breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 81 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_021214358,5110GA35.8% 249.7 / 71.7 165intergenic (‑93/‑92)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_0212141,239,6280TG32.2% 363.6 / 53.4 216C97G (TGT→GGT) TMARI_RS06210oxidoreductase
*NC_021214825,7450CT30.7% 318.9 / 71.9 193P81S (CCC→TCC) TMARI_RS04110histidinol‑phosphatase
*NC_021214192,9140TG30.0% 315.0 / 17.5 191noncoding (2147/3022 nt)TMARI_RS0089023S ribosomal RNA
*NC_021214769,6610AG29.9% 207.0 / 106.1 167S296P (TCG→CCG) TMARI_RS03820S41 family peptidase
*NC_021214232,2000GA29.5% 413.0 / 82.7 218R259Q (CGA→CAA) TMARI_RS01120glycine‑‑tRNA ligase subunit beta
*NC_021214358,5240TA29.4% 374.5 / 48.4 172intergenic (‑106/‑79)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_021214724,1700AT27.6% 476.7 / 78.8 214I257K (ATA→AAA) TMARI_RS03585chemotaxis protein CheA
*NC_021214769,6570GT27.1% 254.2 / 74.5 171A297D (GCC→GAC) ‡TMARI_RS03820S41 family peptidase
*NC_021214769,6550AT27.0% 281.2 / 92.1 176S298T (TCG→ACG) TMARI_RS03820S41 family peptidase
*NC_021214480,4430TG26.9% 442.3 / 54.3 247G682G (GGT→GGGTMARI_RS02340DNA‑directed RNA polymerase subunit beta
*NC_021214769,6560GC26.6% 317.0 / 104.7 171A297A (GCC→GCG) ‡TMARI_RS03820S41 family peptidase
*NC_021214769,6580CT26.6% 241.2 / 75.4 170A297T (GCC→ACC) ‡TMARI_RS03820S41 family peptidase
*NC_0212141,001,0510AC26.6% 227.0 / 25.0 109I388I (ATA→ATCTMARI_RS05040DUF1156 domain‑containing protein
*NC_0212141,777,0550AT26.6% 360.4 / 47.8 160N62K (AAT→AAATMARI_RS09225type I‑B CRISPR‑associated protein Cas5
*NC_021214769,6640CT26.5% 254.4 / 71.5 167G295R (GGA→AGA) TMARI_RS03820S41 family peptidase
*NC_0212141,869,4240CG26.1% 150.0 / 23.6 172intergenic (+36/‑75)TMARI_RS09635/TMARI_RS09660bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/hypothetical protein
*NC_021214769,6630CT25.8% 246.3 / 62.3 167G295E (GGA→GAA) TMARI_RS03820S41 family peptidase
*NC_021214825,7530TG25.1% 377.8 / 23.8 180G83G (GGT→GGGTMARI_RS04110histidinol‑phosphatase
*NC_021214769,6600GT25.0% 316.6 / 64.9 168S296* (TCG→TAG) TMARI_RS03820S41 family peptidase

Marginal new junction evidence (lowest skew 10 of 39 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_021214 = 141342453 (1.310)45 (0.130) 22/448 16.1 9.0% coding (409/1233 nt) TMARI_RS00650 transposase
?NC_021214 141347 = 454 (1.320)coding (404/1233 nt) TMARI_RS00650 transposase
* ? NC_021214 = 18291603658 (10.570)12 (0.040) 12/434 20.3 0.34% coding (524/1485 nt) TMARI_RS09450 sugar ABC transporter ATP‑binding protein
?NC_021214 = 1829167 3490 (10.460)coding (517/1485 nt) TMARI_RS09450 sugar ABC transporter ATP‑binding protein
* ? NC_021214 1327950 =348 (1.010)12 (0.040) 6/430 24.1 3.5% coding (408/762 nt) TMARI_RS06635 ABC transporter permease
?NC_021214 1328134 = 335 (1.010)coding (592/762 nt) TMARI_RS06635 ABC transporter permease
* ? NC_021214 814725 =306 (0.880)12 (0.040) 6/440 24.4 3.6% coding (243/639 nt) TMARI_RS04050 transporter
?NC_021214 814876 = 335 (0.990)coding (394/639 nt) TMARI_RS04050 transporter
* ? NC_021214 = 560268313 (0.900)10 (0.030) 5/432 25.0 3.3% coding (172/618 nt) TMARI_RS02740 hypothetical protein
?NC_021214 = 560304 277 (0.830)coding (208/618 nt) TMARI_RS02740 hypothetical protein
* ? NC_021214 454940 =323 (0.930)6 (0.020) 5/434 25.0 1.8% coding (1064/1104 nt) TMARI_RS02195 pectate trisaccharide‑lyase
?NC_021214 1085145 = 326 (0.980)coding (728/759 nt) TMARI_RS05450 DeoR/GlpR transcriptional regulator
* ? NC_021214 = 1335343369 (1.070)4 (0.010) 4/410 25.1 1.2% intergenic (+50/‑27) TMARI_RS06690/TMARI_RS06695 hypothetical protein/Cys‑rich RiPP peptide
?NC_021214 = 1335323 342 (1.090)intergenic (+30/‑47) TMARI_RS06690/TMARI_RS06695 hypothetical protein/Cys‑rich RiPP peptide
* ? NC_021214 1824914 =4107 (11.870)16 (0.050) 5/440 25.2 0.42% coding (2255/2532 nt) TMARI_RS09435 pullulanase
?NC_021214 1825106 = 3543 (10.480)coding (2447/2532 nt) TMARI_RS09435 pullulanase
* ? NC_021214 1020436 =345 (1.000)6 (0.020) 5/438 25.2 1.8% coding (16/555 nt) TMARI_RS05115 diguanylate cyclase
?NC_021214 = 1020432 336 (1.000)coding (20/555 nt) TMARI_RS05115 diguanylate cyclase
* ? NC_021214 = 22265285 (0.820)6 (0.020) 5/440 25.2 2.1% coding (1123/1929 nt) TMARI_RS00100 laminarinase
?NC_021214 = 22484 289 (0.850)coding (904/1929 nt) TMARI_RS00100 laminarinase