Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F34 I0 R1
|
25 |
22.0 |
717516 |
96.1% |
689532 |
148.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP009273 |
2,719,426 |
T→C |
59.4% |
intergenic (‑321/+2) |
kgtP ← / ← rrfG |
alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009273 | 2,719,426 | 0 | T | C | 59.4%
| 2.5
/ 12.1
| 15 | intergenic (‑321/+2) | kgtP/rrfG | alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon |
| Reads supporting (aligned to +/- strand): ref base T (4/2); new base C (4/5); total (8/7) |
| Fisher's exact test for biased strand distribution p-value = 6.08e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01 |
GGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCA > CP009273/2719293‑2719547
|
ggCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTc > 2:320193/1‑149 (MQ=255)
gCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCTAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTcc < 1:103307/149‑1 (MQ=255)
ggggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGGAGTTCCCTAccct > 2:255642/1‑149 (MQ=255)
aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa < 1:117358/149‑1 (MQ=255)
gggAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACg > 2:221109/1‑149 (MQ=255)
atgcgacgctgcgCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACGACCATCGGCGCTACTGAGttt > 2:113390/13‑149 (MQ=21)
taAGACGCGCAGCGTCGCATCAGGCATTTTTTTTTTTTTTTATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGGGACCCCGCACTACCATCGGCGCTTCGGCGTTTc > 1:228226/1‑149 (MQ=21)
gcgcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCGTAATTTGATGCCTGCCAGGTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCCCTATGGCGTTCCACtccg > 1:38855/1‑146 (MQ=25)
cctgtcttCGCATCAGGCATTTTTTTCTGCGCCAATTCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTg < 1:289834/142‑1 (MQ=255)
tCAGGCATTTTTTTCGGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCCACGGCGTTTCACTTCTGAGTTCGGCATgg < 2:216541/149‑1 (MQ=255)
aTTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAg < 1:109875/149‑1 (MQ=255)
tttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTg < 1:241314/149‑1 (MQ=255)
tttgccgcgcGACTGCAGAAGGGCCAGCCGTCAGGATGGCCTCTCGCATAACTCGATGCCTGGCAGTTCCCTACTCGCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTgg < 1:91036/143‑1 (MQ=17)
tGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGacca > 2:179394/1‑149 (MQ=255)
tCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCa > 2:325757/1‑149 (MQ=255)
|
GGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCA > CP009273/2719293‑2719547
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
