Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F13 I0 R1 203 85.6 3649704 98.8% 3605907 146.1

Breseq alignment

N/A

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGC  >  NC_002947_CJ‑RC/5677426‑5677697
                                                                                                                                             |                                                                                                                                   
CCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGG                                                                                                                            >  NB501016:282:H2V7GAFX2:1:11207:1260:18533/1‑151 (MQ=60)
          CTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTG                                                                                                                  >  NB501016:282:H2V7GAFX2:3:21608:20891:4183/1‑151 (MQ=60)
                             TGAAGTTCCGTGGGGTCTGCTTCGCAGCCCTTTCACGACACAAGGCCGCTCGTGCAGATAATTTCTTTTACTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCT                                                                                                <  NB501016:282:H2V7GAFX2:3:11512:11567:7397/150‑1 (MQ=60)
                               AAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCA                                                                                             <  NB501016:282:H2V7GAFX2:4:11407:23383:6655/151‑1 (MQ=60)
                                AGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGC                                                                                                                <  NB501016:282:H2V7GAFX2:1:11110:16651:18938/131‑1 (MQ=60)
                                   ACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACAC                                                                                          <  NB501016:282:H2V7GAFX2:2:11201:8599:9732/150‑1 (MQ=60)
                                       cgggggctgcttggctgccctagaactaaacaaggccgctcctgcttatattttcttttTCTCTACAGTAGGGGCCTTGTGTCGTGATAGGGCGGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTC                                                                                      <  NB501016:282:H2V7GAFX2:1:21308:4480:8008/91‑1 (MQ=60)
                                         ggggctccttccctcccaaaccaccACACACGGCCGCTCCTCCAGATAATTTCTTCTTCTCTCCCGGCGCGGCCTTGTGTCGTTATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGAC                                                                                   <  NB501016:282:H2V7GAFX2:1:11312:26197:6696/126‑1 (MQ=60)
                                         GGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGAC                                                                                   <  NB501016:282:H2V7GAFX2:1:11107:16794:17021/151‑1 (MQ=60)
                                          GGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGGGCGGCCTTGTGTCGTGGTAGGGCCGCAAAGCGGCCCCCGCGGGTTTGAGCTGGCAGCAATTACTTGTGGTTCACACCTTCGACC                                                                                  >  NB501016:282:H2V7GAFX2:1:21204:7490:3478/1‑151 (MQ=60)
                                          GGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACA                                                                                  <  NB501016:282:H2V7GAFX2:2:11205:21319:10941/151‑1 (MQ=60)
                                          GGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCGCGGGTTTCCGCTGGCAGAAATTACTTGTGGGTCACACCTTCGgcc                                                                                  >  NB501016:282:H2V7GAFX2:4:21505:2686:6669/1‑148 (MQ=60)
                                                  TCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATA                                                                            <  NB501016:282:H2V7GAFX2:4:21507:5224:10922/149‑1 (MQ=60)
                                                     CAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGGTAGGGCCGCAAAGCGGCCCCCGCGGGTTTGAGCTGGCAGAAATTACTTCTGGT                                                                                                >  NB501016:282:H2V7GAFX2:2:11306:21726:15657/1‑126 (MQ=60)
                                                     CAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCT                                                                                                   >  NB501016:282:H2V7GAFX2:3:21512:19302:10923/1‑123 (MQ=60)
                                                      AGCCCTACCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGG                                                                      <  NB501016:282:H2V7GAFX2:2:21106:12356:17059/151‑1 (MQ=60)
                                                              CACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGT                                                              >  NB501016:282:H2V7GAFX2:3:21607:15462:13732/1‑151 (MQ=60)
                                                              CACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGG                                                               <  NB501016:282:H2V7GAFX2:4:21510:23077:11247/150‑1 (MQ=60)
                                                              CACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGAAATCCAGGTCCAGGGT                                                              >  NB501016:282:H2V7GAFX2:4:21409:19877:13449/1‑151 (MQ=60)
                                                                   CACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTGAGGTGGCAGAAATTTGTTGTGGTTGACACCTTGGACACTGATATGCAGGTCCAGGGTGTGCG                                                         >  NB501016:282:H2V7GAFX2:2:21309:2454:17155/1‑151 (MQ=60)
                                                                   CACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGCCACTGATATCCAGGTCCAGGGGCTGCG                                                         >  NB501016:282:H2V7GAFX2:1:21207:17863:12251/1‑151 (MQ=60)
                                                                   CACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCG                                                         <  NB501016:282:H2V7GAFX2:4:11609:17049:11896/151‑1 (MQ=60)
                                                                   CACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCG                                                         >  NB501016:282:H2V7GAFX2:1:21104:13168:10326/1‑151 (MQ=60)
                                                                     CAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGA                                                        >  NB501016:282:H2V7GAFX2:2:21301:16652:11593/1‑150 (MQ=60)
                                                                     CAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGA                                                        >  NB501016:282:H2V7GAFX2:2:11301:4736:6338/1‑150 (MQ=60)
                                                                     CAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGAAATACAGG                                                                      >  NB501016:282:H2V7GAFX2:3:11407:6168:13717/1‑136 (MQ=60)
                                                                       AGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGT                                                     <  NB501016:282:H2V7GAFX2:3:21405:22047:5252/151‑1 (MQ=60)
                                                                                         ATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAT                                   <  NB501016:282:H2V7GAFX2:2:11309:16953:10930/151‑1 (MQ=60)
                                                                                           TTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATG                                  <  NB501016:282:H2V7GAFX2:1:11209:12766:18303/150‑1 (MQ=60)
                                                                                           TTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGA                                    <  NB501016:282:H2V7GAFX2:1:21210:2187:6886/148‑1 (MQ=60)
                                                                                               TGATCTCTACAGGAGCGGCCTTGTGTGGTGATAGGGCCGCAAAGCGGCCCCCGCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCC                                <  NB501016:282:H2V7GAFX2:2:21306:23502:8766/148‑1 (MQ=60)
                                                                                                   CTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGG                                                               >  NB501016:282:H2V7GAFX2:3:11402:18179:2910/1‑113 (MQ=60)
                                                                                                       ACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTC                      <  NB501016:282:H2V7GAFX2:3:11403:8175:14360/150‑1 (MQ=60)
                                                                                                       ACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTC                      <  NB501016:282:H2V7GAFX2:2:11312:5677:18013/150‑1 (MQ=60)
                                                                                                       ACAGGAGCGGCCTTGTGTCGTGATAGGGCCCCAAAGCGGCCCCCTCGGTTTTCAGCTGGCACAAATTACTACTGCATCACACCTTCCACACTGAAAACCAGGACCAGGGTCTCCCAAGTAGCACCCCGGCCATAGATGCCGAACTCGTTC                      >  NB501016:282:H2V7GAFX2:1:21205:18907:4808/1‑150 (MQ=53)
                                                                                                         AGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGG                   <  NB501016:282:H2V7GAFX2:2:21208:18908:10606/151‑1 (MQ=60)
                                                                                                            AGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTC                <  NB501016:282:H2V7GAFX2:1:11209:11939:12576/151‑1 (MQ=60)
                                                                                                                     GTTTCGTTATTGGGCCGCAAAGCGGCCCCCTCTGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGT        <  NB501016:282:H2V7GAFX2:1:11209:1760:9302/150‑1 (MQ=60)
                                                                                                                       GTCGTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGT     >  NB501016:282:H2V7GAFX2:1:11203:1766:15064/1‑151 (MQ=60)
                                                                                                                          GTGATAGGGCCGCAAAGCGGCCCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGC  >  NB501016:282:H2V7GAFX2:3:11403:18261:7223/1‑151 (MQ=60)
                                                                                                                                             |                                                                                                                                   
CCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCGCCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGC  >  NC_002947_CJ‑RC/5677426‑5677697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑