breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 50 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,726,651 | 0 | T | G | 62.5% | ‑6.5 / 15.3 | 24 | noncoding (2534/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,726,560 | 0 | C | T | 52.6% | ‑3.7 / 24.0 | 19 | noncoding (2625/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,726,555 | 0 | C | A | 50.0% | ‑4.1 / 29.1 | 19 | noncoding (2630/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,726,996 | 0 | A | . | 50.0% | ‑6.9 / 17.2 | 8 | noncoding (2189/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,727,049 | 0 | C | T | 50.0% | ‑6.7 / 10.4 | 13 | noncoding (2136/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,727,046 | 0 | A | T | 46.2% | ‑6.7 / 14.1 | 13 | noncoding (2139/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,727,003 | 1 | . | C | 44.4% | ‑6.9 / 16.9 | 9 | noncoding (2182/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 164,569 | 0 | C | G | 41.9% | 25.2 / 19.1 | 43 | intergenic (+35/‑161) | hrpB/mrcB | RNA‑dependent NTPase HrpB/peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcB |
* | NC_000913 | 2,727,031 | 0 | T | C | 41.7% | ‑6.4 / 10.5 | 12 | noncoding (2154/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,727,033 | 0 | C | A | 41.7% | ‑6.4 / 11.4 | 12 | noncoding (2152/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,727,035 | 0 | G | T | 41.7% | ‑6.4 / 11.5 | 12 | noncoding (2150/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 2,727,048 | 0 | A | G | 38.5% | ‑6.7 / 10.3 | 13 | noncoding (2137/2904 nt) | rrlG | 23S ribosomal RNA |
* | NC_000913 | 224,771 | 0 | C | G | 36.6% | 1741.8 / inf | 979 | noncoding (1001/1542 nt) | rrsH | 16S ribosomal RNA |
* | NC_000913 | 361,311 | 0 | C | A | 35.6% | 216.0 / 36.2 | 173 | E184* (GAA→TAA) | lacA | galactoside O‑acetyltransferase |
* | NC_000913 | 624,598 | 0 | A | T | 34.4% | 92.3 / 14.5 | 65 | intergenic (‑88/‑287) | fepB/entC | ferric enterobactin ABC transporter periplasmic binding protein/isochorismate synthase EntC |
* | NC_000913 | 843,216 | 0 | G | T | 33.3% | 127.4 / 19.3 | 76 | M295I (ATG→ATT) | rlmF | 23S rRNA m(6)A1618 methyltransferase |
* | NC_000913 | 361,320 | 0 | C | A | 31.1% | 282.1 / 46.2 | 177 | V181F (GTT→TTT) | lacA | galactoside O‑acetyltransferase |
* | NC_000913 | 4,296,060 | 0 | C | T | 30.2% | 192.8 / 128.2 | 149 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
* | NC_000913 | 2,185,429 | 0 | T | G | 29.3% | 239.0 / 40.1 | 133 | intergenic (‑128/+95) | thiM/rcnR | hydroxyethylthiazole kinase/DNA‑binding transcriptional repressor RcnR |
* | NC_000913 | 843,202 | 0 | G | T | 28.7% | 150.0 / 18.7 | 80 | A291S (GCC→TCC) | rlmF | 23S rRNA m(6)A1618 methyltransferase |
Marginal new junction evidence (lowest skew 10 of 121 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1287084 | 287 (1.890) | 43 (0.330) | 11/240 | 3.6 | 14.8% | noncoding (153/171 nt) | rttR | small RNA RttR |
? | NC_000913 | 1287557 = | 251 (1.930) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | NC_000913 | 4037260 = | 8 (0.050) | 15 (0.130) +35 bp |
9/210 | 3.7 | 15.7% | noncoding (1/76 nt) | alaT | tRNA‑Ala |
? | NC_000913 | = 4175818 | 207 (1.370) | noncoding (65/76 nt) | thrT | tRNA‑Thr | |||||
* | ? | NC_000913 | = 3815858 | 122 (0.800) | 15 (0.110) | 11/260 | 3.9 | 11.9% | intergenic (‑90/+5) | pyrE/rph | orotate phosphoribosyltransferase/truncated RNase PH |
? | NC_000913 | 3815941 = | 109 (0.770) | coding (609/687 nt) | rph | truncated RNase PH | |||||
* | ? | NC_000913 | 375023 = | 210 (1.390) | 13 (0.120) | 7/196 | 4.0 | 7.3% | intergenic (+142/‑436) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377530 = | 182 (1.720) | intergenic (+219/+5) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 1286708 = | 268 (1.770) | 25 (0.190) | 9/238 | 4.1 | 6.4% | intergenic (+182/‑1) | narI/ychS | nitrate reductase A subunit gamma/putative uncharacterized protein YchS |
? | NC_000913 | = 1287241 | 507 (3.940) | intergenic (‑5/+3) | rttR/tyrV | small RNA RttR/tRNA‑Tyr | |||||
* | ? | NC_000913 | = 1907629 | 275 (1.810) | 25 (0.160) | 11/280 | 4.2 | 8.3% | intergenic (‑38/+632) | yobF/yebO | DUF2527 domain‑containing protein YobF/uncharacterized protein YebO |
? | NC_000913 | 2101749 = | NA (NA) | noncoding (1195/1195 nt) | IS5 | repeat region | |||||
* | ? | NC_000913 | = 1907489 | 367 (2.420) | 25 (0.160) | 11/280 | 4.2 | 6.4% | coding (103/144 nt) | yobF | DUF2527 domain‑containing protein YobF |
? | NC_000913 | = 1979270 | NA (NA) | noncoding (1/768 nt) | IS1 | repeat region | |||||
* | ? | NC_000913 | 225500 = | 279 (1.840) | 13 (0.110) +35 bp |
7/210 | 4.3 | 6.7% | noncoding (1/76 nt) | alaV | tRNA‑Ala |
? | NC_000913 | = 4175818 | 207 (1.370) | noncoding (65/76 nt) | thrT | tRNA‑Thr | |||||
* | ? | NC_000913 | = 375165 | 242 (1.600) | 9 (0.090) | 6/196 | 4.4 | 4.9% | intergenic (+284/‑294) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | = 377487 | 183 (1.730) | intergenic (+176/+48) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 375124 = | 241 (1.590) | 19 (0.180) | 6/196 | 4.4 | 9.8% | intergenic (+243/‑335) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377530 = | 183 (1.730) | intergenic (+219/+5) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB |