Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A18 F25 I40 R1 4 120.6 3921210 96.6% 3787888 148.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP009273 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,719,4260TC96.6% 243.0 / ‑2.1 89intergenic (‑321/+2)kgtP/rrfGalpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (51/34);  total (53/36)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

GTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGC  >  CP009273/2719282‑2719568
                                                                                                                                                |                                                                                                                                               
gTAGGGAAGTGGGCAAGTGGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATg                                                                                                                                             <  2:710862/149‑1 (MQ=255)
       aGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCa                                                                                                                                      <  2:1261524/149‑1 (MQ=255)
        gTGGGCAAGTTGGCGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAg                                                                                                                                     >  1:1341684/1‑149 (MQ=255)
          gggCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGCCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGtt                                                                                                                                   <  1:762460/149‑1 (MQ=255)
           ggCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGAGGCGTAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGGTc                                                                                                                                  >  1:559472/1‑149 (MQ=255)
            gCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTcc                                                                                                                                 >  1:791945/1‑149 (MQ=255)
               tgTTGGGGCTGTCGTATCCGTTGCCGACTATGGCATATGTGGGAGTATAAGACGCGCGGCGTCGCATCAGGTGTTTTTTTCTGCGCTAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTa                                                                                                                              <  2:1345141/148‑1 (MQ=21)
                   ggggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTGCGCATAATTCGATGCCTGGCAGTTCCCTActct                                                                                                                          >  2:504751/1‑149 (MQ=255)
                         gcctaaTCCGTTGCTCAACCTGCCATATGCGGGCATAAAACACGCGCAACTTCCCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTCCGCATAATTCGATGCCTAGCAGTTCCCTACTCCCGCATg                                                                                                                    <  2:412696/144‑1 (MQ=17)
                         gCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGGTGGCCTTTCGCATAATTCGGTGCCTGGCAGTTCCCTACTCTCGCATg                                                                                                                    >  1:550125/1‑149 (MQ=255)
                           cGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGTATggg                                                                                                                  <  2:1643725/149‑1 (MQ=255)
                           cGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATggg                                                                                                                  <  1:1359330/149‑1 (MQ=255)
                            gTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTCCTCTCGCCTgggg                                                                                                                 >  1:1505656/1‑149 (MQ=255)
                            gTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgggg                                                                                                                 >  1:914930/1‑149 (MQ=255)
                             tATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGga                                                                                                                >  1:1737938/1‑149 (MQ=255)
                             tATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGga                                                                                                                <  2:1742548/149‑1 (MQ=255)
                              aTCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGAGgac                                                                                                               >  1:1903350/1‑148 (MQ=255)
                                ccGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCAGCAGGCATTTTTTTCTGAGCCGATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAGTTCGATGCCTGGCAGTTCCCGACTCTTCCATGGGGAGAc                                                                                                             >  2:240034/1‑149 (MQ=25)
                                  gTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTccc                                                                                                                                >  2:1772240/1‑128 (MQ=255)
                                  gTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTccc                                                                                                                                <  1:1772240/128‑1 (MQ=255)
                                  gTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGAccc                                                                                                           >  1:1253885/1‑149 (MQ=255)
                                   ttGCTGAATCTGGCATATGTGGGAGGATAAGACGCGCCGCGTCGCATCAGGCATTTTTTTCTGCGCTTATGCAGAAAGGCCATCCGTCAGGATGGCCATTCGCATAATTCGGTGCCTGGCAGTTCCCTACTCTCGCATGGGGGGAcccc                                                                                                          >  1:868287/1‑149 (MQ=25)
                                      cTGAATCTGGCATATGTGGGAGTATAAAACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCCATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCcaca                                                                                                       >  1:484026/1‑149 (MQ=255)
                                         aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGCGACCCCACACtc                                                                                                    >  2:1379728/1‑148 (MQ=255)
                                               gCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCg                                                                                              >  1:1619165/1‑149 (MQ=255)
                                                 atatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgc                                                                                            <  1:402342/149‑1 (MQ=255)
                                                  tatGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCTAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCGCACGACCCTCgggg                                                                                           >  2:26813/1‑147 (MQ=25)
                                                   atGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAGTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCCGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcgc                                                                                          >  1:1737792/1‑149 (MQ=255)
                                                   atGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGgcgc                                                                                          >  1:446166/1‑149 (MQ=255)
                                                     gtgGGAGTATAAGACGCGCAGCGTCGCATCAGGCACTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACCCTCGCATGGGGAGCCCCCCCACTACCATCGGCGCTa                                                                                        >  2:336346/1‑149 (MQ=25)
                                                     gggggaagtgaaGACGCGCAGCGTCGCATCAGGCATTCTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTa                                                                                        <  1:798032/139‑1 (MQ=25)
                                                     gggggAGGATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGTCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTa                                                                                        <  1:183754/147‑1 (MQ=255)
                                                        ggAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACgg                                                                                     >  1:892325/1‑149 (MQ=255)
                                                         gAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGGCCCCACACTACCCTCGGCGCTACCG‑c                                                                                   >  1:83877/1‑149 (MQ=255)
                                                             ataAGACGCGCAGCGTCGCAGCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTCCTCTCGTATGAGGAGACCCCACACTACCATCGGCGCTACGG‑CGttt                                                                               <  2:723780/149‑1 (MQ=25)
                                                              tgcgacgctgcgCGTCGCATCAGGCATTTTTTTCTTCGTCATTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTAACTAGTCTCGCCTGGCGAGTCCCCACACCACCATCGGCGCTACGG‑CGTTTc                                                                              >  2:699109/12‑149 (MQ=18)
                                                              taAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTTCTCCAATGCAAAAAAGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTc                                                                              >  2:1231961/1‑149 (MQ=255)
                                                              taAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCACGCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGGTTc                                                                              >  1:1840811/1‑149 (MQ=25)
                                                                    gcgcAGCGTCGCATCAGGCATTTTTTTTTTTTTTTTTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCGTAATTGGATGCCTGGCAGGTCCCTCCTCTCGCTTGGGGAGGCCCCACACTACCATCGGCGCTTCGG‑CGTTTCCCTTCt                                                                        >  2:301511/1‑149 (MQ=18)
                                                                    gcgcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCt                                                                        <  2:422116/149‑1 (MQ=255)
                                                                    gcgcAGCGTCGCATCAGGCATTTTTTTCTGAGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCACACACTACCATAGGCGATACGG‑AGATTCACTTCt                                                                        >  2:1354093/1‑149 (MQ=25)
                                                                     cgcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTg                                                                       <  1:1951143/149‑1 (MQ=255)
                                                                      gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGa                                                                      <  2:547029/149‑1 (MQ=255)
                                                                      gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTATCGCATGGTTAAACTCCACACTACAGTCGGCGCTACGG‑CGTTTCACTTCTGa                                                                      >  1:1517013/1‑149 (MQ=21)
                                                                          cggcGCTTCTGGCATTTTTTTGTGCGCCAAGGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGGCCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTc                                                                  <  2:1622241/146‑1 (MQ=21)
                                                                          cGTCGCGTCCGGCATTTTTTTCCGCGCCAATGCAAATAGGCCATCCGTCAGGATTGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTc                                                                  <  2:1370304/149‑1 (MQ=21)
                                                                           gTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCg                                                                 <  2:635024/149‑1 (MQ=255)
                                                                            tCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCgg                                                                <  1:1400600/149‑1 (MQ=255)
                                                                              gCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTACGG‑CGTTTCACTTCTGAGttaagta                                                              >  2:488554/1‑144 (MQ=255)
                                                                               cATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCAt                                                             >  1:27344/1‑149 (MQ=255)
                                                                                     gCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCAGggggcc                                                       >  2:573275/1‑147 (MQ=255)
                                                                                      cATTTTTTTCTGCGTCAATTCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGACAGTTCCCTACTCTCGCATGGGGAGACCCCACACTAACATCGGCGCTGCGA‑CGTTTCACTTCTGAGTTCGGAATggggctc                                                      >  2:1560550/1‑146 (MQ=21)
                                                                                       aTTTTTTGCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAg                                                     <  1:410038/149‑1 (MQ=255)
                                                                                        tttttttCTGCGCCAATGCAAAAGGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGGAGTTACCTACTCTCGCCTGGGGAGACCCCACACTACCATCGGCGCTGCGG‑CGTTTCACTTCTGAGTTCGGCATGGGGCCAgg                                                    >  1:1061540/1‑149 (MQ=21)
                                                                                            tttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg                                                <  1:67181/149‑1 (MQ=255)
                                                                                            tttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg                                                <  1:504751/149‑1 (MQ=255)
                                                                                            tttCTCCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTggg                                                <  2:449462/149‑1 (MQ=255)
                                                                                              tCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCATTTCTGAGTTCGGCATGGGGTCAGGTGGGac                                              >  1:1360427/1‑149 (MQ=255)
                                                                                                  cgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCATCGGCGCTCCGG‑CGTTTCACTTCTGAGTTCGGAAGGGGGTCAGGTGGGaccacc                                          >  1:934304/1‑149 (MQ=18)
                                                                                                   gccggtGCAATGAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCGGTTCCCTACTCTCGCATGGGGAGACCCCACACTGCCAT‑GGAGGCACGGTCGTTTTTCTTCTGGGATCGGCATGGGGTGAGGTGGGGCAACcg                                         >  2:413026/6‑149 (MQ=12)
                                                                                                   gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg                                         <  1:189329/149‑1 (MQ=255)
                                                                                                   gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCAGCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCACGGGGTCGCGTGGAagcaccc                                         >  2:774575/1‑148 (MQ=18)
                                                                                                       aTGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTGTCCGTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCt                                     >  2:1017221/1‑149 (MQ=255)
                                                                                                          cAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCTTGGGATCAGGTGGGACCACCGCGCTACg                                  <  1:1092609/149‑1 (MQ=255)
                                                                                                           aaaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACgg                                 >  1:1436414/1‑149 (MQ=255)
                                                                                                           aaaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACgg                                 >  1:1227357/1‑149 (MQ=255)
                                                                                                             aaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgcc                               >  1:1297403/1‑149 (MQ=255)
                                                                                                             aaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgcc                               >  1:1060032/1‑149 (MQ=255)
                                                                                                             aaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTGCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCCCCGCGCACCGgcc                               >  1:801939/1‑149 (MQ=17)
                                                                                                               aGGCCATCCGTCAGGATGTCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGCGCTCCACATGTGGTCAGGTGGGACCACCGCGCTACGgccgc                             <  2:914930/149‑1 (MQ=17)
                                                                                                                 gCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGGCAGGTGGGACCACCGCGCTACGGCCGCCa                           >  2:303444/1‑149 (MQ=255)
                                                                                                                  ccATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                          >  1:279565/1‑149 (MQ=255)
                                                                                                                       cGTCAGCATGGGCCTTCGAATAATTCGATGCCGGGCAGATCCCTGGTCTCGCATGGGGTGGCCCCCCGCTACCAGCGGCGCTACTG‑CGGTTGACGTCTGAGTTCGGCATTGGGTCAGGTGGGACCACCGAGCTACGGCCGCCAGGCaaa                     <  2:833645/149‑1 (MQ=2)
                                                                                                                         tCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCCGGCaaact                   >  2:1783623/1‑147 (MQ=255)
                                                                                                                            ccttagCCTCTCGCTTAATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTg                <  2:81274/144‑1 (MQ=255)
                                                                                                                             gATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGt               >  2:1040521/1‑149 (MQ=255)
                                                                                                                             gATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCCACGGCCGCCAGGCAAATTCTGt               >  1:1653124/1‑149 (MQ=255)
                                                                                                                              ttGGCCTTTCGCATCAGGCGCTGCCTGGCAGTCCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCCACGG‑CGTTTCACTTCTTAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGTCGCCAGGCAAATTCTGtt              <  2:1270901/148‑1 (MQ=11)
                                                                                                                              aTGGCCTTTCGCATAATTCGATGCCCGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGtt              <  2:1737792/149‑1 (MQ=255)
                                                                                                                               tGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGttt             <  1:694557/149‑1 (MQ=255)
                                                                                                                                  ccggttgCATAAGTCGATGCCTGGCAGTGCCCTACTCTCGCATGGGGAGTCCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt          <  2:792028/143‑1 (MQ=11)
                                                                                                                                  ccTTTCGCATAATTCGATGCCTGGCAGTCCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt          <  1:26813/149‑1 (MQ=255)
                                                                                                                                    tttCGCATAATTTGATGCCTGCCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACGACCATCGGCGCTACGG‑CGTTTCACTGCTGTGTTCGGCATGGGGGCATGTGGGACCACCGCGCGACGGCCGCTAGGCAAATTCTGTTTTATCa        <  1:1364181/149‑1 (MQ=11)
                                                                                                                                    tttCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTGGGCATGGGGTCCGGTGGGACCACCGCGCTACGGCCGCCCGGCNAATTCCGTTTTCTCa        >  1:1590651/1‑149 (MQ=11)
                                                                                                                                    tttCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCa        <  1:797941/149‑1 (MQ=25)
                                                                                                                                    tttCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCa        >  1:735286/1‑149 (MQ=25)
                                                                                                                                    tttCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCa        >  2:578250/1‑149 (MQ=25)
                                                                                                                                         cATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCg   >  1:1562031/1‑149 (MQ=14)
                                                                                                                                          aTAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTGCACTTCTGAGTTCGGCAGGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGc  >  1:603922/1‑149 (MQ=2)
                                                                                                                                                |                                                                                                                                               
GTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGG‑CGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACCGC  >  CP009273/2719282‑2719568

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A