Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A17 F25 I40 R1	5	43.7	1427114	97.2%	1387154	146.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,719,426	T→C	intergenic (‑321/+2)	kgtP ← / ← rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,719,426	0	T	C	100.0%	117.7 / NA	39	intergenic (‑321/+2)	kgtP/rrfG	alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (24/15); total (24/15)

TGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719285‑2719566

tGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAAGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCCATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGa                                                                                                                                            >  1:59702/1‑144 (MQ=255)

  gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATg                                                                                                                                          <  1:41114/144‑1 (MQ=255)

  gAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATg                                                                                                                                          >  1:423630/1‑144 (MQ=255)

       ggtcAAGTGGGGGAGGCCGCATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTgg                                                                                                                                     <  1:187718/141‑1 (MQ=25)

                   gAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAACAAGCCATCCGCCAGGATGGCCCTTCGCCTAATTCGACGCATggacactccccacg                                                                                                                         >  1:72498/1‑132 (MQ=21)

                    aaGCCGTATCCGTTGCTGAATCTGGCATATGTGGGCGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCa                                                                                                                   >  2:621567/1‑149 (MQ=255)

                       ccGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                                <  2:297847/149‑1 (MQ=255)

                          tATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCCGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCCAAAAGGCCATCCGTCAGGATGGCCTTTCGCCTCATTCGATGCCTGGCAGTTCCCTACTCTCTCATGGGga                                                                                                             >  2:97687/1‑149 (MQ=25)

                            tCCGTTGGTGGATGTGGCGTATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATgg                                                                                                                <  1:153583/144‑1 (MQ=21)

                                 tGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCca                                                                                                      <  2:439127/149‑1 (MQ=255)

                                      aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa                                                                                                 >  2:735520/1‑149 (MQ=255)

                                        tCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcaca                                                                                                    <  1:658420/144‑1 (MQ=255)

                                                      gAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATCATTCGCTGCCTGGCAGTGCCCTACTCTCGCATGGGGAGACCCCACACTACAAGCGGCGCt                                                                                      >  1:85021/1‑144 (MQ=25)

                                                       aGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTTTTTTTTTTTTCCAAGAAGCCATCCGTCAGGATCGCCTTTCGCGTAGTTCGATGCCTCGCAGTTCCCTCCTCTCGCATGAGCAGCCCCCCCACTCCCCTCCgcgcca                                                                                     >  1:275249/1‑142 (MQ=255)

                                                         tataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGGCATCCGTCAGGATGGCCTTTCGCCTAATTCGATGCCTGGCAGTTCCCAACTCTCGCATTGGGAGGCACCACACTTCCAACGGCGCGACGGCGtt                                                                              >  2:204331/1‑149 (MQ=18)

                                                           taAGACGCGCAGCGTCGCATCAGGCATTTTTTTTTTTTTTTTTGCAAAAAGGCCATCCGACAGGATTGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGGGACCCCACACTCCCACCGGCCCTCCgcc                                                                                 >  1:174218/1‑142 (MQ=21)

                                                                 gcgcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCt                                                                      >  2:123755/1‑149 (MQ=255)

                                                                  cggaGCGTCGTATCAGGCTTTTTTTTCTGCGCCAATGCAAAATGGCTATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTg                                                                     <  2:217905/146‑1 (MQ=21)

                                                                  cgcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTg                                                                     >  2:414938/1‑149 (MQ=255)

                                                                   gcAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCCTCGGCGCTACGGCGTTTCACTCCTgc                                                                    >  2:672691/1‑148 (MQ=255)

                                                                    cAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAg                                                                   >  2:680931/1‑149 (MQ=255)

                                                                         tCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCgg                                                              <  2:434495/149‑1 (MQ=255)

                                                                            cATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCAt                                                           >  2:288422/1‑149 (MQ=255)

                                                                                      ttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGt                                                 <  2:200042/149‑1 (MQ=255)

                                                                                                gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATCATTCGATGCCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg                                       >  2:533970/1‑149 (MQ=255)

                                                                                                 ccAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                      >  2:162526/1‑149 (MQ=255)

                                                                                                 ccAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                      <  2:220490/149‑1 (MQ=255)

                                                                                                 ccAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgc                                      <  2:586509/149‑1 (MQ=255)

                                                                                                         aaaaGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgc                              >  2:365760/1‑149 (MQ=255)

                                                                                                               ccATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                        >  2:34841/1‑149 (MQ=255)

                                                                                                                cATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGgccg                            >  1:412518/1‑144 (MQ=255)

                                                                                                                cATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAgg                       >  2:630470/1‑149 (MQ=255)

                                                                                                                 aTCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGc                      >  2:536169/1‑149 (MQ=255)

                                                                                                                    cGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAg                        <  1:84514/144‑1 (MQ=255)

                                                                                                                            tGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGttt           <  2:520850/149‑1 (MQ=255)

                                                                                                                             ggCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGGCAGGCAAATTCt               >  1:302333/1‑144 (MQ=255)

                                                                                                                             ggCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGtttt          <  2:565795/149‑1 (MQ=38)

                                                                                                                               ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAt        <  2:468107/149‑1 (MQ=37)

                                                                                                                                     gCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCCCCGCGCTACAGCCGCCAGGCAAATTCTGTTTTATCAGAcc  >  2:611463/1‑149 (MQ=12)

TGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTATCAGACC  >  CP009273/2719285‑2719566

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 8 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A