Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F14 I0 R1 206 76.7 3255580 98.8% 3216513 146.3

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCC  >  NC_002947_CJ‑RC/5677417‑5677706
                                                                                                                                                       |                                                                                                                                            
CGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GG                                                                                                                                             <  NB501016:282:H2V7GAFX2:4:11508:21856:2300/151‑1 (MQ=60)
         CCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTAGAAGCCCTATAAAGACACAAAGCCCCTCCGGCAGATAATTTTTTTATATATAAAAGTGCGGCCTTTTGTCGTGATACGGCCGCAAAG‑C‑Gggccccgagg                                                                                                                                    >  NB501016:282:H2V7GAFX2:2:11202:19332:17748/1‑141 (MQ=49)
          CCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGT                                                                                                                                    >  NB501016:282:H2V7GAFX2:3:21603:7821:1276/1‑151 (MQ=60)
               GCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCA                                                                                                                               <  NB501016:282:H2V7GAFX2:2:21205:6052:8058/151‑1 (MQ=60)
                  GCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAG                                                                                                                              <  NB501016:282:H2V7GAFX2:1:21109:24519:13319/149‑1 (MQ=60)
                       CCCGCACGGGCTCATAGAAGACCCGAGGGGGCGGCTCCGCAGCCCTACCACCACACACGGCCGCTCCTCCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAG                                                                                                                       <  NB501016:282:H2V7GAFX2:4:21605:7289:6286/151‑1 (MQ=60)
                       CCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAG                                                                                                                       <  NB501016:282:H2V7GAFX2:3:11511:24031:10282/151‑1 (MQ=60)
                       CCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAG                                                                                                                       <  NB501016:282:H2V7GAFX2:3:11510:2706:16664/151‑1 (MQ=60)
                         CGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATGTCTTCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAC‑CGGCCCCCTCGGTTTTCAGCTGGCAGA                                                                                                                      >  NB501016:282:H2V7GAFX2:4:21412:9945:17113/1‑150 (MQ=60)
                             AGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTA                                                                                                                 <  NB501016:282:H2V7GAFX2:3:11501:12261:12291/151‑1 (MQ=60)
                               GGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCGGAAATTACT                                                                                                               <  NB501016:282:H2V7GAFX2:2:11302:22991:18556/151‑1 (MQ=60)
                                        AAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCGGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTTGCAGAAATTACTTCTGCTTC                                                                                                       <  NB501016:282:H2V7GAFX2:1:21101:11992:10750/150‑1 (MQ=60)
                                          cacccgcgggggctgctgccccccccaaccaccacacaccgccgcccctgccgacAATTTCTTGATCCCAACCGGAGCGGCCTTGTGGCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACA                                                                                                    <  NB501016:282:H2V7GAFX2:1:11301:4195:4950/96‑1 (MQ=60)
                                               GAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTC                                                                                               <  NB501016:282:H2V7GAFX2:1:11109:24813:9970/151‑1 (MQ=60)
                                                AGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCG                                                                                              <  NB501016:282:H2V7GAFX2:1:21102:15140:5808/151‑1 (MQ=60)
                                                  GGGGCTGCTGCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGAC                                                                                            <  NB501016:282:H2V7GAFX2:4:21605:11175:9511/151‑1 (MQ=60)
                                                         CTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGAAAGGGCCGCAAAG‑CGGCCCCCTCGGGTTTCAGCTGGCAGAAATTACTTCAGCTTAACCCCATCGACACGGAT                                                                                      >  NB501016:282:H2V7GAFX2:4:21609:16380:18988/1‑150 (MQ=60)
                                                           TCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGCAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATA                                                                                     <  NB501016:282:H2V7GAFX2:1:11211:4356:10089/149‑1 (MQ=60)
                                                            CGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTTATAGGGCCGCAAAGCC‑GCCCCCGCGGTTTTCAGCTGGCAGAAAataaatctggatgacaccttcgacacagatttcc                                                                                  >  NB501016:282:H2V7GAFX2:2:21103:23998:15193/1‑117 (MQ=60)
                                                              CAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAG                                                                                <  NB501016:282:H2V7GAFX2:4:21504:5392:12656/151‑1 (MQ=60)
                                                               AGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGCCACTGATATCCAGG                                                                               >  NB501016:282:H2V7GAFX2:1:21104:12310:6876/1‑151 (MQ=60)
                                                                       CACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGT                                                                       >  NB501016:282:H2V7GAFX2:3:11602:19824:1331/1‑151 (MQ=60)
                                                                       CACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGT                                                                       >  NB501016:282:H2V7GAFX2:1:21103:9019:13401/1‑151 (MQ=60)
                                                                       CACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGG                                                                        <  NB501016:282:H2V7GAFX2:3:11607:10546:3872/150‑1 (MQ=60)
                                                                         CGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATGTCTACAGGAGCGGCCTTGTGTCGTGGTAGGGCCGCAAAG‑C‑Ggcccccgcggttttgaggtgggagaaaattaatctgggtgacaccatagacacgggtttacaggtgcccggtgt                                                                    >  NB501016:282:H2V7GAFX2:1:11309:24964:3786/1‑77 (MQ=60)
                                                                         CGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGGTAGGGCCGCAAAG‑CGGCCCCCTCGGGTTTCAGCTGGCAGAAATTAATTATGCTTAACACATTCGACACTGATATACAGGTCCAGGGTGT                                                                     >  NB501016:282:H2V7GAFX2:4:21601:14767:13152/1‑151 (MQ=60)
                                                                            CACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCG                                                                  <  NB501016:282:H2V7GAFX2:1:21209:5370:4842/151‑1 (MQ=60)
                                                                                        cctgtagagatcaagaaatTCTCTGCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACAC                                                                                          <  NB501016:282:H2V7GAFX2:3:11512:19203:17680/96‑1 (MQ=45)
                                                                                        CCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACC                                                        <  NB501016:282:H2V7GAFX2:3:21402:4634:5871/149‑1 (MQ=60)
                                                                                         CTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATTTACAGGTCCAGGGTCTGCGAAGTAGCACCCGG                                                     >  NB501016:282:H2V7GAFX2:1:21304:5891:11270/1‑151 (MQ=60)
                                                                                         CTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG                                                     >  NB501016:282:H2V7GAFX2:1:21301:25767:12809/1‑151 (MQ=60)
                                                                                         CTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGG                                                     >  NB501016:282:H2V7GAFX2:3:21406:18369:13119/1‑151 (MQ=60)
                                                                                            CAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATACAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCC                                                  >  NB501016:282:H2V7GAFX2:3:11508:13666:17124/1‑151 (MQ=60)
                                                                                              GAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGG                                                    >  NB501016:282:H2V7GAFX2:3:11406:18650:19945/1‑147 (MQ=60)
                                                                                                GAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTG                                              <  NB501016:282:H2V7GAFX2:4:21403:25900:5967/151‑1 (MQ=60)
                                                                                                 AATTTCTTGAGCTCTACCGGCGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTG                                              <  NB501016:282:H2V7GAFX2:1:21301:1661:3634/150‑1 (MQ=60)
                                                                                                         GATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGA                                       >  NB501016:282:H2V7GAFX2:4:11512:8136:5505/1‑149 (MQ=60)
                                                                                                            CTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCG                                  >  NB501016:282:H2V7GAFX2:2:11204:1531:12084/1‑151 (MQ=60)
                                                                                                                ACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCA                              <  NB501016:282:H2V7GAFX2:4:11506:11576:14987/151‑1 (MQ=60)
                                                                                                                ACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGT                                 <  NB501016:282:H2V7GAFX2:3:21407:4114:10557/148‑1 (MQ=60)
                                                                                                                     AGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTT                          >  NB501016:282:H2V7GAFX2:2:11207:8463:19371/1‑150 (MQ=60)
                                                                                                                       CGGCCTTGTGTCGTGATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAG                       <  NB501016:282:H2V7GAFX2:4:11402:11059:10891/151‑1 (MQ=60)
                                                                                                                                     GATAGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGT         >  NB501016:282:H2V7GAFX2:3:21411:26124:19213/1‑151 (MQ=60)
                                                                                                                                      ATAGGGCAGAAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTT        <  NB501016:282:H2V7GAFX2:3:21411:26595:15380/151‑1 (MQ=60)
                                                                                                                                        AGGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTG       <  NB501016:282:H2V7GAFX2:1:11302:9905:15410/150‑1 (MQ=60)
                                                                                                                                         GGGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGA      >  NB501016:282:H2V7GAFX2:4:21401:2075:5492/1‑150 (MQ=60)
                                                                                                                                          GGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAG    <  NB501016:282:H2V7GAFX2:3:11503:21293:2145/151‑1 (MQ=60)
                                                                                                                                          GGCCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGA      >  NB501016:282:H2V7GAFX2:1:21104:1736:19583/1‑149 (MQ=60)
                                                                                                                                            CCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCC  >  NB501016:282:H2V7GAFX2:4:21408:8858:15328/1‑151 (MQ=60)
                                                                                                                                            CCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCC  <  NB501016:282:H2V7GAFX2:3:11607:5331:13886/151‑1 (MQ=60)
                                                                                                                                            CCGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCC  >  NB501016:282:H2V7GAFX2:4:21609:15563:10996/1‑151 (MQ=60)
                                                                                                                                             CGCAAAG‑CGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCC  >  NB501016:282:H2V7GAFX2:1:11112:11569:19179/1‑150 (MQ=60)
                                                                                                                                                       |                                                                                                                                            
CGGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAG‑C‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGCC  >  NC_002947_CJ‑RC/5677417‑5677706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑