breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 50 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_021214449,0560TC47.4% ‑5.2 / 21.6 19G501G (GGA→GGGTMARI_RS02175methyl‑accepting chemotaxis protein 1
*NC_021214358,5110GA33.5% 232.5 / 65.7 159intergenic (‑93/‑92)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_021214769,6610AG31.8% 217.7 / 96.0 173S296P (TCG→CCG) TMARI_RS03820S41 family peptidase
*NC_021214358,5240TA31.2% 319.0 / 47.0 158intergenic (‑106/‑79)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_021214232,2000GA29.2% 482.9 / 108.5 279R259Q (CGA→CAA) TMARI_RS01120glycine‑‑tRNA ligase subunit beta
*NC_021214480,4430TG28.1% 445.7 / 74.7 298G682G (GGT→GGGTMARI_RS02340DNA‑directed RNA polymerase subunit beta
*NC_021214769,6570GT28.0% 225.0 / 60.3 169A297D (GCC→GAC) ‡TMARI_RS03820S41 family peptidase
*NC_021214769,6640CT27.1% 246.9 / 64.2 174G295R (GGA→AGA) TMARI_RS03820S41 family peptidase
*NC_021214769,6560GC26.8% 309.3 / 75.0 169A297A (GCC→GCG) ‡TMARI_RS03820S41 family peptidase
*NC_021214358,5150CG26.6% 352.9 / 34.6 158intergenic (‑97/‑88)TMARI_RS01730/TMARI_RS01735hypothetical protein/DUF2089 domain‑containing protein
*NC_021214693,6230GT26.5% 344.3 / 94.4 212A237A (GCC→GCATMARI_RS03400cysteine synthase A
*NC_021214769,6620TC26.2% 277.7 / 55.5 174G295G (GGA→GGGTMARI_RS03820S41 family peptidase
*NC_0212141,239,6280TG26.1% 379.0 / 40.6 229C97G (TGT→GGT) TMARI_RS06210oxidoreductase
*NC_021214401,6910GT25.6% 449.6 / 47.7 180L130L (CTC→CTATMARI_RS01945endonuclease III domain‑containing protein
*NC_021214769,6580CT25.3% 243.3 / 57.8 170A297T (GCC→ACC) ‡TMARI_RS03820S41 family peptidase
*NC_021214693,6430TA25.0% 401.3 / 72.9 208T231S (ACC→TCC) TMARI_RS03400cysteine synthase A
*NC_0212141,869,4340TC25.0% 402.6 / 19.0 196intergenic (+46/‑65)TMARI_RS09635/TMARI_RS09660bifunctional UDP‑sugar hydrolase/5'‑nucleotidase/hypothetical protein
*NC_021214693,6370CT24.9% 311.8 / 68.9 204E233K (GAG→AAG) TMARI_RS03400cysteine synthase A
*NC_021214656,6130AC24.3% 434.1 / 45.5 222G234G (GGT→GGGTMARI_RS03190glycosyl transferase family 1
*NC_0212141,777,0550AT24.0% 379.6 / 35.9 181N62K (AAT→AAATMARI_RS09225type I‑B CRISPR‑associated protein Cas5

Marginal new junction evidence (lowest skew 10 of 43 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_021214 = 1021271440 (1.370)48 (0.150)
+ACAA
17/448 14.6 10.0% coding (406/441 nt) TMARI_RS05125 transposase
?NC_021214 1021272 = 440 (1.360)coding (405/441 nt) TMARI_RS05125 transposase
* ? NC_021214 1020436 =289 (0.890)9 (0.030) 7/446 19.5 3.1% coding (16/555 nt) TMARI_RS05115 diguanylate cyclase
?NC_021214 = 1020432 284 (0.900)coding (20/555 nt) TMARI_RS05115 diguanylate cyclase
* ? NC_021214 1071755 =356 (1.100)10 (0.030) 7/448 19.6 2.7% coding (662/1527 nt) TMARI_RS05395 FMN‑binding glutamate synthase family protein
?NC_021214 1071819 = 364 (1.150)coding (726/1527 nt) TMARI_RS05395 FMN‑binding glutamate synthase family protein
* ? NC_021214 1458116 =310 (0.960)8 (0.030) 6/434 19.9 2.8% coding (199/333 nt) TMARI_RS07355 anti‑sigma factor antagonist
?NC_021214 1458229 = 262 (0.850)coding (312/333 nt) TMARI_RS07355 anti‑sigma factor antagonist
* ? NC_021214 = 447895364 (1.130)10 (0.030) 6/436 19.9 2.9% coding (682/1308 nt) TMARI_RS02170 oxidoreductase
?NC_021214 = 448238 316 (1.030)coding (339/1308 nt) TMARI_RS02170 oxidoreductase
* ? NC_021214 1327950 =342 (1.060)9 (0.030) 6/438 20.0 2.8% coding (408/762 nt) TMARI_RS06635 ABC transporter permease
?NC_021214 1328134 = 292 (0.940)coding (592/762 nt) TMARI_RS06635 ABC transporter permease
* ? NC_021214 = 1519042244 (0.750)6 (0.020) 5/436 20.7 2.5% coding (203/225 nt) TMARI_RS07775 hypothetical protein
?NC_021214 = 1519047 233 (0.760)coding (198/225 nt) TMARI_RS07775 hypothetical protein
* ? NC_021214 157995 =339 (1.050)9 (0.030) 5/438 20.7 2.7% coding (478/648 nt) TMARI_RS00740 lactate utilization protein
?NC_021214 158204 = 315 (1.020)coding (1857/1872 nt) TMARI_RS00745 DUF4914 domain‑containing protein
* ? NC_021214 = 1488352291 (0.900)6 (0.020) 5/440 20.8 1.9% coding (1240/1296 nt) TMARI_RS07555 protein translocase subunit SecY
?NC_021214 = 1488914 330 (1.060)coding (678/1296 nt) TMARI_RS07555 protein translocase subunit SecY
* ? NC_021214 804804 =395 (1.220)7 (0.020) 4/412 20.8 2.0% coding (554/603 nt) TMARI_RS03980 hypothetical protein
?NC_021214 1044289 = 338 (1.160)intergenic (+117/‑73) TMARI_RS05230/TMARI_RS05235 hypothetical protein/transposase