Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F32 I0 R1
|
15 |
16.5 |
534746 |
98.2% |
525120 |
146.6 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
CP009273 |
2,719,426 |
T→C |
42.3% |
intergenic (‑321/+2) |
kgtP ← / ← rrfG |
alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | CP009273 | 2,719,426 | 0 | T | C | 42.3%
| 7.0
/ 25.1
| 26 | intergenic (‑321/+2) | kgtP/rrfG | alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon |
| Reads supporting (aligned to +/- strand): ref base T (8/7); new base C (4/7); total (12/14) |
| Fisher's exact test for biased strand distribution p-value = 4.53e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 |
ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT > CP009273/2719280‑2719560
|
acGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATAAGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGa > 2:2349/1‑149 (MQ=255)
tGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGa > 1:83080/1‑144 (MQ=255)
cAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTccc > 2:245078/1‑149 (MQ=255)
tgggAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCATCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGTCATCTGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTActct < 2:59802/148‑1 (MQ=255)
ggAAGCCGTATCCGTTGCTGAATCTGGCCTATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTAc < 1:24855/144‑1 (MQ=255)
acGTATCCGTTGCTGAATCTGGCAAATGTGGGCGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGCTGCCTGGCAGTTCCCTACTCTCg > 1:144067/2‑144 (MQ=255)
tATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGTATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGga < 2:5899/149‑1 (MQ=255)
gCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGTTGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac < 2:89577/149‑1 (MQ=255)
tGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGTTGGTCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCacac < 2:91800/149‑1 (MQ=255)
gAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTTAGGTTGTCCTTTCGCATTATTTGTTGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt < 2:90204/149‑1 (MQ=37)
gAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCAACAGGCAATATAAACAACACCAATACAAAAAGGCCAACCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACt > 2:122272/1‑149 (MQ=25)
aaTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTGCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTa > 2:147463/1‑149 (MQ=255)
aTCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCcac < 1:223807/144‑1 (MQ=255)
ggAGTATAAGACGCGCAGCTTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTTTCGCATGGGGAGACCCCACACTACCATCGGCGCTACgg < 2:166405/149‑1 (MQ=255)
tataAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCATTGCAAAAAGGCCATCCGTCAGGATGGACTTTCGCATAATTTGATGCCTGGCAGTTGCCTACTCTCGCATCGGGAGACCGCACACTACTATCGGCGCTCCg > 1:6896/1‑144 (MQ=21)
cgcgCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtgta > 1:153101/1‑141 (MQ=255)
gTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGAATAGAAAATCTTTACTCTCGCTTGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGa > 1:79989/1‑144 (MQ=25)
tttttttCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATgggg < 1:2057/144‑1 (MQ=255)
cgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCACATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccccc > 2:8699/1‑149 (MQ=255)
cgcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAAATTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGaccacc > 2:140462/1‑149 (MQ=255)
gcCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcg > 2:186409/1‑149 (MQ=255)
gCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAc < 2:134032/149‑1 (MQ=255)
cataaaGTCCACCCGTCAGGATGGCCTTTCGCATAATTCGATGCCTGGCTGTTCCCTCCTCTCGCATGGGGAGACCCCACAGTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACcgcg < 1:177101/141‑1 (MQ=17)
gATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCCTGGGGAGACCCCACACTACCATCGGCGCTCCGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAAt < 1:217418/144‑1 (MQ=255)
ccTTTCGCATAATTCGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTTAGGTGGGACCATCGTGCTACGGCCGCCAGGCAAATTCTGTTTTAt < 2:61687/149‑1 (MQ=255)
tCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTa < 1:109652/144‑1 (MQ=33)
|
ACGTATGGAAGTGGGCAAGTTGGGGAAGCCGTATCCGTTGCTGAATCTGGCATATGTGGGAGTATAAGACGCGCAGCGTCGCATCAGGCATTTTTTTCTGCGCCAATGCAAAAAGGCCATCCGTCAGGATGGCCTTTCGCATAATTTGATGCCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACGGCCGCCAGGCAAATTCTGTTTTAT > CP009273/2719280‑2719560
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
