA)intergenic (+76/‑18) rrlE/rrfE 23S ribosomal RNA of rrnE operon/5S ribosomal RNA of rrnE operon
* ? NC_000913 = 3946688149 (1.000)111 (0.790) 21/184 11.9 44.2% intergenic (+81/‑12) rrlC/rrfC 23S ribosomal RNA of rrnC operon/5S ribosomal RNA of rrnC operon
?NC_000913 = 4171601 NA (NA)intergenic (+57/‑36) rrlB/rrfB 23S ribosomal RNA of rrnB operon/5S ribosomal RNA of rrnB operon
* ? NC_000913 2726200 =NA (NA)111 (0.790) 21/184 11.9 NA intergenic (‑12/+81) rrfG/rrlG 5S ribosomal RNA of rrnG operon/23S ribosomal RNA of rrnG operon
?NC_000913 2726224 = NA (NA)intergenic (‑36/+57) rrfG/rrlG 5S ribosomal RNA of rrnG operon/23S ribosomal RNA of rrnG operon
* ? NC_000913 = 22874458 (0.390)111 (0.790) 21/184 11.9 67.1% intergenic (+82/‑12) rrlH/rrfH 23S ribosomal RNA of rrnH operon/5S ribosomal RNA of rrnH operon
?NC_000913 2726224 = NA (NA)intergenic (‑36/+57) rrfG/rrlG 5S ribosomal RNA of rrnG operon/23S ribosomal RNA of rrnG operon
* ? NC_000913 = 2304902NA (NA)36 (0.360)
+33 bp
11/130 12.2 NA noncoding (486/655 nt) REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences
?NC_000913 = 2305065 NA (NA)noncoding (649/655 nt) REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences REP161 (repetitive extragenic palindromic) element; contains 12 REP sequences
* ? NC_000913 225078 =NA (NA)76 (0.550) 17/182 13.6 NA noncoding (1308/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 225100 = NA (NA)noncoding (1330/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 = 20668320 (0.000)52 (0.380) 16/178 13.8 100% noncoding (522/1195 nt) IS5 repeat region
?NC_000913 = 2066857 NA (NA)noncoding (497/1195 nt) IS5 repeat region
* ? NC_000913 3941442 =153 (1.020)24 (0.280)
+41 bp
6/114 13.9 21.3% intergenic (‑115/‑366) yieP/rrsC putative transcriptional regulator/16S ribosomal RNA of rrnC operon
?NC_000913 4035099 = 152 (1.020)coding (492/546 nt) hemG protoporphyrin oxidase, flavoprotein
* ? NC_000913 = 225281NA (NA)76 (0.540) 17/186 13.9 NA noncoding (1511/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 = 225293 NA (NA)noncoding (1523/1542 nt) rrsH 16S ribosomal RNA of rrnH operon